[ccp4bb] Postdoctoral position in Cambridge UK

[Marko Hyvonen]

Dear colleagues,


We have an exciting postdoctoral opportunity in our group to work with Bicycle 
Therapeutics. Looking for motivated candidates with green protein fingers and 
experience in structural biology.

12 months post to start with. Details of the position and link to our 
application portal found at https://www.jobs.cam.ac.uk/job/44349/

Feel free to email me with questions.

Do spread the word! (also in Twitter/X and LinkedIn)🙏🏻

Best wishes, Marko

-
Marko Hyvönen
Professor of Protein Biochemistry
Department of Biochemistry
University of Cambridge
mh...@cam.ac.uk
https://hyvonen.bioc.cam.ac.uk
@HyvonenGroup





To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

[ccp4bb] Postdoctoral position in University of Cambridge

[Marko Hyvonen]

Dear Colleagues,

We have a 12 month postdoctoral position in an exciting collaborative project 
with Apollo Therapeutics to use fragment-based methods on an new target, to 
validate existing hits and to establish crystallographic system to support hit 
validation and optimisation. Should the project be successful, funding could be 
extended further.

More details and links to application portal from here: 
https://www.jobs.cam.ac.uk/job/39691/

Happy to answer informal queries.

Best wishes, Marko

-
Marko Hyvonen
Department of Biochemistry
University of Cambridge
mh...@cam.ac.uk<mailto:mh...@cam.ac.uk>
https://hyvonen.bioc.cam.ac.uk
@HyvonenGroup




To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

[ccp4bb] Postdoctoral position to characterise novel plastic degrading enzymes

[Marko Hyvonen]

Dear Colleagues.

Still six days to apply for an exciting opening in our group for a postdoc to 
work on characterisation of new plastic degrading enzymes ("plastizymes") in a 
BBSRC-funded sLoLa project lead by Florian Hollfelder and in collaboration with 
Christine Orengo (UCL) and Rob Finn (EMBL-EBI).

We are looking for someone with significant expertise on producing hard to make 
proteins and in characterisation of these biochemically, biophysically and 
structurally (X-ray crystallography, NMR, cryo-EM are all as options). Some 
exciting chemical biology thrown into the mix as well.

More details and application from University of Cambridge job portal: 
https://www.jobs.cam.ac.uk/job/38770/

Feel free to drop a line if you have specific questions.

best, Marko
-----
Marko Hyvonen
Department of Biochemistry
University of Cambridge
mh...@cam.ac.uk<mailto:mh...@cam.ac.uk>
https://hyvonen.bioc.cam.ac.uk
@HyvonenGroup




To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

[ccp4bb] Rockimager (spares) looking for a new home

[Marko Hyvonen]

Hello all,

We are in the process of replacing our Formulatrix Rockimager system and 
getting rid of the old one. Don't have the exact specs at hand, but it is >10 
years old system with 1000 plate capacity and with both visible and UV imaging. 
It is in full working order, but beyond support by Formulatrix.

Happy to give all or parts of it if someone is in need of spares, otherwise it 
will just be disposed off as scrap metal (super wasteful).  We can dismantle as 
needed,  but  you need to arrange for shipping.

I realise now that I have failed to summarise, as promised, the outcome of my 
earlier request for opinions on replacement crystal imaging systems . The 
response to that was minimal, apart from a few very informative private 
replies. The take-home message was that for automated imaging systems 
Formulatrix is the only supplier anybody recommends. So, little serious 
competition.

Best, Marko

-----
Marko Hyvonen
Department of Biochemistry
University of Cambridge
mh...@cam.ac.uk<mailto:mh...@cam.ac.uk>
https://hyvonen.bioc.cam.ac.uk
@HyvonenGroup




To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

[ccp4bb] Assistant/Associate professor position open at the Department of Biochemistry, Cambridge (UK, obvs)

[Marko Hyvonen]

Dear Colleagues,

Could I draw your attention to a just opened position for an 
assistant/associate professor at our Department at the University of Cambridge 
(https://www.bioc.cam.ac.uk/).
The research in our Department covera a wide range of topics in biochemistry 
and we are particularly strong in structural biology with great facilities for 
cryo-EM, crystallography, NMR and biophysics: 
https://facilities.bioc.cam.ac.uk/.

More details on how to apply here: https://www.jobs.cam.ac.uk/job/38144/

Best, Marko

-
Marko Hyvonen
Department of Biochemistry
University of Cambridge
mh...@cam.ac.uk<mailto:mh...@cam.ac.uk>
https://hyvonen.bioc.cam.ac.uk
@HyvonenGroup




To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

[Marko Hyvonen]

Hello Eike,

A couple of comments on the AKTA Pures (while I have not experience with the 
BioRad systems).

We have a couple of AKTA Pures and we (and another lab close to us) had a 
catastrophic failure of the 3-wavelength detector "block". Price tag for the 
replacement part (apparently a black box  with nothing accessible for repair - 
happy to hear if this was misinformation!) was over £12,000. Someone commented 
that they had even more eye-watering price tag for an upgrade to triple 
wavelength. We switched to a single wavelength detector at £5k and I will get 
these from now on only.

I really dislike the database system used in new Unicorn for storing data etc: 
another black box. I much prefer the way older Unicorns worked, but maybe that 
is just me being old and grumpy and stuck in my ways. That reminds also that we 
tend to source computers separately. Much better systems available for less 
money and with MUCH smaller footprint.

The price tags on Pures (like almost everything else with Cytiva) are in my 
opinion a reflection of the market dominance. I wish there was more competition 
(bring back Biocad!). Service contracts with Cytiva are also ridiculously 
expensive - we have, luckily,  a very knowledgeable company here that does all 
the servicing and maintenance, at less than half the price.

