Re: [Histonet] BrdU immunostain on rat brain section

[TF]

hi, we worked a lot on this.
I prefer to use 40 um frozen sections.
Abcam anti-BrdU (rat sourced) is the best ever I know. It does not have high 
background with rat IgG. Some other mouse-/sheep- sourced BrdU are available.


2009-03-17 



TF 



发件人： Amy Lee 
发送时间： 2009-03-17  05:02:28 
收件人： histonet 
抄送： 
主题： [Histonet] BrdU immunostain on rat brain section 
 
Hello,
I was asked to do BrdU immunostain on rat brain tissue. Does anyone have 
experience of it? 
1.Do you prefer frozen or paraffin? 
2.Which antibody you recommend?
3.營f牋35um frozen is requied, what thickness you will use if you want go for 
paraffin?
?
?
Thanks,
Amy
  
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[Histonet] Fix Sediment Method

[Knutson, Deanne]

Fellow Histonetters - I am looking for feedback on the alternative methods
to prepare Non GYN cellblocks.  We currently use the Fix Sediment Method
where you spin the fluid to achieve a button, pour off the supernatant, and
pour formalin onto the button to harden it.  What do others do?  Does anyone
use the Bacterial Agar Method?  Thank you in advance for any information and
advice you can send me.

 

Deanne Knutson 

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[Histonet] BrdU immunostain on rat brain section

[Amy Lee]

Hello,
I was asked to do BrdU immunostain on rat brain tissue. Does anyone have 
experience of it? 
1.Do you prefer frozen or paraffin? 
2.Which antibody you recommend?
3. If  35um frozen is requied, what thickness you will use if you want go for 
paraffin?
 
 
Thanks,
Amy



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RE: [Histonet] grossing

[dcojita]

According to CLIA, the employee who grosses must also  possess a state
license when working in a state that requires licensure, such as Florida.
Keep in mind that CAP rules do not apply for those laboratories that are not
CAP accredited. 

Exert from CLIA - 

"In order to qualify as high complexity testing personnel under 
Sec. 493.1489(b)(3), the individual must have met or could have met the 
following qualifications for technologist as they were in effect on or 
before February 28, 1992. Each technologist must--
(a) Possess a current license as a laboratory technologist issued by 
the State, if such licensing exists;"


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
godsgal...@aol.com
Sent: Monday, March 16, 2009 3:33 PM
To: histonetal...@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] grossing

Actually, as long as they meet the standards for High Complexity testing
they can gross...according to CAP you have to have no knowledge of
anatomy to process.

Roxanne


-Original Message-
From: Histonet Alias 
To: godsgal...@aol.com
Cc: histonet@lists.utsouthwestern.edu
Sent: Mon, 16 Mar 2009 1:40 pm
Subject: Re: [Histonet] grossing


This is another famous gray area that CAP has left us. Are your techs
grossing or processing? We split up specimens between processing and
grossing. A PA will to the grossing and a technician will do the processing.
We have a PA or Path on call for any questions in the evening. If the tech
is unsure then the PA or Path comes in or leaves it until the next day.?

ANP.11600
1. Processing is defined as a tissue examination limited to description,
inking and cutting of the specimen (if applicable), and submission of
the entire specimen to histology.  Tissue processing can be performed
according to standardized protocols.  Processing is generally limited to
small specimens (skin, small biopsies, curettings, etc.) and does not
require knowledge of anatomy.
2.  Grossing (or gross examination) is defined as a tissue examination
requiring a greater exercise of judgment and a knowledge of anatomy.
Dissection of the specimen and selection of tissue samples for
submission to histology are generally required.  The specimen
description is not necessarily standardized.


On Mon, Mar 16, 2009 at 12:11 PM,  wrote:

Processing, according to CAP? :

"tissue examination limited to description, inking and cutting of the
specimen, and submission og the entire specimen to histology.? Tissue
processing can be performed accodring to standardized protocols.? Processing
is generally limited to small specimens and does not require knowledge of
anatomy."

