Re: [Histonet] Ventana temp veriifer slides

I'm doing the even one quarter and the odds the next also. I number the slides in Sharpie to cprrespond to the heat pad # after they are finished and take a photo of them for documentation, then toss the slides. es...I've decided to do the even's 1 quarter and the odds the next quarter (becau

Re: [Histonet] Negative Reagent Control

Our Prayers have been answered!!! >>> Richard Cartun 7/17/2012 7:04 PM >>> Most of you know that I have been advocating for the elimination of the "Negative Reagent Control" when using a non-avidin-biotin detection system (polymer) in immunohistochemical testing. In my opinion, it is a waste o

Re: [Histonet] Leica CV5030 coverslipper issues

If you are using slides with labels (esp. Ventana's), don't think the upgrade will fix it. We tear our hair out most days of the week with our 2 CV5030s. I wish Ventana would just make a coverslipper to use with their labels or change their labels to something thinner. Symphony takes too long to

RE: [Histonet] FW: Redneck Lent

I'm a redneck Catholic too and I heard it weeks ago and thought it was pretty funny. Still doesn't belong on a public forum though. >>> "Britton, Josette C" 3/27/2012 3:16 PM >>> I am a redneck and a catholic and was not offended at all! As a matter of fact it made me hungry! -Original

Re: [Histonet] IHC + control question

My vote has always been for vimentin. See Dako's booklet IHC Staining Methods, 5th Ed, Chapter 19 addresses this. Angie >>> "Chiriboga, Luis" 3/6/2012 9:01 AM >>> Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as

Re: [Histonet] Smad4 (B-8) / DPC4

I've tried to work it up on the XT also with inconsistent, weak results. If you get a reply on this one, let me know. Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 57

Re: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested

I've had a temp, who we interviewed over the phone, come in and sit down at a microtome and create the most horrendous slides I've ever seen. He lasted a week and we sent him back from whence he came. I don't think he was EVER a Histotech or if he was it was many, many moons ago. Point is.he

Re: [Histonet] RE: time off

I've witnessed that granting time by seniority lets you open to abuse. I know of an employee with high seniority who takes the whole week after Christmas every year. Our policy allows only one person off per day per shift. So then no one else ever can take off at Christmastime. Her peers complai

Re: [Histonet] IHC on Bones

pray. >>> "McCabe, Sara J." 12/30/2011 1:02 PM >>> Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confident

[Histonet] CK15 with Ultraview

Is anyone running Cytokeratin 15 on the Ventana Benchmark XT or Ultra platform?? Would you be so kind as to share the protocol?? Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634

[Histonet] c-myc

Does anyone send slides out for c-myc staining by IHC? Where do you send to? Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The

Re: [Histonet] saving slides between levels for IHC?

we do for certain part types. sentinel lymph nodes for melanoma, renal bxs, liver core bxs, prostate needle core bxs. >>> "Harrison, Sandra C." 10/25/2011 1:59 PM >>> Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sa

RE: [Histonet] Cat Scratch fever

I was just watching his show last night! That's entertainment! >>> "Galbraith, Joe" 10/12/2011 10:19 AM >>> No one under 30 would have a clue what that joke means. Lol Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.

Re: [Histonet] (no subject)

ch a ridiculous claim. I always put the opinions and experiences of other Histotechs far ahead of anything I hear from sales reps. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fa

Re: [Histonet] STAIN PRECIPITATE

We've been seeing high background staining on abt 10% of our HPs as well. We use Cellmarques predilute on the XT as well. Been happening for a couple months. >>> "Sara Baldwin/mhhcc.org" 9/23/2011 3:57 PM >>> Hi Histonetters I have the Ventana xt, H pylori from cell marque and my pathologists sa

RE: [Histonet] new antibody lot

yes, they are recommendations. Unless it has changed, I believe it is up to the discretion of your Doctoral Director. >>> Elizabeth Chlipala 9/22/2011 2:07 PM >>> CAP has recommendations from the following paper Recommendations for Improved Standardization of Immunohistochemistry Neal S. Golds

RE: [Histonet] new antibody lot

same here. >>> "McMahon, Loralee A" 9/22/2011 1:43 PM >>> >>> That is what we do as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.

