Re: [ccp4bb] atomic coloring for the color blind
How about Braille for those who are blind to all colours? -- === All Things Serve the Beam === David J. Schuller modern man in a post-modern world MacCHESS, Cornell University schul...@cornell.edu
Re: [ccp4bb] atomic coloring for the color blind
David, Do you know of a program to make accurate braille representations of an electron density map :) That would be cool. Maybe in the future [See: http://www.wired.com/wiredscience/2012/08/smart-fingertips-virtual-senses/ http://www.ethlife.ethz.ch/archive_articles/100816_virtuelle_realitaet_cho/index_EN and http://www.technologyreview.com/news/428736/disney-researchers-add-virtual-touch-to-the-real-world/] For the really hard core scientist (this is a little creepy): http://io9.com/5846275/biotech-breakthrough-monkeys-can-feel-virtual-objects-using-a-brain-implant Food for thought, Steve sherron_...@yahoo.com On 6/1/2013 12:16 PM, David Schuller wrote: How about Braille for those who are blind to all colours?
[ccp4bb] anomalous scattering server down?
Is Ethan Merritt's anomalous scattering page at: http://www.bmsc.washington.edu/scatter/ down or moved, or the firewall I'm behind is blocking it? I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab
Re: [ccp4bb] anomalous scattering server down?
seems to be down sorry. you should be able to use crossec from ccp4 Jürgen On Jun 1, 2013, at 6:47 PM, Edward A. Berry wrote: Is Ethan Merritt's anomalous scattering page at: http://www.bmsc.washington.edu/scatter/ down or moved, or the firewall I'm behind is blocking it? I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
Re: [ccp4bb] anomalous scattering server down?
Hmm- looks like crosssec calculates F' and F (and cros-section). I guess you're thinking of the x-ray edge page on the same server. Ive got local copies and graphs of that in an excel spreadsheet. I was thinking about the page where you enter your protein's molecular weight, number and type of anomalous scatterers, and it tells you how accurate your data has to be to make the anomalous signal significant. Thanks, eab Bosch, Juergen wrote: seems to be down sorry. you should be able to use crossec from ccp4 Jürgen On Jun 1, 2013, at 6:47 PM, Edward A. Berry wrote: Is Ethan Merritt's anomalous scattering page at: http://www.bmsc.washington.edu/scatter/ down or moved, or the firewall I'm behind is blocking it? I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
Re: [ccp4bb] anomalous scattering server down?
Bernhard Rupp has a calculator of Bijvoet ratios: http://www.ruppweb.org/new_comp/anomalous_scattering.htm Blaine Mooers Assistant Professor Director Macromolecular Crystallography Lab Member Stephenson Cancer Center Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center Rm. 466 Letter address: P.O. Box 26901, BRC 466 Oklahoma City, OK 73190 Shipping address: 975 NE 10th Street, BRC 466 Oklahoma City, OK 73104-5419 office: (405) 271-8300 lab: (405) 271-8313 fax: (405) 271-3910 e-mail: blaine-moo...@ouhsc.edu Faculty webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/faculty/blaine-mooers-ph-d- X-ray lab webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/department-facilities/macromolecular-crystallography-laboratory SAXS Links webpage: http://www.oumedicine.com/docs/default-source/ad-biochemistry-workfiles/small-angle-x-ray-scattering-links.html?sfvrsn=0 From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Edward A. Berry [ber...@upstate.edu] Sent: Saturday, June 01, 2013 6:09 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] anomalous scattering server down? Hmm- looks like crosssec calculates F' and F (and cros-section). I guess you're thinking of the x-ray edge page on the same server. Ive got local copies and graphs of that in an excel spreadsheet. I was thinking about the page where you enter your protein's molecular weight, number and type of anomalous scatterers, and it tells you how accurate your data has to be to make the anomalous signal significant. Thanks, eab Bosch, Juergen wrote: seems to be down sorry. you should be able to use crossec from ccp4 Jürgen On Jun 1, 2013, at 6:47 PM, Edward A. Berry wrote: Is Ethan Merritt's anomalous scattering page at: https://urldefense.proofpoint.com/v1/url?u=http://www.bmsc.washington.edu/scatter/k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0Ar=ftLbjJYpc5s5JQz9Q6qd7uT7FxPLb4V0aIwH4RJhyZU%3D%0Am=vriAf%2FYLAE3OBEruPk8rCrKni5F1ZSfZwaGrrX0lwuk%3D%0As=57fb37f57cec12f6c778c53c6bfbdd04afc795f050605681dca25305998c6af0 down or moved, or the firewall I'm behind is blocking it? I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 https://urldefense.proofpoint.com/v1/url?u=http://lupo.jhsph.edu/k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0Ar=ftLbjJYpc5s5JQz9Q6qd7uT7FxPLb4V0aIwH4RJhyZU%3D%0Am=vriAf%2FYLAE3OBEruPk8rCrKni5F1ZSfZwaGrrX0lwuk%3D%0As=d80576bcb5bffce1fe3f7ffe04c9ec7a4b3338c452e984d4efcbd8036ce5b83c
Re: [ccp4bb] anomalous scattering server down?