This is right from the CAP checklist (ANP.11600)

Roxanne






-Original Message-
From: Histonet Alias 
To: godsgal...@aol.com
Cc: histonet@lists.utsouthwestern.edu
Sent: Mon, 16 Mar 2009 11:13 am
Subject: Re: [Histonet] grossing



Define presence? If they are able to have access a technical supervisor or a
Pathologist if they have a question then this may just cover it. We have a
Pathologist "on call" that can be in within minutes if there are any
questions.?


On Mon, Mar 16, 2009 at 10:55 AM,  wrote:

I hate to beat a dead horse.but how do you handle non-certified grossing
staff.? They all meet the CLIA guidelines, but are they allowed to gross in
the evening without the presence of a technical supervisor?

And how do you handle salaries for those that process and those that
gross..according to the CAP guidelines?


Thanks,
Roxanne

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Al Ias HT(ASCP)
Histology Manager
Pathology Laboratory
United States



A Good Credit Score is 700 or Above. See yours in just 2 easy steps! 





-- 
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Histology Manager
Pathology Laboratory
United States


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Re: [Histonet] grossing

[godsgalnow]

Actually, as long as they meet the standards for High Complexity testing they 
can gross...according to CAP you have to have no knowledge of anatomy to 
process.

Roxanne


-Original Message-
From: Histonet Alias 
To: godsgal...@aol.com
Cc: histonet@lists.utsouthwestern.edu
Sent: Mon, 16 Mar 2009 1:40 pm
Subject: Re: [Histonet] grossing


This is another famous gray area that CAP has left us. Are your techs grossing 
or processing? We split up specimens between processing and grossing. A PA will 
to the grossing and a technician will do the processing. We have a PA or Path 
on call for any questions in the evening. If the tech is unsure then the PA or 
Path comes in or leaves it until the next day.?

ANP.11600
1. Processing is defined as a tissue examination limited to description,
inking and cutting of the specimen (if applicable), and submission of
the entire specimen to histology.  Tissue processing can be performed
according to standardized protocols.  Processing is generally limited to
small specimens (skin, small biopsies, curettings, etc.) and does not
require knowledge of anatomy.
2.  Grossing (or gross examination) is defined as a tissue examination
requiring a greater exercise of judgment and a knowledge of anatomy.
Dissection of the specimen and selection of tissue samples for
submission to histology are generally required.  The specimen
description is not necessarily standardized.


On Mon, Mar 16, 2009 at 12:11 PM,  wrote:

Processing, according to CAP? :

"tissue examination limited to description, inking and cutting of the specimen, 
and submission og the entire specimen to histology.? Tissue processing can be 
performed accodring to standardized protocols.? Processing is generally limited 
to small specimens and does not require knowledge of anatomy."

This is right from the CAP checklist (ANP.11600)

Roxanne






-Original Message-
From: Histonet Alias 
To: godsgal...@aol.com
Cc: histonet@lists.utsouthwestern.edu
Sent: Mon, 16 Mar 2009 11:13 am
Subject: Re: [Histonet] grossing



Define presence? If they are able to have access a technical supervisor or a 
Pathologist if they have a question then this may just cover it. We have a 
Pathologist "on call" that can be in within minutes if there are any questions.?


On Mon, Mar 16, 2009 at 10:55 AM,  wrote:

I hate to beat a dead horse.but how do you handle non-certified grossing 
staff.? They all meet the CLIA guidelines, but are they allowed to gross in the 
evening without the presence of a technical supervisor?

And how do you handle salaries for those that process and those that 
gross..according to the CAP guidelines?


Thanks,
Roxanne

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-- 
Al Ias HT(ASCP)
Histology Manager
Pathology Laboratory
United States



A Good Credit Score is 700 or Above. See yours in just 2 easy steps! 





-- 
Al Ias HT(ASCP)
Histology Manager
Pathology Laboratory
United States


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RE: [Histonet] decolorizing MMA

[Jack Ratliff]

Are you talking about thin microtomed sections or thicker "ground" sections 
Thin MMA embedded sections can be deplastified similar to the removal of 
paraffin embedded specimens, but thicker "ground" MMA sections cannot be 
deplastified.

