RE: [Histonet] Information Systems: Specimen Tracking MiddleWare
Yes, I do agree, that is why I call it a tool for people to use. I think that it is a stereotype to think that histologists are not experienced or knowledgable about computers. There are some histologists who have had a fairly good introduction to computer systems, how computers work, what they can and cannot do, software, applications, interfaces, databases, and have worked with 5 or more LIS systems, barcodes etc. Though admittedly, in my experience this is a rarity. Most of what I have learned about computers, I have gotten from formal classes, but I also have used this knowledge in other arenas, and wish I could use it more in my job. I am just not fortunate enough to have been given the opportunity to have much influence on the processes, or the computer systems. I think that many who have been promoted into management simply also accept this stereotype that histologists know only technical information, and so we are not consulted, though we do the work everyday.I wish that you could come to our lab and educate those who have been given this authority! I would love to have a computer geek come to our lab and inform us of what is available to help us to our jobs better. Joelle Weaver HTL (ASCP) From: m...@pathview.com To: joellewea...@hotmail.com; jel...@yumaregional.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Information Systems: Specimen Tracking MiddleWare Date: Thu, 26 Mar 2009 18:14:21 -0500 People are always at the forefront. Someone has to build that new tool, or come up with some new process or whatever. That's why before we do any installation of our software, we spend what probably amounts to 100 to 200 hours interviewing and watching each clerk, PA, histotech, secretary, cytotech, and pathologist and THEN we propose how we would install and tailor our software. By the way, at the end of that analysis, people are usually pretty tired of hearing me ask 'why do you do that', but guess what -- you are way, way more likely to get 'buy in' from the staff. That tech you spoke to at 3 a.m. remembers that some computer geek took the time and effort and asked them how they would do things better. ...but let me address a real world issue. I am not versed in the technologies of many aspects of the AP/Cytology department (you'll never hear me speak on subjects of which stainer is better for instance), but I do know a few things about work flow and computerization. I like to illustrate via example, so let's try this one: In the real world, a histotechnologist may have only worked in let's say 3 or 4different labs in their life, and perhaps only 1 or 2 different computer systems. With that background, how are they supposed to know what's possible or not possible to do with computer technology. Personally, I think it's the job of the LIS vendor to work TOGETHER with the histotechnologist and other department personnel to come up with better solutions. In this example, each side has knowledge and experience that needs to be conveyed to the other. When that communication occurs, magic happens. Barcodes are not the magic. It's how you use those barcodes in your work flow. It's always about the people. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, March 26, 2009 4:53 PM To: jel...@yumaregional.org; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking MiddleWare Well, I can't speak for everyone of course, but I know in the program that I am affiliate with that we stress, if not require, thinking beyond the manual methods. In fact, I really see an in depth understanding of basic manual histology methods as only a beginning point to how I want the future histologists to think and apply their technical knowledge.I encourage this at every opportunity myself, in every course. Crtical thinking skills, process thinking and the ability to see how our function fits into total laboratory and diagnostic patient services is stressed. I see it as imperative that this is incorporated into training in formal programs and within the lab. We cannot afford to not further this trend. I really don't see any other alternative really. If you look at newer instrumentation, it really is little more than a computer with software and application interfaces connected to the mechanics that perform the tasks of histology. Technology is really just another tool at our disposal to perform our jobs better. We in histology, are due to begin to merge and become cohesive with the totality of healthcare delivery and to begin to operate in such a parallel manner. I believe that the emerging histologist will be better armed with a broader educational background that provides this insight,due
[Histonet] UK only.
