Re: [ccp4bb] first use of synchrotron radiation in PX
Dear James, I agree with your chronology of the first full new protein structures by SR MAD. The 1975 two wavelength Hoppe and Jakubowksi study of erythrocruorin with Ni and Co Kalpha Xray tubes is a classic piece of work of in effect MAD phasing . See the IUCr Anomalous Scattering Conference book edited by Abrahams and Ramaseshan. The 1971 Nature paper biological diffraction with SR from Hamburg, whose focus was on muscle diffraction, which Colin highlighted, does have an entry though for a 'protein crystal' in a table. There is also of course the Hamburg 1976 paper Harmsen et al J Mol Biol but which generally concluded SR for protein crystallography wasn't worth it; to me as a doctoral student at the time this was clearly an incorrect conclusion I firmly believed based on the 1971 Hamburg Nature paper and especially what I could see in and beyond the 1976 SSRL PNAS paper. Yours sincerely, John Prof John R Helliwell DSc On 16 Mar 2013, at 14:46, James Holton jmhol...@lbl.gov wrote: The first report of shooting a protein crystal at a synchrotron (I think) was in 1976: http://www.pnas.org/content/73/1/128.full.pdf that was rubredoxin The first PDB file that contains a SYNCHROTRON=Y entry is 1tld (trypsin), which was deposited in 1989: http://dx.doi.org/10.1016/0022-2836(89)90110-1 But the structure of trypsin was arguably already solved at that time. Anomalous diffraction was first demonstrated by Coster, Knoll and Prins in 1930 http://dx.doi.org/10.1007/BF01339610 this was 20 years before Bijvoet. But not with a synchrotron and definitely not with a protein The first protein to be solved using anomalous was crambin in 1981: http://dx.doi.org/10.1038/290107a0 but this was not using a synchrotron The first demonstration of MAD on a protein at a synchrotron was a Tb soak of parvalbumin in 1985 http://dx.doi.org/10.1016/0014-5793(85)80207-6 but one could argue that several parvalbumins were already known at that time. The first MAD structure from native metals was cucumber blue copper protein (2cbp) in 1989 http://dx.doi.org/10.1126%2Fscience.3406739 The first new structure using MAD, as well as the first SeMet was ribonuclease H (1rnh) in 1990 http://dx.doi.org/10.1126/science.2169648 If anyone knows of earlier cases, I'd like to hear about it! -James Holton MAD Scientist On 3/13/2013 7:38 AM, Alan Cheung wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan
Re: [ccp4bb] first use of synchrotron radiation in PX
The first report of shooting a protein crystal at a synchrotron (I think) was in 1976: http://www.pnas.org/content/73/1/128.full.pdf that was rubredoxin The first PDB file that contains a SYNCHROTRON=Y entry is 1tld (trypsin), which was deposited in 1989: http://dx.doi.org/10.1016/0022-2836(89)90110-1 But the structure of trypsin was arguably already solved at that time. Anomalous diffraction was first demonstrated by Coster, Knoll and Prins in 1930 http://dx.doi.org/10.1007/BF01339610 this was 20 years before Bijvoet. But not with a synchrotron and definitely not with a protein The first protein to be solved using anomalous was crambin in 1981: http://dx.doi.org/10.1038/290107a0 but this was not using a synchrotron The first demonstration of MAD on a protein at a synchrotron was a Tb soak of parvalbumin in 1985 http://dx.doi.org/10.1016/0014-5793(85)80207-6 but one could argue that several parvalbumins were already known at that time. The first MAD structure from native metals was cucumber blue copper protein (2cbp) in 1989 http://dx.doi.org/10.1126%2Fscience.3406739 The first new structure using MAD, as well as the first SeMet was ribonuclease H (1rnh) in 1990 http://dx.doi.org/10.1126/science.2169648 If anyone knows of earlier cases, I'd like to hear about it! -James Holton MAD Scientist On 3/13/2013 7:38 AM, Alan Cheung wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan
Re: [ccp4bb] first use of synchrotron radiation in PX
