[Histonet] thanks
thanks to all who replied to my query regarding slide filing pages - I have lots of browsing to do today - yay!!! have a great weekend! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] sodium 5.5-diehylbarbiturate
Hi, I need help with ATPase muscle fiber staining in lambs. I am investigating the skeletal muscle fibers in lambs breaded extensively in mount-hills regions in Macedonia. But, I have a problem with a one chemical - sodium 5.5-diehylbarbiturate. The problem is that I can't get this substance in my country. So please, does any one know substitute or a protocol without Sodium barbital? Any help you can give me would be greatly appreciated. Lazo ass. m-r Lazo Pendovski, M.Sc., DVM University of St. Cyril and Methodist Faculty of Veterinary medicine Department of Functional Morphology Lazar Pop- Trajkov 5-7 1000 Skopje, R. of Macedonia tel: +389 2 3240 710 fax: +389 2 3114 619 mob:+389 70 766 017 e-mail: lpendov...@fvm.ukim.edu.mk ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Brazilliant
For general information: Brazilliant is the product name for Anatech's red nuclear stain solution. It is available and will continue to be available for the foreseeable future. The dye brazilin used to prepare Brazilliant also will continue to be available from Anatech. Ada T. Feldman ANATECH LTD. 1020 Harts Lake Road Battle Creek, MI 49037 Phone: 800.262.8324 Fax: 269.964.8084 email: adafeld...@anatechltdusa.com website: www.anatechltdusa.com On Feb 26, 2009, at 11:42 PM, Robert Richmond wrote: Sarah Jones at Dako responds - and Sarah, please forgive me for quoting you onto Histonet, but this seems to me to be a public message: Brazilliant will soon not be available for some time. The government in the state of Pernambuco in Brazil has declared a 10 year moratorium on the felling of the Pernambuco trees from which this dye is made. This took place in September of 2007, if I remember correctly. Violin bows are also made from this tree. I was doing some work here at Dako trying to use it in place of Nuclear Fast Red. But when I learned of this moratorium (at the Biological Stain Commission Annual Meeting in June of 2008), I stopped working on it. I believe I sent you some of my photomicrographs taken at that time. I never heard back from you, so I thought you lost interest. I can send them to you again if you want me to. Just let me know. And Allen Smith responded: I have used brazalum, made by substituting brazilin for hematoxylin in the recipe for Mayer's hemalum. It stains nuclei a slightly deeper red than nuclear fast red. Colorfastness is excellent. I have 40-year old slides stained with brazalum that still look as they did on the day I made them. Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine ** I think this clarifies the problem. I would hope that something could be worked out with the Brazilian government for the very small amount of brazil wood histologists require, but the Brazilian government has been difficult about issues like this - see a number of citations in Science over the last few years. Sarah, I was much impressed by your photomicrographs of stains with brazalum nuclear counterstaining, and I apologize for having failed to reply earlier. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Brazilliant
Ada Feldman at Anatech, thanks for setting us all straight about the availability of brazilin and Brazilliant. I think it's very important that this dye get into histologic use, given the uncertain future of nuclear fast red. Apparently Anatech has found a source for brazilin other than the endangered brazilwood (Caesalpinia echinata). According to the Wikipedia article http://en.wikipedia.org/wiki/Brazilin several other species of Caesalpinia contain brazilin. Ada, would it be appropriate to ask what Anatech's source is? That Wikipedia article doesn't mention the histologic use of brazilin. much less contain a photomicrograph. Could somebody fix that? - I haven't yet learned how to edit Wikipedia. Bob Richmond Samurai Pathologist Knoxville TN *** On Fri, Feb 27, 2009 at 12:11 PM, Ada Feldman adafeld...@anatechltdusa.com wrote: For general information: Brazilliant is the product name for Anatech's red nuclear stain solution. It is available and will continue to be available for the foreseeable future. The dye brazilin used to prepare Brazilliant also will continue to be available from Anatech. Ada T. Feldman ANATECH LTD. 1020 Harts Lake Road Battle Creek, MI 49037 Phone: 800.262.8324 Fax: 269.964.8084 email: adafeld...@anatechltdusa.com website: www.anatechltdusa.com On Feb 26, 2009, at 11:42 PM, Robert Richmond wrote: Sarah Jones at Dako responds - and Sarah, please forgive me for quoting you onto Histonet, but this seems to me to be a public message: Brazilliant will soon not be available for some time. The government in the state of Pernambuco in Brazil has declared a 10 year moratorium on the felling of the Pernambuco trees from which this dye is made. This took place in September of 2007, if I remember correctly. Violin bows are also made from this tree. I was doing some work here at Dako trying to use it in place of Nuclear Fast Red. But when I learned of this moratorium (at the Biological Stain Commission Annual Meeting in June of 2008), I stopped working on it. I believe I sent you some of my photomicrographs taken at that time. I never heard back from you, so I thought you lost interest. I can send them to you again if you want me to. Just let me know. And Allen Smith responded: I have used brazalum, made by substituting brazilin for hematoxylin in the recipe for Mayer's hemalum. It stains nuclei a slightly deeper red than nuclear fast red. Colorfastness is excellent. I have 40-year old slides stained with brazalum that still look as they did on the day I made them. Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine ** I think this clarifies the problem. I would hope that something could be worked out with the Brazilian government for the very small amount of brazil wood histologists require, but the Brazilian government has been difficult about issues like this - see a number of citations in Science over the last few years. Sarah, I was much impressed by your photomicrographs of stains with brazalum nuclear counterstaining, and I apologize for having failed to reply earlier. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Quality assurance program