Having just complained, I do like AKTA Pures and there is much to like in them. 
Time will tell if these will be as robust as the original "grey" AKTA 
purifiers. Several of ours Purifiers still running perfectly after over 15 
years. As long as the detector works and flow is accurate, the proteins elute 
just the same from the columns. However, once you have even one of the shinier 
systems around, those old ones start collecting dust very soon.

Hth, Marko


Marko Hyvonen
Department of Biochemistry
University of Cambridge
https://hyvonen.bioc.cam.ac.uk
@HyvonenGroup
mh...@cam.ac.uk<mailto:mh...@cam.ac.uk>




From: CCP4 bulletin board  On Behalf Of Schulz, 
Eike-Christian
Sent: 16 September 2022 08:40
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Dear all,

I am considering to purchase a chromatography system for routine protein 
purification. The device is supposed to be used in a multi-user environment, 
hence ease of use, ease of training and ease of maintenance is important. I am 
rather looking for a robust system people like to use than a system that comes 
with many bells and whistles that no one dares to touch.


·   Is there any particularly _bad_ experience with either system?


·   Is there any specific advantage of one system over the other?


·   Does anyone have experience about (long-term) stability / performance 
of the systems?


·   Do you think the higher price-point of the Aekta systems is justified?


With best regards,

Eike






To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=05%7C01%7Cmh256%40universityofcambridgecloud.onmicrosoft.com%7C043db79219b645ebdbcc08da97b6ebaf%7C49a50445bdfa4b79ade3547b4f3986e9%7C0%7C0%7C637989109134174601%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=rNqSReQp58Lkvak0MK8Yl3xk2Dqemq3cEkrytBmj8SQ%3D&reserved=0>



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

[ccp4bb] HFSP funding for Ukrainian scientists

[Marko Hyvonen]

Dear colleagues,
please see message below from Prof Laura Itzhaki.

HFSP has a new initiative to support Ukrainian scientists via supplements to 
current HFSP project grant holders and Fellows. We have 18 months on our HFSP 
project grant and would be happy to consider Ukrainian PhD students or postdocs 
with a background in experimental or computational structural biology/protein 
biophysics. This is a collaborative project between my group and the groups of 
Ivet Bahar (USA), Reuven Gordon (Canada) and Shang-Hua Yang (Taiwan) focusing 
on understanding and engineering allostery in tandem-repeat proteins. Please 
email ls...@cam.ac.uk for more information.

Laura Itzhaki
Dept of Pharmacology
University of Cambridge



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

[ccp4bb] Postdoctoral position in collaboration with Bicycle Therapeutics

[Marko Hyvonen]

A postdoctoral position in our group at the Department of Biochemistry in 
Cambridge (UK) to use crystallography and biophysics to study the mechanism of 
inhibition by bicyclic peptide inhibitors developed at Bicycle Therapeutics.

Great opportunity to work at the academia/industry interface for a talented 
structural biologists.

Application details from University job portal along  with contact details for 
queries: https://jobs.cam.ac.uk/job/34566/

Best, Marko

Marko Hyvonen
Department of Biochemistry
University of Cambridge
https://hyvonen.bioc.cam.ac.uk
@HyvonenGroup
mh...@cam.ac.uk




To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

[ccp4bb] Advice on crystal imaging systems

[Marko Hyvonen]

Dear colleagues,

We are looking to replace our aging crystal imaging system (Formulatrix Rock 
Imager). Any advice on the latest offerings? Anyone using JANSi UVEXps and 
happy to share their experiences?

Feel free to email directly as well, I'll post an anonymised & moderated 
summary of the responses back to CCP4bb (w/o comments that are asked to be kept 
confidential).

Thanks in advance, Marko
-----
Marko Hyvonen
Department of Biochemistry
University of Cambridge
https://hyvonen.bioc.cam.ac.uk
@HyvonenGroup
mh...@cam.ac.uk




To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

[ccp4bb] Postdoctoral position in structure-guided drug discovery

[Marko Hyvonen]

A postdoctoral position is available for 24 months in our group at the 
Department of Biochemistry, University of Cambridge.

We are looking for someone with a strong background in structural biology and 
biophysics to support inhibitor development against a complex metabolic enzyme 
in collaboration with the groups of Morten Grotli and Jan Boren at the 
University of Gothenburg, funded by a Wellcome Trust Innovator award.

Candidates with experience in mechanistic studies of enzymes using variety of 
biochemical, biophysical and structural biology (X-ray, cryo-EM and/or NMR) 
methods are particularly encouraged to apply as are those with previous 
expertise in structure-guided drug discovery. Beyond inhibitor design work, a 
major aim  of the project is to elucidate in detail how the target gets 
inhibited allosterically.

Our Department is well equipped for all aspects of this work, with 
crystallisation, NMR, Biophysics and cryo-EM facilities within the Department.

Details of the position are found on University of Cambridge online application 
portal at https://www.jobs.cam.ac.uk/job/32674/

Informal queries to me are most welcome.

Best, Marko

Marko Hyvonen
Department of Biochemistry
University of Cambridge
https://hyvonen.bioc.cam.ac.uk
@HyvonenGroup
mh...@cam.ac.uk




To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

[ccp4bb] Postdoctoral position in FBDD in Cambridge UK

[Marko Hyvonen]

Dear colleagues,

We have an opening in our group at the Department of Biochemistry, University 
of Cambridge,  for a postdoctoral researcher for 12 months to work on an 
exciting drug discovery project with Alborada Drug Discovery Institute 
(https://cambridge-ddi.alzheimersresearchuk.org/) to support their inhibitor 
design process with fragment-based approaches and with structural analyses.