Re: [Histonet] pax2

CC1 standard, 32 min incubation with heat disabled. I use Cellmarques predilute and UltraView DAB detection. >>> Dorothy Glass 7/7/2011 3:28 PM >>> Does anyone have a working protocol for pax2 on the Ventana Ultra or XT? Can you please share on Histonet? Dorothy Glass Supervisor, SEPA LABS Br

Re: [Histonet] Luciferase

You can, but the retrieval involves a young priest and an old priest. ff McAuliffe 6/30/2011 12:42 PM >>> It is a devil to stain. Geoff On 6/30/2011 12:13 PM, Angela Bitting wrote: > ok there is a really BAD religious joke coming soon, isn't there? > > >>>&g

Re: [Histonet] Luciferase

ok there is a really BAD religious joke coming soon, isn't there? >>> Rene J Buesa 6/30/2011 12:00 PM >>> You would have to have an antibody to it and I don't know any one exists, but I can be wrong. René J. --- On Thu, 6/30/11, sgoe...@mirnarx.com wrote: From: sgoe...@mirnarx.com Subject:

Re: [Histonet] Anatomicalalal

Shall we all debate the pronunciation of Periodic acid next ?!?! I've heard it 3 different ways so far in my career. >>> "Breeden, Sara" 6/13/2011 3:03 PM >>> I just LOVE it when I'm right - and it happens so rarely! The consensus of ten responders so far is ANATOMIC. This just makes sense to

[Histonet] Ventana probes

I have a Pathologist asking me to try running Kappa and Lambda by CISH on bone marrow smears. Does anyone have any experience with trying that? Happy strange Tuesday, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be

Re: [Histonet] Safety glasses required for embedding

We went round with a Safety Dept. employee when I first started here about gloves for embedding. Couldn't convince him that we didn't need to wear them. >>> Rene J Buesa 5/5/2011 3:46 PM >>> No. Rerné J. From: Cindy DeRiso To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 5, 2011 3:11

[Histonet] Hep B

I'm looking for hepatitis B positive control tissue. Willing to trade if I have tissue that you might want. Contact me off line. Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214

[Histonet] Ventana Probes resolution

Thanks to all of you who offered your help. I did have a conference call with Ventana this afternoon and all my questions were answered. I'll share with you all what I know so no one else needs to fret needlessly. I didn't receive 2- 4ml vials of EBER Probe. I only received 1 when I ordered i

Re: [Histonet] Ventana Probes

Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts? >>> "Angela Bitting" 4/28/2011 11:03 AM >>> Ok. Ventana is on my last nerve with this new probe configuration of theirs

[Histonet] Ventana Probes

k everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTA

Re: [Histonet] RE: [IHCRG] Ventana EBER DNP

Your sales rep should supply these to you. I'm having problems with filling my ISH Probe Kit. I logged in my EBER Probe. I registered the Probe kit and went to fill it and it only lets me pick from my Fillable Reagents list, not the Fillable probes list. Tech support can't figure out why this

RE: [Histonet] Ventana ultra

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, April 13, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu; barbara.cr...@lpnt.net Subject: Re: [Histonet] Ventana ultra each of the 30 drawers essentially is it's own "run". You don't

Re: AW: [Histonet] Benchmark Ultra question - follow up

> -Ursprüngliche Nachricht- > Von: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun > Lang > Gesendet: Montag, 11. April 2011 18:48 > An: 'Angela Bitting' > Cc: histonet@lists.utsouthwestern.ed

Re: [Histonet] Ventana ultra

if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger

RE: [Histonet] Kappa/Lambda ISH on Ventana

ntana probes is your best bet. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly

Re: [Histonet] reagents with no expiration dates

Call the vendor. Many times they will give you a date based upon the lot number. >>> Michele Carr 3/18/2011 12:49 PM >>> Hi everyone was curious about how to obtain an expiration date for those reagents that don't come with one. I know this is a cap requirement and we are preparing for our firs