 

However, maybe you are making reference to re-staining a slide like in a 
paraffin embedded H&E where you can reverse the staining order and stain 
again I have not tried it on thin sections and suspect that it would depend 
upon the stain used. Also, you might have a problem with the section becoming 
loose or falling off the slide.

 

As for the thicker "ground" sections, you should be able to re-polish the 
slides (depending upon true section thickness) and then stain again. Keep in 
mind that when staining these thicker MMA sections, most (if not all) typical 
stains only surface stain the section in the first place due to the size of the 
staining molecules and their ability to penetrate the resin w/ and/or w/o acid 
etching techniques.

 

Jack Ratliff

 


 
> Date: Mon, 16 Mar 2009 08:14:23 -0500
> From: bmolin...@heart.thi.tmc.edu
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] decolorizing MMA
> 
> Good morning,
> 
> Is it possible to decolorize MMA slides? 
> 
> Thanks,
> 
> Betsy
> 
> 
> 
> Betsy Molinari HT(ASCP)
> 
> Texas Heart Institute
> 
> Cardiovascular Pathology
> 
> 6770 Bertner Ave
> 
> MC1-283
> 
> Houston,TX 77030-2607
> 
> 832-355-6524
> 
> 832-355-6812
> 
> 
> 
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[Histonet] Histotechnologist Position in Boston-Direct Hire

[Alyssa Peterson]

Hello Histonetters!



I hope everyone had a great weekend. I wanted to follow up in reference to a
full time, direct hire position for a histotechnologist in Boston. My
company offers a referral bonus up to $1000 paid to you if your referral is
hired. Forward this to anyone you feel fit.



 If you are interested please respond with a current copy of your resume
attached as a Microsoft word document, and a contact number with the best
time to reach you. You can call me anytime, at 770.621.2639 ext. 4 or send
me an email at aly...@alliedsearchpartners.com for further information.

 **

*Please review the job description:*



Must have ASCP certification, with at least 2 year experience. Full time
regular employment, Day Shift, 6am-2:30pm.



*Benefits: *



My client offers an attractive benefits package for full time employment,
including medical/dental insurance, PTO, 401K, and much more!!

Salary is the highly competitive based on experience.



-- 
Alyssa Peterson
Allied Search Partners
O: 770.621.2639 ext. 4
F: 770.621.2640
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[Histonet] Looking for Patsy Ruegg's e-mail address

[Perry, Margaret]

Patsy I have a couple of questions for you but have the wrong address.
Margaret Perry

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Re: [Histonet] grossing

[Histonet Alias]

This is another famous gray area that CAP has left us. Are your techs
grossing or processing? We split up specimens between processing and
grossing. A PA will to the grossing and a technician will do the processing.
We have a PA or Path on call for any questions in the evening. If the tech
is unsure then the PA or Path comes in or leaves it until the next day.

ANP.11600
1. Processing is defined as a tissue examination limited to description,
inking and cutting of the specimen (if applicable), and submission of
the entire specimen to histology.  Tissue processing can be performed
according to standardized protocols.  Processing is generally limited to
small specimens (skin, small biopsies, curettings, etc.) and does not
require knowledge of anatomy.
2.  Grossing (or gross examination) is defined as a tissue examination
requiring a greater exercise of judgment and a knowledge of anatomy.
Dissection of the specimen and selection of tissue samples for
submission to histology are generally required.  The specimen
description is not necessarily standardized.