That's what I wanted. Results are not promising- Bijvoet differences would be less than 2% at peak, and this is not lysozyme. Thanks, all, eab Mooers, Blaine H.M. (HSC) wrote: Bernhard Rupp has a calculator of Bijvoet ratios: http://www.ruppweb.org/new_comp/anomalous_scattering.htm Blaine Mooers Assistant Professor Director Macromolecular Crystallography Lab Member Stephenson Cancer Center Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center Rm. 466 Letter address: P.O. Box 26901, BRC 466 Oklahoma City, OK 73190 Shipping address: 975 NE 10th Street, BRC 466 Oklahoma City, OK 73104-5419 office: (405) 271-8300 lab: (405) 271-8313 fax: (405) 271-3910 e-mail: blaine-moo...@ouhsc.edu Faculty webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/faculty/blaine-mooers-ph-d- X-ray lab webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/department-facilities/macromolecular-crystallography-laboratory SAXS Links webpage: http://www.oumedicine.com/docs/default-source/ad-biochemistry-workfiles/small-angle-x-ray-scattering-links.html?sfvrsn=0 From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Edward A. Berry [ber...@upstate.edu] Sent: Saturday, June 01, 2013 6:09 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] anomalous scattering server down? Hmm- looks like crosssec calculates F' and F (and cros-section). I guess you're thinking of the x-ray edge page on the same server. Ive got local copies and graphs of that in an excel spreadsheet. I was thinking about the page where you enter your protein's molecular weight, number and type of anomalous scatterers, and it tells you how accurate your data has to be to make the anomalous signal significant. Thanks, eab Bosch, Juergen wrote: seems to be down sorry. you should be able to use crossec from ccp4 Jürgen On Jun 1, 2013, at 6:47 PM, Edward A. Berry wrote: Is Ethan Merritt's anomalous scattering page at: https://urldefense.proofpoint.com/v1/url?u=http://www.bmsc.washington.edu/scatter/k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0Ar=ftLbjJYpc5s5JQz9Q6qd7uT7FxPLb4V0aIwH4RJhyZU%3D%0Am=vriAf%2FYLAE3OBEruPk8rCrKni5F1ZSfZwaGrrX0lwuk%3D%0As=57fb37f57cec12f6c778c53c6bfbdd04afc795f050605681dca25305998c6af0 down or moved, or the firewall I'm behind is blocking it? I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 https://urldefense.proofpoint.com/v1/url?u=http://lupo.jhsph.edu/k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0Ar=ftLbjJYpc5s5JQz9Q6qd7uT7FxPLb4V0aIwH4RJhyZU%3D%0Am=vriAf%2FYLAE3OBEruPk8rCrKni5F1ZSfZwaGrrX0lwuk%3D%0As=d80576bcb5bffce1fe3f7ffe04c9ec7a4b3338c452e984d4efcbd8036ce5b83c
Re: [ccp4bb] anomalous scattering server down?
