[ccp4bb] Workshop on Soft X-ray Crystallography at the APS, May 4, 2011
We would like to call your attention to a workshop associated with the upcoming 2011 APS Users Meeting, which will be held on May 4, 2011 at the APS, Argonne National Laboratory, USA. The title of the workshop is “Soft X-ray Crystallography”. You may find the workshop program at http://2011usersmeeting.conference.anl.gov/APSWK1Agenda.htm The web links for meeting registration and the application for a permit to enter the Argonne National Laboratory are: https://2011usersmeeting.conference.anl.gov/register http://www.aps.anl.gov/Users/Site_Access We hope you will be able to attend the APS Users Meeting and our workshop. All the best, B.C. Wang, John Rose, Gerd Rosenbaum and John Chrzas.
Re: [ccp4bb] Workshop on Soft X-ray Crystallography at the APS, May 4, 2011
Dear BC and colleagues, It is rather unclear from the title of the workshop whether it is the Crystallography that will be soft, or the X-rays. With best wishes, Gerard. -- On Fri, Apr 22, 2011 at 11:56:22AM -0400, Bi-Cheng Wang wrote: We would like to call your attention to a workshop associated with the upcoming 2011 APS Users Meeting, which will be held on May 4, 2011 at the APS, Argonne National Laboratory, USA. The title of the workshop is “Soft X-ray Crystallography”. You may find the workshop program at http://2011usersmeeting.conference.anl.gov/APSWK1Agenda.htm The web links for meeting registration and the application for a permit to enter the Argonne National Laboratory are: https://2011usersmeeting.conference.anl.gov/register http://www.aps.anl.gov/Users/Site_Access We hope you will be able to attend the APS Users Meeting and our workshop. All the best, B.C. Wang, John Rose, Gerd Rosenbaum and John Chrzas. -- === * * * Gerard Bricogne g...@globalphasing.com * * * * Global Phasing Ltd. * * Sheraton House, Castle Park Tel: +44-(0)1223-353033 * * Cambridge CB3 0AX, UK Fax: +44-(0)1223-366889 * * * ===
Re: [ccp4bb] Workshop on Soft X-ray Crystallography at the APS, May 4, 2011
I think soft crystallography uses fuzzy logic. JPK On Fri, Apr 22, 2011 at 11:08 AM, Gerard Bricogne g...@globalphasing.com wrote: Dear BC and colleagues, It is rather unclear from the title of the workshop whether it is the Crystallography that will be soft, or the X-rays. With best wishes, Gerard. -- On Fri, Apr 22, 2011 at 11:56:22AM -0400, Bi-Cheng Wang wrote: We would like to call your attention to a workshop associated with the upcoming 2011 APS Users Meeting, which will be held on May 4, 2011 at the APS, Argonne National Laboratory, USA. The title of the workshop is “Soft X-ray Crystallography”. You may find the workshop program at http://2011usersmeeting.conference.anl.gov/APSWK1Agenda.htm The web links for meeting registration and the application for a permit to enter the Argonne National Laboratory are: https://2011usersmeeting.conference.anl.gov/register http://www.aps.anl.gov/Users/Site_Access We hope you will be able to attend the APS Users Meeting and our workshop. All the best, B.C. Wang, John Rose, Gerd Rosenbaum and John Chrzas. -- === * * * Gerard Bricogne g...@globalphasing.com * * * * Global Phasing Ltd. * * Sheraton House, Castle Park Tel: +44-(0)1223-353033 * * Cambridge CB3 0AX, UK Fax: +44-(0)1223-366889 * * * === -- *** Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel: 773.608.9185 email: j-kell...@northwestern.edu ***
Re: [ccp4bb] Workshop on Soft X-ray Crystallography at the APS, May 4, 2011
I think soft crystallography uses fuzzy logic--it's a comforting alternative to the usual SAD and MAD.
Re: [ccp4bb] Workshop on Soft X-ray Crystallography at the APS, May 4, 2011
First line up 10^9 poohbears... Sent from my iPhone On 22 Apr 2011, at 19:58, Jacob Keller j-kell...@fsm.northwestern.edu wrote: I think soft crystallography uses fuzzy logic--it's a comforting alternative to the usual SAD and MAD.
