Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-19 Thread Jeff Christensen 
We've been using the Mitegen MicroRT product for a while and it works well,
but I'm anxious to try Filip Van Petegem's paratone oil method.  I've used
paratone as a cryo before, and spent a lot of time trying to wick away the
liquid around the crystal - in the case of room-temp mounting the
crystallant is desireable!  

Thanks for the tip,

Jeff

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-19 Thread Juan Sanchez Weatherby 

Dear Cedric,

Another possible method for RT data collection is the new humidifier 
device HC1b developed at the EMBL that is now available for use at the 
ESRF (see links below). It works in a very similar way to the FMS but a 
little easier to use and can be used at the ESRF with very little 
hassle. For availability please contact the ESRF and for technical 
information please let me know. My suggestion though for crystals that 
move while collecting is to use micromesh loops (either MiTeGen or 
Molecular Dimensions) instead of using standard loops. If you wick the 
mother liquor out once you've mounted them you get lots of contact 
points with the crystal and thus less slipping. If you are going to 
collect at a synchrotron source you may also want to reduce your flux 
(attenuating) and only use one pass so that your exposure time will be 
increased and thus your angular speed when collecting will be slower 
avoiding movement.


But no matter what you do you may still get some slipping, you simply 
cannot fully avoid it totally when working at RT. So you may have to 
take a wedge of good frames and scale separately from other wedges and 
then merge everything together. You should also tell your processing 
software to correct for slippage to get better scaling. Furthermore, you 
may just have to collect different crystals take the best bits and merge 
it all together; this is something that you may have to do anyway 
considering radiation damage at this temperatures.



Linkhttp://www.esrf.fr/UsersAndScience/Experiments/MX/special-setup

I hope this helps,

Regards,

Juan


cedric bauvois wrote:

Dear CCP4ers,

in their paper entitled " Using cryoloops for X-ray data collection 
from protein crystals at room temperature: A simple applicable method" 
( *Journal of Crystal Growth* 

Volume 281, Issues 2-4 
, 
1 August 2005, Pages 592-595.), the authors present a way to mount 
crystals using "a cryoloop accompanied by a glass capillary cap" (see 
abstract below).

Do you know if any commercial version of such system are now available ?

Abstract: Although cryoloops are now routinely used for X-ray data 
collection from protein crystals in cryocooling condition, it is still 
necessary to collect X-ray diffraction data from protein crystals at 
room temperature under such circumstances as to find resolution limit 
and/or to avoid damage of protein crystals at cryogenic temperature 
(e.g. 100 K). Here, we show that a cryoloop, which is accompanied by a 
glass capillary cap to maintain humid environment of crystal in the 
cryoloop, can be used not only to examine protein or non-protein 
crystals but also to collect X-ray diffraction data for structural 
analysis from protein crystals at room temperature. The size of 
cryoloop should be carefully chosen so that the crystal does not move 
in the cryoloop. This crystal mounting method can be time-saving 
compared to the traditional method to mount a crystal in a glass 
capillary tube.



Many thanks

--
Dr. Cedric Bauvois
Cristallographie des protéines
Institut de Recherches Microbiologiques JM Wiame -IRMW
Av E. Gryzon 1, 1070 Brussels (Belgium)  
tél: +32 (0)2 5273634

fax: +32 (0)2 5267273



--
Juan Sanchez-WEATHERBY

Tel:33 (0) 47620 7266

Website: http://www.embl.fr


***
Postal address:

EMBL Grenoble
6 rue Jules Horowitz, BP 181
38042 Grenoble Cedex 9
FRANCE
***
Delivery address: 


EMBL c/o ILL, Polygone Scientifique
6 Rue Jules Horowitz
38042 Grenoble  Cedex 9
France 
***

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-16 Thread Filip Van Petegem 
Dear Cedric,

We use a much easier test for mounting crystals at room temperature:
just coat the crystal with paratone oil and mount your crystal in a standard
cryoloop.  The oil will slow down evaporation enough - no special tools
required.  You don't even need to remove all the liquid as you would do for
flash-cooling the crystal in paratone.   And a major advantage: you can use
the same crystal to collect under cryo-conditions and directly compare the
impact of cooling the crystal...

