Re: [ccp4bb] Crystallization Screens
Dear FBDD Evangelist, As Andreas mentioned, Emerald is now Rigaku Reagents. We still offer this screen, but under different name (Wizard pH Buffer screen). Here’s a link: http://www.rigakureagents.com/p-11-wizard-ph-buffer-96-well-block-plate.aspx Best, Angela From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hernani Silvestre Sent: Monday, March 06, 2017 3:56 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Crystallization Screens Dear community, Quite likely you have been in this situation, you come across a screen where you have hits but the screen has been discontinued. I am looking for the following screen: Emerald Biosystems pHAT buffer screen. I did find a pdf with some of the buffers described (but , as usual, the well composition I am interested I cant see it there..the pdf is incomplete. Does anyone know the 96-well composition of this buffer screen? Not sure what happened to Emerald Bio, they are no longer active (oh dear...) Many thanks FBDD Evangelist
[ccp4bb] “Femtosecond crystallography of membrane proteins in lipidic cubic phase (LCP)” Webinar
Dear colleagues, I would like to draw your attention to an upcoming free, educational webinar to be presented by Dr. Wei Liu titled “Femtosecond crystallography of membrane proteins in lipidic cubic phase (LCP).” Despite recent technological advances in heterologous expression, stabilization and crystallization of membrane proteins, structural studies remain difficult. Wei’s presentation will describe the use of serial femtosecond crystallography of membrane proteins in LCP and how this approach holds promise to interrogate this difficult, yet important, class of proteins. This webinar is scheduled to occur on Thursday, November 13th at 11:00 AM CST (9:00 AM PST / 5:00 PM GMT). You can find an abstract and registration link for this webinar at: http://www.rigaku.com/webinars. We hope you will join us for the event. Best regards, Angela Criswell -- Angela Criswell VP, Life Science X-ray Products Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 USA Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.commailto:angela.crisw...@rigaku.com URL: http://www.rigaku.com Subscribe to Rigaku eNewslettershttp://www.rigaku.com/subscribe
[ccp4bb] Radiation Damage in Macromolecular Crystallography: What is it and why do we care? Webinar
Dear colleagues, I would like to draw your attention to an upcoming free, educational webinar to be presented by Elspeth Garman, Ph. D. titled Radiation Damage in Macromolecular Crystallography: What is it and why do we care? Radiation damage inflicted during diffraction data collection in macromolecular crystallography has re-emerged in the last decade as a major experimental and computational challenge. Even for crystals held at 100 K such damage can result in severe data quality degradation and the appearance of artifacts in solved structures which affect biological interpretations. In this webinar, the observable symptoms and basic physical processes involved in radiation damage will be described and the concept of absorbed dose as the basic metric against which to monitor the experimentally observed changes will be outlined. Investigations into radiation damage in macromolecular crystallography are ongoing and the number of studies is rapidly increasing. Our understanding is improving and various possible counter measures will be summarized. This webinar is scheduled to occur on Thursday, October 4th at 10:00 AM CDT (8:00 AM PDT / 4:00 PM GMT). You can find more information, including a registration link at: http://www.rigaku.com/webinars. This page also includes links to recordings of past Rigaku webinars. Best regards, Angela Criswell NOTE: This webinar is part of a free webinar series designed to provide educational and training materials in X-ray crystallography methods. Each webinar will address a new topic ranging from optimization of crystallization conditions to solving the phase problem. You can find links to recordings and slides from past webinars at http://www.rigaku.com/webinars. We also invite anyone to submit topic suggestions for future webinars and hope that you'll provide feedback for our previous webinars. -- Angela R. Criswell, Ph. D. VP, Life Science X-ray Products Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 USA Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.rigaku.com
[ccp4bb] Web Seminar: Home Lab SAD phasing with HKL-3000: From data collection to refined models in less than an hour
