[Freesurfer] Amygdala subfields

2014-11-24 Thread stdp82
Dear all,
is the amygdala subfields segmentation available or you are working on it?
Thanks,

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Re: [Freesurfer] Amygdala subfields

2014-11-24 Thread Eugenio Iglesias
Hi Stefano,
we are working on an algorithm to segment the nuclei, but unfortunately I do 
not think it is going to be released any time soon.
Kind regards,
/Eugenio

Juan Eugenio Iglesias
Postdoctoral researcher BCBL
www.jeiglesias.com
www.bcbl.eu

Legal disclaimer/Aviso legal/Lege-oharra: www.bcbl.eu/legal-disclaimer


- Original Message -
From: std...@virgilio.it
To: freesurfer@nmr.mgh.harvard.edu
Sent: Monday, November 24, 2014 3:42:05 AM
Subject: [Freesurfer] Amygdala subfields


Dear all, 


is the amygdala subfields segmentation available or you are working on it? 


Thanks, 




Stefano 
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[Freesurfer] Creat ROI from annotation file

2014-11-24 Thread wangzhiwei3233
Hi Freesurfer experts,
I want creat some ROI masks or parcellation from Caret 
PALS_B12_Visuotopic.annot file in fsaverage's label folder.

I used the command mri_label2vol,
mri_label2vol --annot $Dir/label/lh.PALS_B12_Visuotopic.annot --temp 
$Dir/mri/T1.nii --fillthresh .5 --o PALS_B12_Visuotopic.nii --subject fsaverage 
--hemi lh --identity 
The parcellation in output volume were like some lines located at the boundry 
of white matter , and did not cover the gray matter (see picture).


Then, I used another methods as follows.
Firstly, I converted the annotation file into some label files using 
mri_annotation2label command;
Secondly, I created ROIs using these labels by mri_vol2roi.
However, the masks also couldn't cover the gray matter.


Could anyone help me creat good masks?
Thanks a lot!


Zhiwei 







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Re: [Freesurfer] Regarding recon-all

2014-11-24 Thread Bruce Fischl

sorry, I think =5 is in brain

On Mon, 24 Nov 2014, Gunjan Gautam wrote:


Hi Bruce,
What about the voxels having value equal to 5 (if exists) ?

Gunjan   

On Sun, Nov 23, 2014 at 10:39 PM, Gunjan Gautam gunjan.gemin...@gmail.com
wrote:

  Ok thank you Bruce

  On Nov 23, 2014 10:37 PM, Bruce Fischl
  fis...@nmr.mgh.harvard.edu wrote:
we don't have a binary one, but all voxels in
brainmask.mgz that are 5 are in brain and
everything 5 is not in brain

cheers
Bruce

On Sun, 23 Nov 2014, Gunjan Gautam wrote:


  Thanks,

  And what about the binary brain mask? I
  am able to see only the stripped
  volume but need the binary brain mask as
  well.

  Gunjan

  On Nov 23, 2014 8:49 PM, Bruce Fischl
  fis...@nmr.mgh.harvard.edu wrote:
        Hi Gunjan

        15 min is about what it is going
  to take. You could do it faster
        but then the failure rate would go
  up. We've tuned it to improve
        automaticity and reduce failures
  quite a bit.

        cheers
        Bruce


        On Sat, 22 Nov 2014, Gunjan Gautam
  wrote:


              Hi,

              I used recon-all with all
  and its taking a lot of
              time. My main concern is
              only to get the stripped
  brain.
              I tried with autorecon1 as
  well but its also taking
              not less than 15min and
              I need to deal with a huge
  data. 
              Last stage of autorecon1 is
  skull stripping which
              takes the NU corrected
              input (as it FS works as a
  pipeline). Is there any
              way to directly get the
              stripped brain and its
  binary mask.

              I also need to ask, which
  file of the output (name
              with extension) is the
              binary brain mask ?