On Mon, Mar 16, 2009 at 12:11 PM,  wrote:

> Processing, according to CAP  :
>
> "tissue examination limited to description, inking and cutting of the
> specimen, and submission og the entire specimen to histology.  Tissue
> processing can be performed accodring to standardized protocols.  Processing
> is generally limited to small specimens and does not require knowledge of
> anatomy."
>
> This is right from the CAP checklist (ANP.11600)
>
> Roxanne
>
>
>
>
>
> -Original Message-
> From: Histonet Alias 
> To: godsgal...@aol.com
> Cc: histonet@lists.utsouthwestern.edu
> Sent: Mon, 16 Mar 2009 11:13 am
> Subject: Re: [Histonet] grossing
>
> Define presence? If they are able to have access a technical supervisor or
> a Pathologist if they have a question then this may just cover it. We have a
> Pathologist "on call" that can be in within minutes if there are any
> questions.
>
> On Mon, Mar 16, 2009 at 10:55 AM,  wrote:
>
>> I hate to beat a dead horse.but how do you handle non-certified
>> grossing staff.? They all meet the CLIA guidelines, but are they allowed to
>> gross in the evening without the presence of a technical supervisor?
>>
>> And how do you handle salaries for those that process and those that
>> gross.according to the CAP guidelines?
>>
>>
>> Thanks,
>> Roxanne
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> --
> Al Ias HT(ASCP)
> Histology Manager
> Pathology Laboratory
> United States
>
> --
> *A Good Credit Score is 700 or Above. See yours in just 2 easy 
> steps!
> *
>



-- 
Al Ias HT(ASCP)
Histology Manager
Pathology Laboratory
United States
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RE: [Histonet] IHC stainer

[Bower, Jennifer]

I am in a research lab and we are currently testing automated IHC
machines. A couple months ago we had the Bond Max from Leica. The
machine gave me nothing but problems. They said it was because it was a
"demo" unit and the real one would not do that (who knows). The
interesting thing, though, was the quote I received from them. Yes, if
you pay extra you can "open" the system so that you can use any type of
reagent. What they don't tell you is you have to ALSO pay about $1000
for EACH detection system you want to use. For research, that is a
little crazy, I could use a different detection system every day.

Currently we have the Dako Autostainer. It is completely open and I have
had no problems with it. I don't know what was meant by Dako going with
a more "closed" system, the machine we are currently testing is very
open. I like it.

Jennifer Bower

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Sunday, March 15, 2009 10:05 AM
To: 'Pamela Marcum'; 'Greg Dobbin'
Cc: histonet@lists.utsouthwestern.edu; swea...@tvmdl.tamu.edu; 'Ronald
Houston'
Subject: RE: [Histonet] IHC stainer

I have for years worked with animal and human IHC and would have said in
the
past that the Dako open system autostainer was the way to go with the
most
flexibility, I still have the old one and use it all the time because
you
can absolutely use what ever reagent you want and there are way cheaper
ways
to go than buying these from Dako, Ventana, Leica, or BioCare, or the
others.  Now that Dako has gone with the more closed system like Ventana
I
cannot recommend them for open use anymore, a big mistake by Dako in my
opinion.

The Leica Bond instrument may be an option.  They have a research
software
version that allows you to use open containers and fill them with whose
ever
reagents you want, BioCare also has an open system new on the market. 

Sometimes I wonder if in research IHC if it might not be a lot cheaper
and
we would have better control and understand more about what we are doing
if
we went back to manual IHC.  For the Vet. Lab wanting to start their own
IHC
work with not a lot of volume and untrained techs, I would definitely
want
my techs to learn by doing manual staining before I put some instrument
in
there that will teach them more about how to use the computer software
than
anything about IHC, but that is just the opinion of an old school gal
who
sees that there are a lot of people doing critical IHC work who do not
understand what they are doing.

Best regards,

Patsy


Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela
Marcum
Sent: Friday, March 13, 2009 7:50 PM
To: Greg Dobbin
Cc: histonet@lists.utsouthwestern.edu; swea...@tvmdl.tamu.edu; Ronald
Houston
Subject: Re: [Histonet] IHC stainer

Just be sure it is an open system that will allow you to use any primary
antibody you need and secondary antibody not just a secondary kit the
company provides.  You will need to confirm that if you are using a
chicken
primary for instance, you can get and use a secondary link that will
work
with the chicken primary to get your staining reaction.  Be very careful
that it allows you flexibilty and is not designed for clinical standard
usage with no ability to truly allow you the range required for animal
work.
I work with animal and it is hard to find everyhting you need and not
all
"standard" antibodies and systems will work across species.  Good Luck,
Pam
marcum