We'd hope to find someone with expertise in this area already, be it in 
crystallography (we hope to run a large fragment screen against the target), 
NMR (ligand-observe fragment screens), biophysics (ITC, SPR, ...)  and/or 
chemistry, but significant support around as well.  Plenty of work to be done 
to understand how the inhibitors work, so experience in mechanistic analysis of 
proteins would be very useful.

Details from University of Cambridge application portal: 
https://www.jobs.cam.ac.uk/job/31927/

Happy to receive informal queries by email as well.

Please do spread the word towards any potential candidates.

Best, Marko


Marko Hyvonen
Department of Biochemistry
University of Cambridge
https://hyvonen.bioc.cam.ac.uk
@HyvonenGroup
mh...@cam.ac.uk




To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

Re: [ccp4bb] External: Re: [ccp4bb] AlphaFold: more thinking and less pipetting (?)

[Marko Hyvonen]

  
  
Hi Ian, 
  
  The data on Alphafold2 target RMSDs seems to be correct, but that
  "resolution around 2.5Å", makes no sense, I agree  - had not
  noticed that before. I can see that this has been raised in the
  Twitter feed comments to his post too.   
  
  I was highlighting this more for the alternative viewpoint on the
  discussion and also on the interesting detail on the resources
  needed/available (assuming correct!).
  
  Marko

On 08/12/2020 14:02, Ian Tickle wrote:


  
  

  Hi Marko
  
  
  I hope he hasn't confused resolution with RMSD error:
  
  
  "Just
  keep in mind that (1) a lower RMSD represents a better
  predicted structure, and that (2) most experimental
  structures have a resolution around 2.5 Å. Taking this
  into consideration, about a third (36%) of Group 427’s
  submitted targets were predicted with a root-mean-square
  deviation (RMSD) under 2 Å, and 86% were under 5 Å, with a
  total mean of 3.8 Å."
  
  

  Cheers
  

  --
  Ian
  

  


  
  
  
On Tue, 8 Dec 2020 at 13:51,
  Marko Hyvonen <mh...@cam.ac.uk> wrote:


   Here is another take on this
  topic, by Carlos Quteiral (@c_outeiral), from a
  non-crystallographer's point of view, covering many of the
  points discussed in this
  thread  (incl. an example
  of the model guiding correction of the experimental
  structure).
  
  https://www.blopig.com/blog/2020/12/casp14-what-google-deepminds-alphafold-2-really-achieved-and-what-it-means-for-protein-folding-biology-and-bioinformatics/
  
  Marko

On 08/12/2020 13:25, Tristan Croll wrote:


  
This is a number that needs to be interpreted with some
care. 2 Å crystal structures in general achieve an RMSD
of 0.2 Å on the portion of the crystal that's resolved,
including loops that are often only in well-resolved
conformations due to physiologically-irrelevant crystal
packing interactions. The predicted models, on the other
hand, are in isolation. Once you get to the level
achieved by this last round of predictions, that starts
making fair comparison somewhat more difficult*. Two
obvious options that I see: (1) limit the comparison
only to the stable core of the protein (in which case
many of the predictions have RMSDs in the very low
fractions of an Angstrom), or (2) compare ensembles
derived from MD simulations starting from the
experimental and predicted structure, and see how well
they overlap.
  

  
  -- Tristan
  
  
  * There's one more thorny issue
when you get to this level: it becomes more and more
possible (even likely) that the prediction gets some
things right that are wrong in the experimental
structure. 
  
  From: CCP4 bulletin
  board 
  on behalf of Ian Tickle 
  Sent: 08 December 2020 13:04
  To: CCP4BB@JISCMAIL.AC.UK
  
  Subject: Re: [ccp4bb] External: Re: [ccp4bb]
  AlphaFold: more thinking and less pipetting (?)
 
  
  

  

  
There was a little bit of press-release
  hype: the release stated "a score of
around 90 GDT is informally considered to be
competitive with results obtained from
experimental methods" and "our
latest AlphaFold system achieves a median
score of 92.4 GDT overall across all
targets. This means that our predictions
have an average error (RMSD) of
approximately 1.6 Angstroms,".

  
Experimental
  methods achieve an average error of around
   

Re: [ccp4bb] External: Re: [ccp4bb] AlphaFold: more thinking and less pipetting (?)

[Marko Hyvonen]

ne could explain the difference (if any) between Go and
the protein folding problem perhaps using the NP type
categories.
>>  
>> Colin
>>  
>>  
>>  
>> From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On
Behalf Of Isabel Garcia-Saez
>> Sent: 03 December 2020 11:18
>> To: CCP4BB@JISCMAIL.AC.UK
>> Subject: [ccp4bb] AlphaFold: more thinking and less
pipetting (?)
>>  
>> Dear all,
>>  
>> Just commenting that after the stunning performance
of AlphaFold that uses AI from Google maybe some of us we
could dedicate ourselves to the noble art of gardening,
baking, doing Chinese Calligraphy, enjoying the clouds pass
or everything together (just in case I have already prepared
my subscription to Netflix).
>>  
>> 
  https://www.nature.com/articles/d41586-020-03348-4
>>  
>> Well, I suppose that we still have the structures
of complexes (at the moment). I am wondering how the labs
will have access to this technology in the future (would it
be for free coming from the company DeepMind - Google?). It
seems that they have already published some code. Well,
exciting times. 
>>  
>> Cheers,
>>  
>> Isabel
>>  
>>  
>> Isabel Garcia-Saez              PhD
>> Institut de Biologie Structurale
>> Viral Infection and Cancer Group (VIC)-Cell
Division Team
>> 71, Avenue des Martyrs
>> CS 10090
>> 38044 Grenoble Cedex 9
>> France
>> Tel.: 00 33 (0) 457 42 86 15
>> e-mail: isabel.gar...@ibs.fr
>> FAX: 00 33 (0) 476 50 18 90
>> http://www.ibs.fr/
>>  
>>  
>> To unsubscribe from the CCP4BB list, click the
following link:
>> 
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
>> 
>>  
>> 
>> -- 
>> 
>> This e-mail and any attachments may contain
confidential, copyright and or privileged material, and are
for the use of the intended addressee only. If you are not
the intended addressee or an authorised recipient of the
addressee please notify us of receipt by returning the
e-mail and do not use, copy, retain, distribute or disclose
the information in or attached to the e-mail.
>> Any opinions expressed within this e-mail are those
of the individual and not necessarily of Diamond Light
Source Ltd.