              Best regards,
              Gunjan



       
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Re: [Freesurfer] Tracula_critical issue

2014-11-24 Thread Alshikho, Mohamad J.
Dear Anastasia,
Thank you very much for your answer. 
In my analysis I have two groups 23 patients and 27 controls. My problem right 
now is that this change in the results (from the output of trac-all -path -c)  
gave me different P values between the groups, sometimes the results are 
significant and sometimes it is not! Till today I repeated this analysis many 
times and I collected the results after 5 consecutive runs (all the tracts for 
all the subjects)

Recently and In order to find a solution for this problem I used the following 
command line to generate the mean metrics and the tracts volume:

fslstats - dti_FA.nii.gz -k path.pd_bin.nii.gz -M -V   [ I binarized 
path.pd.nii.gz (the output of trac-all -path -c for every tract) using 
fslmaths path.pd.nii.gz -thr 0.999 path.pd_bin.nii.gz and I used this treashold 
to make my mask conservative as possible] 

In this case I got stable numbers. i.e when I run this command line again the 
results are the same.
Kindly is this valid? can I use fslstats instead of the third step in Tracula 
trac-all -path -c to calculate the mean metrics and the tract's volume?

Also what is the difference between fslstats and trac-all -path -c (technically)

I highly appreciate your support

Mohamad

From: freesurfer-boun...@nmr.mgh.harvard.edu 
[freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Anastasia Yendiki 
[ayend...@nmr.mgh.harvard.edu]
Sent: Friday, November 21, 2014 5:10 PM
To: Freesurfer support list
Subject: Re: [Freesurfer] Tracula_critical issue

Hi Mohamad - As it's a probabilistic method that's sampling from a
distribution, there is a bit of randomness built in (which should decrease
as you increase the number of samples that you collect). You'll find that
the the weighted average measures are the most reliable, as they are less
affected by the tails of the distribution.

a.y

On Wed, 5 Nov 2014, Alshikho, Mohamad J. wrote:

 Hi Anastasia,
 I started recently using Tracula to do tractography for my DTI data. I did 
 the analysis exactly as mentioned
 in wiki. The problem is that when I am repeating the third step (trac-all 
 -path -c ) for the same subjects to
 generate the statistics; Tracula is updating all the information mentioned in 
 the file pathstats.overal.txt
 regarding the tract's volume and the tract's metrics values.
 Every time the output is different. I think we have a problem in the scripts 
 or something like this?

 Attached are three pathstats.overal.txt files for the same subject after 
 three consecutive runs for the
 trac-all -path -c (the same subject)


 Looking forward for your support
 Thanks



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Re: [Freesurfer] tksurfer curvature and overlay displays

2014-11-24 Thread Bruce Fischl
Hi Graham

have you tried freeview? We stopped development on the tktools some time 
ago.

cheers
Bruce

On Fri, 21 Nov 2014, Graham Wideman wrote:

 Hi all,

 I'm having some issues (some possibly mine, some possibly tksurfer's) getting 
 tksurfer (5.3c6) to display curvature the way I think it should be able to.  
 I am using the bert sample data.

 The Curvature display
 -
 If I use the Show Curvature button in the UI control panel, I get the 
 familiar gyrus=green; sulcus=red pattern applied to the displayed brain.

 The Configure Curvature Display is a bit of a shambles, and I think should be 
 fixed. First, it is virtually impossible to set midpoint using the slider, 
 because the values it pushes to the adjacent textbox are way too coarse. 
 Typing values into the midpoint textbox works, in that it affects the balance 
 of red and green in the display.  However the textbox does not affect the 
 position of the slider, suggesting that it actually does not work.

 That said, there's no actual adjustment of the threshold size. Indeed, it's 
 not clear that there actually IS a threshold -- perhaps there isn't. Maybe 
 threshold midpoint just sets the midpoint of the color range?

 And then there's a a textbox labeled threshold slope. I'm pretty sure 
 that's nonsensical phrase. What this box appears to do is set the ratio of 
 color to curvature value. Ie: Color intensity.

 Bottom line -- this dialog only partially fits the purpose at hand, and is so 
 confusingly wording that ones attempts to use it are pretty much random. It 
 may even be that some UI elements or functionality that was once there has 
 gone missing accidentally?

 Finally, I wanted a colorbar for the curvature display, and so far as I can 
 tell there isn't one. I can turn on a colorbar, but it's the one for overlays.