I would like to do something different (more) than what we are doing right now with qa/qc. We write everything down that we do so we can track it and then when there are mistakes I will talk to the person who makes them. What are people doing in their labs to stop these mistakes in the first place? I know there are tools out there like L.E.A.N, but I was hoping to go another route for now. I have added more check lists and made many process changes but sometimes I feel as though people need extra incentive to do well. Sad but true. Thanks for any help!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vacation reply
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[Histonet] Embedding bone marrow aspirates
Hello Colleagues, I'm looking for opinions/experience regarding the following idea: I am thinking about using Histogel (not pushing this product over another, just don't know what else to call it!!) to localize the aspirate to a more confined area, hopefully more consistently on a single plane, and perhaps improve the cutability of the more brittle, crumbly specimens. Currently we filter BM aspirates with a piece of lenspaper, scrape the tissue from the filter onto a smaller piece of lenspaper that is then folded and placed between 2 biopsy pads in a cassette. Then the embedder has to scrape the processed material into the embedding mold and hopefully get most of it in the same plane (which he does reasonably well). What I would like to do is scrape most of the tissue to the bottom of the lenspaper filter cone, add histogel and then place the resulting pellet in a cassette for subsequent processing. I look forward to reading your knowledgeable replies. Have a nice weekend everyone. Sincerely, Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sulfated Alcian Blue
I need some help, has any one used the sulfated alcian blue to stain for amyloid. We have a case that looks like it should be positive but it is not staining with the Congo Red. Any help would be great. Nathaniel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Ideal fixation of mouse prostate
Vanessa, Overnight fixation should not be a problem for these samples depending on what will be done to them at a later time. For samples needing studies for mRNA or some IHC markers, you want to avoid prolonged exposure to formalin fixation. You'll need to optimize your fixation based on those needs. If it is for HE or routine special stains only, overnight is fine. Regarding leaving in 25% alcohol, that also shouldn't be a problem but if it isn't necessary, why do it? We routinely fix our samples overnight, and then dehydrate to 70% alcohol where they may have to sit until we can get them on the processor if it is already running another program. Our tissue processor starts in 70% alcohol. We have not experienced any issues with having tissue samples too brittle from sitting in this alcoholic solution. For paraffin processing, you should only need 10-20 minutes per station on the VIP processor. You should avoid extended times in absolute alcohol and xylenes because your samples will get overly brittle. We have one xylene station on our processor, followed by two stations of Clear-Rite 3. We use the one station of xylene because it is more tolerant to water than the Clear-Rite 3. There is generally enough humidity around here that it may affect our processor solutions. The Clear-Rite 3 completes the clearing step and is a better agent (in my opinion) for mouse tissues than using 3 changes of xylene. Others on this list use xylene routinely with no trouble. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help, Histotechnologist with grossing experience
Hi members of Histonet, I am having a hard time finding Histotechnologists in the Boston, MA area who have experience in Grossing ... could someone help me or guide me in the right direction? It looks like I can only find PA's who have this specific skill. I am recruiting around a Histotech, Grosser position in a non-hospital clinical lab and my client has request someone who is HT or HTL, but not a PA. Any help would be greatly appreciated. Melissa Ribeiro Healthcare Division Brine Group Staffing Solutions 20 Mall Road, Suite 225 Burlington, MA 01803 mribe...@brinegroup.com Ph. (781) 272-3400 ext. 228 Fax (781) 494-3401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sulfated Alcian Blue
We use it all the time. What can I do to help? J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nathanial nauss Sent: Friday, February 27, 2009 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sulfated Alcian Blue I need some help, has any one used the sulfated alcian blue to stain for amyloid. We have a case that looks like it should be positive but it is not staining with the Congo Red. Any help would be great. Nathaniel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: Nuclear fast red
My sincere apologies...I uploaded the previous Histonet list because I forgot to enter the appropriate re: in subject of email. Contrite carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tube station
I always preferred King's Cross myself, but I was only visiting... Claire PS, Don't confuse us poor Americans. We're used to the 'L', etc. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Edwards, R.E. Sent: Thu 2/26/2009 3:32 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tube station There is only one tube station, Mornington Crescent!!. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tube station
Oh... Now I get it!!! Duh... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Friday, February 27, 2009 5:41 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tube station I always preferred King's Cross myself, but I was only visiting... Claire PS, Don't confuse us poor Americans. We're used to the 'L', etc. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Edwards, R.E. Sent: Thu 2/26/2009 3:32 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tube station There is only one tube station, Mornington Crescent!!. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
