[Histonet] Uneven H & E Stain
We had the same problem. The xylene had nothing to do with it and thick and thin sections was one thing that we thought might be the problem. But when I destained and restained the same slides they stained the way they were supposed to . Finally we found that we had a problem with the wash stations of our sakura stainer. We had someone from sakaura come out and work on the machine and the staining has returned to what it is supposed to be. It seems when we have too many racks on the stainer this problem comes back intermitently, and sometimes we have to shut the machine down and turn it back on. Not sure exactly why this happens but if we follow this course of action our slides are staining correctly. Connie Grubaugh Las Vegas Nv. _ See how Windows® connects the people, information, and fun that are part of your life http://clk.atdmt.com/MRT/go/119463819/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Uneven H&E Staining (Karla Arrington)
One other possiblity to consider is a thinner section at the bottom. That could be the answer if the problem is intermittent even within the same staining rack. If the section is thinner, the staining appears paler. Good luck discovering the cause! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 60, Issue 32
Histonet, anybody know where I can find Sheep Liver, we have a research person looking for a small piece or a block. Thanks in advance. Rudy [EMAIL PROTECTED] Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PSlim Slide Printer
Hello Everyone, I have been following Victor's updates on the PSlim slide printer the past couple of months. Who else is using this device out there? I have some questions about it. What kind of slides are you using? Have you had any issues with it? Thanks in advance, Tyler Liebig [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Dako
I am sorry---it is 5 trays of 10 including the RUSH tray Roxanne -Original Message- From: [EMAIL PROTECTED] To: [EMAIL PROTECTED]; [EMAIL PROTECTED]; Histonet@lists.utsouthwestern.edu Sent: Wed, 19 Nov 2008 5:17 pm Subject: Re: [Histonet] Dako Just another FYI... The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC you do either of these machines would work out great. Roxanne -Original Message- From: Josie Britton <[EMAIL PROTECTED]> To: Michael Bradley <[EMAIL PROTECTED]>; Histonet@lists.utsouthwestern.edu Sent: Tue, 18 Nov 2008 11:31 am Subject: RE: [Histonet] Dako Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Dako
I didn't think that it had deparaffinization and retrieval on line. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Behalf Of [EMAIL PROTECTED] Sent: Wednesday, November 19, 2008 5:18 PM To: [EMAIL PROTECTED]; [EMAIL PROTECTED]; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Just another FYI... The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC you do either of these machines would work out great. Roxanne -Original Message- From: Josie Britton <[EMAIL PROTECTED]> To: Michael Bradley <[EMAIL PROTECTED]>; Histonet@lists.utsouthwestern.edu Sent: Tue, 18 Nov 2008 11:31 am Subject: RE: [Histonet] Dako Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] equipment maintenance
Hi Claire Try this company, Microscope & Mictorome Service Company. I use them and they are very good. Fred Siena - Tissue Techniques Pathology Labs Click to consolidate debt and lower month expenses. http://thirdpartyoffers.juno.com/TGL2141/fc/PnY6rw2PBHhUPXLfAg1a9hw70cTqssbeSKA8zMLpGeD0s5HrKgchz/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Dako
Just another FYI... The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC you do either of these machines would work out great. Roxanne -Original Message- From: Josie Britton <[EMAIL PROTECTED]> To: Michael Bradley <[EMAIL PROTECTED]>; Histonet@lists.utsouthwestern.edu Sent: Tue, 18 Nov 2008 11:31 am Subject: RE: [Histonet] Dako Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] equipment maintenance
Hey gang, and vendors: We have recently lost our cryostat repairman. Does anyone know of a company that will repair a Microm HM 505? All it needs is a fan motor, but we would like some possible preventative maintenance done as well. Plus someone to contact in case we need repairs in the future. Thanks! Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control slide storage
The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing problems
We had a problem with our recycler and xylene contamination so we now test each batch by adding water to a sample of recycled alcohol. If it turns cloudy we assumed that we had xylene contamination. We have lately tested commercial ethanols (no turbidity)before placing them on the processor and after we processing with them just once we have turbidity after adding a water to all the ethanols 70-100%. This is happening to all the alcohols on all of our processors. I am thinking that it is not a xylene contamination but something else maybe a leaching of plastic from the cassettes? Has this happened to anyone else? Can anyone give me any suggestions. Sharon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SPECIAL STAINER
We have used an automated special stainer for years and are now experiencing multiple problems. Therefore before we order from the same vendor I wondered what everyone else was using for silver stains such as Jones, Retic, and GMS. Just trying to evaluate if replacing instrument with newer model is going to be a positive step or should we look at someone else. thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 [EMAIL PROTECTED] This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] therapeutic antibody detection in mouse xenografts?