Re: [ccp4bb] Using chaperones to boost expression in E. coli
Hi Michael, It worked for me with one viral enzyme but did not work with 2 others. In the successful case, I used Takara's chaperone plasmids to screen for the best composition of chaperones. The GroEL-GroES improved the yield of the soluble enzyme, but I got same activity (per 1L of media) as before. The plasmid with chaperone tig gave the biggest increase in activity (~4-8 fold). Still, the yield was rather low, in a range of 1 mg of purified protein per 1L of media. I also had to optimize the expression time and temperature to get best results. They were ~14 h at 17-20C. Interestingly, the protein failed to express in insect cells (was too poisonous for them), so the combination of a solubilizing domain, chaperones and Ecoli expression were the only available option for me. Regards Alex On Apr 22, 2011, at 8:28 AM, Kothe, Michael wrote: Dear ccp4bb, I am curious to hear of examples where the expression of well-behaved protein was achieved by the coexpression of chaperones in E. coli. I know the appropriate strains and vectors exist, but I can’t remember hearing of a successful case. I have heard anecdotally of several cases where it was tried without success (including one attempt I made myself). I also heard of concerns that the chaperones might copurify with the (now soluble) protein of interest and are difficult to remove. Thanks, Michael
[ccp4bb] Sketcher
I am attempting to create a monomer with a cis-double bond using Sketcher, but every time I tell sketcher to create the library files it makes the double bond a trans-double bond. Can anyone assist me with information on how to force it to remain cis? -- Jim Fairman, Ph D. Post-Doctoral Fellow National Institutes of Health - NIDDK The Buchanan Lab http://www-mslmb.niddk.nih.gov/buchanan/index.html Lab: 1-301-594-9229 E-mail: fairman@gmail.com james.fair...@nih.gov
Re: [ccp4bb] Workshop on Soft X-ray Crystallography at the APS, May 4, 2011
Dear Colleagues, Based on the note below from Gerard Bricogne, I would like to further clarify and highlight the intended purpose of the workshop for those of you who may be interested in attending. The planned discussions of the workshop will focus on the positive and useful benefits of using soft x-rays in macromolecular crystallography with wavelengths between 1.5 Å and 4.0 Å. The talks will also provide participants with an updated picture of the current developments around the globe, and include several key international speakers that are currently working in this fast growing field of study. I hope to see many of you at the APS, or possibly at the ACA meeting later in May or IUCr in August. Best regards, B.C. On Apr 22, 2011, at 12:08 PM, Gerard Bricogne wrote: Dear BC and colleagues, It is rather unclear from the title of the workshop whether it is the Crystallography that will be soft, or the X-rays. With best wishes, Gerard. -- On Fri, Apr 22, 2011 at 11:56:22AM -0400, Bi-Cheng Wang wrote: We would like to call your attention to a workshop associated with the upcoming 2011 APS Users Meeting, which will be held on May 4, 2011 at the APS, Argonne National Laboratory, USA. The title of the workshop is “Soft X-ray Crystallography”. You may find the workshop program at http://2011usersmeeting.conference.anl.gov/APSWK1Agenda.htm The web links for meeting registration and the application for a permit to enter the Argonne National Laboratory are: https://2011usersmeeting.conference.anl.gov/register http://www.aps.anl.gov/Users/Site_Access We hope you will be able to attend the APS Users Meeting and our workshop. All the best, B.C. Wang, John Rose, Gerd Rosenbaum and John Chrzas. -- === * * * Gerard Bricogne g...@globalphasing.com * * * * Global Phasing Ltd. * * Sheraton House, Castle Park Tel: +44-(0)1223-353033 * * Cambridge CB3 0AX, UK Fax: +44-(0)1223-366889 * * * ===
Re: [ccp4bb] Using chaperones to boost expression in E. coli
As with all these things, YMMV. In our experience, chaperone co-expression can help not at all, can produce more folded protein or can produce more soluble protein, with co-purifying chaperone. With GroEL/S at least, chaperone release is easy (add ATP) and sometimes results in the liberation of folded protein, sometimes not. One case where for us chaperones made an enabling difference: Acta Cryst. (2005). F61, 403–406 Like most things, worth trying! cheers Shaun