>> Diamond Light Source Ltd. cannot guarantee that
this e-mail or any attachments are free from viruses and we
cannot accept liability for any damage which you may sustain
as a result of software viruses which may be transmitted in
or with the message.
>> Diamond Light Source Limited (company no. 4375679).
Registered in England and Wales with its registered office
at Diamond House, Harwell Science and Innovation Campus,
Didcot, Oxfordshire, OX11 0DE, United Kingdom
>>  
>> 
>>  
>> To unsubscribe from the CCP4BB list, click the
following link:
>> 
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
>> 
>> 
>> To unsubscribe from the CCP4BB list, click the
following link:
>> 
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
>> 
> 
> 
> To unsubscribe from the CCP4BB list, click the
following link:
> 
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
> 



To unsubscribe from the CCP4BB list, click the following
link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of 
  www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by 
  www.jiscmail.ac.uk, terms & conditions are
a

[ccp4bb] Postdoctoral fellowship in structure-guided drug discovery

[Marko Hyvonen]

Postdoctoral fellowship available in a collaborative structure-guided drug 
discovery project between my group and groups of Sharon Rossiter and Stewart 
Kirton at University of Hertfordshire, funded by the Hertfordshire Science 
Partnership Therapy Accelerator scheme.


Successful candidate will be part of a multidisciplinary team exploring novel 
inhibitors of a cancer drug target  protein. The position offers access to the 
state of the art laboratory facilities at the University of Cambridge and the 
University of Hertfordshire, plus the opportunity to work at the dynamic 
industry/academia interface of the  Stevenage BioScience Catalyst campus 
(http://www.stevenagecatalyst.com/). The postdoctoral fellow will develop and 
optimise biophysical methods for high-throughput screening of inhibitors, 
measurement of inhibitor-protein interactions, crystallographic elucidation of 
ligand-protein complexes and fragment-based approaches to discovering novel 
inhibitor scaffolds as a potential therapy for pancreatic cancer.


Applicants should possess (or about to obtain) a doctorate in biochemistry, 
chemistry, structural biology or a closely related subject. Experience in 
molecular cloning, protein expression, purification and characterisation, 
biophysical analysis of protein-ligand interactions and protein crystallography 
are essential for this post, previous experience in structure-guided drug 
discovery a definite advantage.


More details of the positions and on how to apply can be found through the link 
below and on the website of University of Hertfordshire (https://www.jobs.herts.ac.uk).


Informal enquiries can  be sent to Dr Sharon Rossiter (s.rossi...@herts.ac.uk) 
or myself.


http://www.jobs.ac.uk/job/BGL627/postdoctoral-research-fellow-in-structure-guided-drug-discovery/

Marko

--

Marko Hyvonen
Department of Biochemistry, University of Cambridge
mh...@cam.ac.uk
+44 (0)1223 766 044
@HyvonenGroup
http://hyvonen.bioc.cam.ac.uk

[ccp4bb] Postdoctoral position in drug discovery

[Marko Hyvonen]

A postdoctoral position for a structural biologists in a CRUK-funded project in 
the group of Dr Walid Khaled at the Department of Pharmacology, University of 
Cambridge in a collaborative project with my lab and with Prof Laura Itzhaki.


Candidates are expected to have experience in studying protein-protein 
interactions and/or a strong interest in cancer biology. The candidates will 
need to have strong experience in the following techniques: biophysical assays 
(eg. SPR), proteomics and biochemical assays. Experience with protein 
crystallography or NMR will also be desirable.


Informal enquiries should be made to Dr. Walid Khaled (wt...@cam.ac.uk).

Application details are found at http://www.jobs.cam.ac.uk/job/16025/. Closing 
date for applications is 26th January 2018.


best, Marko


--

Marko Hyvonen
Department of Biochemistry, University of Cambridge
mh...@cam.ac.uk
+44 (0)1223 766 044
@HyvonenGroup
http://hyvonen.bioc.cam.ac.uk

[ccp4bb] PhD studentships in tackling M. abscessus infections in Cystic Fibrosis

[Marko Hyvonen]

PhD studentships in a collaborative project "Tackling Mycobacterium abscessus 
infection in Cystic Fibrosis"


http://www.jobs.cam.ac.uk/job/3954/

(see "Position 3" for the structural biology project)

The Departments of Biochemistry and Medicine, University of Cambridge, are 
pleased to offer 3 PhD studentships, funded by a Strategic Research Centre Award 
from The Cystic Fibrosis (CF) Trust UK, to start in October 2014 or January 
2015. The area of research will be the study of the rapid growing nontuberculous 
mycobacterium (NTM) Mycobacterium abscessus, which has emerged as a major threat 
to individuals with CF, focusing on understanding pathogenesis (through cell 
biology and population-based whole genome sequencing) and developing novel 
therapeutics (through Fragment-Based Drug Development).


