[Histonet] BrdU, CldU and IdU nucleotide labeling
Hi all, I would like to label slow and fast cycling progenitor cells using double nucleoside analog labeling. Can BrdU be distinguished from CldU and/or IdU? Maslov et al (J. Neurosci, 24:1726--1733, 2004) used anti-BrdU antibodies to show both. I assume, one stains one slide of BrdU only treated tissue with anti-BrdU to identify one set of progenitors, and then stains the slides of tissue treated with both (for example) BrdU and CldU and then substracts the first result from the last to get the difference? Or are there specific antibodies to BrdU, CldU and IdU that are available? Can anyone supply a copy of Aten et al (Meth. Cell Biol. 41:317--326, 1994)? Its the reference for the original protocol---unfortunately not available to me. :-( A protocol would be just as welcome. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@freeshell.org tel: +27-84-632-1925 (c) For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all. 1 Timothy 2:5-6 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Obtaining control tissue
Hello histonetters! I am looking at obtaining control tissue for special stain controls and IHC controls. I think there is a tissue bank but I do not know who to contact. Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x104 shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68 counts
I am attempting to accurately assess the number of CD 68 positive cells (hopefully macrophages) in biopsies, they have been GMA processed, ABC staining, with AEC visualisation; no problem with a nucleated cell with positive cell membrane, but they are in the minority, so the question how does one judge the cut-off point that makes a positive cell into a negative cell if you catch my drift??. I am quite experienced at counting cells but feel that obtaining reliable, consistent results from this material is quite challenging. All ideas/thoughts gratefully received Cheers Richard Edwards University of Leicester U.K. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we
Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain? Thanks in advance Sarah Reeves ** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmas...@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Background
Hello everyone! I was wondering if anyone has ever pre-incubated the antibody with normal Mouse IgG to get rid of the unspecific staining. The antibody I use crosreacts with mouse , and both human and mouse tissues stain within a xenograft. I've heard that one can get rid of the mouse staining by incubating with murine IgG. Has anyone ever done that and if so, you mind sharing some info? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decal billing issue
Good day all, I have a billing issue and need some input. How do you all bill decals... per container or by block or just by case? Thanks for your input, Tere Hodges St. Mary's Hospital Tucson,Az. _ Windows Live™ Hotmail®…more than just e-mail. http://windowslive.com/howitworks?ocid=TXT_TAGLM_WL_t2_hm_justgotbetter_howitworks_012009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] decal billing issue
Same here..by each specimen container... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 3 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, January 21, 2009 1:18 PM To: MARY T HODGES; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal billing issue Per specimen here... Joyce -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MARY T HODGES Sent: Wednesday, January 21, 2009 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal billing issue Good day all, I have a billing issue and need some input. How do you all bill decals... per container or by block or just by case? Thanks for your input, Tere Hodges St. Mary's Hospital Tucson,Az. _ Windows Live(tm) Hotmail(r)...more than just e-mail. http://windowslive.com/howitworks?ocid=TXT_TAGLM_WL_t2_hm_justgotbetter_ howitworks_012009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: tissue processors
hello, we are in the market for a new/replacement tissue processor. If you have experience and/or pertinent detailed info on any of the following will you please share with me ASAP? *1.*ThermoShandon Citadel 2000 Tissue Processor, *2.* Leica TP 1020 Automatic Tissue Processor, and last but not least *3.* VIP 1000 Floor Tissue Processor. Thank you kindly. Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: tissue processors
I worked with a VIP 1000 well over 20 years ago. This is a dinosaur! Issues that will come to haunt you: The retort lid will warp over time (if it isn't already warped). Minor overtensioning of the clamps cause this. Many years ago we were told that new lids were no longer available. The printed circuit boards failed on a regular basis. Last I heard, they were no longer made. Perhaps an aftermarket parts are now available for these issues. I remember these units were real work horses. But they had no where near the bells and whistles you get with more modern units. Good luck! Joe From: Atoska Gentry gent...@vetmed.auburn.edu To: Histonet histo...@pathology.swmed.edu Sent: Wednesday, January 21, 2009 5:30:54 PM Subject: [Histonet] Re: tissue processors hello, we are in the market for a new/replacement tissue processor. If you have experience and/or pertinent detailed info on any of the following will you please share with me ASAP? *1.*ThermoShandon Citadel 2000 Tissue Processor, *2.* Leica TP 1020 Automatic Tissue Processor, and last but not least *3.* VIP 1000 Floor Tissue Processor. Thank you kindly. Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Auto-IHC Staining Issues
Hello, I've been having a problem with the staining quality from an auto-IHC stainer and I was wondering if any of you experience the same thing. I notice sometimes that the staining in the tissue isn't complete, like there are areas where the fluid (one or more of the steps involved) didn't get in contact with the tissue, thus resulting in patchy, skipped staining. I'm thinking that this must be a dispensing problem. Is this a general thing with all auto stainers - ones like DAKO or Lab Vision's stainer or Biocare Medical's stainer? These stainers dispense fluid onto the slide. I hope this explains things and I really hope that I can get some feedback. It's getting very frustrating. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] job posting
Seventh Wave Laboratories, located 30 miles west of downtown St. Louis, MO is currently seeking a Histotechnologist whose primary functions will be microtomy and staining of histological specimens. Other duties may include tissue trimming, processing, and embedding. Interested candidates may send a resume to: Seventh Wave Laboratories - Human Resources, 743 Spirit 40 Park Drive, Suite 209, Chesterfield, MO 63005 or FAX 636.519.4886 or h...@7thwavelabs.com. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] double staining on the ventana XT
Greetings, We are looking into doing some immuno double staining. I searched the archives and found a few similar inquiries but no responses. Anyone with experiences to share I would love to hear what you have to say. Everything from what not to do to what works well for you. Many Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Auto-IHC Staining Issues
Hi Paula, You really have to tell us which instrument you are using to get a concise answer. Other details like how the slides are dewaxed for instance would also definately help. Regards, Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Paula Lucas plu...@biopath.org 01/21/09 8:04 PM Hello, I've been having a problem with the staining quality from an auto-IHC stainer and I was wondering if any of you experience the same thing. I notice sometimes that the staining in the tissue isn't complete, like there are areas where the fluid (one or more of the steps involved) didn't get in contact with the tissue, thus resulting in patchy, skipped staining. I'm thinking that this must be a dispensing problem. Is this a general thing with all auto stainers - ones like DAKO or Lab Vision's stainer or Biocare Medical's stainer? These stainers dispense fluid onto the slide. I hope this explains things and I really hope that I can get some feedback. It's getting very frustrating. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] double staining on the ventana XT
Hi Jennifer, Your Ventana Application Specialist should be more than willing to help you thru this process. I demo'd an XT last year and I recall double-staining was very straight forward. Unfortunately the details of which were not retained by me! Good luck. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson histoi...@comcast.net 01/21/09 8:41 PM Greetings, We are looking into doing some immuno double staining. I searched the archives and found a few similar inquiries but no responses. Anyone with experiences to share I would love to hear what you have to say. Everything from what not to do to what works well for you. Many Thanks, Jennifer - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
