[Histonet] Wilder's Reticulum stain
--- On Thu, 1/15/09, Kathy Lambeth histoc...@yahoo.com wrote: From: Kathy Lambeth histoc...@yahoo.com Subject: Wilder's Reticulum stain To: histo...@list.utsouthwestern.edu Date: Thursday, January 15, 2009, 6:25 AM I just relocated to a new lab and they use Wilder's Retic stain. They have been unable to get uranium nitrate. Is there a substitute; another supplier; or different technic they should use. Any suggestions are welcome. Kathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Type I and TYpe II Immunos for fiber typing
Hi Everyone :) Does anyone do or know of antibodies for immuno staining of muscle for fiber typing?? If you do catalog numbers and company that you get the antibodies from would be greatly appreciated and also your procedure would also be nice :) Neuropath Nancy @ RIH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question about Mercedes Medical star frost slides, comments?
Hi All, I was wondering if anyone out there has used Mercedes medical starfrost adhesive slides and how they hold up in IHC. MER 7255 - Slides, adhesive, 90, Starfrost, 10 gross - $235. I am thinking about changing to these slides from our VWR superfrost plus slides to save money but I would like to hear how they hold up in IHC Ag retrieval etc... I have samples from them and will try them on some IHC but I thought I'd get someone else's thoughts. If you have an opinion on the quality of the slides please let me know. Thanks, Jamie ___ Jamie Erickson Sr. Research Associate II M.S. HTL (ASCP) Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erick...@abbott.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PA job
Geisinger Medical Center in Danville, PA is searching for two Histotechnologists. We have a dayshift 0.6 tech position and a nightshift 8P-4A FT position available. Visit our website at www.geisinger.org to see more information about our Health System. Contact me for more information at 570-214-9634 or send me an email. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] prion contaminated tissue processing
Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] prion contaminated tissue processing
It will not. Check out the CDC and/or the WHO guidelines. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Hardin Sent: Thursday, January 15, 2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prion contaminated tissue processing Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Work flow, quality issues
What is the gain??? I am confused by their methods. I also face(rough cut) and section on the same microtome. I noticed over the years that cuts on the same microtome and block, one tech can get a thin section just by the way they turn the flywheel. A heavy handed cutter can make the section thicker on the same instrument and block. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Wednesday, January 14, 2009 2:53 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Work flow, quality issues I have worked in several HT labs and as expected most differ in individual technique. Most are very good as far as work flow from grossing, processing, embedding and sectioning. I work in a lab now where the grossing and processing of like specimens are in case order until there embedded. Embedded totally out of order, even within a case. One person will rough cut the blocks on 1 microtome, approx 20 microns. After all the embedding is done the trimmed blocks are put in order, placed on ice in which about half are to be cut on another microtome. Although the microtomes are adjusted close I have found that by the time I'm ready to section my blocks on the microtome not used for trimming I often have to go into the tissue 20-40microns, 5 microns at a time, to get a complete section. This often makes it tough to get a good, rehydrated 2nd section not to mention often the 1st if the event the tissue is larger and or firm to start with. Often I have to re-trim the blocks to match my microtome then rehydrate them again. This all takes time and makes it impossible to section all my cases in order, waiting on blocks to rehydrate, hold slides sometimes leading to mistakes. I have always, and in every lab I worked except this one, trimmed my own blocks for a specific microtome. At the end of trimming, I always fine cut 4-5 microns 2-5 times to deminish the artifact often caused by too aggressive initial trimming. Then I rehydrate and ice the tissue.. With this technique I use less knifes also. I temped in this lab about 1/1/2 years ago and was asked by the Pathologist and Lab Director to address these very issues and section quality. Now that I'm back nothing has changed. The pathologist still has section quality issues. What ever happen to the idea of Quality Improvement. The works getting done I suppose, maybe thats all that matters these days. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Question about Mercedes Medical star frost slides, comments?