We invite applications from highly motivated, enthusiastic individuals for these 
studentships leading to PhDs in the University of Cambridge. We anticipate that 
the students will work closely together and have the opportunity to develop a 
broad range of expertise across the projects.


Position 1
Supervised by Dr Andres Floto, Cambridge Institute for Medical Research/ 
Department of Medicine, and Professor Julian Parkhill, Wellcome Trust Sanger 
Institute.


The student will use cell and molecular biology techniques to understand how 
macrophages interact with M. abscessus, define novel genetic determinants 
(informed through whole genome sequencing) regulating host restriction and 
mycobacterial virulence and identify novel therapeutics to stimulate autophagic 
killing.


Position 2
Supervised by Professor Julian Parkhill, Wellcome Trust Sanger Institute, and Dr 
Andres Floto, Cambridge Institute for Medical Research/ Department of Medicine


The student will use large-scale population-level genomics on local and global 
M. abscessus populations to identify emerging and transmitting clones, and 
predict determinants of virulence and transmissibility that can be tested in the 
in vitro and in vivo models. Building on our recent identification of 
patient-to-patient transmission and the emergence of dominant clones, the 
student will use phylogenetic analyses to investigate the adaption of M. 
abscessus to survival in the CF lung, and to transmission between hosts.


Position 3
Supervised by Professor Sir Tom Blundell and Dr Marko Hyvonen, Department of 
Biochemistry


The student will use fragment-based drug discovery (FBDD) approach involving (i) 
expression and characterisation of protein targets, (ii) use of a cascade of 
biophysical techniques, followed by protein X-ray crystallography to define the 
3D structures of the complexes, and (iii) knowledge of fragment binding to 
target proteins to select commercially available compounds that would likely 
give rise to higher affinities (SAR-by-catalogue). Once a sufficiently potent 
molecule has been developed, it efficacy will be tested in cellular assays.


Queries should be made to Dr Andres Floto (ar...@cam.ac.uk) (Position 1), Prof. 
Julian Parkhill (parkh...@sanger.ac.uk) (Position 2), Prof. Sir Tom Blundell 
(tl...@cam.ac.uk) and Dr Marko Hyvonen (mh...@cam.ac.uk) (Position 3).


Candidates will be expected to have a strong CV having completed, or about to 
complete, a degree relevant to cell biology, microbiology, bioinformatics or 
structural biology but we shall consider all suitably qualified applicants.


The funding for UK/EU nationals covers stipend, University Composition fees and 
College fees for 3 years, with the potential for an extension to a fourth year. 
Overseas applicants are eligible to apply, but would be required to self-fund 
the cost difference of University Composition fees for overseas students 
compared with UK/EU students (approximately £15,700 per year for 3 years).


Applications should be sent as a single pdf document and should include a 
curriculum vitae and contact details (including email addresses) of two academic 
referees, together with a single page outline of why the applicant wishes to 
apply for , this studentship programme. Applications should be sent by email to 
cf-...@bioc.cam.ac.uk, with a subject line indicating which position the 
application is for.


Application deadline: 28th June 2014. Short-listed applicants will be invited to 
interview.


PhD expected start date: 01 October 2014 or January 2015


 --

 Marko Hyvonen
 Department of Biochemistry, University of Cambridge
 mh...@cam.ac.uk
 http://www-cryst.bioc.cam.ac.uk/groups/hyvonen
 tel:+44-(0)1223-766 044

[ccp4bb] Sequence for pBS24Ub

[Marko Hyvonen]

Apologies for a marginally out of topic request, but would anyone have the 
complete DNA sequence for pBS24Ub vector? Any format will do, and even a close 
derivative of it would be useful. Published in Sabin et al, Nature 
Biotechnology 7, 705 - 709 (1989) 
http://www.nature.com/nbt/journal/v7/n7/abs/nbt0789-705.html)


thanks, Marko

 _

 Marko Hyvonen
 Department of Biochemistry, University of Cambridge
 ma...@cryst.bioc.cam.ac.uk
 http://www-cryst.bioc.cam.ac.uk/groups/hyvonen

[ccp4bb] Post-doctoral fellowship in fragment-based drug discovery

[Marko Hyvonen]

Dear colleagues,

below is an advert for an postdoc position in my group.

best, Marko

Applications are invited from post-doctoral scientists with expertise in protein 
crystallography to join the group of Dr Marko Hyvonen 
(http://www-cryst.bioc.cam.ac.uk/groups/hyvonen/) at the Department of 
Biochemistry as part of a multidisciplinary drug discovery programme between the 
Departments of Chemistry (Prof Chris Abell and Dr David Spring) and Biochemistry 
(Dr Marko Hyvonen and Prof Tom Blundell) and the MRC Cancer Unit (Prof Ashok 
Venkitaraman and Dr Grahame McKenzie) at the University of Cambridge.


Candidates must have experience in all aspects from protein crystallography from 
protein expression and purification through crystallisation and structure 
determination. Previous experience in high-throughput crystallography, in 
scripting and automation of crystallographic work-flow is highly desirable and 
background in drug discovery would also be advantageous.


Candidates must have a PhD in structural biology and relevant post-doctoral 
experience is desirable, as is a track record of achievement exemplified by 
academic publications and/or industrial experience. Applicants will be expected 
to work closely and interactively with colleagues in different disciplines, to 
have good communications skills and the ability to work under pressure to meet 
deadlines.


Informal enquiries can be sent to Dr Marko Hyvonen (mh...@cam.ac.uk).

Fixed-term: The funds for this post are available for 15 months in the first 
instance.