Dear colleagues, I would like to draw your attention to an upcoming free, educational webinar to be presented by Jim Pflugrath, Ph. D. titled Home Lab SAD phasing with HKL-3000: From data collection to refined models in less than an hour. This interactive tutorial and webinar will demonstrate how to use HKL-3000 to process diffraction images, find the anomalous substructure with SHELXD, phase with SHELXC, MLPHARE, and DM, then build with ARP/wARP and refine with REFMAC (Kudos to all the authors of these programs!). Emphasis will be on practical tips and how to interpret the output. Relatively low redundancy diffraction datasets will be used as examples to dispel some of the myths about sulfur and selenium Home Lab SAD phasing. This webinar is scheduled to occur on Thursday, November 17th 10:00 AM CST (8:00 AM PST / 4:00 PM GMT). You can find more information, including a registration link at: http://www.rigaku.com/protein/webinars.html. Best regards, Angela NOTE: You can watch some of our past webinars at: http://www.rigaku.com/protein/webinars-past.html. This list of webinars includes educational topics, such as data processing with d*TREK, mosflm, XDS and HKL as well as topics on diffraction data collection. Also, don't miss the great talks and historical perspectives from industry experts such as Michael Rossmann, Brian Matthews and Ian Wilson. -- Angela R. Criswell, Ph. D. Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 USA Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.rigaku.com
[ccp4bb] Web Seminar: An Insider's Guide to CrystalClear and d*TREK: Improve Your Results with these New Features and Tips
Dear colleagues, I would like to draw your attention to an upcoming free, educational webinar to be presented by Jim Pflugrath, Ph. D. titled An Insider's Guide to CrystalClear and d*TREK: Improve Your Results with these New Features and Tips. In this webinar, Jim will discuss three important new features or tips for using CrystalClear and d*TREK which will lead to better results: lower Rmeas and higher I/sigmaI. New input parameters for finding spots is one new feature. Another feature is a better way perform positional refinement before and during Bragg reflection integration. A third tip is how to improve your scaling results by changing the input parameters to dtscaleaverage. These tips and others will give you better results with CrystalClear. This webinar is scheduled to occur on Thursday, September 1st 10:00 AM CDT (8:00 AM PDT / 4:00 PM GMT). You can find more information, including a registration link at: http://www.rigaku.com/protein/webinars.html. Best regards, Angela Criswell -- Angela R. Criswell, Ph. D. VP, Life Sciences Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 USA Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.rigaku.com
[ccp4bb] Position available with Rigaku - Application Scientist in Life Sciences Division - The Woodlands, TX
Rigaku has an immediate opening for a motivated application scientist to join the Life Sciences group in our North American headquarters, located in The Woodlands, TX. The candidate must have a PhD degree in a relevant field and have previous experience in structural biology X-ray methodologies such as protein crystallography and small angle X-ray scattering of macromolecules. Candidates must have excellent oral and written communication skills, must be able to manage projects and deadlines effectively, and should possess both a strong customer service ethic and strong technical troubleshooting skills. Additionally, candidates must be prepared and able to travel without restriction to X-ray facilities throughout North America and internationally. Fluent English is required but does not need to be your first language. Preference will be given to scientists with hands-on experience in the use and maintenance of X-ray systems, experience with teaching and a high level of aptitude in X-ray diffraction and scattering methods. Since its inception in Japan in 1951, Rigaku has been at the forefront of analytical and industrial instrumentation technology. With hundreds of major innovations to its credit, Rigaku and its subsidiary companies are world leaders in the fields of protein X-ray crystallography and scattering, automation, cryogenics and X-ray optics. Rigaku offers a rewarding work environment and excellent benefits. To apply, please send a curriculum vitae with cover letter and three potential referees. Please include job reference: LSAPP011 and send items by email to resu...@rigaku.com, by fax to +1 281-364-3628 or by mail to Rigaku Americas, 9009 New Trails Dr., The Woodlands, TX 77381-5209, Attention: Human Resources. All applicants are kept in strict confidence. Benefits package including Health, Dental, Vision, STD, LTD, Life insurance, section 125 flexible spending accounts, a 401(k) plan and more. Rigaku is an EEO/AA Employer M/F/D/V. Applicants must be able to prove they can legally work in the US. -- Angela R. Criswell, Ph. D. VP, Life Sciences Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 USA Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.rigaku.com
[ccp4bb] Small Angle X-ray Scattering (SAXS) Techniques for Macromolecules Webinar