We use their cheaper ones - CAS 9308 W - and they work just fine. j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jamie E Erickson Sent: Thursday, January 15, 2009 10:28 AM To: histonet histonet Subject: [Histonet] Question about Mercedes Medical star frost slides,comments? Hi All, I was wondering if anyone out there has used Mercedes medical starfrost adhesive slides and how they hold up in IHC. MER 7255 - Slides, adhesive, 90, Starfrost, 10 gross - $235. I am thinking about changing to these slides from our VWR superfrost plus slides to save money but I would like to hear how they hold up in IHC Ag retrieval etc... I have samples from them and will try them on some IHC but I thought I'd get someone else's thoughts. If you have an opinion on the quality of the slides please let me know. Thanks, Jamie ___ Jamie Erickson Sr. Research Associate II M.S. HTL (ASCP) Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erick...@abbott.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] prion contaminated tissue processing
Joe, I do IHC for prion diseases in animals. No, tissue processing will NOT render prion-infected tissue safe for sectioning under normal conditions. Incubating in formic acid won't even totally inactivate the abnormal protein. We have to use a dedicated microtome in a dedicated room, wear nitrile gloves and disposable lab coats and booties, and stain the slides on dedicated autostainers. As others have said, check out the CDC website for more information. Jan Shivers UMN Vet Diag Lab - Original Message - From: Joe Hardin har...@oncology.wisc.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, January 15, 2009 9:29 AM Subject: [Histonet] prion contaminated tissue processing Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Work flow, quality issues
I have my techs be the only person who handles a block from facing off to finished microtomy. They put their initials on the slide label, so if that block needs recuts in the following days, the same tech and microtome will be used. That way, the least amount of tissue is lost off the block face. Jan Shivers UMN Vet Diag Lab - Original Message - From: Steven Coakley sjchta...@yahoo.com To: Histonet@lists.utsouthwestern.edu Sent: Wednesday, January 14, 2009 2:53 PM Subject: [Histonet] Work flow, quality issues I have worked in several HT labs and as expected most differ in individual technique. Most are very good as far as work flow from grossing, processing, embedding and sectioning. I work in a lab now where the grossing and processing of like specimens are in case order until there embedded. Embedded totally out of order, even within a case. One person will rough cut the blocks on 1 microtome, approx 20 microns. After all the embedding is done the trimmed blocks are put in order, placed on ice in which about half are to be cut on another microtome. Although the microtomes are adjusted close I have found that by the time I'm ready to section my blocks on the microtome not used for trimming I often have to go into the tissue 20-40microns, 5 microns at a time, to get a complete section. This often makes it tough to get a good, rehydrated 2nd section not to mention often the 1st if the event the tissue is larger and or firm to start with. Often I have to re-trim the blocks to match my microtome then rehydrate them again. This all takes time and makes it impossible to section all my cases in order, waiting on blocks to rehydrate, hold slides sometimes leading to mistakes. I have always, and in every lab I worked except this one, trimmed my own blocks for a specific microtome. At the end of trimming, I always fine cut 4-5 microns 2-5 times to deminish the artifact often caused by too aggressive initial trimming. Then I rehydrate and ice the tissue.. With this technique I use less knifes also. I temped in this lab about 1/1/2 years ago and was asked by the Pathologist and Lab Director to address these very issues and section quality. Now that I'm back nothing has changed. The pathologist still has section quality issues. What ever happen to the idea of Quality Improvement. The works getting done I suppose, maybe thats all that matters these days. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Question about Mercedes Medical star frost slides, comments?