Hi All, I am wondering if anyone has experience showing therapeutic monoclonal antibody binding in mouse xenografts (human tumor cells injected subcutaneously in SCID mice and then dose treated with therapeutic antibody injected intraperitoneally). For instance, can trastuzamab (Herceptin) binding be seen in a breast tumor xenograft treated with Herceptin? I have tried using Dako's goat-anti mouse dextran HRP conjugated secondary antibody developed with DAB. I also tried using an Abcam goat-anti mouse antibody conjugated with biotin, then adding Vectors elite ABC, then developing with DAB. Both times I used FFPE tissue. In both techniques, results showed minimal to staining and showed no difference between mice treated with antibody and control mice treated. I would appreciate if anyone knew of published papers or have experience using IHC to show therapeutic antibody bound to tumor cell in mouse xenografts. Thanks -Sam Research Technician Dana Farber Cancer Institute Boston MA. The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Buffer.
PIPES-buffered glutaraldehyde has been used for subsequent electron micr= oscopy since the early 1970s but it has been shown to cause artifacts- = intracellular vesicles and myelin figures - when used for perfu= sion fixation of the brain. See Schultz RL= & Wagner DO (1986) Membrane alterations in cerebral cortex when= using PIPES buffer. J. Neurocytol. 1= 5: 461-469. The authors attributed these art= ifacts, which potentially could be mistaken for pathological changes,= to the low toxicity of PIPES. Apparently cacodylate or phosphate buff er prevented the metabolic changes that caused the artifacts during the = early phase of fixation, but PIPES allowed the changes to occur. <= BR> The reason for using cacodylate buffer rather tha= n phosphate is that it doesn't precipitate with calcium ions. A Ca s= alt in the fixative improves the immobilization of phospholipids in cell membranes. John KiernanAnatomy, UW= OLondon, Canada= = =- Original Messa= ge -From: Robert Richmond <[EMAIL PROTECTED]&=gt;Date: Sunday, November 16, 2008 14:13Subjec= t: [Histonet] Re: Buffer.To:[EMAIL PROTECTED] uthwestern.edu> About the potential toxicity of= arsenic-containingcacodylatebuffers:> >= A Histonetter who prefers to remain anonymous notes >>The= arsenic> discussion comes up on the Microscopy Listserve= r every once in while,> usually because someone is eith= er pregnant & concerned about exposure> or has sa= fety officers who freak out over the stuff. It was used in&g= t; the early days because phosphate buffers can interact with some <= BR>> of the> fixative combinations and leave pre= cipitates. Also, I'd suspect,> cacodylate is less= prone to beasties growing in stock solutions over> time.= The "modern" zwitterionic buffers are used a lot these &g t; days -> PIPES & that crowd - if you want to = avoid cacodylate & > phosphate.<<= > Those zwitterionic buffers are often referred to as Goo= d's > buffers or> Good buffers, since = they were introduced by Norman Good in the 1960's.> S= ee> http://en.wikipedia.org/wiki/Good's_buffers > > I don't know why they aren't commo= nly used as buffers for > histologic fixatives.=> > Bob Richmond>SamuraiPathologi= st> Knoxville TN> > ___ ___ 5F =2 6g= t; Histonet mailing list> [EMAIL PROTECTED] tern.edu> http://lists.utsouthwestern.edu/mailman= /listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Uneven H&E Staining
Sounds to me that your having a de-paraffining problem. Check your xylene or clearing agent, maybe there is water in them or they are not as clean as they should be. Are you on a daily rotation/change of staining/de-paraffin reagents? Maybe, your oven isn't hot enough to melt some paraffin off the slides before de-par! Josie Britton HT -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Gudrun Lang Sent: Wednesday, November 19, 2008 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: Uneven H&E Staining Are the coplin jars all equally filled? If the wash-jars are partly filled, the lower slides would be more differentiated than the upper. Gudrun -Ursprüngliche Nachricht- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Karla Arrington Gesendet: Mittwoch, 19. November 2008 02:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Uneven H&E Staining Recently we have had an issue with uneven H&E staining. Lately slides that have three sections on one slide, mostly GI biopsies, and the last section (bottom of slide) are paler than the upper two section. All cut by the same person and the stains were great. What could be the cause of this? Also with the quality of formalin. Can formalin be purchased as "bad" when it takes 6 days to fix placenta or breasts? What is the cause? Karla Arrington, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ThermoWave users
All ThermoWave Microwave Processing users Would any of you be filling to share your needle cor biopsy SOP (i.e. prostate) and the GI SOP? Thanks Roxanne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Telly's fixative. Also Bodian.