One of the departments in the University runs a class each year where they have the student's process, section and stain a batch of tissues. The microtome's they use have come from Noah's histology lab on the Ark. The mechanical workshop tries to keep them functional but they are really beyond economic repair. Obviously, re-equipping with new for a once in the year class is not an option and carrying microtome's around the campus is definitely not the favoured option. Does anyone know of a supplier of refurbished microtomes in the UK? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Information Systems: Specimen Tracking MiddleWare
Yea, I probably didn't communicate quite clearly enough, but I didn't want to elaborate too much as I suspect some people may be getting tired of hearing me talk. .but yes, invariably in every lab I run into, I find at least one person in each area who is more knowledgeable about computers than others and the good news, is that I suspect that trend will increase. Remember a lot of people in this area didn't grow up with computers. The newer generations are quite different. Michael Mihalik http://www.pathview.com/ PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 From: joelle weaver [mailto:joellewea...@hotmail.com] Sent: Friday, March 27, 2009 4:48 AM To: m...@pathview.com; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking MiddleWare Yes, I do agree, that is why I call it a tool for people to use. I think that it is a stereotype to think that histologists are not experienced or knowledgable about computers. There are some histologists who have had a fairly good introduction to computer systems, how computers work, what they can and cannot do, software, applications, interfaces, databases, and have worked with 5 or more LIS systems, barcodes etc. Though admittedly, in my experience this is a rarity. Most of what I have learned about computers, I have gotten from formal classes, but I also have used this knowledge in other arenas, and wish I could use it more in my job. I am just not fortunate enough to have been given the opportunity to have much influence on the processes, or the computer systems. I think that many who have been promoted into management simply also accept this stereotype that histologists know only technical information, and so we are not consulted, though we do the work everyday.I wish that you could come to our lab and educate those who have been given this authority! I would love to have a computer geek come to our lab and inform us of what is available to help us to our jobs better. Joelle Weaver HTL (ASCP) From: m...@pathview.com To: joellewea...@hotmail.com; jel...@yumaregional.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Information Systems: Specimen Tracking MiddleWare Date: Thu, 26 Mar 2009 18:14:21 -0500 People are always at the forefront. Someone has to build that new tool, or come up with some new process or whatever. That's why before we do any installation of our software, we spend what probably amounts to 100 to 200 hours interviewing and watching each clerk, PA, histotech, secretary, cytotech, and pathologist and THEN we propose how we would install and tailor our software. By the way, at the end of that analysis, people are usually pretty tired of hearing me ask 'why do you do that', but guess what -- you are way, way more likely to get 'buy in' from the staff. That tech you spoke to at 3 a.m. remembers that some computer geek took the time and effort and asked them how they would do things better. ...but let me address a real world issue. I am not versed in the technologies of many aspects of the AP/Cytology department (you'll never hear me speak on subjects of which stainer is better for instance), but I do know a few things about work flow and computerization. I like to illustrate via example, so let's try this one: In the real world, a histotechnologist may have only worked in let's say 3 or 4different labs in their life, and perhaps only 1 or 2 different computer systems. With that background, how are they supposed to know what's possible or not possible to do with computer technology. Personally, I think it's the job of the LIS vendor to work TOGETHER with the histotechnologist and other department personnel to come up with better solutions. In this example, each side has knowledge and experience that needs to be conveyed to the other. When that communication occurs, magic happens. Barcodes are not the magic. It's how you use those barcodes in your work flow. It's always about the people. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, March 26, 2009 4:53 PM To: jel...@yumaregional.org; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking MiddleWare Well, I can't speak for everyone of course, but I know in the program that I am affiliate with that we stress, if not require, thinking beyond the manual methods. In fact, I really see an in depth understanding of basic manual histology methods as only a beginning point to how I want the future histologists to think and apply their technical knowledge.I encourage this at every opportunity myself, in every course. Crtical thinking skills, process thinking and the ability to see how our function fits into total laboratory
RE: [Histonet] Information Systems: Specimen Tracking MiddleWare