Cheers

Filip Van Petegem


-- 
Filip Van Petegem, PhD
Assistant Professor
The University of British Columbia
Dept. of Biochemistry and Molecular Biology
2350 Health Sciences Mall - Rm 2.356
Vancouver, V6T 1Z3

phone: +1 604 827 4267
email: filip.vanpete...@gmail.com
http://crg.ubc.ca/VanPetegem/


On Fri, Jan 16, 2009 at 6:27 AM, cedric bauvois  wrote:

> Dear CCP4ers,
>
> in their paper entitled " Using cryoloops for X-ray data collection from
> protein crystals at room temperature: A simple applicable method" ( *Journal
> of Crystal Growth* 
> Volume 281, Issues 
> 2-4,
> 1 August 2005, Pages 592-595.), the authors present a way to mount crystals
> using "a cryoloop accompanied by a glass capillary cap" (see abstract
> below).
> Do you know if any commercial version of such system are now available ?
>
> Abstract: Although cryoloops are now routinely used for X-ray data
> collection from protein crystals in cryocooling condition, it is still
> necessary to collect X-ray diffraction data from protein crystals at room
> temperature under such circumstances as to find resolution limit and/or to
> avoid damage of protein crystals at cryogenic temperature (e.g. 100 K).
> Here, we show that a cryoloop, which is accompanied by a glass capillary cap
> to maintain humid environment of crystal in the cryoloop, can be used not
> only to examine protein or non-protein crystals but also to collect X-ray
> diffraction data for structural analysis from protein crystals at room
> temperature. The size of cryoloop should be carefully chosen so that the
> crystal does not move in the cryoloop. This crystal mounting method can be
> time-saving compared to the traditional method to mount a crystal in a glass
> capillary tube.
>
> Many thanks
>
> --
> Dr. Cedric Bauvois
> Cristallographie des protéines
> Institut de Recherches Microbiologiques JM Wiame -IRMW
> Av E. Gryzon 1, 1070 Brussels (Belgium)
> tél: +32 (0)2 5273634
> fax: +32 (0)2 5267273
>

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-16 Thread Kris Tesh 
The Free Mounting System (FMS) from Proteros Biostructures can also be a 
tool for this.  As long as it does not have highly volatile components in 
the crystalization conditions, room temperature testing is not too 
difficult.  There are other suppliers of humidity control devices which 
are available with lesser accuracy, but may still be useful...if you can 
get a little time on one.

Kris
-
Kris F. Tesh, Ph D
Director, Macromolecular Products
Rigaku Americas Corporation
9009 New Trails Drive
The Woodlands, TX  77381  USA
001 281 362 2300 x 144 



From:
Nobuhisa Watanabe 
To:
CCP4BB@JISCMAIL.AC.UK
Date:
01/16/2009 09:55 AM
Subject:
Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at 
room temperature



If you just want to check crystals at room temperature, we have
developed a "Direct Ex Plate" after Acta Cryst. D58(10), 1527-1530
(2002).

Please check,
http://www.labo.co.jp/contents/direct_ex.html
(Sorry for Japanese)

Nobuhisa Watanabe
===
Synchrotron Radiation Research Center
Department of Biotechnology and Biomaterial Chemistry,
Graduate School of Engineering
Nagoya University
Furo-cho Chikusa-ku, Nagoya 4648603 Japan
Email: nobuh...@nagoya-u.jp
Fax: +81-52-789-5286

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-16 Thread Nobuhisa Watanabe 
If you just want to check crystals at room temperature, we have
developed a "Direct Ex Plate" after Acta Cryst. D58(10), 1527-1530
(2002).

Please check,
http://www.labo.co.jp/contents/direct_ex.html
(Sorry for Japanese)

Nobuhisa Watanabe
===
Synchrotron Radiation Research Center
Department of Biotechnology and Biomaterial Chemistry,
Graduate School of Engineering
Nagoya University
Furo-cho Chikusa-ku, Nagoya 4648603 Japan
Email: nobuh...@nagoya-u.jp
Fax: +81-52-789-5286

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-16 Thread Van Den Berg, Bert 
Hi Cedric,
 
I haven't read this paper, but there's already a system available for roomtemp 
data collection that works quite well. Check out 
http://www.mitegen.com/products/micrort/micrort.shtml
Instead of a capillary they use thin polyester tubing that you slide over 
(special) bases so that everything is reasonably airtight. It apparently has 
also less background scatter compared to capillaries.
Compared to the old ways of cutting capillaries etc this works like a breeze.
 
Cheers, Bert (not affiliated to mitegen in any way, except as a customer...).
 
Bert van den Berg
University of Massachusetts Medical School
Program in Molecular Medicine
Biotech II, 373 Plantation Street, Suite 115
Worcester MA 01605
Phone: 508 856 1201 (office); 508 856 1211 (lab)
e-mail: bert.vandenb...@umassmed.edu
http://www.umassmed.edu/pmm/faculty/vandenberg.cfm



From: CCP4 bulletin board on behalf of cedric bauvois
Sent: Fri 1/16/2009 9:27 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cryoloops for X-ray data collection from protein crystals at 
room temperature


Dear CCP4ers,

in their paper entitled " Using cryoloops for X-ray data collection from 
protein crystals at room temperature: A simple applicable method" ( Journal of 
Crystal Growth <http://www.sciencedirect.com/science/journal/00220248> 
Volume 281, Issues 2-4 
<http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%235302%232005%23997189997%23601824%23FLA%23&_cdi=5302&_pubType=J&view=c&_auth=y&_acct=C26678&_version=1&_urlVersion=0&_userid=532047&md5=9a4e7b2fc158c6d2396925c79d995e3d>
 , 1 August 2005, Pages 592-595.), the authors present a way to mount crystals 
using "a cryoloop accompanied by a glass capillary cap" (see abstract below).
Do you know if any commercial version of such system are now available ?