R. Tellyesniczky published more than one fixative, 1898-190= 5. His best-known one translates into AFA or FAA: mostly ethyl alcoh= ol, with about 4% formaldehyde and about 5% acetic acid. It was = reinvented in 1937 by David Bodian (Anat. Rec. 69: 153-162) as the best fixative for subsequent = staining of axons with his 1936 protargol method (Anat. Rec<= /EM>. 65: 89-97). AFA mixtures are gene= rally better than aqueous formaldehyde fixatives if you need to stain no= rmal or regenerating axonsinparaffinsections with any silver method. Bob Richmond may remember Bodian from Johns H= opkins. Bodian made important contributions to knowledge of functional= human neuroanatomy from clinico-pathological correlations in people wit= h poliomyelitis. He correlated the positions of virus-killed = motor neurons in the spinal cord with the muscles that were paralysed b= efore death. This was a big job! The adult human spinal cord is 45 cm = long, and neuronal columns can be appreciated only in transv= erse sections. John KiernanNeuroanat= omist at UWOLondon,Canada http://instruct .uwo.ca/anatomy/530/= = =- Original Messag= e -From: pam johnson <[EMAIL PROTECTED]><= BR>Date:Tuesday, November 18, 2008 12:14Subject: [= Histonet] Telly's fixativeTo: [EMAIL PROTECTED] .edu> > Does anyone have a recip= e for Telly's fixative?> > Thanks,<= BR>>Pam> _ ___ 5F= __> Histonet mailing list> Histonet@lists.utsouthwestern.edu> htt p://lists.utsouthwestern.edu/mailman/listinfo/histonet=3 CB= R> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Antibody protocol
We are using the ultraView DAB from Ventana and the same HSA from Cell Marque. Here is the protocol we are using. Depar as usual; CC1 Standard; HSA incubation at 37C for 32 min; Counterstain Heme 4 min; Post Counterstain Bluing for 4 min. we have found it to be a reliable stain and very clean and crisp. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jesus Ellin Sent: Tuesday, November 18, 2008 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody protocol Needing some help with the staining protocol for HSA (Hepatocyte Specific Antigen) clone OCH1E5, made by Cell marquee to be ran on the Ventana LT/Benchmark platform.. Would anyone be willing to share protocol information with us, as well as staining methodology. Thank you for the help. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 (Fax: (928) 336-7319 *Email: [EMAIL PROTECTED] This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Antibody protocol
We are using the Benchmark XT. Sorry I omitted that. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jesus Ellin Sent: Tuesday, November 18, 2008 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody protocol Needing some help with the staining protocol for HSA (Hepatocyte Specific Antigen) clone OCH1E5, made by Cell marquee to be ran on the Ventana LT/Benchmark platform.. Would anyone be willing to share protocol information with us, as well as staining methodology. Thank you for the help. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 (Fax: (928) 336-7319 *Email: [EMAIL PROTECTED] This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] C4d question (not CD4)
This biocare-antibody is CD4 and not complement4d. Gudrun -Ursprüngliche Nachricht- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Jodie Robertson Gesendet: Mittwoch, 19. November 2008 15:34 An: Richard Cartun; Histonet Betreff: RE: [Histonet] C4d question (not CD4) Biocare has an IVD predilute that is very good. (PM153AA) We use it on our Benchmark XT. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Chico, CA -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Richard Cartun Sent: Tuesday, November 18, 2008 3:16 PM To: Histonet Subject: [Histonet] C4d question (not CD4) We have been using an "RUO"-labeled anti-C4d antibody on certain renal transplant cases for the immunohistochemical detection of humoral rejection for the past several years. We have now been asked by our Transplant team to do this test routinely on all transplant kidney biopsies (which is another issue). Is anyone using an "IVD"-labeled antibody for C4d detection by IHC? Although we get excellent results with this antibody on formalin-fixed, paraffin-embedded tissue, I would prefer to be using an "IVD"-labeled antibody (if one exists). Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Re: Telly's fixative
You have to stress the first and the third syllable. Like "sun-shine-rag-gy" "Tell-yes-nicz-ky" Gudrun -Ursprüngliche Nachricht- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Robert Richmond Gesendet: Mittwoch, 19. November 2008 02:53 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: Telly's fixative I thought Gudrun Lang would know how to pronounce Tellyesniczky if anybody did! But one more question - which syllable is accented? Gudrun wrote: It's a Hungarian name. In Austria we have similar names. And you pronounce it: Tell - like "tell" yes - like "yes" nicz - like "bits" with "n" ky - like "key" Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] C4d question (not CD4)
Jodie, That's for CD4 not C4d Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jodie Robertson Sent: Wednesday, November 19, 2008 9:34 AM To: Richard Cartun; Histonet Subject: RE: [Histonet] C4d question (not CD4) Biocare has an IVD predilute that is very good. (PM153AA) We use it on our Benchmark XT. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Chico, CA -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Richard Cartun Sent: Tuesday, November 18, 2008 3:16 PM To: Histonet Subject: [Histonet] C4d question (not CD4) We have been using an "RUO"-labeled anti-C4d antibody on certain renal transplant cases for the immunohistochemical detection of humoral rejection for the past several years. We have now been asked by our Transplant team to do this test routinely on all transplant kidney biopsies (which is another issue). Is anyone using an "IVD"-labeled antibody for C4d detection by IHC? Although we get excellent results with this antibody on formalin-fixed, paraffin-embedded tissue, I would prefer to be using an "IVD"-labeled antibody (if one exists). Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] RE: Uneven H&E Staining