I do agree that computers are tools that are indeed an asset to anatomic path laboratories. Michael I applaud you for your efforts in getting the staff and engaging them. This is the basis of my entire theory, in order to create efficiencies within histology that there are 3 distinct area of the process Histology, Transcription, and Pathologist. Unlike the clinical laboratory we are not a straight test result type of methodology, rather a fair straight forward process that has inter-connected components. Those components have for so long relied o the fact of internal checks and balances, but with the explosion that has happened within AP in the last 10-15 years we are seeing those checks and balances begin to have cracks and stress points. I would applaud anyone that takes advance courses in anything, but I would caution an IT person looking at Anatomic Pathology that does not have the clinical background that is necessary to see the cracks and stress points. I use PowerPath as my LIS and as the University of Washington our facility has made strides in stream lining and innovation with our LIS,, but I am open to help anyone that is looking to get information on this subject. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 (Fax: (928) 336-7319 *Email: jel...@yumaregional.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Friday, March 27, 2009 2:48 AM To: m...@pathview.com; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking MiddleWare Yes, I do agree, that is why I call it a tool for people to use. I think that it is a stereotype to think that histologists are not experienced or knowledgable about computers. There are some histologists who have had a fairly good introduction to computer systems, how computers work, what they can and cannot do, software, applications, interfaces, databases, and have worked with 5 or more LIS systems, barcodes etc. Though admittedly, in my experience this is a rarity. Most of what I have learned about computers, I have gotten from formal classes, but I also have used this knowledge in other arenas, and wish I could use it more in my job. I am just not fortunate enough to have been given the opportunity to have much influence on the processes, or the computer systems. I think that many who have been promoted into management simply also accept this stereotype that histologists know only technical information, and so we are not consulted, though we do the work everyday.I wish that you could come to our lab and educate those who have been given this authority! I would love to have a computer geek come to our lab and inform us of what is available to help us to our jobs better. Joelle Weaver HTL (ASCP) From: m...@pathview.com To: joellewea...@hotmail.com; jel...@yumaregional.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Information Systems: Specimen Tracking MiddleWare Date: Thu, 26 Mar 2009 18:14:21 -0500 People are always at the forefront. Someone has to build that new tool, or come up with some new process or whatever. That's why before we do any installation of our software, we spend what probably amounts to 100 to 200 hours interviewing and watching each clerk, PA, histotech, secretary, cytotech, and pathologist and THEN we propose how we would install and tailor our software. By the way, at the end of that analysis, people are usually pretty tired of hearing me ask 'why do you do that', but guess what -- you are way, way more likely to get 'buy in' from the staff. That tech you spoke to at 3 a.m. remembers that some computer geek took the time and effort and asked them how they would do things better. ...but let me address a real world issue. I am not versed in the technologies of many aspects of the AP/Cytology department (you'll never hear me speak on subjects of which stainer is better for instance), but I do know a few things about work flow and computerization. I like to illustrate via example, so let's try this one: In the real world, a histotechnologist may have only worked in let's say 3 or 4different labs in their life, and perhaps only 1 or 2 different computer systems. With that background, how are they supposed to know what's possible or not possible to do with computer technology. Personally, I think it's the job of the LIS vendor to work TOGETHER with the histotechnologist and other department personnel to come up with better solutions. In this example, each side has knowledge and experience that needs to be conveyed to the other. When that communication occurs, magic happens. Barcodes are not the magic. It's how you use those barcodes in your work flow. It's always about the people.
RE: [Histonet] Re: Different between Richard Allan and Harris which one is better for IHC
I use Mayer's Hematoxlyin for 30-45 secs. Wash for 2 minutes, blue in Ammonia Water, wash for 5 minutes, dehydrate clear and mount if it is DAB. If it is AEC I do the same except I rinse in distilled water before mounting with aqueous mount. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, March 27, 2009 9:23 AM To: ooi.ting.h...@nhc.com.sg; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Different between Richard Allan and Harris which one is better for IHC Hi all, I am adding my question to the below one. Which is better to use for IHC: Mayer's or just hematoxylin? Thanks in advance, Naira Message: 15 Date: Fri, 27 Mar 2009 09:50:42 +0800 From: ooi.ting.h...@nhc.com.sg Subject: [Histonet] Different between Richard Allan and Harris Hematoxylin Hi, I am interested to know what is the different between different brands of hematoxylin especially for Richard Allan and Harris. I am glad if there is any advice or suggestion on the choosing of hematoxylin. Welcome any suggestion of webpage that may tell the differences too. I am dealing with plastic resin section and doing a normal hematoxylin and eosin staining. Your advice is appreciated. Thank you very much! Regards, Ooi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Agar removing for fluorescent stainings
Hey everybody! Today I tried to do a fluorescent staining on cryosectionned tissue embedded in agar. I didn't see a problem in the presence of the agar untill I analyzed the slides under the microscope... The agar had absorbed my fluorescent dy!! Does anyone know who to remove the agar where the tissue is embedded in to make it possible to do cryosections? I tried with 37°C PBS to 'melt' it and rinse it away but kept sticking on the slide.. Any advice? Thanks alot for your help! Greetings, Barbara Verstraeten, Drs. Vertebrate Morpholgy and Developmental Biology Department of Biology Ghent University K.L. Ledeganckstraat 35 B-9000 Ghent Belgium tel: ++32/(0)9 264 52 31 fax: ++32/(0)9 264 53 44 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Please help! In dire need of user manuals