 Using a Curvature variable as an overlay
 
 I next tried to read in the k1.crv variable as an overlay. This sort of 
 works, but gives a very mottled display (except using heat map color 
 scheme), I think because even in definite sulcus and gyrus regions, the 
 curvature values straddle the border of the grey range of color, and I'm not 
 sure how to adjust it sensibly.

 -- I couldn't figure out how to set the overall color range, that is to say 
 the extremes of the colorbar. These seem stubbornly stuck at 5.0 and -5.0. In 
 particular, the Theshhold Max setting doesn't seem to affect the extremes of 
 the color range.

 -- I couldn't figure out how to eliminate the large grey range in the middle 
 of the color range. I noted that the Threshhold Min setting can be used to 
 make the grey range larger, but setting Min below 1.5 (for example, zero) 
 does not eliminate the grey between 0 and +/-1.5.

 

 Anybody have any helpful clues on these items!

 Thanks!

 -- Graham


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Re: [Freesurfer] prefrontal cortex label

2014-11-24 Thread Douglas N Greve
What is the full terminal output and error msg?

On 11/21/2014 04:14 AM, Anna Jonsson wrote:
 hi, I have tried to make prefrotnal cortex label by doing first



 mri_annotation2label --subject $subject --hemi rh --outdir 
 $subject/label



 mri_mergelabels -i lh.caudalanteriorcingulate.label -i 
 lh.caudalmiddlefrontal.label -i lh.lateralorbitofrontal.label -i 
 lh.medialorbitofrontal.label -i lh.parsopercularis.label -i 
 lh.parsorbitalis.label -i lh.parstriangularis.label -i 
 lh.rostralanteriorcingulate.label -i lh.rostralmiddlefrontal.label -i 
 lh.superiorfrontal.label -i lh.lateralorbitofrontal.label -o 
 lh.prefrontalcortex.label -d /directory/subject/label


 which does create a label called x.prefrontalcortex.label in each 
 persons directory. then however when i try to do

 mris_anatomical_stats -l 
 $SUBJECTS_DIR/$subject/label/lh.prefrontalcortex.label $subject lh 
 pial | grep thickness  left_prefrontal

 i get error and say it cant find label. any pointers on what im doing 
 wrong?

 thank you





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Re: [Freesurfer] mri_glmfit-sim sim-sign

2014-11-24 Thread Douglas N Greve
Have you looked at
  mri_glmfit-sim --help


On 11/21/2014 01:51 PM, Jon Alan Wieser wrote:


 hi

 please explain the differece between --sim-sign   abs, neg, and pos



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Re: [Freesurfer] seed-based fcMRI analysis between thalamus and cortex

2014-11-24 Thread Douglas N Greve

I have not read that paper, but I think it can do what you want. Have 
you seen?
http://surfer.nmr.mgh.harvard.edu/fswiki/FsFastFunctionalConnectivityWalkthrough

On 11/22/2014 11:55 AM, std...@virgilio.it wrote:
 Dear all,

 is FS-FAST able to perform seed-based fcMRI analysis between thalamus 
 and cortex (e.g. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852505/)?

 Thanks for your work,


 Stefano


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Re: [Freesurfer] Resample the surface mesh

2014-11-24 Thread Douglas N Greve
Would mris_decimate work?

On 11/24/2014 01:53 AM, 婷 wrote:
 Thanks for your reply with detailed explanation. I am interested in what if 
 we apply analysis on native surface first. Still wondering:)

 Ting


 On Nov 23, 2014, at 8:23 PM, Thomas Yeo ytho...@csail.mit.edu wrote:

 Hi Ting,

 What I usually do is to project my functional data to fsaverage space
 and then smooth and downsample to whatever resolution I want. There
 are different fsaverage resolution meshes, like fsaverage5 (~20k
 vertices) and fsaverage6 (~80k vertices). This can be achieved using
 standard freesurfer tools like mri_vol2surf and mri_surf2surf. Note
 that in this scenario, even though the data is in fsaverage space,
 this does not preclude individual subject analysis. The advantage of
 the above approach is that we can still easily perform a group
 analysis if we want to in the future.