Abstract: Although cryoloops are now routinely used for X-ray data collection 
from protein crystals in cryocooling condition, it is still necessary to 
collect X-ray diffraction data from protein crystals at room temperature under 
such circumstances as to find resolution limit and/or to avoid damage of 
protein crystals at cryogenic temperature (e.g. 100 K). Here, we show that a 
cryoloop, which is accompanied by a glass capillary cap to maintain humid 
environment of crystal in the cryoloop, can be used not only to examine protein 
or non-protein crystals but also to collect X-ray diffraction data for 
structural analysis from protein crystals at room temperature. The size of 
cryoloop should be carefully chosen so that the crystal does not move in the 
cryoloop. This crystal mounting method can be time-saving compared to the 
traditional method to mount a crystal in a glass capillary tube.


Many thanks 


-- 
Dr. Cedric Bauvois
Cristallographie des protéines
Institut de Recherches Microbiologiques JM Wiame -IRMW
Av E. Gryzon 1, 1070 Brussels (Belgium)  
tél: +32 (0)2 5273634
fax: +32 (0)2 5267273

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-16 Thread Edward A. Berry 

cedric bauvois wrote:

Dear CCP4ers,

in their paper entitled " Using cryoloops for X-ray data collection from 
protein crystals at room temperature: A simple applicable method" ( 
*Journal of Crystal Growth* 

Volume 281, Issues 2-4 
, 
1 August 2005, Pages 592-595.), the authors present a way to mount 
crystals using "a cryoloop accompanied by a glass capillary cap" (see 
abstract below).

Do you know if any commercial version of such system are now available ?



Sounds like the Mi-te-gen MicroRT method - only that uses polyester capiliary
which scatters xrays less than the glass:
http://www.mitegen.com/products/micrort/micrort.shtml


Abstract: Although cryoloops are now routinely used for X-ray data 
collection from protein crystals in cryocooling condition, it is still 
necessary to collect X-ray diffraction data from protein crystals at 
room temperature under such circumstances as to find resolution limit 
and/or to avoid damage of protein crystals at cryogenic temperature 
(e.g. 100 K). Here, we show that a cryoloop, which is accompanied by a 
glass capillary cap to maintain humid environment of crystal in the 
cryoloop, can be used not only to examine protein or non-protein 
crystals but also to collect X-ray diffraction data for structural 
analysis from protein crystals at room temperature. The size of cryoloop 
should be carefully chosen so that the crystal does not move in the 
cryoloop. This crystal mounting method can be time-saving compared to 
the traditional method to mount a crystal in a glass capillary tube.



Many thanks

--
Dr. Cedric Bauvois
Cristallographie des protéines
Institut de Recherches Microbiologiques JM Wiame -IRMW
Av E. Gryzon 1, 1070 Brussels (Belgium)  
tél: +32 (0)2 5273634

fax: +32 (0)2 5267273

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-16 Thread Schubert, Carsten [PRDUS] 
http://www.mitegen.com/
 
Cheers

Carsten

-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk]on Behalf Of cedric 
bauvois
Sent: Friday, January 16, 2009 9:27 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cryoloops for X-ray data collection from protein crystals at 
room temperature


Dear CCP4ers,

in their paper entitled " Using cryoloops for X-ray data collection from 
protein crystals at room temperature: A simple applicable method" (  
<http://www.sciencedirect.com/science/journal/00220248> Journal of Crystal 
Growth
Volume  
<http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%235302%232005%23997189997%23601824%23FLA%23&_cdi=5302&_pubType=J&view=c&_auth=y&_acct=C26678&_version=1&_urlVersion=0&_userid=532047&md5=9a4e7b2fc158c6d2396925c79d995e3d>
 281, Issues 2-4, 1 August 2005, Pages 592-595.), the authors present a way to 
mount crystals using "a cryoloop accompanied by a glass capillary cap" (see 
abstract below).
Do you know if any commercial version of such system are now available ?