OUCH JTT - Original Message - From: fro...@bitstream.net To: Jennifer Anderson jander...@halozyme.com Cc: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, March 26, 2009 9:50 PM Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite Flaming Friday yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has purchased all used equipment. What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great RD expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you won't hold your breath for that. My Second Question: Are you crazy? From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you will repay the favor, if at all possible. I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford the demon vendor Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN Hello Everyone. I our lab we've purchased all used equipment. None of these instruments came with a user's manual. I am in need of a manual (hard-copy or PDF) for the following: Sakura Tissue-Tek VIP 3000 Leica Jung Histo Embedder Microm HM335E Microtome I do realize that requesting a copy of these is a lot to ask of someone. It takes a lot of time to copy a 50-page manual. I'll repay the favor, if at all possible. I'm hoping that a vendor may raise their hand and offer a copy? I won't hold my breath for that... Thanks everyone. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11404 Sorrento Valley Road San Diego, CA 92121 858-704-8333 jander...@halozyme.com mailto:jander...@halozyme.com The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Please help! In dire need of user manuals
The response below is completely and utterly disrespectful and downright rude Steven Mello,HT(ASCP) Anatomical Pathology Manager From: jnoc...@satx.rr.com To: fro...@bitstream.net; jander...@halozyme.com Date: Fri, 27 Mar 2009 10:52:53 -0500 Subject: Re: [Histonet] Please help! In dire need of user manuals CC: histonet@lists.utsouthwestern.edu OUCH JTT - Original Message - From: fro...@bitstream.net To: Jennifer Anderson jander...@halozyme.com Cc: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, March 26, 2009 9:50 PM Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite Flaming Friday yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has purchased all used equipment. What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great RD expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you won't hold your breath for that. My Second Question: Are you crazy? From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you will repay the favor, if at all possible. I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford the demon vendor Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN Hello Everyone. I our lab we've purchased all used equipment. None of these instruments came with a user's manual. I am in need of a manual (hard-copy or PDF) for the following: Sakura Tissue-Tek VIP 3000 Leica Jung Histo Embedder Microm HM335E Microtome I do realize that requesting a copy of these is a lot to ask of someone. It takes a lot of time to copy a 50-page manual. I'll repay the favor, if at all possible. I'm hoping that a vendor may raise their hand and offer a copy? I won't hold my breath for that... Thanks everyone. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11404 Sorrento Valley Road San Diego, CA 92121 858-704-8333 jander...@halozyme.com mailto:jander...@halozyme.com The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by
RE: [Histonet] Please help! In dire need of user manuals
Double ouch! Somebody has their panties in a bunch today! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, March 27, 2009 11:53 AM To: fro...@bitstream.net; Jennifer Anderson Cc: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals OUCH JTT - Original Message - From: fro...@bitstream.net To: Jennifer Anderson jander...@halozyme.com Cc: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, March 26, 2009 9:50 PM Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite Flaming Friday yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has purchased all used equipment. What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great RD expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you won't hold your breath for that. My Second Question: Are you crazy? From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you will repay the favor, if at all possible. I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford the demon vendor Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN Hello Everyone. I our lab we've purchased all used equipment. None of these instruments came with a user's manual. I am in need of a manual (hard-copy or PDF) for the following: Sakura Tissue-Tek VIP 3000 Leica Jung Histo Embedder Microm HM335E Microtome I do realize that requesting a copy of these is a lot to ask of someone. It takes a lot of time to copy a 50-page manual. I'll repay the favor, if at all possible. I'm hoping that a vendor may raise their hand and offer a copy? I won't hold my breath for that... Thanks everyone. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11404 Sorrento Valley Road San Diego, CA 92121 858-704-8333 jander...@halozyme.com mailto:jander...@halozyme.com The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error,
[Histonet] tissues and static
has anyone had any trouble when embedding little specimens they float to the sides of the molds like they are charged. it happens sometimes with our little core bxs. thanks, everyone have a good weekend! anita dudley providence hosp mobile alabama _ Quick access to Windows Live and your favorite MSN content with Internet Explorer 8. http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Please help! In dire need of user manuals