- Original Message -
From: "Greg Dobbin" 
To: histonet@lists.utsouthwestern.edu, "Ronald Houston"
, swea...@tvmdl.tamu.edu
Sent: Friday, March 13, 2009 8:57:48 PM GMT -05:00 US/Canada Eastern
Subject: RE: [Histonet] IHC stainer

I guess I would have to echo that as well!
Cheers!
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


>>> "Houston, Ronald" 
3/13/2009 4:45:14 PM >>>
Can't speak highly enough of the BondMax form Leica Microsystems. If
cost is an issue and you're looking at what to avoid, IMHO avoid
anything Ventana

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Stephanie Weaver
Sent: Friday, March 13, 2009 3:17 PM
To: histonet post
Subject: [Histonet] IHC stainer

I am in a veterinary diagnostic lab.  In the past we have had very few
requests for IHC and have always sent slides o

[Histonet] paul moylan from dako

[Horn, Hazel V]

Paul Moylan from Dako please contact me.   

 

Hazel Horn

Hazel Horn, HT/HTL (ASCP)

Supervisor of Histology

Arkansas Children's Hospital

1 Children's WaySlot 820

Little Rock, AR   72202

 

phone   501.364.4240

fax501.364.3155

 

visit us on the web at:www.archildrens.org

 

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Re: [Histonet] grossing

[godsgalnow]

Processing, according to CAP? :

"tissue examination limited to description, inking and cutting of the specimen, 
and submission og the entire specimen to histology.? Tissue processing can be 
performed accodring to standardized protocols.? Processing is generally limited 
to small specimens and does not require knowledge of anatomy."

This is right from the CAP checklist (ANP.11600)

Roxanne





-Original Message-
From: Histonet Alias 
To: godsgal...@aol.com
Cc: histonet@lists.utsouthwestern.edu
Sent: Mon, 16 Mar 2009 11:13 am
Subject: Re: [Histonet] grossing


Define presence? If they are able to have access a technical supervisor or a 
Pathologist if they have a question then this may just cover it. We have a 
Pathologist "on call" that can be in within minutes if there are any questions.?


On Mon, Mar 16, 2009 at 10:55 AM,  wrote:

I hate to beat a dead horse.but how do you handle non-certified grossing 
staff.? They all meet the CLIA guidelines, but are they allowed to gross in the 
evening without the presence of a technical supervisor?

And how do you handle salaries for those that process and those that 
gross.according to the CAP guidelines?


Thanks,
Roxanne
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-- 
Al Ias HT(ASCP)
Histology Manager
Pathology Laboratory
United States

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[Histonet] TweakR

[Melissa Mazan]

Hi all,
I'm trying to use Tweak R (Fn14) on mouse lung.  I've got a rabbit 
polyclonal from abcam that supposedly is validated for IHC-paraffin - 
have tried it with and without HIER (citrate) and with and without 
detergents - was worried that as its a transmembrane protein maybe I was 
washing it away.  Has anybody done this on paraffins? If so, can you 
share a protocol? Many thanks - Melissa


--
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cummings School of Veterinary Medicine
200 Westborough Road
North Grafton,MA 01536
tel:508-839-5395
fax:508-839-7903
email:  melissa.ma...@tufts.edu


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Re: [Histonet] grossing

[godsgalnow]

René, my friend

This is my opinion as well and I have stated so, but is there any documentation?

Roxanne


-Original Message-
From: Rene J Buesa 
To: histonet@lists.utsouthwestern.edu; godsgal...@aol.com
Sent: Mon, 16 Mar 2009 11:24 am
Subject: Re: [Histonet] grossing







>From my point of view, shared by all AP and many managers, the answer is NO, 
>they cannot, and should not. Histology samples are too precious to be handled 
>by somebody without the proper training.

René J.