Dear colleagues, I would like to draw your attention to an upcoming free, educational webinar to be presented by Angela Criswell, Ph. D. titled Small Angle X-ray Scattering (SAXS) Techniques for Macromolecules. Small angle X-ray scattering is a technique used to study dilute solutions of macromolecules. SAXS measurements can give valuable information about the low resolution structural characteristics and in some cases a model of the protein shape. Specifically, SAXS is particularly useful for giving immediate feedback whether your protein is monodispersed or aggregated and whether your protein is folded or unfolded in solution. In addition, several examples in the literature demonstrate the use of SAXS to identify the correct multimeric states or proteins, thus eliminating the ambiguity associated with crystal-packing induced oligomers. This webinar will summarize various applications of SAXS for macromolecules and explore some practical considerations for collecting and processing SAXS data. This webinar is scheduled to occur on Thursday, June 23rd 10:00 AM CDT (8:00 AM PDT / 4:00 PM GMT). You can find more information, including a registration link at: http://www.rigaku.com/protein/webinars.html. Best regards, Angela Criswell -- Angela R. Criswell, Ph. D. VP, Life Sciences Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 USA Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.rigaku.com
[ccp4bb] 'SAD Phasing using Iodide Ions' Webinar
Dear colleagues, I would like to draw your attention to an upcoming free, educational webinar to be presented by Tom Edwards, Ph.D. titled SAD phasing at rotating anode wavelengths using iodide ions. In this webinar, Tom will present a general overview of using of iodide ion soaks and (SAD) experiments for de novo phase determination. They've used this technique across a wide range of crystallization conditions with successful structure determination in at least twenty cases. I hope you'll join us to learn more. This webinar is scheduled to occur on Thursday, May 12th at 12:00 PM CDT (10:00 AM PDT / 6:00 PM GMT). You can find more information, including a registration link at: http://www.rigaku.com/protein/webinars.html Best regards, Angela -- Angela R. Criswell, Ph. D. Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 USA Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.rigaku.com
[ccp4bb] Webinar: Brian Matthews presents Protein Crystallography: Getting in on the Ground Floor
Dear colleagues, I would like to draw your attention to an upcoming webinar to be presented by Brian Matthews, Ph.D. titled Protein Crystallography: Getting in on the Ground Floor. Brian Matthews received both his B.S. (1959) and Ph.D. (1964) from the University of Adelaide, completing his studies during a pivotal period in X-ray crystallography—at around the same time that the first three-dimensional structure of a protein, myoglobin, was determined. I had the good fortune to be a postdoc at the MRC Lab in Cambridge and to participate in the chymotrypsin structure determination in David Blow's group. In this webinar, Brian will share a personal recollection of the structural biology at that time. This webinar is scheduled to occur on Wednesday, February 23rd at 12:00 PM CST (10:00 AM PST / 6:00 PM GMT). You can find more information, including a registration link at: http://www.rigaku.com/protein/webinars.html Best regards, Angela -- Angela R. Criswell, Ph. D. Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 USA Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.rigaku.com
[ccp4bb] Protein Crystallization Webinar
Dear colleagues, I would like to draw your attention to an upcoming webinar to be presented by Janet Newman, Ph.D. titled When you need a challenge, try protein crystallisation: Tips and suggestions for helping you over the hurdles. Janet currently runs theBio21 Collaborative Crystallisation Centre in Melbourne, Australia. In this webinar, she will provide insights gleaned from more than 20 years of experience regarding one of the most common bottlenecks in protein crystallography. This webinar is scheduled to occur on Thursday, November 18th at 4:00 PM CDT (2:00 PM PDT / 10:00 PM GMT). You can find more information, including a registration link at: http://www.rigaku.com/protein/webinars.html Best regards, Angela -- Angela R. Criswell, Ph.D. Rigaku Americas Corp angela.crisw...@rigaku.com
[ccp4bb] Webinar -- Scientific inquiry, inference and critical reasoning in the macromolecular crystallography curriculum
Dear colleagues, I would like to draw your attention to an upcoming webinar to be presented by Bernhard Rupp titled Scientific inquiry, inference and critical reasoning in the macromolecular crystallography curriculum. In this webinar, Bernhard will expand on his recent Journal of Applied Crystallography article that discusses higher education curricula in the context of scientific analysis. Bernhard analyzes recent cases of high profile structure retractions and argues that With the great power of modern crystallography comes great responsibility for its appropriate use. This webinar is scheduled to occur on Thursday, October 21st at 10:00 AM PDT (13:00 EDT / 18:00 GMT+1). You can find more information, including a registration link at: http://www.rigaku.com/protein/webinars.html Best regards, Angela -- Angela R. Criswell, Ph.D. Rigaku Americas Corp angela.crisw...@rigaku.com