The secret of life is honesty and fair dealing. If you can fake that, you've got it made. - Groucho Marx, Double-dog ouch. Nuff said, I'm backing out now, William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, March 27, 2009 11:01 AM To: Joe Nocito; fro...@bitstream.net; Jennifer Anderson Cc: Histonet Subject: RE: [Histonet] Please help! In dire need of user manuals Double ouch! Somebody has their panties in a bunch today! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, March 27, 2009 11:53 AM To: fro...@bitstream.net; Jennifer Anderson Cc: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals OUCH JTT - Original Message - From: fro...@bitstream.net To: Jennifer Anderson jander...@halozyme.com Cc: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, March 26, 2009 9:50 PM Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite Flaming Friday yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has purchased all used equipment. What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great RD expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you won't hold your breath for that. My Second Question: Are you crazy? From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you will repay the favor, if at all possible. I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford the demon vendor Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN Hello Everyone. I our lab we've purchased all used equipment. None of these instruments came with a user's manual. I am in need of a manual (hard-copy or PDF) for the following: Sakura Tissue-Tek VIP 3000 Leica Jung Histo Embedder Microm HM335E Microtome I do realize that requesting a copy of these is a lot to ask of someone. It takes a lot of time to copy a 50-page manual. I'll repay the favor, if at all possible. I'm hoping that a vendor may raise their hand and offer a copy? I won't hold my breath for that... Thanks everyone. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11404 Sorrento Valley Road San Diego, CA 92121 858-704-8333
[Histonet] plastic slides for sale
For those who prepare specimens for ground histology we have plastic slides available. Since I am closing my lab we will no longer be selling custom plastic slides. We are closing our stock at half price. 2 x 3 x .030 clear800 slides 2 x 3 x .060 clear64 slides 2 x 3 x .060 white769 slides 2 x 2 x .060 clear88 slides 2 x 2 x .060 white 112 slides higher quality plastic slides 2 x 3 x .030 clear346 slides Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Staining vs. paraffin wax problems
I would suggest you send your recycled xylene for testing; the manufacturer of your recycler should be able to recommend several. I suspect water in the xylene (I have seen it cause this issue on the processor) or inadequate drying of the slides before deparaffinization. Any water droplets left on the slide will not be removed by the xylene on the stainer leaving tiny round areas in the tissue that still have paraffin in them. The hematoxylin and eosin will not fully stain these areas, but the xylene at the end of the HE stain will remove this residual wax. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 214-237-1680 214-422-3083 Mobile 214-237-1706 FAX To learn more about ProPath, please visit http://www.ProPathLab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jason McGough Sent: Friday, March 27, 2009 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staining vs. paraffin wax problems We are experiencing difficulties with our HE staining and other Special Stains. After the slides are stained the edges of the tissue look fragmented/chattered. Our pathologists also thought the sections looked like they might be too thick but we checked all of our microtomes for thickness settings and all are set at 4 micrometers. There seems to be several blotches of light staining throughout the tissue. This seems to be only on our small biopsies. We use McCormick Paraplast Extra for paraffin wax. Another bit information is that we recycle all of our xylene. Is this a deparaffinization, processing, or a staining issue? Any help is appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Please help! In dire need of user manuals
Flaming? This is total burn out! Very inappropriate. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor 183 E. 8th Ave. Chico, CA 95926 530-891-6244 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of fro...@bitstream.net Sent: Thursday, March 26, 2009 7:51 PM To: Jennifer Anderson Cc: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite Flaming Friday yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has purchased all used equipment. What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great RD expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you won't hold your breath for that. My Second Question: Are you crazy? From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you will repay the favor, if at all possible. I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford the demon vendor Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN Hello Everyone. I our lab we've purchased all used equipment. None of these instruments came with a user's manual. I am in need of a manual (hard-copy or PDF) for the following: Sakura Tissue-Tek VIP 3000 Leica Jung Histo Embedder Microm HM335E Microtome I do realize that requesting a copy of these is a lot to ask of someone. It takes a lot of time to copy a 50-page manual. I'll repay the favor, if at all possible. I'm hoping that a vendor may raise their hand and offer a copy? I won't hold my breath for that... Thanks everyone. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11404 Sorrento Valley Road San Diego, CA 92121 858-704-8333 jander...@halozyme.com mailto:jander...@halozyme.com The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. ___ Histonet mailing list
[Histonet] ? About fixation of excessively bloody tissues
Does anyone out there know of a reference that states or discusses tissues that a covered with a large amount of excess blood have problems with fixation. I have looked in the reference materials I have here in the lab, however I cannot find anything to support my complaints to a client that state entire mouse organs (whole liver, spleen, lung, heart...) covered in blood thrown into onecassette with to little formalin will not be well preserved. I know garbage in garbage out - I am trying to get rid of the garbage. Thanks in advance for the help. Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Um...Ok!