Thank you James, you should write a History book about the modern x-ray times. Or better make one of those movies you are famous for. Jürgen On Mar 16, 2013, at 10:46 AM, James Holton wrote: The first report of shooting a protein crystal at a synchrotron (I think) was in 1976: http://www.pnas.org/content/73/1/128.full.pdf that was rubredoxin The first PDB file that contains a SYNCHROTRON=Y entry is 1tld (trypsin), which was deposited in 1989: http://dx.doi.org/10.1016/0022-2836(89)90110-1 But the structure of trypsin was arguably already solved at that time. Anomalous diffraction was first demonstrated by Coster, Knoll and Prins in 1930 http://dx.doi.org/10.1007/BF01339610 this was 20 years before Bijvoet. But not with a synchrotron and definitely not with a protein The first protein to be solved using anomalous was crambin in 1981: http://dx.doi.org/10.1038/290107a0 but this was not using a synchrotron The first demonstration of MAD on a protein at a synchrotron was a Tb soak of parvalbumin in 1985 http://dx.doi.org/10.1016/0014-5793(85)80207-6 but one could argue that several parvalbumins were already known at that time. The first MAD structure from native metals was cucumber blue copper protein (2cbp) in 1989 http://dx.doi.org/10.1126%2Fscience.3406739 The first new structure using MAD, as well as the first SeMet was ribonuclease H (1rnh) in 1990 http://dx.doi.org/10.1126/science.2169648 If anyone knows of earlier cases, I'd like to hear about it! -James Holton MAD Scientist On 3/13/2013 7:38 AM, Alan Cheung wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
Re: [ccp4bb] first use of synchrotron radiation in PX
On Saturday, 16 March 2013, James Holton wrote: The first report of shooting a protein crystal at a synchrotron (I think) was in 1976: http://www.pnas.org/content/73/1/128.full.pdf that was rubredoxin The first PDB file that contains a SYNCHROTRON=Y entry is 1tld (trypsin), which was deposited in 1989: http://dx.doi.org/10.1016/0022-2836(89)90110-1 But the structure of trypsin was arguably already solved at that time. Anomalous diffraction was first demonstrated by Coster, Knoll and Prins in 1930 http://dx.doi.org/10.1007/BF01339610 this was 20 years before Bijvoet. But not with a synchrotron and definitely not with a protein The first protein to be solved using anomalous was crambin in 1981: http://dx.doi.org/10.1038/290107a0 but this was not using a synchrotron The first demonstration of MAD on a protein at a synchrotron was a Tb soak of parvalbumin in 1985 http://dx.doi.org/10.1016/0014-5793(85)80207-6 but one could argue that several parvalbumins were already known at that time. The first MAD structure from native metals was cucumber blue copper protein (2cbp) in 1989 http://dx.doi.org/10.1126%2Fscience.3406739 The original CBP MAD structure (1CBP) was published in 1988. Also 1988: Lamprey hemoglobin (Fe MAD) DOI: 10.1002/prot.340040202 1989: Streptavidin (Se MAD): PNAS 1989 86 (7) 2190-2194 The first new structure using MAD, as well as the first SeMet was ribonuclease H (1rnh) in 1990 http://dx.doi.org/10.1126/science.2169648 If anyone knows of earlier cases, I'd like to hear about it! Ethan -James Holton MAD Scientist On 3/13/2013 7:38 AM, Alan Cheung wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan
[ccp4bb] first use of synchrotron radiation in PX
Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: che...@lmb.uni-muenchen.de
Re: [ccp4bb] first use of synchrotron radiation in PX
Hi Not sure if this is strictly speaking the first protein *solved* on a synchrotron, but I think this is the first report of shooting protein crystals at a synchrotron in the widely available literature - http://www.pnas.org/content/73/1/128.full.pdf+html Phillips J C, Wlodawer A, Yevitz M M and Hodgson K 0 1976 Proc. Nat. Acad. Sci. USA 73 128-32 Applications of synchrotron radiation to protein crystallography: Preliminary results On 13 Mar 2013, at 14:38, Alan Cheung wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: che...@lmb.uni-muenchen.de Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing)
Re: [ccp4bb] first use of synchrotron radiation in PX