 The disadvantage is that we lose a little bit of the subject's native
 cortical geometry, which is not an issue for your proposed approach.
 However, I am not aware of freesurfer tools that will let you
 downsample an arbitrary mesh. Perhaps someone who is more intimately
 aware of freesurfer's tools can jump in on this.

 Cheers,
 Thomas

 On Mon, Nov 24, 2014 at 5:10 AM, ting xu xutin...@gmail.com wrote:
 Hi Thomas,

 I want to analyze the data in native surface space. The functional volume
 data could be register to native surface with bbregister, however, the
 resolution of the native surface is quite high, too much nodes for
 computational capacity to me. Any ideas?

 Thanks,

 Ting


 On Fri, Nov 21, 2014 at 10:37 PM, Thomas Yeo ytho...@csail.mit.edu wrote:

 Hi Ting,

 I am not an expert, but why do you want to downsample the native
 surface mesh? The usual way of doing this is to register the full
 surface mesh with the functional volume data using Doug's bbregister.

 Cheers,
 Thomas

 On Fri, Nov 21, 2014 at 4:08 AM, ting xu xutin...@gmail.com wrote:
 Dear freesurfer experts,

 Is there any way to down resample the native surface mesh and register
 the
 functional volume data to the down resampled surface in freesurfer?

 Many thanks,

 Ting


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Re: [Freesurfer] Creat ROI from annotation file

2014-11-24 Thread Douglas N Greve

Try using mri_aparc2aseg. This is what creates aparc+aseg.mgz from the 
aparc annot. You can look in the recon-all.log file for the command line 
(just make sure to change the output file name too so that you don't 
overwrite aparc+aseg.mgz)

doug

On 11/24/2014 07:54 AM, wangzhiwei3233 wrote:
 Hi Freesurfer experts,
 I want creat some ROI masks or parcellation from Caret 
 PALS_B12_Visuotopic.annot file in fsaverage's label folder.

 I used the command mri_label2vol,
 mri_label2vol --annot $Dir/label/lh.PALS_B12_Visuotopic.annot --temp 
 $Dir/mri/T1.nii --fillthresh .5 --o PALS_B12_Visuotopic.nii --subject 
 fsaverage --hemi lh --identity
 The parcellation in output volume were like some lines located at the 
 boundry of white matter , and did not cover the gray matter (see picture).

 Then, I used another methods as follows.
 Firstly, I converted the annotation file into some label files using 
 mri_annotation2label command;
 Secondly, I created ROIs using these labels by mri_vol2roi.
 However, the masks also couldn't cover the gray matter.

 Could anyone help me creat good masks?
 Thanks a lot!

 Zhiwei








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Re: [Freesurfer] Group analysis

2014-11-24 Thread Douglas N Greve

There are two things that are considered. One is the tesselation which 
smoothing is one. The other is the registration that is used. Using 
-target Something only specifies the smoothing target (if doing 
smoothing in mris_preproc). If you want to spec a registration target, 
you need to first run make_average_subject (which looks like you have 
done). Second, you need to run surfreg for each subject specifying your 
average subject with --t. This will create ?h.MyAverage.sphere.reg in 
each subject's surf folder. Then run mris_preproc with --surfre 
MyAverage.sphere.reg

doug

On 11/13/2014 05:06 AM, Roberta Santoro wrote:

 Dear all,

 I created an average subject from my dataset and I would like to use 
 it to run a group analysis of thickness. I am using the command:

 recon-all -s subjid -qcache -target MyAverage

 When looking at the terminal, I noticed that the srcsurfreg and the 
 trgsurfreg are both sphere.reg: shouldn’t one of the two be 
 sphere.reg.MyAverage? Otherwise, how is the alignment achieved?

 Thanks for your help.

 Best,

 Roberta



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Re: [Freesurfer] multiple comparisons correction for individual hemispheres (native space)

2014-11-24 Thread Douglas N Greve
Hi Christopher, sorry for the delay. I had a write a new program. You 
can download it from here
ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/cluster-sess
Let me know how it goes

doug

On 11/14/2014 06:05 AM, Milde, Christopher wrote:
 Dear Freesurfer experts,

 I performed fMRI analysis using FSFast resulting in uncorrected significance 
 maps (fsig.nii.gz). For selxavg3-sess I did not use the -fwhm flag (I rerun 
 selxavg3-sess with -fwhm at moment).