Abstract: Although cryoloops are now routinely used for X-ray data collection 
from protein crystals in cryocooling condition, it is still necessary to 
collect X-ray diffraction data from protein crystals at room temperature under 
such circumstances as to find resolution limit and/or to avoid damage of 
protein crystals at cryogenic temperature (e.g. 100 K). Here, we show that a 
cryoloop, which is accompanied by a glass capillary cap to maintain humid 
environment of crystal in the cryoloop, can be used not only to examine protein 
or non-protein crystals but also to collect X-ray diffraction data for 
structural analysis from protein crystals at room temperature. The size of 
cryoloop should be carefully chosen so that the crystal does not move in the 
cryoloop. This crystal mounting method can be time-saving compared to the 
traditional method to mount a crystal in a glass capillary tube.




Many thanks 


-- 
Dr. Cedric Bauvois
Cristallographie des protéines
Institut de Recherches Microbiologiques JM Wiame -IRMW
Av E. Gryzon 1, 1070 Brussels (Belgium)  
tél: +32 (0)2 5273634
fax: +32 (0)2 5267273

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-16 Thread Guenter Fritz 

Hi Cedric,
I used these, but just for testing crystals.  I was afraid that the 
crystal might move in the loop. For testing it worked pretty good.

http://www.jenabioscience.com/cms/en/1/catalog/733_microrttrade_room_temperature_mounting_system.html
There might be more suppliers. Please send a summary to ccp4bb.
Best
Guenter


cedric bauvois wrote:

Dear CCP4ers,

in their paper entitled " Using cryoloops for X-ray data collection 
from protein crystals at room temperature: A simple applicable method" 
( *Journal of Crystal Growth* 

Volume 281, Issues 2-4 
, 
1 August 2005, Pages 592-595.), the authors present a way to mount 
crystals using "a cryoloop accompanied by a glass capillary cap" (see 
abstract below).

Do you know if any commercial version of such system are now available ?

Abstract: Although cryoloops are now routinely used for X-ray data 
collection from protein crystals in cryocooling condition, it is still 
necessary to collect X-ray diffraction data from protein crystals at 
room temperature under such circumstances as to find resolution limit 
and/or to avoid damage of protein crystals at cryogenic temperature 
(e.g. 100 K). Here, we show that a cryoloop, which is accompanied by a 
glass capillary cap to maintain humid environment of crystal in the 
cryoloop, can be used not only to examine protein or non-protein 
crystals but also to collect X-ray diffraction data for structural 
analysis from protein crystals at room temperature. The size of 
cryoloop should be carefully chosen so that the crystal does not move 
in the cryoloop. This crystal mounting method can be time-saving 
compared to the traditional method to mount a crystal in a glass 
capillary tube.



Many thanks

--
Dr. Cedric Bauvois
Cristallographie des protéines
Institut de Recherches Microbiologiques JM Wiame -IRMW
Av E. Gryzon 1, 1070 Brussels (Belgium)  
tél: +32 (0)2 5273634

fax: +32 (0)2 5267273


--
***

Priv.Doz.Dr. Guenter Fritz
Fachbereich Biologie
Sektion Naturwissenschaften
Universitaet Konstanz
http://www.biologie.uni-konstanz.de/fritz

Universitaetsstrasse 10
Postfach M665
D-78457 Konstanz

e-mail: guenter.fr...@uni-konstanz.de

Tel. Office: +49-(0)7531 88 3205 
Tel. Lab   : +49-(0)7531 88 3687
Fax:  +49-(0)7531 88 2966

[ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-16 Thread cedric bauvois 
Dear CCP4ers,

in their paper entitled " Using cryoloops for X-ray data collection from
protein crystals at room temperature: A simple applicable method" ( *Journal
of Crystal Growth* 
 Volume 281, Issues
2-4,
1 August 2005, Pages 592-595.), the authors present a way to mount crystals
using "a cryoloop accompanied by a glass capillary cap" (see abstract
below).
Do you know if any commercial version of such system are now available ?

Abstract: Although cryoloops are now routinely used for X-ray data
collection from protein crystals in cryocooling condition, it is still
necessary to collect X-ray diffraction data from protein crystals at room
temperature under such circumstances as to find resolution limit and/or to
avoid damage of protein crystals at cryogenic temperature (e.g. 100 K).
Here, we show that a cryoloop, which is accompanied by a glass capillary cap
to maintain humid environment of crystal in the cryoloop, can be used not
only to examine protein or non-protein crystals but also to collect X-ray
diffraction data for structural analysis from protein crystals at room
temperature. The size of cryoloop should be carefully chosen so that the
crystal does not move in the cryoloop. This crystal mounting method can be
time-saving compared to the traditional method to mount a crystal in a glass
capillary tube.

Many thanks

-- 
Dr. Cedric Bauvois
Cristallographie des protéines
Institut de Recherches Microbiologiques JM Wiame -IRMW
Av E. Gryzon 1, 1070 Brussels (Belgium)
tél: +32 (0)2 5273634
fax: +32 (0)2 5267273