Did anyone see this prescient line in the PNAS paper? Seems that the MAD concept was suggested way back then... JPK While the enhancement of anomalous scattering has not yet been examined in detail, it is in principle possible to use data collected at three wavelengths (15) to completely solve the phase problem. The synchrotron source is uniquely suited for these applications. On Wed, Mar 13, 2013 at 11:04 AM, Harry Powell ha...@mrc-lmb.cam.ac.ukwrote: Hi Not sure if this is strictly speaking the first protein *solved* on a synchrotron, but I think this is the first report of shooting protein crystals at a synchrotron in the widely available literature - http://www.pnas.org/content/73/1/128.full.pdf+html Phillips J C, Wlodawer A, Yevitz M M and Hodgson K 0 1976 Proc. Nat. Acad. Sci. USA 73 128-32 Applications of synchrotron radiation to protein crystallography: Preliminary results On 13 Mar 2013, at 14:38, Alan Cheung wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: che...@lmb.uni-muenchen.de Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org ***
Re: [ccp4bb] first use of synchrotron radiation in PX
Nothing prescient about that. The MAD concept was first proposed by Herzberg and Lau in 1967, much before sycnhrotrons were used for protein crystallography. Herzenberg, A. Lau, H. S. M. (1967) Acta. Crystallogr. 22, 24-28. http://scripts.iucr.org/cgi-bin/paper?S0365110X6740 The PNAS paper even refers to this article. Ganesh Le 13/03/13 16:46, Jacob Keller a écrit : Did anyone see this prescient line in the PNAS paper? Seems that the MAD concept was suggested way back then... JPK While the enhancement of anomalous scattering has not yet been examined in detail, it is in principle possible to use data collected at three wavelengths (15) to completely solve the phase problem. The synchrotron source is uniquely suited for these applications.
Re: [ccp4bb] first use of synchrotron radiation in PX
Yes, this is a key paper demonstrating the possibilities. The answer to the question of first structure solved is a bit more difficult. Much of the early use of synchrotrons was for collecting high resolution data for refinement to supplement data collected on lab sources. This included data from similar structures with more or less sequence identity as well as data from heavy atom derivatives. MAD structures appeared somewhat later (see the references in Proc. Nati. Acad. Sci. USA Vol. 86, pp. 2190-2194, April 1989 for some early examples). Of course John Helliwell's book (Macromolecular Crystallography with Synchrotron Radiation, chapter 10) gives a useful historical introduction. Other than the above, if anyone has a claim to first structure solved just with synchrotron radiation then they should speak up! Colin -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Harry Powell Sent: 13 March 2013 15:04 To: ccp4bb Subject: Re: [ccp4bb] first use of synchrotron radiation in PX Hi Not sure if this is strictly speaking the first protein *solved* on a synchrotron, but I think this is the first report of shooting protein crystals at a synchrotron in the widely available literature - http://www.pnas.org/content/73/1/128.full.pdf+html Phillips J C, Wlodawer A, Yevitz M M and Hodgson K 0 1976 Proc. Nat. Acad. Sci. USA 73 128-32 Applications of synchrotron radiation to protein crystallography: Preliminary results On 13 Mar 2013, at 14:38, Alan Cheung wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: che...@lmb.uni-muenchen.de Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
Re: [ccp4bb] first use of synchrotron radiation in PX
When I started my PhD (in 1980!) at Imperial, David Blow already had a PhD student who's project was to use the new Daresbury synchrotron to exploit anomalous differences. Unfortunately it didn't come on line in time for him to actually get the data he needed. I'd be intrigued to know who got the first structure from Daresbury. I don't remember feeling there was a race at the time, but then we were a lot less competitive in those days! Peter On 13 March 2013 16:21, Colin Nave colin.n...