Re: [ccp4bb] Questions about antibody in crystallization

[Jarrod Mousa]

Yes, the hinge region between Fab and Fc is highly flexible. If you look at
IgG by EM you will see many different conformations. Best to use Fab or
scFv.

Jarrod Mousa

On Thu, Jun 22, 2017 at 1:39 PM, Cheng Zhang  wrote:

> Hi all,
>
> I have a naive question about antibodies. Many people used Fab fragments
> in crystallization. I am wondering if it is possible to use the whole IgG
> molecule with Fc fragment as well. Or it would be too flexible and bad for
> crystallization?
>
> Thanks,
>
> Cheng
>
>
> --
> -
> Cheng Zhang
>

Re: [ccp4bb] Questions about antibody in crystallization

[Gert Vriend]

The fact that the PDB holds hundreds of FABs and a handful whole ABs 
suggests to me that the latter are hard to get crystals for. So, all the 
more reason for us bioinformaticians that you, experimentalists try it :-)



Gert


On 22-6-2017 20:39, Cheng Zhang wrote:

Hi all,

I have a naive question about antibodies. Many people used Fab 
fragments in crystallization. I am wondering if it is possible to use 
the whole IgG molecule with Fc fragment as well. Or it would be too 
flexible and bad for crystallization?


Thanks,

Cheng


--
-
Cheng Zhang