I made the switch to Mercedes a while back. I use their clipped corner slides (MER7200) because they work great on our slide printer. I also use their Plus Starfrost slides (MER7255) for our IHC. The sections stay on great, even through antigen retrieval. Probably the best plus slide I have used. Try their samples, I'm sure you will be pleased. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 --- On Thu, 1/15/09, Weems, Joyce jwe...@sjha.org wrote: From: Weems, Joyce jwe...@sjha.org Subject: RE: [Histonet] Question about Mercedes Medical star frost slides, comments? To: Jamie E Erickson jamie.erick...@abbott.com, histonet histonet histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:39 AM We use their cheaper ones - CAS 9308 W - and they work just fine. j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jamie E Erickson Sent: Thursday, January 15, 2009 10:28 AM To: histonet histonet Subject: [Histonet] Question about Mercedes Medical star frost slides,comments? Hi All, I was wondering if anyone out there has used Mercedes medical starfrost adhesive slides and how they hold up in IHC. MER 7255 - Slides, adhesive, 90, Starfrost, 10 gross - $235. I am thinking about changing to these slides from our VWR superfrost plus slides to save money but I would like to hear how they hold up in IHC Ag retrieval etc... I have samples from them and will try them on some IHC but I thought I'd get someone else's thoughts. If you have an opinion on the quality of the slides please let me know. Thanks, Jamie ___ Jamie Erickson Sr. Research Associate II M.S. HTL (ASCP) Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erick...@abbott.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Work flow, quality issues
Steven: Are you just complaining to vent some sort of frustration, or can you actually do something about it? If venting, my sympathies go to you. What you describe could become the nightmare of anybody with the slightest idea about histology good practices, not to mention somebody that tries to take the flow into a more or less Lean semblance. The muda is astonishing. If you can do something about, what you describe you are used to do is just what has to be done. Use your knowledge and credentials to try to solve the mess you describe. You know how to do it and good luck. I am sure that somebody in that lab has done it that way for years and will be difficult to made to change. René J. --- On Wed, 1/14/09, Steven Coakley sjchta...@yahoo.com wrote: From: Steven Coakley sjchta...@yahoo.com Subject: [Histonet] Work flow, quality issues To: Histonet@lists.utsouthwestern.edu Date: Wednesday, January 14, 2009, 3:53 PM I have worked in several HT labs and as expected most differ in individual technique. Most are very good as far as work flow from grossing, processing, embedding and sectioning. I work in a lab now where the grossing and processing of like specimens are in case order until there embedded. Embedded totally out of order, even within a case. One person will rough cut the blocks on 1 microtome, approx 20 microns. After all the embedding is done the trimmed blocks are put in order, placed on ice in which about half are to be cut on another microtome. Although the microtomes are adjusted close I have found that by the time I'm ready to section my blocks on the microtome not used for trimming I often have to go into the tissue 20-40microns, 5 microns at a time, to get a complete section. This often makes it tough to get a good, rehydrated 2nd section not to mention often the 1st if the event the tissue is larger and or firm to start with. Often I have to re-trim the blocks to match my microtome then rehydrate them again. This all takes time and makes it impossible to section all my cases in order, waiting on blocks to rehydrate, hold slides sometimes leading to mistakes. I have always, and in every lab I worked except this one, trimmed my own blocks for a specific microtome. At the end of trimming, I always fine cut 4-5 microns 2-5 times to deminish the artifact often caused by too aggressive initial trimming. Then I rehydrate and ice the tissue.. With this technique I use less knifes also. I temped in this lab about 1/1/2 years ago and was asked by the Pathologist and Lab Director to address these very issues and section quality. Now that I'm back nothing has changed. The pathologist still has section quality issues. What ever happen to the idea of Quality Improvement. The works getting done I suppose, maybe thats all that matters these days. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Wilder's Reticulum stain
Try phosphotungstic acid at the same concentration. René J. --- On Thu, 1/15/09, Kathy Lambeth histoc...@yahoo.com wrote: From: Kathy Lambeth histoc...@yahoo.com Subject: [Histonet] Wilder's Reticulum stain To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 8:04 AM --- On Thu, 1/15/09, Kathy Lambeth histoc...@yahoo.com wrote: From: Kathy Lambeth histoc...@yahoo.com Subject: Wilder's Reticulum stain To: histo...@list.utsouthwestern.edu Date: Thursday, January 15, 2009, 6:25 AM I just relocated to a new lab and they use Wilder's Retic stain. They have been unable to get uranium nitrate. Is there a substitute; another supplier; or different technic they should use. Any suggestions are welcome. Kathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] prion contaminated tissue processing
NO, it will not. René J. --- On Thu, 1/15/09, Joe Hardin har...@oncology.wisc.edu wrote: From: Joe Hardin har...@oncology.wisc.edu Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Dako IHC Instrument and T.P.I.D.