@diamond.ac.uk wrote: Yes, this is a key paper demonstrating the possibilities. The answer to the question of first structure solved is a bit more difficult. Much of the early use of synchrotrons was for collecting high resolution data for refinement to supplement data collected on lab sources. This included data from similar structures with more or less sequence identity as well as data from heavy atom derivatives. MAD structures appeared somewhat later (see the references in Proc. Nati. Acad. Sci. USA Vol. 86, pp. 2190-2194, April 1989 for some early examples). Of course John Helliwell's book (Macromolecular Crystallography with Synchrotron Radiation, chapter 10) gives a useful historical introduction. Other than the above, if anyone has a claim to first structure solved just with synchrotron radiation then they should speak up! Colin -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Harry Powell Sent: 13 March 2013 15:04 To: ccp4bb Subject: Re: [ccp4bb] first use of synchrotron radiation in PX Hi Not sure if this is strictly speaking the first protein *solved* on a synchrotron, but I think this is the first report of shooting protein crystals at a synchrotron in the widely available literature - http://www.pnas.org/content/73/1/128.full.pdf+html Phillips J C, Wlodawer A, Yevitz M M and Hodgson K 0 1976 Proc. Nat. Acad. Sci. USA 73 128-32 Applications of synchrotron radiation to protein crystallography: Preliminary results On 13 Mar 2013, at 14:38, Alan Cheung wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: che...@lmb.uni-muenchen.de Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
Re: [ccp4bb] first use of synchrotron radiation in PX
Dear Colleagues, The paper http://dx.doi.org/10.1107/S0108768185002233 in work led by Howard Einspahr undertaken at SRS 7.2 is a protein structural specific result from synchrotron radiation. The MAD method of course yielded totally specific to SR protein crystal structures. The conceptualisation goes further back than Herzenberg and Lau namely to Okaya and Pepinsky 1956. The seleno met approach of Wayne Hendrickson I believe was however the major breakthrough. Best wishes, John Prof John R Helliwell DSc FInstP CPhys FRSC CChem F Soc Biol. Chair School of Chemistry, University of Manchester, Athena Swan Team. http://www.chemistry.manchester.ac.uk/aboutus/athena/index.html On 13 Mar 2013, at 14:38, Alan Cheung che...@lmb.uni-muenchen.de wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: che...@lmb.uni-muenchen.de
Re: [ccp4bb] first use of synchrotron radiation in PX
Sivaraj Ramesechan was outlining the physics of multiple wavelength anom scattering in the 1960s as a method for solving insulin. It was purely theoretical then; no instruments to make the measurements.. Eleanor On 13 Mar 2013, at 17:19, Peter Moody wrote: When I started my PhD (in 1980!) at Imperial, David Blow already had a PhD student who's project was to use the new Daresbury synchrotron to exploit anomalous differences. Unfortunately it didn't come on line in time for him to actually get the data he needed. I'd be intrigued to know who got the first structure from Daresbury. I don't remember feeling there was a race at the time, but then we were a lot less competitive in those days! Peter On 13 March 2013 16:21, Colin Nave colin.n...@diamond.ac.uk wrote: Yes, this is a key paper demonstrating the possibilities. The answer to the question of first structure solved is a bit more difficult. Much of the early use of synchrotrons was for collecting high resolution data for refinement to supplement data collected on lab sources. This included data from similar structures with more or less sequence identity as well as data from heavy atom derivatives. MAD structures appeared somewhat later (see the references in Proc. Nati. Acad. Sci. USA Vol. 86, pp. 2190-2194, April 1989 for some early examples). Of course John Helliwell's book (Macromolecular Crystallography with Synchrotron Radiation, chapter 10) gives a useful historical introduction. Other than the above, if anyone has a claim to first structure solved just with synchrotron radiation then they should speak up! Colin -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Harry Powell Sent: 13 March 2013 15:04 To: ccp4bb Subject: Re: [ccp4bb] first use of synchrotron radiation in PX Hi Not sure if this is strictly speaking the first protein *solved* on a synchrotron, but I think this is the first report of shooting protein crystals at a synchrotron in the widely available literature - http://www.pnas.org/content/73/1/128.full.pdf+html Phillips J C, Wlodawer A, Yevitz M M and Hodgson K 0 1976 Proc. Nat. Acad. Sci. USA 73 128-32 Applications of synchrotron radiation to protein crystallography: Preliminary results On 13 Mar 2013, at 14:38, Alan Cheung wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: che...@lmb.uni-muenchen.de Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