[Histonet] S100 A1

I'm looking for a mouse anti human S100 A1 antibody for IHC. My vendor doesn't carry it anymore. Anyone have one that they are using on FFPE tissue? Could you send me ordering info or a copy of your data sheet? Thanks, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist,

RE: [Histonet] negative controls on immunos

Fun no, impractical yes. >>> "Weems, Joyce" 3/2/2011 10:30 AM >>> The CAP guidelines are pretty clear. Copied from latest checklist.. Isn't this fun???! j:>) ANP.22570 Phase IIN/A YES NO Are appropriate negative controls used? NOTE: Negative controls must as

RE: [Histonet] How many tissues an histo tech is suppose to cut per hour?

you all saw the little wink ;) at the end of his paragraph, right >>> "Shirley A. Powell" 3/1/2011 3:01 PM >>> Bet you he already does. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Se

Re: RE: [Histonet] parts for CV5030 glass coverslipper

/supplies, I would also be > interested. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting > Sent: Monday, February 28, 2011 10:55 AM > To: histonet > Subject:

[Histonet] parts for CV5030 glass coverslipper

Does anyone know if you can buy these from someone else besides Leica? My Fisher rep never got back to me. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees

Re: [Histonet] CAP

I did notice CAP inspectors concentrated more on safety this time around. >>> Akemi Allison 2/23/2011 11:50 AM >>> We were given the new CAP checklist. I totally revamped our SOP Manuals, ALL QC forms, etc. to comply. This is the 1st Childrens Hospital CAP inspection I have undergone. We

Re: [Histonet] CAP

We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. >>> Akemi Allison 2/23/2011 9:02 AM >>> Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our s

Re: [Histonet] QIHC

I thought the NSH Self Assessment CD was helpful too. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many

Re: [Histonet] High Complexity Testing

That was a hole in 1, Vikki. Viva la rant!! >>> Victoria Baker 2/8/2011 11:58 AM >>> The performance of ISH/IHC is in many laboratories considered high complexity testing - however - as the technologist doing the work does not make the diagnosis it is considered to be a part of specimen processin

RE: [Histonet] Lab Assistants Embedding

Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, January 21, 2011 8:34 AM To: Mahoney,Janice A; Angela Bitting; histonet@lists.utsouthwestern.edu; histonet-reque...@lists.utsouthwestern.edu Subject: RE: [Histonet]

[Histonet] Lab Assistants Embedding

s for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons we

[Histonet] Changing solutions on VIP processors.

not the case. All opinions are welcome. Thanks, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many el

[Histonet] RE: Special Stain Automation

Just wondering how many people hate the Artisan's plastic well things that you have to clip on the slides?? >>> "Nails, Felton" 12/1/2010 11:06 AM >>> The problem with the Nexus is that you can not do silver and AFB on the same run. We use the Dako artisan with very consistent results and it is

Re: [Histonet] IHC FILD

In my case, it was for my own personal gratification. >>> "Debora Probst" 10/15/2010 1:53 >>> PM >>> Can anyone tell me if once you have taken the IHC certification test and passed dose the administration consider that a specialty field and give you a pay increase? Or is it just for a persons o

[Histonet] SOX-10

Happy Monday Histonetters, I'm wondering if anyone can share a protocol for running SOX-10 on the Ventana BenchmarkXT? (On FFPE tissue) Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged.

[Histonet] Parvovirus B19

Wondering if anyone is running Cellmarques Parvovirus B19 or Toxoplasma Gondii on a Ventana platform and would you mind sharing your protocol? Have a great weekend everyone! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00

Re: [Histonet] Powdered reagent expiration dates

s 3-5 years from the manufacture date.) I was recently told by another tech that she thought 5 years was the shelf life of powders, but I wanted to see if that held true. Moral of the story is that it never hurts to call the manufacturer. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Hist

[Histonet] Joe Myers

Joe, if you're out there, will you give me a call or respond offline to my email ?? My number is 570-214-9634 Thanks, Angie Bitting Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 57

Re: [Histonet] Block disposal

They are classified as Pathological Waste. You should see what your state's rules are regarding Pathological Waste. >>> 6/21/2010 12:33 PM >>> Would blocks be considered biohazard waste for purposes of disposal? I have blocks stored in an off-site facility and am preparing to dispose of the blo