I'm curious if anyone out there has any feedback or comments on the new Dako IHC Instrument, Autostainer Link 48 and/or their TPID (True Positive ID). We are in desparate need of replacing our current DAKO instruments, and are probably going to stick with DAKO again after looking at several other instruments. I just thought I check to see if anyone using it has anything to say. Beth ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] prion contaminated tissue processing
Viable prions have been found in 20 year old paraffin blocks.Only extended exposure to bleach will kill 'em - last I heard. Not formalin, not processing - nothin. Jackie O' Rene J Buesa rjbu...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 01/15/2009 11:32 AM Please respond to rjbu...@yahoo.com To histonet@lists.utsouthwestern.edu, Joe Hardin har...@oncology.wisc.edu cc Subject Re: [Histonet] prion contaminated tissue processing NO, it will not. René J. --- On Thu, 1/15/09, Joe Hardin har...@oncology.wisc.edu wrote: From: Joe Hardin har...@oncology.wisc.edu Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] prion contaminated tissue processing
Please read the protocols at the CDC and/or the WHO website. Bleach in not effective. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, January 15, 2009 12:40 PM To: rjbu...@yahoo.com Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] prion contaminated tissue processing Viable prions have been found in 20 year old paraffin blocks.Only extended exposure to bleach will kill 'em - last I heard. Not formalin, not processing - nothin. Jackie O' Rene J Buesa rjbu...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 01/15/2009 11:32 AM Please respond to rjbu...@yahoo.com To histonet@lists.utsouthwestern.edu, Joe Hardin har...@oncology.wisc.edu cc Subject Re: [Histonet] prion contaminated tissue processing NO, it will not. René J. --- On Thu, 1/15/09, Joe Hardin har...@oncology.wisc.edu wrote: From: Joe Hardin har...@oncology.wisc.edu Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paraffin
Hello Histonetters: We are currently using Surgipath's Formula R paraffin. They are now charging us a handling fee for each container. I have tried looking at different formulas at different companies trying to find a comparable formula. It seems that no one will list the polymer combinations or ingredients. I was wondering if anyone else had encountered this problem? Does anyone know of a paraffin type that is similar to or the same as the Formula R? I would appreciate any feedback that folks are willing to share. Thanks, Debbie Debbie Nannenga, HTL(ASCP),QIHC InCyte Pathology Spokane Valley, Washington ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] prion contaminated tissue processing
An article entitled Safe Handling of Transmissible Spongiform Encephalopathies Specimens in the Histopathology Laboratory written by Konnie Zeitner was published in the JOH June 2007.. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfie...@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, January 15, 2009 11:40 AM To: rjbu...@yahoo.com Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] prion contaminated tissue processing Viable prions have been found in 20 year old paraffin blocks.Only extended exposure to bleach will kill 'em - last I heard. Not formalin, not processing - nothin. Jackie O' Rene J Buesa rjbu...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 01/15/2009 11:32 AM Please respond to rjbu...@yahoo.com To histonet@lists.utsouthwestern.edu, Joe Hardin har...@oncology.wisc.edu cc Subject Re: [Histonet] prion contaminated tissue processing NO, it will not. René J. --- On Thu, 1/15/09, Joe Hardin har...@oncology.wisc.edu wrote: From: Joe Hardin har...@oncology.wisc.edu Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Prion Contamination
Not only will processing NOT render it safe, but your cut, stained, and mounted slides will still be infectious UNLESS the procedure to inactivate using formic acid is followed before fixation and processing. If the tissue is fixed (formalin or other common fixatives) then you would be actually fixing the prion's ability to NOT be inactivated. Prions are nasty little beasties, and best left to dedicated labs that do nothing else. Please refer to the link below for the WHO pdf document which will give you procedures for handling prion infected patient's and tissues. http://www.who.int/csr/resources/publications/bse/whocdscsraph2003.pdf Section 8.2.2 directly addresses the Histopathology techniques to be used when handling these tissues. So much about these self replicating proteins is unknown, and more forms are added to the list all the time. Currently, the CDC recognizes 5 human forms, including, Creutzfeldt-Jakob Disease (CJD) Variant Creutzfeldt-Jakob Disease (vCJD) Gerstmann-Straussler-Scheinker Syndrome Fatal Familial Insomnia Kuru There are also 6 Animal Prion Diseases: Bovine Spongiform Encephalopathy (BSE) Chronic Wasting Disease (CWD) Scrapie Transmissible mink encephalopathy Feline spongiform encephalopathy Ungulate spongiform encephalopathy See the CDC link below for more information http://www.cdc.gov/ncidod/dvrd/prions/ and above all, Please, Please, Please be very careful. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --- On Thu, 1/15/09, Joe Hardin har...@oncology.wisc.edu wrote: From: Joe Hardin har...@oncology.wisc.edu Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] prion contaminated tissue processing
exposure to bleach will kill 'em - last I heard. think about it... if that were the case, things like BSE and CJD wouldn't be able to taint meat-processing equipment, which is generally about as awash in bleach as anything could be, at least once per shift. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Autoflourescence
Hi- I am having a horrible time with autofluorescence in my human brain FFPE tissue. I have been using Sudan Black which helps a little, but the background is still pretty bad. The tissue is usually cut at 10-15um. Does anyone have any other suggestions? Has anyone tried photobleaching the tissue prior to staining with any success? I attempted this once with no luck... Any help would be very much appreciated! Thanks in advance. Nicole ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Autoflourescence
10-15 um seems extremely thick for paraffin sections. Can you get thinner sections? Say no thicker than 6um? Unfortunately autofluorescence is a fact of life with FFPE sections, hence why people normally do not choose to do immunofluorescence on FFPE sections. Are you tethered to using immunofluorescence? There are alternatives. - Original Message - From: Patten, Nicole (NIH/NIAAA) [F] patte...@mail.nih.gov Date: Thursday, January 15, 2009 4:36 pm Subject: [Histonet] Autoflourescence Hi- I am having a horrible time with autofluorescence in my human brain FFPE tissue. I have been using Sudan Black which helps a little, but the background is still pretty bad. The tissue is usually cut at 10-15um. Does anyone have any other suggestions? Has anyone tried photobleachingthe tissue prior to staining with any success? I attempted this once with no luck... Any help would be very much appreciated! Thanks in advance. Nicole ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Autoflourescence
The Wright Cell Imaging Facility at University Health Network in Toronto has compiled information on reducing autofluorescence from the Histonet archives into a booklet. Very useful. http://www.uhnres.utoronto.ca/facilities/wcif/fdownload2.html Bob On Thu, Jan 15, 2009 at 1:36 PM, Patten, Nicole (NIH/NIAAA) [F] patte...@mail.nih.gov wrote: Hi- I am having a horrible time with autofluorescence in my human brain FFPE tissue. I have been using Sudan Black which helps a little, but the background is still pretty bad. The tissue is usually cut at 10-15um. Does anyone have any other suggestions? Has anyone tried photobleaching the tissue prior to staining with any success? I attempted this once with no luck... Any help would be very much appreciated! Thanks in advance. Nicole ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thick paraffin sections for immunostaining
We would like to do immunostaining on thick FFPE sections for neuronal markers such as tyrosine hydroxylase so that we may trace neurons contained in non-brain tissue. Section thickness will probably be in the order of 40-50 microns, though perhaps thicker too. I have searched histonet archives but have only found references to vibratome sections of brain tissue, wholemount embryos and free-floating sections that I assume were cut on a cryostat. Antibody penetration has been achieved using DMSO, ethanol extraction, acetone, triton-x 100 etc, but I was wondering if anybody had managed to use any of these methods successfully on thick paraffin sections of non-brain tissue - our tissue is predominantly connective / adipose in nature. It is possible that we could do vibratome sections if we had to, but the tissues have already been collected, processed and paraffin blocked and would rather not have to do further experiments and tissue collection. I did also note in the archives that in regular paraffin sections, antibody penetration and thus labelling with standard methods is only thought to be of the order of 2 microns. Many thanks, Jason Jason Palmer Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.pal...@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email _ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