--- On Mon, 3/16/09, godsgal...@aol.com  wrote:


From: godsgal...@aol.com 
Subject: [Histonet] grossing
To: histonet@lists.utsouthwestern.edu
Date: Monday, March 16, 2009, 10:55 AM


I hate to beat a dead horse.but how do you handle non-certified grossing
taff.? They all meet the CLIA guidelines, but are they allowed to gross in the
vening without the presence of a technical supervisor?
And how do you handle salaries for those that process and those that
rossaccording to the CAP guidelines?

hanks,
oxanne
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isto...@lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet







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Re: [Histonet] grossing

[Rene J Buesa]

>From my point of view, shared by all AP and many managers, the answer is NO, 
>they cannot, and should not. Histology samples are too precious to be handled 
>by somebody without the proper training.
René J.

--- On Mon, 3/16/09, godsgal...@aol.com  wrote:

From: godsgal...@aol.com 
Subject: [Histonet] grossing
To: histonet@lists.utsouthwestern.edu
Date: Monday, March 16, 2009, 10:55 AM

I hate to beat a dead horse.but how do you handle non-certified grossing
staff.? They all meet the CLIA guidelines, but are they allowed to gross in the
evening without the presence of a technical supervisor?

And how do you handle salaries for those that process and those that
grossaccording to the CAP guidelines?


Thanks,
Roxanne
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[Histonet] Antibody advice

[Mark Elliott]

Good morning everyone

We are starting a rather large project and I would like to get some input as to 
the best version of antibodies to the following antigens-what are people using 
for the following and where do you get them from. This will be either frozen 
sections or formalin fixed paraffin embedded.

CCR7
S100A8
CXCL13
SMAD6
Thy-1/CD90
HSP47
Prolyl-4-hydroxylase

Thanks for your input.  It is much appreciated

Mark
in cloudy damp Vancouver BC







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Re: [Histonet] grossing

[Histonet Alias]

Define presence? If they are able to have access a technical supervisor or a
Pathologist if they have a question then this may just cover it. We have a
Pathologist "on call" that can be in within minutes if there are any
questions.

On Mon, Mar 16, 2009 at 10:55 AM,  wrote:

> I hate to beat a dead horse.but how do you handle non-certified
> grossing staff.? They all meet the CLIA guidelines, but are they allowed to
> gross in the evening without the presence of a technical supervisor?
>
> And how do you handle salaries for those that process and those that
> grossaccording to the CAP guidelines?
>
>
> Thanks,
> Roxanne
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>



-- 
Al Ias HT(ASCP)
Histology Manager
Pathology Laboratory
United States
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[Histonet] grossing

[godsgalnow]

I hate to beat a dead horse.but how do you handle non-certified grossing 
staff.? They all meet the CLIA guidelines, but are they allowed to gross in the 
evening without the presence of a technical supervisor?

And how do you handle salaries for those that process and those that 
grossaccording to the CAP guidelines?


Thanks,
Roxanne
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RE: [Histonet] decolorizing MMA

[Swain, Frances L]

If you want to remove the MMA you can also use 2-Methoxyethyl Acetate or 
Acetone.  If you want to decolorize like a hematoxylin, yes you can do that 
also.

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfranc...@uams.edu email

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, March 16, 2009 8:28 AM
To: 'Molinari, Betsy'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] decolorizing MMA

Do you mean deplasticize, as in remove the resin?  If that is what you mean,
yes, you can remove mma with xylene, you cannot remove GMA.

Patsy  

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari,
Betsy
Sent: Monday, March 16, 2009 7:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decolorizing MMA

Good morning,

Is it possible to decolorize MMA slides? 

Thanks,

Betsy

 

Betsy Molinari  HT(ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave

MC1-283

Houston,TX 77030-2607

832-355-6524

832-355-6812

 

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RE: [Histonet] decolorizing MMA

[Patsy Ruegg]

Do you mean deplasticize, as in remove the resin?  If that is what you mean,
yes, you can remove mma with xylene, you cannot remove GMA.