[ccp4bb] Phenix Structure Solution Refinement Webinar
Dear colleagues, I would like to draw your attention to an upcoming educational webinar to be presented by Paul Adams titled Structure Solution and Refinement with Phenix which is scheduled to occur Thursday, August 26th at 10:00 PDT (13:00 PM EST /17:00 UTC/GMT). You can find more information including a registration link at the following site: http://www.rigaku.com/protein/webinars.html Best regards, Angela
[ccp4bb] d*TREK Data Processing Webinar
Dear colleagues, I would like to draw your attention to an upcoming educational webinar that will cover data processing with d*TREK. This webinar is the 4th in the previously posted webinar series to cover X-ray diffraction data processing packages. Next week's webinar, presented by Jim Pflugrath, is titled Diffraction Image Processing with d*TREK and will take place on April 22nd, 2010 at 10:00 AM EST (14:00 GMT). You can find more information including a registration link at the following site: http://www.rigaku.com/protein/webinars.html Best regards, Angela -- Angela R. Criswell, Ph. D. VP, Life Sciences Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.Rigaku.com
[ccp4bb] mosflm Data Processing Webinar
Dear colleagues, I would like to draw your attention to an upcoming educational webinar that will cover data processing with mosflm. This webinar is the 3rd in the previously posted webinar series to cover X-ray diffraction data processing packages. This week's webinar, presented by Andrew Leslie, is titled Processing diffraction data with imosflm and will take place on March 18th, 2010 at 11:00 AM EST (3:00 GMT). You can find more information including a registration link at the following site: http://www.rigaku.com/protein/webinars.html Best regards, Angela -- Angela R. Criswell, Ph. D. VP, Life Sciences Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.Rigaku.com
[ccp4bb] XDS Data Processing webinar
Dear colleagues, I would like to draw your attention to an upcoming educational webinar that will cover data processing with XDS. This webinar is the 2nd in the previously posted webinar series to cover X-ray diffraction data processing packages. This week's webinar, presented by Kay Diederichs, is titled Practical Approaches to Data Processing with XDS and will take place on February 25, 2010 at 9:00 AM EST (2:00 GMT). You can find more information including a registration link at the following site: http://www.rigaku.com/protein/webinars.html Best regards, Angela -- Angela R. Criswell, Ph. D. VP, Life Sciences Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.Rigaku.com
[ccp4bb] Data processing webinars
I would like to draw your attention to an upcoming free webinar series that will cover several of the most widely used X-ray diffraction data processing packages. This series is part of a larger webinar program that focuses on topics 'near and dear' to the crystallographic community. The first webinar in this series will take place on January 21st, 2010 at 9:00 AM EST and will cover HKL. You can find more information and register for these webinars at the following site: http://www.rigaku.com/protein/webinars.html. We plan to cover a different software package each month from now until April or May, based on the availability of the various software authors and experts. The primary goal for this series is to teach users how to use the different data processing packages and interpret results. As we receive additional date and time commitments we will update the above web page to include additional registration links. Best regards, Angela -- Angela R. Criswell, Ph. D. Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.Rigaku.com
Re: [ccp4bb] Data processing webinars - correction
Please note a correction in the time zone. The correct time for the webinar is 9:00 AM CST so that would be 10:00 AM EST (15:00 UTC/GMT). Angela Angela Criswell angela.crisw...@rigaku.com Sent by: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK 01/20/2010 05:29 PM Please respond to Angela Criswell angela.crisw...@rigaku.com To CCP4BB@JISCMAIL.AC.UK cc Subject [ccp4bb] Data processing webinars I would like to draw your attention to an upcoming free webinar series that will cover several of the most widely used X-ray diffraction data processing packages. This series is part of a larger webinar program that focuses on topics 'near and dear' to the crystallographic community. The first webinar in this series will take place on January 21st, 2010 at 9:00 AM EST and will cover HKL. You can find more information and register for these webinars at the following site: http://www.rigaku.com/protein/webinars.html. We plan to cover a different software package each month from now until April or May, based on the availability of the various software authors and experts. The primary goal for this series is to teach users how to use the different data processing packages and interpret results. As we receive additional date and time commitments we will update the above web page to include additional registration links. Best regards, Angela -- Angela R. Criswell, Ph. D. Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.Rigaku.com