More details of the application process can be found at
http://www.jobs.cam.ac.uk/job/2689/

 _

 Marko Hyvonen
 Department of Biochemistry, University of Cambridge
 ma...@cryst.bioc.cam.ac.uk
 http://www-cryst.bioc.cam.ac.uk/groups/hyvonen
 tel:+44-(0)1223-766 044
 mobile: +44-(0)7796-174 877
 fax:+44-(0)1223-766 002
 --

Re: [ccp4bb] Rec- variant of E coli B

[Marko Hyvonen]

Hi Mark,

BLR(DE3) should be what you are after.

http://www.emdmillipore.com/life-science-research/blrde3-competent-cells/EMD_BIO-69053/p_yYub.s1Ol18AAAEjORl9.zLX

hth, Marko

On Wed, 27 Mar 2013, Mark J van Raaij wrote:


Dear All,
we were wondering if knows of an Escherichia coli B strain that is Rec 
deficient (Rec-).
We want to compare certain properties of E coli B and K12, and for the 
experiment it would be best to use a Rec- variant.
We have TOP10, which is a K12 derivative that is Rec-, but derivatives of B 
like BL21 are Rec+.
Have a happy Easter,
Mark

Mark J van Raaij
Lab 20B
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://www.cnb.csic.es/~mjvanraaij




 _

 Marko Hyvonen
 Department of Biochemistry, University of Cambridge
 ma...@cryst.bioc.cam.ac.uk
 http://www-cryst.bioc.cam.ac.uk/groups/hyvonen
 tel:+44-(0)1223-766 044
 mobile: +44-(0)7796-174 877
 fax:+44-(0)1223-766 002
 --

Re: [ccp4bb] sequence format conversion

[Marko Hyvonen]

Surely a sequence analysis tools are the easiest way to do it.

I'd recommend EMBOSS (open source and runs nicely on most platforms - the 
"ccp4" of sequence analysis for me at least) 
http://emboss.sourceforge.net/


Seqret (SEQuence RETurn) program:

seqret -out test.seq -osformat gcg test.fasta

Marko

PS. fasta format needs ">" as a first line with (optional) description in 
the input file. And not sure what amino acids "b" and "j" would get 
converted to :-)


On Tue, 8 May 2012, Francois Berenger wrote:


More seriously, there is the babel command from Open Babel
in case the second format you show has a known name.

On 05/08/2012 04:46 PM, Francois Berenger wrote:

Hello,

The tool is called awk.
There is also another tool called Perl, but I won't recommend it.

Regards,
F.

On 05/08/2012 04:02 PM, K Singh wrote:

Dear All
I was looking for a script or an informatics tool enabling me to
change the sequence from FASTA format to something like following:


FASTA FORMAT

abcdefghijklmnopqrstuvwxyz

to

1 abcde fghij
11 klmno pqrst
21 uvwxy z


Many thanks in advance

Regards
Kris





 _

 Marko Hyvonen
 Department of Biochemistry, University of Cambridge
 ma...@cryst.bioc.cam.ac.uk
 http://www-cryst.bioc.cam.ac.uk/groups/hyvonen
 tel:+44-(0)1223-766 044
 mobile: +44-(0)7796-174 877
 fax:+44-(0)1223-766 002
 --

[ccp4bb] Postdoctoral research associate in fragment-based inhibitor design

[Marko Hyvonen]

We are seeking to appoint a Research Associate in the group of Dr Marko 
Hyvonen to develop chemical tools against signalling proteins using 
fragment-based drug discovery methods. The post is part of a Wellcome 
Trust funded, multi-disciplinary programme between Departments of 
Biochemistry (Prof Tom Blundell and Dr Marko Hyvonen), Chemistry (Prof 
Chris Abell and Dr David Spring) and the Hutchison/MRC Research Centre 
(Prof Ashok Venkitaraman and Dr Grahame McKenzie).


Experience in all aspects of protein crystallography from construct design 
to structure refinement is essential. Experience with high-throughput 
crystallography, scripting and automation of crystallographic work-flow is 
highly desirable, experience with biophysical techniques such as ITC and 
Biacore an additional advantage.


Informal enquiries about the post can be sent to Dr Marko Hyvonen at
ma...@cryst.bioc.cam.ac.uk.

Closing Date: 11 April 2012

Information on how to apply: http://www.jobs.cam.ac.uk/job/-14591/

best, Marko

 _

 Marko Hyvonen
 Department of Biochemistry, University of Cambridge
 ma...@cryst.bioc.cam.ac.uk
 http://www-cryst.bioc.cam.ac.uk/groups/hyvonen
 tel:+44-(0)1223-766 044
 mobile: +44-(0)7796-174 877
 fax:+44-(0)1223-766 002
 --

[ccp4bb] Postdoctoral position in fragment-based inhibitor design

[Marko Hyvonen]

Postdoctoral position in protein crystallography in the group of Dr Marko 
Hyvonen, at the Department of Biochemistry, University of Cambridge, to 
develop chemical tools against signalling proteins using fragment-based 
drug discovery methods. The post is part of a Wellcome Trust funded, 
multi-disciplinary programme between Departments of Biochemistry (Dr Marko 
Hyvonen Prof Tom Blundell) Chemistry (Prof Chris Abell and Dr David 
Spring) and the Hutchison/MRC Research Centre (Prof Ashok Venkitaraman and 
Dr Grahame McKenzie).


Experience in all aspects of protein crystallography from construct design 
to structure refinement is essential. Experience with high-throughput 
crystallography, scripting and automation of crystallographic work-flow is 
highly desirable, experience with biophysical techniques such as ITC and 
Biacore advantage.