 How can I use simulations for native space hemispheres?. I tried to adapt 
 mri_glmfit-sim but unfortunately it failed...

 Sincerely yours,

 Christopher

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[Freesurfer] pial mask

2014-11-24 Thread Octavian Lie
Dear all,

After running the recon-all on a subject, I needed to create a 'pial'
surface to include the hippocampi, which are not included well in the pial
reconstruction. So I generated a mask and used it to mask brain.mgz.

1. All well, except that in both the mask and the masked brain volume
(readybrain.mgz, see below), the fill extends with a variable layer of
about 1 voxel) above the pial red contour in some areas (see the top,
leading me to believe that the red contours we use when editing are not the
actual pial surfaces, but some interpolations of the actual calculated pial
surface. If this is true, then there may be some editing errors when using
these contours. Another explanation could be the resolution of the
mris_fill.
Please let me know which is the case, and how to fix it so that the mask
closely follows the red contour.

2. I need to generate a surface from readybrain.mgz, preferably that can be
registered to the sphere.reg and on which cortical parcellation (DK atlass)
can be applied. Is there a way? If not, I would still like to know how to
generate the surface of this volume.

Thank you,

Octavian

Here is the code I used:

cd /usr/local/fs5.3/freesurfer/subjects/subjid

mris_fill -c -r 0.1 surf/rh.pial mri/rh.pial.filled.mgz  \
mris_fill -c -r 0.1 surf/lh.pial mri/lh.pial.filled.mgz  \
mri_concat --combine --i mri/rh.pial.filled.mgz --i mri/lh.pial.filled.mgz
--o mri/pial.filled.mgz  \
cd ./mri  \
mri_binarize --i aseg.mgz --match 5 --match 9 --match 10 --match 14 --match
17 --match 18 --match 19\
 --match 25 --match 31 --match 44 --match 48 --match 49 --match 53 --match
54 --match 55 --match 57\
 --match 63 --match 86 --match 96 --match 97 --match 105 --match 106
--match 114 --match 115\
 --match 195 --match 199 --match 203 --match 204 --match 205 --match 216
--match 222 --match 225\
 --match 507 --match 557 --match 1200 --match 1201 --match 1205 --match
1206 --match 2200 --match 2201\
 --match 2205 --match 2206 --o hippoamy.mask.mgz  \
fscalc pial.filled.mgz add hippoamy.mask.mgz -o epimask.mgz  \
mri_convert brain.mgz brain.float.mgz -odt float  \
mri_mask brain.float.mgz epimask.mgz readybrain.mgz
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Re: [Freesurfer] can I apply freesurfer significant clusters as ROI in fMRI study?

2014-11-24 Thread Douglas N Greve

What do you mean apply it to your functional data? Analyzed fMRI? In 
what package? In what space is the fMRI?

On 11/20/2014 08:01 AM, chenchunhuichina wrote:
 Dear freesurfer experts,
 I searched on the wiki but can not figure out how to solve this 
 problem: I found some significant clusters on graymatter 
 thickness, how can I use it as a ROI in my functional MRI study?
 I have both structure and function data of the same subjects, so I can 
 either do it in stardard space or native space one by one if possible.
 Any help is appriciated~
 Best
 2014-11-20
 
 Chunhui Chen
 _
 State Key Laboratory of Cognitive Neuroscience and Learning
 Beijing Normal University
 Beijing, China 100875


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[Freesurfer] Download of latest Tracula version not possible

2014-11-24 Thread Janosch Linkersdörfer
Hi all,

I am trying to download the latest version of Tracula from this site 
http://surfer.nmr.mgh.harvard.edu/fswiki/Tracula. 

This seems not to be possible at the moment. Are there server problems?

Thanks,

Janosch
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Re: [Freesurfer] Creat ROI from annotation file

2014-11-24 Thread wangzhiwei3233
 It works! Thanks, Doug.