Re: [ccp4bb] first use of synchrotron radiation in PX
Jean Witz (now deceased) once told me that the following paper is the first one mentionning data collection on a synchrotron. The journal is not really obscure and the paper should easily be found. The work was done in Germany, if I remember well. G. Rosenbaum, K.C. Holmes and J. Witz, Synchrotron radiation as a source for X-ray diffraction, Nature, 230, 434-437 (1971). Philippe Dumas
Re: [ccp4bb] first use of synchrotron radiation in PX
And indeed this experiment was done properly ... in a suit and tie! http://www.embl-hamburg.de/aboutus/general_information/HH_about/history/HH-holmes.jpg A. PS The journal is indeed a bit obscure ... On 13 Mar 2013, at 20:22, DUMAS Philippe (UDS) wrote: Jean Witz (now deceased) once told me that the following paper is the first one mentionning data collection on a synchrotron. The journal is not really obscure and the paper should easily be found. The work was done in Germany, if I remember well. G. Rosenbaum, K.C. Holmes and J. Witz, Synchrotron radiation as a source for X-ray diffraction, Nature, 230, 434-437 (1971). Philippe Dumas
Re: [ccp4bb] first use of synchrotron radiation in PX
Nice account on the subject J Synchrotron Radiat. 2010 July 1; 17(Pt 4): 433–444. Published online 2010 May 14. doi: 10.1107/S0909049510011611 Impact of synchrotron radiation on macromolecular crystallography: a personal view Zbigniew Dauter,a,* Mariusz Jaskolski,b,* and Alexander Wlodawer,c,* On 13 Mar 2013, at 19:22, DUMAS Philippe (UDS) p.du...@ibmc-cnrs.unistra.fr wrote: Jean Witz (now deceased) once told me that the following paper is the first one mentionning data collection on a synchrotron. The journal is not really obscure and the paper should easily be found. The work was done in Germany, if I remember well. G. Rosenbaum, K.C. Holmes and J. Witz, Synchrotron radiation as a source for X-ray diffraction, Nature, 230, 434-437 (1971). Philippe Dumas Roberto A. Steiner Group Leader Randall Division of Cell and Molecular Biophysics King's College London roberto.stei...@kcl.ac.uk Room 3.10A New Hunt's House Guy's Campus SE1 1UL London Phone 0044 20 78488216 Fax0044 20 78486435
Re: [ccp4bb] first use of synchrotron radiation in PX
I never would have survived the dress code back then. From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Anastassis Perrakis [a.perra...@nki.nl] Sent: Wednesday, March 13, 2013 2:27 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] first use of synchrotron radiation in PX And indeed this experiment was done properly ... in a suit and tie! http://www.embl-hamburg.de/aboutus/general_information/HH_about/history/HH-holmes.jpg A. PS The journal is indeed a bit obscure ... On 13 Mar 2013, at 20:22, DUMAS Philippe (UDS) wrote: Jean Witz (now deceased) once told me that the following paper is the first one mentionning data collection on a synchrotron. The journal is not really obscure and the paper should easily be found. The work was done in Germany, if I remember well. G. Rosenbaum, K.C. Holmes and J. Witz, Synchrotron radiation as a source for X-ray diffraction, Nature, 230, 434-437 (1971). Philippe Dumas
Re: [ccp4bb] first use of synchrotron radiation in PX
The work at Hamburg was on insect flight muscle. It is usually quoted as the first x-ray diffraction using a synchrotron. The work is acknowledged as pioneering in John Helliwell's book. Of course the first data collection on a synchrotron was much earlier. A good account can be found here http://xdb.lbl.gov/Section2/Sec_2-2.html Colin -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of DUMAS Philippe (UDS) Sent: 13 March 2013 19:22 To: ccp4bb Subject: Re: [ccp4bb] first use of synchrotron radiation in PX Jean Witz (now deceased) once told me that the following paper is the first one mentionning data collection on a synchrotron. The journal is not really obscure and the paper should easily be found. The work was done in Germany, if I remember well. G. Rosenbaum, K.C. Holmes and J. Witz, Synchrotron radiation as a source for X-ray diffraction, Philippe Dumas