Further details at
http://www.admin.cam.ac.uk/offices/hr/jobs/vacancies.cgi?job=8752

Informal enquires to Marko Hyvonen by email: ma...@cryst.bioc.cam.ac.uk

 _

 Marko Hyvonen
 Department of Biochemistry, University of Cambridge
 ma...@cryst.bioc.cam.ac.uk
 http://www-cryst.bioc.cam.ac.uk/groups/hyvonen
 tel:+44-(0)1223-766 044
 mobile: +44-(0)7796-174 877
 fax:+44-(0)1223-766 002
 --

[ccp4bb] Postdoctoral positions in fragment-based drug discovery projects

[Marko Hyvonen]

A number of post-doctoral positions are available in the group of Marko 
Hyvonen, at the Department of Biochemistry, University of Cambridge.


These positions are in multidisciplinary projects aimed at developing 
novel therapeutic agents and chemical tools against protein-protein 
interactions in clinically and scientifically relevant targets using 
fragment-based methods.


We are inviting applications from motivated scientists in the following 
areas:


* Protein crystallographers with expertise in high-throughput 
crystallography, data collection, structure refinement. Experience in 
scripting and automation of crystallographic work-flow is desirable.


* Biochemists /Molecular biologists with expertise in protein expression, 
engineering and purification, and in protein crystallisation.


* Biophysicists to apply and develop ITC, SPR and/or NMR methods for 
fragment screening and small molecule validation against selected 
targets.


Further details and information on how to apply can be found at
http://www.admin.cam.ac.uk/offices/hr/jobs/vacancies.cgi?job=6331

Informal queries are welcome, but applications and CVs  must be sent 
through the route described in the link above.


best wishes, Marko
 _

 Marko Hyvonen
 Department of Biochemistry, University of Cambridge
 ma...@cryst.bioc.cam.ac.uk
 http://www-cryst.bioc.cam.ac.uk/groups/hyvonen
 tel:+44-(0)1223-766 044/760 468
 mobile: +44-(0)7796-174 877
 fax:+44-(0)1223-766 002
 --

Re: [ccp4bb] Meaning of sigma level of electron density map?

[Marko Hyvonen]

 
> See: that is an email address I understand .. sort of ... Is that
> especially big or very small (in rms/sigma units)?

Good question. The calculation of the sigma level is local in this case, 
and not uniform across the whole map of the world. In Britain, size 44 
corresponds to 9.5 sigma units, where as in the US it would be ca. 10 
sigmas. Can be confusing, but the rumour has it that the Japanese use 
absolute values for this, which in this case is just under 29cm. Much more 
sensible, I think.

Marko


> ***
> * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com
> *
> *  Global Phasing Ltd.
> *  Sheraton House, Castle Park 
> *  Cambridge CB3 0AX, UK
> *--
> * BUSTER Development Group  (http://www.globalphasing.com)
> ***
> 


 _____

 Marko Hyvonen
 Department of Biochemistry
 University of Cambridge
 http://www-cryst.bioc.cam.ac.uk/~marko
 tel:  +44-(0)1223-766 044 / 760 468, fax: 766 002
 ___

Re: [ccp4bb] Meaning of sigma level of electron density map?

[Marko Hyvonen]

> Wouldn't it be good manner to show your name on posts to the ccp4bb?
> There is no need for shoe size or bank account, but otherwise this
> turns into a big anonymous mailing list (and this is not quite fair to
> all the helpful people making their name visible - at the danger of
> geting quoted 5 years later or some future employer or funding agency
> checking on their past record).

I fully agree. 

Marko


Marko Hyvonen
University of Cambridge
[EMAIL PROTECTED]

Re: [ccp4bb] MSE

[Marko Hyvonen]

> 
> % egrep '^CRYST|^SCALE|^ATOM|^HETATM.{11}(MSE|PTR|SEP|TPO)' your.pdb >
> standard_residues_only.pdb

I think the original request was to extract particular chains, so 
extending the above further to select (say) chain A

egrep '^CRYST|^SCALE|^ATOM.{17}A |^HETATM.{11}(MSE|PTR|SEP|TPO) A' your.pdb > 
standard_residues_in_chainA.pdb 

Marko

> -- Ian
> 
> > -Original Message-
> > From: [EMAIL PROTECTED] 
> > [mailto:[EMAIL PROTECTED] On Behalf Of Phil Evans
> > Sent: 30 August 2007 15:45
> > To: CCP4BB@JISCMAIL.AC.UK
> > Subject: MSE
> > 
> > 
> > As an aside, does anyone understand why MSE is not an amino-acid?
> > Phil
> > 
> > 
> > On 30 Aug 2007, at 15:31, Clemens Vonrhein wrote:
> > 
> > > If your PDB file conforms to standard
> > >
> > >   http://www.wwpdb.org/documentation/format23/sect9.html#ATOM
> > >
> > > you could do
> > >
> > >   % egrep "^CRYST|^SCALE|^ATOM" your.pdb > 
> > standard_residues_only.pdb
> > >
> > > You'll miss the 'non-standard' Se-MET residue 'MSE' ;-)
> > 
> > 
> 
> 
> Disclaimer
> This communication is confidential and may contain privileged information 
> intended solely for the named addressee(s). It may not be used or disclosed 
> except for the purpose for which it has been sent. If you are not the 
> intended recipient you must not review, use, disclose, copy, distribute or 
> take any action in reliance upon it. If you have received this communication 
> in error, please notify Astex Therapeutics Ltd by emailing [EMAIL PROTECTED] 
> and destroy all copies of the message and any attached documents. 
> Astex Therapeutics Ltd monitors, controls and protects all its messaging 
> traffic in compliance with its corporate email policy. The Company accepts no 
> liability or responsibility for any onward transmission or use of emails and 
> attachments having left the Astex Therapeutics domain.  Unless expressly 
> stated, opinions in this message are those of the individual sender and not 
> of Astex Therapeutics Ltd. The recipient should check this email and any 
> attachments for the presence of computer viruses. Astex Therapeutics Ltd 
> accepts no liability for damage caused by any virus transmitted by this 
> email. E-mail is susceptible to data corruption, interception, unauthorized 
> amendment, and tampering, Astex Therapeutics Ltd only send and receive 
> e-mails on the basis that the Company is not liable for any such alteration 
> or any consequences thereof.
> Astex Therapeutics Ltd., Registered in England at 436 Cambridge Science Park, 
> Cambridge CB4 0QA under number 3751674
> 