[Histonet] Lab refrigerator

got it and the model #? Thanks and Happy Memorial Day All! Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However

[Histonet] Ventana EMA antibody

Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WA

[Histonet] Artisan

Anyone know the approx. price of Dakos Artisan? Cost per slide? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and

[Histonet] Kaiser Permanente

If someone from Kaiser Permanente is watching the Histonet, would you contact me offline about a piece of Histology equipment. My rep. (and I'm not mentioning the company!!) is telling me you have something that I'm interested in buying, and I'd like your feedback. Thanks,

[Histonet] Staffing for IHC

I'm hoping some of the larger hospitals out there will share their data with me. I'm curious as to how labs are staffed for doing IF/IHC/CISH. A small portion of our staining protocols still require pretreatment by hand, but we run instruments that do the pretreatments on board for the majority o

[Histonet] Tau on the Benchmark XT

Good morning, I'm still looking for somebody who is running Tau on their XT. I'm using Biocare's mouse monoclonal antibody with Ultraview Detection on human brain, but I'm not getting any staining. Anyone else having similar problems? ~A Angela Bitting, HT(ASCP)

[Histonet] Autostainer service

suggestion for a company who will service these instruments or if anyone can remember the name of that company in MD, please contact me. Happy Monday everyone, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone

Re: [Histonet] Floaters in Waterbath

dding molds. ~Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 ! >>> "Jackie M O'Connor" 10/23/2009 10:23 AM >>> Kim wipes seem to pick

[Histonet] anti-Tau on BenchmarkXT

I may have asked this before, but is anyone using BioCare's Tau antibody on their BenchmarkXT?? If so, would you be willing to share your experience with it? Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be

[Histonet] thermometer vendor

Can anyone help me find where to order the short thermometers for use in the floatation baths? Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in

Re: [Histonet] water on slides?

We are having a very similar problem too. We change our alcohols and Histoclear like mad, but are having eosin bleeding out of the sections. I'm starting to wonder if it is the mounting media on our glass coverslipper. I ordered new bottle gaskets, so I guess I'll see what happens the

RE: [Histonet] GMS-Fungus on nails

Just wondering why you use Titebond II? We use plain old Elmer's Glue. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this

[Histonet] Looking for NYU contact

Just wondering if anyone can give me contact information for someone in the Neuropath Lab at NYU? Thanks Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt

Re: [Histonet] Job Openings

We have a part-time dayshift and a full-time night shift opening for a HT. If you're interested here's our web address. www.geisinger.org or call me directly. Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Da

Re: [Histonet] Lean Consultants

Here's the website for consulting group we used: www.sprickstegall.com Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this

[Histonet] SMMS

We've been having trouble getting satisfactory staining for SMMS on our BenchmarkXTs. Is anyone getting a good result running these on the XT platform. If so, would you mind sharing your secret?? Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to

[Histonet] Gram & AFB staining FNA smears

use in our Histology lab. I don't know what method Micro labs use, so I was hoping someone could shed some light on this subject for me. Thanks, as always, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822

[Histonet] lifting tissue from glass slides

Does anyone know of a way to take an old H&E slide and remove a section from it to transfer it to another slide? I thought there was a way to do it with tape, but I can't find any information on the web about it. All I could find was tape to transfer frozen sections. Angela Bitting

Re: [Histonet] Benchmark XT

I just had the same problem with a bad lot of charged slides. It is, indeed, maddening. >>> "Joe Nocito" 4/8/2009 5:08 PM >>> another issue could be the mixer blowing too hard. I've had that happen too. JTT - Original Message - From: "Kari Bradshaw" To: "Dana Spears" ; ; "Phyllis Tha

Re: [Histonet] G 20 MEETING NEWS

Yes please. There are plenty of blogs out there for this kind of thing. >>> Bernie Taupin 4/1/2009 9:53 AM >>> Please refrain from discussing politics on this list. Edwards, R.E. wrote: > Apparently Obama and Brown, with the reluctant compliance of Germany and > > China are aiming to rat