At 2014-11-25 01:19:13, Douglas N Greve gr...@nmr.mgh.harvard.edu wrote:

Try using mri_aparc2aseg. This is what creates aparc+aseg.mgz from the 
aparc annot. You can look in the recon-all.log file for the command line 
(just make sure to change the output file name too so that you don't 
overwrite aparc+aseg.mgz)

doug

On 11/24/2014 07:54 AM, wangzhiwei3233 wrote:
 Hi Freesurfer experts,
 I want creat some ROI masks or parcellation from Caret 
 PALS_B12_Visuotopic.annot file in fsaverage's label folder.

 I used the command mri_label2vol,
 mri_label2vol --annot $Dir/label/lh.PALS_B12_Visuotopic.annot --temp 
 $Dir/mri/T1.nii --fillthresh .5 --o PALS_B12_Visuotopic.nii --subject 
 fsaverage --hemi lh --identity
 The parcellation in output volume were like some lines located at the 
 boundry of white matter , and did not cover the gray matter (see picture).

 Then, I used another methods as follows.
 Firstly, I converted the annotation file into some label files using 
 mri_annotation2label command;
 Secondly, I created ROIs using these labels by mri_vol2roi.
 However, the masks also couldn't cover the gray matter.

 Could anyone help me creat good masks?
 Thanks a lot!

 Zhiwei








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Re: [Freesurfer] Resample the surface mesh

2014-11-24 Thread
It works! Thank you, Doug.

Ting

 On Nov 24, 2014, at 12:17 PM, Douglas N Greve gr...@nmr.mgh.harvard.edu 
 wrote:
 
 Would mris_decimate work?
 
 On 11/24/2014 01:53 AM, 婷 wrote:
 Thanks for your reply with detailed explanation. I am interested in what if 
 we apply analysis on native surface first. Still wondering:)
 
 Ting
 
 
 On Nov 23, 2014, at 8:23 PM, Thomas Yeo ytho...@csail.mit.edu wrote:
 
 Hi Ting,
 
 What I usually do is to project my functional data to fsaverage space
 and then smooth and downsample to whatever resolution I want. There
 are different fsaverage resolution meshes, like fsaverage5 (~20k
 vertices) and fsaverage6 (~80k vertices). This can be achieved using
 standard freesurfer tools like mri_vol2surf and mri_surf2surf. Note
 that in this scenario, even though the data is in fsaverage space,
 this does not preclude individual subject analysis. The advantage of
 the above approach is that we can still easily perform a group
 analysis if we want to in the future.
 
 The disadvantage is that we lose a little bit of the subject's native
 cortical geometry, which is not an issue for your proposed approach.
 However, I am not aware of freesurfer tools that will let you
 downsample an arbitrary mesh. Perhaps someone who is more intimately
 aware of freesurfer's tools can jump in on this.
 
 Cheers,
 Thomas
 
 On Mon, Nov 24, 2014 at 5:10 AM, ting xu xutin...@gmail.com wrote:
 Hi Thomas,
 
 I want to analyze the data in native surface space. The functional volume
 data could be register to native surface with bbregister, however, the
 resolution of the native surface is quite high, too much nodes for
 computational capacity to me. Any ideas?
 
 Thanks,
 
 Ting
 
 
 On Fri, Nov 21, 2014 at 10:37 PM, Thomas Yeo ytho...@csail.mit.edu 
 wrote:
 
 Hi Ting,
 
 I am not an expert, but why do you want to downsample the native
 surface mesh? The usual way of doing this is to register the full
 surface mesh with the functional volume data using Doug's bbregister.
 
 Cheers,
 Thomas
 
 On Fri, Nov 21, 2014 at 4:08 AM, ting xu xutin...@gmail.com wrote:
 Dear freesurfer experts,
 
 Is there any way to down resample the native surface mesh and register
 the
 functional volume data to the down resampled surface in freesurfer?
 
 Many thanks,
 
 Ting
 
 
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[Freesurfer] Tracula mri_convert error

2014-11-24 Thread Janosch Linkersdörfer
Hi experts,

I wanted to play with Tracula. Strangely, already the first preprocessing step, 
i.e., conversion to .nii.gz, fails.

Apparently, mri_convert (falsely) recognizes my dicom files as MGH dicoms 
(This looks like an MGH DTI volume). Could this be the reason why it fails?

Thanks,

Janosch







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