Patsy  

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari,
Betsy
Sent: Monday, March 16, 2009 7:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decolorizing MMA

Good morning,

Is it possible to decolorize MMA slides? 

Thanks,

Betsy

 

Betsy Molinari  HT(ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave

MC1-283

Houston,TX 77030-2607

832-355-6524

832-355-6812

 

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[Histonet] RE: decolorizing MMA

[Swain, Frances L]

What do you mean by decolorize?  

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfranc...@uams.edu email
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy
Sent: Monday, March 16, 2009 8:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decolorizing MMA

Good morning,

Is it possible to decolorize MMA slides? 

Thanks,

Betsy

 

Betsy Molinari  HT(ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave

MC1-283

Houston,TX 77030-2607

832-355-6524

832-355-6812

 

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[Histonet] decolorizing MMA

[Molinari, Betsy]

Good morning,

Is it possible to decolorize MMA slides? 

Thanks,

Betsy

 

Betsy Molinari  HT(ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave

MC1-283

Houston,TX 77030-2607

832-355-6524

832-355-6812

 

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RE: [Histonet] Disposal of Formaldehyde

[Bernice Frederick]

We place it in 5 gallon containers and the office of research safety comes
and picks it up and it goes to a waste hauler form there. They pick up
alcohol as well as xylene. If we had the space for a recycler..
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary
Sent: Friday, March 13, 2009 9:27 AM
To: Jessica Piche; histonet
Subject: RE: [Histonet] Disposal of Formaldehyde

First ... even if the local authorities allow this ... it doesn't make
"Green" sense to do it, especially when there are other very workable
alternatives.  The path we chose was to purchase a very simple gravity feed
recycler produced and sold by Creative Waste Solutions (888) 795-8300. Check
them out.
Gary

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica
Piche
Sent: Friday, March 13, 2009 7:11 AM
To: histonet
Subject: [Histonet] Disposal of Formaldehyde

 
Hi All, 
We have a question regarding the disposal of formaldehyde. We were told at
our hospital that a consultant said it was okay to dump formaldehyde down
the drain. I believe they said it was okay to dump 15 gallons or so a day!
We are not to fond of this idea and would like to know what everyone else is
doing. How is everyone disposing of their formaldehyde? We would be
especially interested in what other hospitals in CT are doing. 
Thanks,
Jessica Piche-Grocki, HT(ASCP)
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RE: [Histonet] Dako

[Patsy Ruegg]

Jan,
I understand but you might be surprised at how easy it is to make your own
buffers, find cheaper diluents and protein blocks, plus cheaper detection
reagents that perform as good or better than the ones you are paying so much
for, without changing your protocols.  We have been having this discussion
on the Nsh IHC Resource Group, you can join online at www.ihcrg.org 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
shive...@umn.edu
Sent: Sunday, March 15, 2009 9:21 PM
To: Doug Showers
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dako

Those of us in veterinary medicine diagnostics are really hung out to dry 
with Dako's price increases and push to make us accept the new Flex system. 
In my institution, there is not a separate charge (nor reimbursement) for 
IHC slides done for an autopsy (necropsy) or in-house surgical biopsy; it's 
rolled into the overall pathology charge. (There is no insurance nor 
medicare to fund diagnostic testing for animals.) Yet, IHC is imperative 
for a correct and thorough diagnosis, whether it be for tumors or 
infectious diseases, just like in human medicine. My lab performs about 125 
different IHC tests, all done on Dako autostainers, and most of the tumor 
antibodies are purchased from Dako. Do I want to replace Dako's products 
with other vendors' items and have to redevelop, optimize, and rewrite all 
those SOPs? No Way! But I can't afford Dako anymore and am starting to look 
into what the others have to sell me.