[ccp4bb] Position available with Rigaku - Application Scientist in Life Sciences Division - The Woodlands, TX USA
Rigaku has an immediate opening for a motivated application scientist to join the Life Sciences group in our North American headquarters, located in The Woodlands, TX. Since its inception in Japan in 1951, Rigaku has been at the forefront of analytical and industrial instrumentation technology. With hundreds of major innovations to its credit, Rigaku and its subsidiary companies are world leaders in the fields of protein X-ray crystallography, automation, cryogenics and X-ray optics. Rigaku offers a rewarding work environment and excellent benefits. The successful candidate will join the world?s leading provider of analytical X-ray equipment, assist in development and implementation of software improvements, and lead software and hardware demonstration/training sessions in support of both pre-sales and post-sales activities. The candidate must have a PhD degree in a relevant field and have previous experience in protein crystallography. Candidates must have excellent oral and written communication skills, must be able to manage projects and deadlines effectively and should possess both a strong customer service ethic and strong technical troubleshooting skills. Additionally, candidates must be prepared and able to travel without restriction to X-ray facilities throughout North America, with possibility for international travel. Fluent English is required but does not need to be your first language. Preference will be given to scientists with hands-on experience in the use and maintenance of X-ray systems, familiarity with high-throughput automated systems, experience with teaching and a high level of aptitude in X-ray instrumentation and diffraction methods. Please send resumes by mail to Rigaku Americas, 9009 New Trails Dr., The Woodlands, TX 77381-5209, Attention: Human Resources, by fax to +1 281-364-3628 or by email to resu...@rigaku.com. Rigaku offers a rewarding work environment and excellent benefits. All applicants are kept in strict confidence. Benefits package including Health, Dental, Vision, STD, LTD, Life insurance, section 125 flexible spending accounts, a 401(k) plan and more. Rigaku is an EEO/AA Employer M/F/D/V. Applicants must be able to prove they can legally work in the US. For more information, please visit the job listing at http://www.rigaku.com/jobs.html#app-sci -- Angela R. Criswell, Ph. D. VP, Life Sciences Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 Ph: +1 281 362 2300 ext. 216 Fax: +1 281 364 3628 Email: angela.crisw...@rigaku.com URL: http://www.Rigaku.com
Re: [ccp4bb] protein purity in SAXS
Dear Li, The SIBYLS Beamline web page has useful sample prep information at http://bl1231.als.lbl.gov/saxs_protocols/index.php. They also have a link to a very good SAXS review article. Cheers, Angela Li Sheng biol...@gmail.com Sent by: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK 04/23/2009 06:19 AM Please respond to Li Sheng biol...@gmail.com To CCP4BB@JISCMAIL.AC.UK cc Subject [ccp4bb] protein purity in SAXS Dear all, Is there anyone with experience in small angle x-ray scattering? Could anyone please tell me the requirements for protein sample purity and concentration? Thanx in advance. Sincerely, Li __ Email:s...@sibs.ac.cn Institute of Biochemistry and Cell Biology Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue-Yang Road, Shanghai 200031, China Tel: +86-21-5492-1217 __
[ccp4bb] Application Scientist position available
Dear all, Rigaku, the world's largest analytical X-ray company, is seeking an application scientist to work with our ACTOR team. ACTOR, Automated Crystal Transport, Orientation and Retrieval, is the most popular automated sample changer for X-ray crystallography. You will help install, maintain, train and support ACTOR users worldwide. You will also develop new features and help support internal users of the robot and other advanced projects at Rigaku. You will work with experts in the field of x-ray crystallography, automation, cryogenics and software development at Rigaku as well some of the world’s leading scientists in the field. You will need excellent communication skills and a desire to travel. This position is located at our North American headquarters in The Woodlands, TX, just north of Houston. Rigaku offers a rewarding work environment and excellent benefits. Rigaku offers a full line of instrumentation and automation hardware and software products that cover the entire crystallographic pipeline from crystal growth through structure solution. Please send resumes by mail to Rigaku Americas, 9009 New Trails Dr., The Woodlands, TX 77381-5209, Attention: Human Resources, by fax to 281-364-3628 or email to [EMAIL PROTECTED] Please paste the resume in the body of the email. Email containing attachments will not be accepted. Rigaku Americas is an EEO/AA Employer M/F/D/V.
