ursday, February 25, 2010 9:42 AM
To: Michael Williams; Laurie Colbert
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Snap Freezing Tissue
Who supplies the 3MT Novec Engineered Fluid HFE-7100?
Thanks
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospit
...@nationwidechildrens.org]
Sent: Thursday, February 25, 2010 9:42 AM
To: Michael Williams; Laurie Colbert
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Snap Freezing Tissue
Who supplies the 3MT Novec Engineered Fluid HFE-7100?
Thanks
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children&#
u] On Behalf Of Michael
Williams
Sent: Thursday, February 25, 2010 8:55 AM
To: 'Laurie Colbert'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Snap Freezing Tissue
Hi Laurie,
We distribute Bright's Clini-RF. We hear good reports about using 3MT
NovecT
Engineered Flu
t: Re: [Histonet] Snap Freezing Tissue
To: "Andrea T. Hooper"
Cc: histonet@lists.utsouthwestern.edu, "Laurie Colbert"
, "Steve Pike"
Date: Wednesday, February 24, 2010, 10:31 PM
I learned to freeze tissue by taking a beaker and filling it with
2-methylbutane and t
Hi Laurie,
We distribute Bright's Clini-RF. We hear good reports about using 3MT NovecT
Engineered Fluid HFE-7100 which is inert and has an excellent environmental,
health and safety profile.
Regards,
Michael Williams
mjwilli...@scilogex.com
SCILOGEX, LLC
TF: (877) SCILOGEX
T: (860) 828-5614
F:
Dear Laurie,
We manufacture the Clini-RF Rapid Freezer =80 degs. C with options for a
range of freezing methods.
Best Regards
Alan Bright
Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abri...@br
Contact Jim Mullen at Hacker Instruments (800) 442-2537. He has a demo
CliniRF that I'm sure you can get at a good price.
Dorothy
Dorothy Traczyk
MTA Histology LLC
In a message dated 2/24/2010 4:55:55 P.M. Eastern Standard Time,
laurie.colb...@huntingtonhospital.com writes:
I was won
I learned to freeze tissue by taking a beaker and filling it with
2-methylbutane and then placing that beaker in a liquid nitrogen bath. You
then take place your cryomold, hold it using some long forceps, and place it
on the top of the 2-methylbutane. It will freeze the entire thing in about
10 sec
I have used the "Gayle Callis" method of freezing tissue for histological
applications for years with great success :) The tissues look fantastic.
Essentially float out a metal pan (like the ones you sterilize surgical tools
in) on a liquid nitrogen bath (in a styrofoam container or large dewa