[Histonet] Histos-5

at is the volume of the paraffin tank? Thanks for your help once again, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this

Re: [Histonet] C4d Antibody

We get our from Alpco. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced

Re: [Histonet] Paraffin Block trimming

I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sendi

[Histonet] help with muscle stains

Would anyone like to share their procedures for Cytochrome oxidase(COX), Succinic dehydrogenase (SDH) and NADH-TR with me? I have old, old ones but they are sketchy. Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville

RE: [Histonet] Dako, Thermo and others

Danaher owns them both. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced

RE: [Histonet] Dako, Thermo and others

I'm pretty sure Danaher owns Surgipath now.along with nearly everything else..... Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sendi

Re: [Histonet] BVD Antibody

I'm fighting a compulsion to make a bad underwear joke. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many elec

[Histonet] Pathos/ Milestone products

Does anyone have experiences with Milestone equipment, specifically the new KOS and Pathos Delta? Would you share your comments, pros and cons with me? Feel free to call me on the phone if you don't feel like typing a novel. Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Hist

[Histonet] on-line Histology programs

I have a Lab assistant who would like to become an HT. She is interested in OJTing here with us and taking the required classes on-line so that she can sit for her HT exam. Can anyone recommend a good program for her? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology

Re: [Histonet] Breast tissue

We extended our paraffin infiltration times and that worked out well for us too. >>> 2/10/2009 11:10 AM >>> As long as you smell the clearant in the block then putting the blocks in paraffin longer works. I never found it necessary to roll the fat in paper towel and place the specimen back in par

[Histonet] BioCare rep

Can anyone tell me how to get in contact with Kathy Bowden from Biocare? I left a message at Biocare but she didn't call me back. Maybe one of you has her email or phone number. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academ

[Histonet] perplexed

r were not seated properly on the pad. Help me figure this one out folks!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email Ho

[Histonet] PA job

-214-9634 or send me an email. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced

Re: [Histonet] Slide drying

We use MM24 from Surgipath. We file our slides within 24 hours. I don't know if it will work in 12 hours, but maybe it's worth trying. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-963

Re: [Histonet] protocols on Ventana

From what company do you order your PMS2, MSH2 and MSH6 concentrates? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However

RE: [Histonet] breast bx processing

Ok, first of all, I mis-typed--they are loaded Saturday afternoon, not Friday. So the tissue may sit in paraffin for 2-4 extra hours. >>> "Jackie M O'Connor" 11/17/2008 3:41 PM >>> How long are they in molten paraffin "Angela Bitting" <[EMA

RE: [Histonet] breast bx processing

We have staff in on Sunday afternoon, so we start them Friday night and let them sit in paraffin until the techs come in on Sunday. The extra paraffin has actually cut down the number of dry outs we were having. >>> "Martin, Gary" <[EMAIL PROTECTED]> 11/17/2008 2:14 PM >>> We come in and process

Re: AW: [Histonet] Fatty tissue

We've done the same. Additional time in Xylene and paraffin made a huge difference. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of

Re: [Histonet] President

not really...just want to keep this arena of scientific thinking sacred (and totally non-partisan)! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the

RE: [Histonet] Be careful, be very careful when you Vote!

I'd say we all should be more worried about Revelations >>> "Smith, Allen" <[EMAIL PROTECTED]> 11/4/2008 10:06 AM >>> Sam Histology seems to be unfamiliar with Exodus 23:1. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Sam Histology Sent: Tuesday, N

[Histonet] progressive H&E staining using Gills

We just switched our H&E stain to a progressive stain using Gill II hematoxylin. Our derm guys love it, but now our GI docs are screaming bloody murder. Does anyone have suggestions for a protocol that will make both 'relatively happy'?? Thanks for letting me pick your brains agai

Re: [Histonet] CAP Question

I sign off on all of our daily maintenance/temperature charts at the end of each month. I interpret in this question that "instrument maintenance " means the daily or monthly maintenance that we do ourselves in the lab. Such as, changing solutions on our processors and stainer

[Histonet] barcode labels vs handwritten

block. I'd appreciate your comments and suggestions about this issue. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of