 
 _
 
 Marko Hyvönen
 Department of Biochemistry
 University of Cambridge
 http://www-cryst.bioc.cam.ac.uk/~marko
 tel:  +44-(0)1223-766 044 / 760 468, fax: 766 002
 ___

Re: [ccp4bb] Water is about 60% everywhere

[Marko Hyvonen]

> 
> Are there any methods that allow one to input such ions as possible
> candidates?  What would be ideal is to have a program that says something
> like "The density peak at x,y,z, has a .0973 chance of being real.  It is
> only 2.2 Å from N7 of G12, precluding water.  Based on the mother liquor
> ingredient list you provided, there is a 0.75 chance that this is Mg++,
> 0.25 chance it is Na+ (or whatever), based on a
> crystallographically-derived library of 40,000 structural variants of the
> ribosome.

Not quite what you ask for, but WASP does something along those lines. 

From the output of the program: 

#
#
 
WASP  ---  WAter Screening Program   
 
Version 1.0   1995   
 
 Copyright (c)  Murad Nayal & Enrico Di Cera  1995   
 
 
  This program will take a Protein Data Bank structural file with solvent
  electron density peaks designated as water oxygens. Based on simple
  structural criteria it will pick out the peaks that could be metal ions
  (Na+, Li+, Mg+2, Ca+2). The chosen peaks are subsequently written to 4 
  pdb-formatted files one for each ion. The temperature factor field is  
  replaced by the calculated ion-specific valence of that solvent peak.  
 
  Reference: Nayal, M., & Di Cera, E. (1996) J. Mol. Biol. 256: 228-234  
 
#
#

See http://biochem.wustl.edu/~enrico/


Marko


> 
> Bill
> 

 
 _
 
 Marko Hyvönen
 Department of Biochemistry
 University of Cambridge
 http://www-cryst.bioc.cam.ac.uk/~marko
 tel:  +44-(0)1223-766 044 / 760 468, fax: 766 002
 ___

Re: [ccp4bb] Movie for Powerpoint in windows

[Marko Hyvonen]

Hi Ibrahim, 

I have used pretty much the same options for mencoder as you.

mencoder "mf://myfile*.*[0-9].png" -mf fps=18 -o mymovie.mpg -ovc lavc 
-lavcopts vcodec=msmpeg4v2:vbitrate=9000

(and yes, 18fps is on the slow side, but in my opinion sufficient)

Several people have played these movies in their powerpoints successfully,
but I did have some trouble in the beginning changing between WinXP to
win2000, but I recall using version 2 of msmpeg sorted that out. 

But perhaps more crucially I also had problems with the same movie file
depending on the extension it had (I think I had .avi intially and that
failed) - this might have to do with default application for that file
type. I am guessing you should have windows mediaplayer as default for
these movie to work well in powerpoint - that's what I have and have not
dared to fiddle with it now that everything works Ok.

cheers, Marko

On Fri, 25 May 2007, Ibrahim M. Moustafa wrote:

> Hi all,
> 
>I'm trying to make a movie for a powerpoint presentation but going through
> some problems.
> 
>   I can make the frames in Pymol, so I have the series of .png files; no
> problem in that. The problem is to get a movie (.avi) so it can be inserted
> into the powerpoint.
> 
>   Googling showed a nice thread in ccp4bb and it is really helpful; but still
> can't make what I want exactly.
> 
>I can make a movie.mov which can be played by Quicktime or realplayer;
> however, I don't like the way of playing the movie using other program during
> the presentation! I want the movie to play in the same slide, the case when
> you insert a movie, which can be played by the windows media player, into the
> powerpoint.
> 
> Making the movie using the windows movie maker is not nice (slow,
> sluggish!may be there are ways to make it nicer! which I dunno!).
> 
>  Then I installed Mencoder and issued the command:
> 
>mencoder mf://*.png type=png -o output.avi -ovc lavc -lavcopts
> vcodec=msmpeg4v2:vbitrate=9000
> 
>  I tried various vcodec= values. I still unable to insert the .avi into the
> powerpoint. Windows media player cannot open the file either!
> 
>   I think there is something missing here!! I'd be appreciated if anyone can
> share his/her experience in making a movie that will play as part of the slide
> in the powerpoint on a PC laptop.
> 
>   P.S. I know that there might be other ways and a lot of options like
> CCP4mg..etc. But I don't want to spend time making the figures again. I
> already have the figures done in Pymol.
> 
>   thanks for your help in advance.
> 
>   thanks,
>  Ibrhaim
> 
> 
> 
> 
> --
> Ibrahim M. Moustafa, Ph.D.
> Biochemistry and Molecular Biology Dept.
> 201 Althouse Lab., Uinversity Park
> Pennsylvania State University, PA16802
> 
> Tel.  (814)863-8703
> Fax. (814)865-7927
> 
> --  

 
 _
 
 Marko Hyvönen
 Department of Biochemistry
 University of Cambridge
 http://www-cryst.bioc.cam.ac.uk/~marko
 tel:  +44-(0)1223-766 044 / 760 468, fax: 766 002
 ___