Wow. Sorry to say it but this whole manual comeback thing is pretty crazy. Talk about panties in a bunch. How about switching to decaf! Histonet is a great resorce for individuals to come for help and/or discussions for ceratin things including answers, comments and well... I would think sometimes manuals and things of that sort. We really should not have to be intimidated or TOTALLY insulted by others. How about next time...try not opening the email if your going to respond in such a manner. Hopefully from now on you wont check your e.mail after getting upset at something else, then directing it towards your keyboard in RUDE words on histonet. JD HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TGIF-Permanent Histotech Job In Central Wisconsin
TGIF! I have an opportunity for a full time/permanent histotechnologist/histotechnician in *Central Wisconsin.* Please forward this to anyone you know who may seem fit for this position, and if we place on of your referrals in a position you will earn a $1000 referral bonus. If you are interested the first step would be to please forward your resume to: aly...@alliedsearchpartners.com Exprience and Education: High school graduate with a background in biology and chemistry and successful completion of an accredited program in histologic technique required. College coursework desirable. HT ASCP registry or eligible. CPR certified. Minimum one year of experience in a Histology Lab preferred. Job Description: The Histology Technician will assist the physician during the grossing of specimens specifically for the MOHS procedures in the Dermatology Department. The Tech will process, embed, cut and stain tissue specimens so a quality microscopic slide can be produced for diagnosis by a physician/pathologist. Benefits: Generous compensation and benefits package that includes a 401(k) with immediate vesting. Shift: Monday-Friday, 8am-4:30pm. -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] more plastic slides
found some more plastic slides 2 x 4 x 060 clear164 slides These we used to true the head on the Exakt grinder. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TMA tape sectioning system
Does anyone out there remember who had the nifty sectioning tape for TMA's? Or does anyone use it? The vendor was at the NSH meeting last fall. Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Trypsin versus other AR-s
Hi Dears, It is Friday, but not a weekend yet:-):-( I just got a request from my PI to figure out: 1. How often now day's people use Trypsin (EDTA, Proteinase K or E) as an Antigen Retrieval for FFPE. 2. Why or is the Citrate Buffer pH6 more usable??? 3. Is Trypsin very old technique? Any educational feedback is appreciated, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Trypsin versus other AR-s
I use Trypsin only as a last resort. I use Pepsin because it is more gentle (does not chew up the sections). I do not use Proteinase K unless I absolutely have to and for just a few minutes as it will eat the sections off of the slides. If I use Citrate Buffer I heat it up in the microwave first then quickly put the slides in it cover it and let it sit on the bench top until cool about 20 minutes. As to why we use Citrate buffer there has been several discussions about that on the histonet. You might want to search the archieves and see what others have had to say. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, March 27, 2009 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trypsin versus other AR-s Hi Dears, It is Friday, but not a weekend yet:-):-( I just got a request from my PI to figure out: 1. How often now day's people use Trypsin (EDTA, Proteinase K or E) as an Antigen Retrieval for FFPE. 2. Why or is the Citrate Buffer pH6 more usable??? 3. Is Trypsin very old technique? Any educational feedback is appreciated, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TMA tape sectioning system
The tape is made by instrumedics and the system is the cryojane tape transfer system. Christie From: b-freder...@northwestern.edu To: histonet@lists.utsouthwestern.edu Date: Fri, 27 Mar 2009 13:39:06 -0500 Subject: [Histonet] TMA tape sectioning system Does anyone out there remember who had the nifty sectioning tape for TMA's? Or does anyone use it? The vendor was at the NSH meeting last fall. Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Please help! In dire need of user manuals