How does that compare to something that readily works with Fe, such as horse hemoglobin on a home lab copper X-ray system with 2 Fe in 291 residues in the asymmetric unit? From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Edward A. Berry [ber...@upstate.edu] Sent: Saturday, June 01, 2013 6:46 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] anomalous scattering server down? That's what I wanted. Results are not promising- Bijvoet differences would be less than 2% at peak, and this is not lysozyme. Thanks, all, eab Mooers, Blaine H.M. (HSC) wrote: Bernhard Rupp has a calculator of Bijvoet ratios: http://www.ruppweb.org/new_comp/anomalous_scattering.htm Blaine Mooers Assistant Professor Director Macromolecular Crystallography Lab Member Stephenson Cancer Center Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center Rm. 466 Letter address: P.O. Box 26901, BRC 466 Oklahoma City, OK 73190 Shipping address: 975 NE 10th Street, BRC 466 Oklahoma City, OK 73104-5419 office: (405) 271-8300 lab: (405) 271-8313 fax: (405) 271-3910 e-mail: blaine-moo...@ouhsc.edu Faculty webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/faculty/blaine-mooers-ph-d- X-ray lab webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/department-facilities/macromolecular-crystallography-laboratory SAXS Links webpage: http://www.oumedicine.com/docs/default-source/ad-biochemistry-workfiles/small-angle-x-ray-scattering-links.html?sfvrsn=0 From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Edward A. Berry [ber...@upstate.edu] Sent: Saturday, June 01, 2013 6:09 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] anomalous scattering server down? Hmm- looks like crosssec calculates F' and F (and cros-section). I guess you're thinking of the x-ray edge page on the same server. Ive got local copies and graphs of that in an excel spreadsheet. I was thinking about the page where you enter your protein's molecular weight, number and type of anomalous scatterers, and it tells you how accurate your data has to be to make the anomalous signal significant. Thanks, eab Bosch, Juergen wrote: seems to be down sorry. you should be able to use crossec from ccp4 Jürgen On Jun 1, 2013, at 6:47 PM, Edward A. Berry wrote: Is Ethan Merritt's anomalous scattering page at: https://urldefense.proofpoint.com/v1/url?u=http://www.bmsc.washington.edu/scatter/k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0Ar=ftLbjJYpc5s5JQz9Q6qd7uT7FxPLb4V0aIwH4RJhyZU%3D%0Am=vriAf%2FYLAE3OBEruPk8rCrKni5F1ZSfZwaGrrX0lwuk%3D%0As=57fb37f57cec12f6c778c53c6bfbdd04afc795f050605681dca25305998c6af0 down or moved, or the firewall I'm behind is blocking it? I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 https://urldefense.proofpoint.com/v1/url?u=http://lupo.jhsph.edu/k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0Ar=ftLbjJYpc5s5JQz9Q6qd7uT7FxPLb4V0aIwH4RJhyZU%3D%0Am=vriAf%2FYLAE3OBEruPk8rCrKni5F1ZSfZwaGrrX0lwuk%3D%0As=d80576bcb5bffce1fe3f7ffe04c9ec7a4b3338c452e984d4efcbd8036ce5b83c
Re: [ccp4bb] anomalous scattering server down?