Are the coplin jars all equally filled? If the wash-jars are partly filled, the lower slides would be more differentiated than the upper. Gudrun -Ursprüngliche Nachricht- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Karla Arrington Gesendet: Mittwoch, 19. November 2008 02:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Uneven H&E Staining Recently we have had an issue with uneven H&E staining. Lately slides that have three sections on one slide, mostly GI biopsies, and the last section (bottom of slide) are paler than the upper two section. All cut by the same person and the stains were great. What could be the cause of this? Also with the quality of formalin. Can formalin be purchased as "bad" when it takes 6 days to fix placenta or breasts? What is the cause? Karla Arrington, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] C4d question (not CD4)
Biocare has an IVD predilute that is very good. (PM153AA) We use it on our Benchmark XT. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Chico, CA -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Richard Cartun Sent: Tuesday, November 18, 2008 3:16 PM To: Histonet Subject: [Histonet] C4d question (not CD4) We have been using an "RUO"-labeled anti-C4d antibody on certain renal transplant cases for the immunohistochemical detection of humoral rejection for the past several years. We have now been asked by our Transplant team to do this test routinely on all transplant kidney biopsies (which is another issue). Is anyone using an "IVD"-labeled antibody for C4d detection by IHC? Although we get excellent results with this antibody on formalin-fixed, paraffin-embedded tissue, I would prefer to be using an "IVD"-labeled antibody (if one exists). Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used Equipment
I am searching for a used tissue processor in good condition. Please contact me by e-mail: [EMAIL PROTECTED] Cindy DuBois Integrated Pathology Stockton, Ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question
And... How many of us have developed sensitivity to d-limonene? I for one have a sensitivity to it and more and more Mohs labs are forgoing fume hoods because it is considered 'safe'. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing & Mohs Suite Mohs Reporting Software 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com & www.mohssuite.com Email: [EMAIL PROTECTED] DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. Thank You. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Lee & Peggy Wenk Sent: Tuesday, November 18, 2008 5:29 PM To: 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; [EMAIL PROTECTED]; [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: RE: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question Actually, d-limonene is used to impart a lemon/orange flavor in lots of candy, drinks (soda), gum, ice cream, gelatin, cakes, etc. So you've been eating it all along! And putting it on your body in orange/lemon scented soap, detergent, creams, lotions, etc. And cleaning with it (think of all the cleaning solutions you have at home that smell like oranges) and car degreasers. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospitals Royal Oak, MI 48073 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, November 18, 2008 1:21 PM To: [EMAIL PROTECTED]; [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: RE: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question LOL! That is too funny! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Cheryl Sent: Tuesday, November 18, 2008 1:17 PM To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question Read down to Characteristics-- I want to know who they got to taste this stuff??!!! Cheryl _ HISTOCLEAR (C10H16) A naturally occurring hydrocarbon found in plants. SYNONYMS 1-methyl-4(1-methylethenyl) cyclohexane p-mentha-1,8-diene, d-limonene, Safsolv, Histolene, dipentene. GENERAL PRECAUTIONS Avoid skin contact, can cause dermatitis. Avoid inhalation. Keep away from heat or naked flames. Wash hands thoroughly after handling. CHARACTERISTICS Clear liquid of low viscosity with a fresh, light and sweet citrusy odour. The taste is sweet, refreshing, citrus-like and mild. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.9.4/1794 - Release Date: 11/17/2008 8:48 AM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laser Capture Microdissection Webinar
Announcing MDS Analytical Technologies next in the series of Laser Capture Microdissection and Microgenomics Webinars. Our next LCM webinar will be held on Monday, November 24, 2008 at 10AM PST or 1PM EST. The speaker will be Charmain Pietersen, PhD., Laboratory for Structural and Molecular Neuroscience, McLean Hospital. Her presentation is titled: "Gene expression analysis in homogenous single cell populations in the superior temporal gyrus in postmortem brains from subjects with schizophrenia". This webinar is free to all registrants. For further information and/or to register for this webinar, please connect to the following website: http://www.moleculardevices.com/pages/lcm_webinar_11.2008.html Shirley Chu Application Scientist, Arcturus LCM Products Molecular Devices (now a part of MDS Analytical Technologies) 408-747-3765 | www.moleculardevices.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Isopropyl
Good Morning, we are interested in replacing xylene in our lab. Is anyone using graded isopropyl alcohols in there processors instead of xylene and if so how is it working for you? Thanks. Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