Jan Shivers



On Mar 12 2009, Doug Showers wrote:

>Claire,
> One of the things that irritates me about some companies is their 
> tendency to
> charge for their products based solely on what we get reimbursed rather 
> than
>being happy with making a healthy profit based on their expenses. 
>
>Doug Showers, MS, HT
>Histology Manager
>
>
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles 
> Claire
>
>Sent: Thursday, March 12, 2009 2:23 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: RE: [Histonet] Dako
>
>Why do I keep feeling more and more like these guys are the 'contractors',
> and we are the 'government'. i.e. $50 screwdrivers come to mind... I 
> think we
>need to start looking into these guys like others are looking into the
>pharmaceutical market pricing. They think they have us over a barrel. I say
>we put them in the barrel and pitch them over the falls. (the higher the
> better) How about paying for their goods based on how much we get 
> reimbursed
>by insurance/midicare/etc for the testing using their products. Although I
>have to say my little experience with immunos (on derm frozen sections, no
> less) I was really happy with Biocare. They sent tech specialists to help 
> out
> with protocols and troubleshooting even though we didn't even know if we 
> were
>even going to do the testing at all. Customer service was always great and
> very patient. They even transfered me to a specialist if they didn't know 
> the
>answers! Even their kits were compatable with other antibodies, and I was
>using predilutes. Never failed me, and improved the staining of the other
>brand antibodies when their own kits didn't work.
> Sorry. Tons of stress and migranes the past few days. I might be on the 
> verge
>of going Histo if I'm not careful. Is it Friday yet?
>Claire
>
>
>
>From: histonet-boun...@lists.utsouthwestern.edu on behalf of Thomas Pier
>Sent: Thu 3/12/2009 1:21 PM
>To: godsgal...@aol.com; hor...@archildrens.org; anne.le...@bms.com;
>billodonn...@catholichealth.net; histonet@lists.utsouthwestern.edu;
>jlhow...@yrmc.org
>Subject: RE: [Histonet] Dako
>
>
>
>I've had great luck with Biocare as well.  They've been my primary supplier
>for detection kits and ancillaries since 2003.  I'm probably going to check
>them out for my DAB needs due to Dako DAB+ going from $170 to $400 for 110
>mL.
>
>
>
>
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>
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[Histonet] Permanent Job Openings with Medical Diagnostic Company in New Jersey

[Frederick, David]

Good morning,

I am hiring for several permanent positions with a growing
medical diagnostic company here in Central New Jersey.  My client has
several IMMEDIATE openings for Histology Technicians who have experience
with Gram Staining, Section Cutting and Paraffin Embedding.  Hands-on
experience with Histology Techniques (H&E) is required.

My client is rapidly expanding in 2009 and expects to grow their market
share and revenues in several key diagnostic areas.  These openings are
for immediate direct hire and my client is looking to interview ASAP.
They also have openings for FISH Technologists as well as
Immunohistochemists.  Unfortunately, relocation assistance is not
available.  Though these particular positions are permanent, we do also
place individuals in contract and contract-to-hire positions.

If you are interested, please respond with an updated Word version of
your resume.  If you know anyone who might be a fit for these positions,
please feel free to forward this email along to them.

 If you would like, I will add you to a network of individuals here in
New Jersey that I will email with new openings as they arise as well.
If you are looking for other opportunities, please feel free to reach
out to me as I work with many of the medical device / biotech /
pharmaceutical companies in Central NJ.

Best regards,
David

David Frederick
Aerotek Scientific
371 Hoes Lane, Suite 203, Piscataway, NJ 08854
* Work: 732-447-1187
* Fax: 732-447-1660 / 1661
* E-mail: dfred...@aerotek.com

Who do we know in common?
  




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FW: [Histonet] help with muscle stains

[Ian Montgomery]

Angela,
Martin, T.P. et al 1988, Am. J. Physiol. 255 C43-50
I use this published technique for SD and GPD, works a treat. If you
cannot get the journal let me know and I'll forward the protocol.
Ian.

Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: 16 March 2009 04:10
To: histonet
Subject: [Histonet] help with muscle stains

Would anyone like to share their procedures for Cytochrome oxidase(COX),
Succinic dehydrogenase (SDH) and NADH-TR with me?  I have old, old ones but
they are sketchy. Thanks.

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!




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