I, for one, love how he begins all politely with hopefully not too offensive to those that may be sensitive to such replies before getting straight to Are you crazy? not even three paragraphs later. This is classic trolling at its finest... congratulations Ford! I'll be sure to keep your rant in my inbox so that the next time a pet-peeve of mine comes across the list (i,e: please unsubscribe me!) I can refer to yours as a good example of how not to reply to such a huge, helpful list. Lol... fro...@bitstream.net wrote: Dear Jennifer and any other Histonetters that are of like mind, It's not quite Flaming Friday yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has “purchased all used equipment”. What vendor did you purchase from that did not provide operator’s manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let’s make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great RD expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor’s expense for the shipping handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you “won’t hold your breath for that”. My Second Question: Are you crazy? From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you “will repay the favor, if at all possible”. I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year’s supply of PH20 when it is released to the public... all at no charge, including shipping handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford “the demon vendor” Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN Hello Everyone. I our lab we've purchased all used equipment. None of these instruments came with a user's manual. I am in need of a manual (hard-copy or PDF) for the following: Sakura Tissue-Tek VIP 3000 Leica Jung Histo Embedder Microm HM335E Microtome I do realize that requesting a copy of these is a lot to ask of someone. It takes a lot of time to copy a 50-page manual. I'll repay the favor, if at all possible. I'm hoping that a vendor may raise their hand and offer a copy? I won't hold my breath for that... Thanks everyone. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11404 Sorrento Valley Road San Diego, CA 92121 858-704-8333 jander...@halozyme.com mailto:jander...@halozyme.com The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. ___ Histonet mailing
Re: [Histonet] TMA tape sectioning system
There is also a paraffin transfer system now it is all from Leica. You can use the paraffin system with the slides for frozen sections and it works well if you don't have the money for the whole Cryo Jane system. They purchased the company that bought Instrumedics last year. It was under McCormick Scientific prior to the Leica buy out. Pam Marcum - Original Message - From: CHRISTIE GOWAN christiego...@msn.com To: b-freder...@northwestern.edu, histonet@lists.utsouthwestern.edu Sent: Friday, March 27, 2009 4:21:31 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] TMA tape sectioning system The tape is made by instrumedics and the system is the cryojane tape transfer system. Christie From: b-freder...@northwestern.edu To: histonet@lists.utsouthwestern.edu Date: Fri, 27 Mar 2009 13:39:06 -0500 Subject: [Histonet] TMA tape sectioning system Does anyone out there remember who had the nifty sectioning tape for TMA's? Or does anyone use it? The vendor was at the NSH meeting last fall. Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: difference in hematoxylins
So, there are tons of hematoxylin recipes out there, and many, I find, are very similar in their staining properties. Yes, Richard Allen manufactures a modification of Harris' Hematoxylin, which is essentially Harris' recipe without the mercuric oxide. I don't know how long Richard Allen's variety of this stain keeps, but the traditional Harris' Hematoxylin was only good for a few months. I typically go with either Delafield's, Mayer's (Lillie Mod.) or Ehrlich's Hematoxylin for general HE staining. These solutions can be used for progressive or regressive techniques and keep for several years. My advice would be to sample a few bottles and see which one works best for you. Or, you could give a hand at making your own. Hematoxylin is very easy to make from essentially hematoxylin, alum (potassium or ammonium), glycerin, sodium iodate, distilled water, alcohol and acetic acid. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hours of operation
I was wondering how many Histo labs in Australia are working on a 24 hour system. What have they seen as the advantages but more importantly the disadvantages with such a system. P ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Please help! In dire need of user manuals
I thought the rant was pretty funny. Just repeat to yourself it's an email list, I should really just relax. Emily -- prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