Maybe I was a bit hasty- the Hb example gives only 2.4% difference; 2.0 at CuKa, if I'm interpreting the output correctly. Maybe in the F. transform the random noise averages out and the signal rises above. But the Hb xtal probably diffracts really well with R-merge 3 or 4 in the low resolution shell. This is a huge multisubunit membrane protein complex, such as I'm happy with overall rmerge 12% and 6-8% in the low resolution shell. But if I don't say that in the grant proposal . . . Anyway this is all hypothetical now- the protein still needs to be expressed, purified, crystallized. Thanks, eab Jim Pflugrath wrote: How does that compare to something that readily works with Fe, such as horse hemoglobin on a home lab copper X-ray system with 2 Fe in 291 residues in the asymmetric unit? From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Edward A. Berry [ber...@upstate.edu] Sent: Saturday, June 01, 2013 6:46 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] anomalous scattering server down? That's what I wanted. Results are not promising- Bijvoet differences would be less than 2% at peak, and this is not lysozyme. Thanks, all, eab Mooers, Blaine H.M. (HSC) wrote: Bernhard Rupp has a calculator of Bijvoet ratios: http://www.ruppweb.org/new_comp/anomalous_scattering.htm Blaine Mooers Assistant Professor Director Macromolecular Crystallography Lab Member Stephenson Cancer Center Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center Rm. 466 Letter address: P.O. Box 26901, BRC 466 Oklahoma City, OK 73190 Shipping address: 975 NE 10th Street, BRC 466 Oklahoma City, OK 73104-5419 office: (405) 271-8300 lab: (405) 271-8313 fax: (405) 271-3910 e-mail: blaine-moo...@ouhsc.edu Faculty webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/faculty/blaine-mooers-ph-d- X-ray lab webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/department-facilities/macromolecular-crystallography-laboratory SAXS Links webpage: http://www.oumedicine.com/docs/default-source/ad-biochemistry-workfiles/small-angle-x-ray-scattering-links.html?sfvrsn=0 From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Edward A. Berry [ber...@upstate.edu] Sent: Saturday, June 01, 2013 6:09 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] anomalous scattering server down? Hmm- looks like crosssec calculates F' and F (and cros-section). I guess you're thinking of the x-ray edge page on the same server. Ive got local copies and graphs of that in an excel spreadsheet. I was thinking about the page where you enter your protein's molecular weight, number and type of anomalous scatterers, and it tells you how accurate your data has to be to make the anomalous signal significant. Thanks, eab Bosch, Juergen wrote: seems to be down sorry. you should be able to use crossec from ccp4 Jürgen On Jun 1, 2013, at 6:47 PM, Edward A. Berry wrote: Is Ethan Merritt's anomalous scattering page at: https://urldefense.proofpoint.com/v1/url?u=http://www.bmsc.washington.edu/scatter/k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0Ar=ftLbjJYpc5s5JQz9Q6qd7uT7FxPLb4V0aIwH4RJhyZU%3D%0Am=vriAf%2FYLAE3OBEruPk8rCrKni5F1ZSfZwaGrrX0lwuk%3D%0As=57fb37f57cec12f6c778c53c6bfbdd04afc795f050605681dca25305998c6af0 down or moved, or the firewall I'm behind is blocking it? I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 https://urldefense.proofpoint.com/v1/url?u=http://lupo.jhsph.edu/k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0Ar=ftLbjJYpc5s5JQz9Q6qd7uT7FxPLb4V0aIwH4RJhyZU%3D%0Am=vriAf%2FYLAE3OBEruPk8rCrKni5F1ZSfZwaGrrX0lwuk%3D%0As=d80576bcb5bffce1fe3f7ffe04c9ec7a4b3338c452e984d4efcbd8036ce5b83c
Re: [ccp4bb] anomalous scattering server down?
On Saturday, 01 June 2013, Edward A. Berry wrote: Is Ethan Merritt's anomalous scattering page at: http://www.bmsc.washington.edu/scatter/ down or moved, or the firewall I'm behind is blocking it? The UW, in its infinite wisdom, scheduled a power outage today so that they could replace the network infrastructure. Then to be on the safe side someone took down the network in my building early. Anyhow, when I get the all-clear I will head back into the lab to restart everything. Ethan I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab
Re: [ccp4bb] anomalous scattering server down?
I put together a little jiffy for feasibility of MAD experiments here: http://bl831.als.lbl.gov/xtalsize.html It does not calculate the f value for you, but in general f is ~4 electrons for K edges and ~10 electrons for L edges. Crossec can give you more accurate values than this as long as you are far away from the absorption edge (10-100 eV), but if you are right on top of the white line, then you have to measure f anyway using an absorption scan. That said, it is very rare for the white line to be more than double the edge jump and Fe is not particularly spectacular for white lines, so I wouldn't expect much more than ~6 electrons from iron. -James Holton MAD Scientist On 6/2/2013 8:47 AM, Edward A. Berry wrote: Is Ethan Merritt's anomalous scattering page at: http://www.bmsc.washington.edu/scatter/ down or moved, or the firewall I'm behind is blocking it? I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab
Re: [ccp4bb] anomalous scattering server down?
Similar result. Taking all defaults except #atoms, amt protein: kDa #res #sites BjvtRatio protein of interest: LBL: 469 12 0.0153 (4 e-) Ruppweb 427312 0.016(edge + 1 ev) Ruppweb 427312 0.013(CuKa) Hemoglobin protomer LBL 32 2 0.0239 (4 e-) Ruppweb 291 2 0.024(edge + 1 ev) Ruppweb 291 2 0.020(CuKa) James Holton wrote: I put together a little jiffy for feasibility of MAD experiments here: http://bl831.als.lbl.gov/xtalsize.html It does not calculate the f value for you, but in general f is ~4 electrons for K edges and ~10 electrons for L edges. Crossec can give you more accurate values than this as long as you are far away from the absorption edge (10-100 eV), but if you are right on top of the white line, then you have to measure f anyway using an absorption scan. That said, it is very rare for the white line to be more than double the edge jump and Fe is not particularly spectacular for white lines, so I wouldn't expect much more than ~6 electrons from iron. -James Holton MAD Scientist On 6/2/2013 8:47 AM, Edward A. Berry wrote: Is Ethan Merritt's anomalous scattering page at: http://www.bmsc.washington.edu/scatter/ down or moved, or the firewall I'm behind is blocking it? I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab
Re: [ccp4bb] anomalous scattering server down?
Not surprising since I'm sure they are both based on the Crick-Magdoff equation (http://dx.doi.org/10.1107/S0365110X56002552) or the very similar Hendrickson-Teeter equation (http://dx.doi.org/10.1038/290107a0). Practically important thing is the signal-to-noise ratio. Roughly speaking, to measure a difference of 1.5% you need a signal-to-noise that is at least 1/1.5% = 60. So, if you typically get I/sd ~ 10 (cumulative to the resolution of interest, wich is usually ~3.5A for SAD phasing), then you need to average over (60/10)^2 = 36 crystals to bump the overall I/sd up to what you need. This is assuming the crystals are all isomorphous of course. With a 3% Bijvoet ratio, you only need I/sd ~ 30. This is not all that hard to do with a single good-quality crystal at normal multiplicity (~3-4). I think this is why most successful MAD/SAD structure determinations have Bijvoet ratios around 3%. The world record (http://dx.doi.org/10.1107/S0907444906038534) used a Bijvoet ratio of 0.5% and very high multiplicity. Kay wrote a paper about this asymptotic I/sd (http://dx.doi.org/10.1107/S0907444910014836), which tops out around 30 for most diffractometers. The source of this limitation is actually detector calibration. That hasn't exactly been published yet, except in the detector literature of course (http://dx.doi.org/10.1063/1.1488674 section IV.B), but who has time to read that? -James Holton MAD Scientist On 6/2/2013 12:43 PM, Edward A. Berry wrote: Similar result. Taking all defaults except #atoms, amt protein: kDa #res #sites BjvtRatio protein of interest: LBL: 469 12 0.0153 (4 e-) Ruppweb 427312 0.016(edge + 1 ev) Ruppweb 427312 0.013(CuKa) Hemoglobin protomer LBL 32 2 0.0239 (4 e-) Ruppweb 291 2 0.024(edge + 1 ev) Ruppweb 291 2 0.020(CuKa) James Holton wrote: I put together a little jiffy for feasibility of MAD experiments here: http://bl831.als.lbl.gov/xtalsize.html It does not calculate the f value for you, but in general f is ~4 electrons for K edges and ~10 electrons for L edges. Crossec can give you more accurate values than this as long as you are far away from the absorption edge (10-100 eV), but if you are right on top of the white line, then you have to measure f anyway using an absorption scan. That said, it is very rare for the white line to be more than double the edge jump and Fe is not particularly spectacular for white lines, so I wouldn't expect much more than ~6 electrons from iron. -James Holton MAD Scientist On 6/2/2013 8:47 AM, Edward A. Berry wrote: Is Ethan Merritt's anomalous scattering page at: http://www.bmsc.washington.edu/scatter/ down or moved, or the firewall I'm behind is blocking it? I want to check feasibility of a native-iron MAD experiment, and I'm not very good at math. thanks, eab
