[Histonet] RE: Research Genetics
Research Genetics has merged a couple of time the last few years. They are now Open Biosystems in Huntsville, Al pho 888 412 2225. They do not list the Research Genetics on the Web but last I knew products were still available. You can call them to get current catalog number and prices. Donna Reynolds HT (ASCP) Chief Histology Laboratory U. T. M.D. Cancer Center Cancer Biology Core Immunohisto Lab 713-792-8106 Message: 2 Date: Mon, 15 Jun 2009 14:52:39 -0400 From: Joanne Mauger mau...@email.chop.edu Subject: [Histonet] Re: research genetics/phoenix biotech To: jluis.pala...@icman.csic.es,histonet@lists.utsouthwestern.edu Message-ID: 4a36603702317...@email.chop.edu Content-Type: text/plain; charset=US-ASCII Hi everyone, I am unable to find this company anymore. Does anyone know if old research genetics products are still being sold? I don't want Invitrogen- they did away with the product I want-MicroHyb. Thanks, Jo Mauger -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Inactivation of DAB
We have started doing routine immunohistochemistry on our stainer and have a large waste container that includes diaminobenzidine (DAB). We would like to inactivate it so we don't have to pay to have the waste removed. Can anyone help me? What do people use to inactivate their DAB? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for NYU contact
Just wondering if anyone can give me contact information for someone in the Neuropath Lab at NYU? Thanks Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Inactivation of DAB
Hi Peggy, You wrote: We have started doing routine immunohistochemistry on our stainer and have a large waste container that includes diaminobenzidine (DAB). We would like to inactivate it so we don't have to pay to have the waste removed. Can anyone help me? What do people use to inactivate their DAB? May I suggest that you access the histonet archives where you will be able to see all the previous discussions on this topic. The responses were many and varied depending on different lab types and locations. Good luck with your search. Respectfully, Christie Gowan Surgical Pathology University of Alabama Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] source for rat anti mouse brdu?
I have not done a brdu on mouse in five years. Used Harlan but they are not selling that anymore. I used a procedure using rat anti mouse brdu primary, rabbiot anti rat mouse adsorbed secondary, then ABC/DAB. Anyone have any idea on a good source? I used biocompare and found stuff but would like to hear comments. Also anyone use conjugated brdu? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histolgy jobs
I am looking for histology job, preferably a day shift !! I am registered ASCP ! I live in Dallas have a job at this time ! Do you know of any jobs at UTSW ?? I would surely appreciate it, if you would let me know. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Endogenous GFP frozen sectioning
I have been asked to section 10 dpn mouse ovaries embedded in OCTfor endogenous GFP. These are very, very tiny. The investigator would lik= e to see the endogenous GFP from the sections on the slide and asked for = 5 microns. My thought is they are unlikely to see the GFP at 5 microns an= d 10 would be better. What section thickness would be best for this? Thanks, Donna Donna J. Emge, Research Histology and Phen= otyping Laboratory Manager Northwestern University 710 N. Fairbanks= Ct., Olson Bldg. 8-335 312-503-2679 [1]d-e...@northwestern.edu References 1. 3Dmailto:d-e...@north___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 67, Issue 17
From: histonet-requ...@lists.utsouthwestern.edu Reply-To: histonet@lists.utsouthwestern.edu Date: Thu, 18 Jun 2009 03:12:15 +1000 To: histonet@lists.utsouthwestern.edu Conversation: Histonet Digest, Vol 67, Issue 17 Subject: Histonet Digest, Vol 67, Issue 17 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. PR and ER staining in dog uterus (Karen Doty) 2. Re: Inactivation of DAB (Sherwood, Margaret ) 3. RE: Research Genetics (Reynolds,Donna M) 4. Looking for NYU contact (Angela Bitting) 5. Re: Re: Inactivation of DAB (Lynette Pavelich) 6. RE: Re: Inactivation of DAB (CHRISTIE GOWAN) -- Message: 1 Date: Tue, 16 Jun 2009 15:10:06 -0500 From: Karen Doty k...@live.com Subject: [Histonet] PR and ER staining in dog uterus To: histonet@lists.utsouthwestern.edu Message-ID: col104-w3115715881a9aeb048fa6ce4...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Hi, Folks, We are starting to work on a project that requires PR and ER staining in dog uterus. I'm hoping someone will share some tips such as which antibodies work well in dog (clone, manufacturer), and any specific special procedures that might help, such as which antigen retrieval methods work best. I've tried a couple antibodies already without good results in dog although they worked very well in a mouse control. All suggestions and advice is appreciated. Thanks very much. Karen Doty Dept. of Veterinary Biosciences University of Illinois _ Insert movie times and more without leaving Hotmail®. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori al_QuickAdd_062009 -- Message: 2 Date: Wed, 17 Jun 2009 09:30:55 -0400 From: Sherwood, Margaret msherw...@partners.org Subject: [Histonet] Re: Inactivation of DAB To: histonet histonet@lists.utsouthwestern.edu Message-ID: 073ae2bea1c2ba4a8837ab6c4b943d9703e23...@phsxmb30.partners.org Content-Type: text/plain; charset=us-ascii We have started doing routine immunohistochemistry on our stainer and have a large waste container that includes diaminobenzidine (DAB). We would like to inactivate it so we don't have to pay to have the waste removed. Can anyone help me? What do people use to inactivate their DAB? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. -- Message: 3 Date: Wed, 17 Jun 2009 08:33:15 -0500 From: Reynolds,Donna M dreyn...@mdanderson.org Subject: [Histonet] RE: Research Genetics To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 785bbf0c5f49ce41ba74460a43a08f021449221...@dcpwvmbxc0vs3.mdanderson.edu Content-Type: text/plain; charset=us-ascii Research Genetics has merged a couple of time the last few years. They are now Open Biosystems in Huntsville, Al pho 888 412 2225. They do not list the Research Genetics on the Web but last I knew products were still available. You can call them to get current catalog number and prices. Donna Reynolds HT (ASCP) Chief Histology Laboratory U. T. M.D. Cancer Center Cancer Biology Core Immunohisto Lab 713-792-8106 Message: 2 Date: Mon, 15 Jun 2009 14:52:39 -0400 From: Joanne Mauger mau...@email.chop.edu Subject: [Histonet] Re: research genetics/phoenix biotech To: jluis.pala...@icman.csic.es,histonet@lists.utsouthwestern.edu Message-ID: 4a36603702317...@email.chop.edu Content-Type: text/plain; charset=US-ASCII Hi everyone, I am unable to find this company anymore. Does anyone know if old research genetics products are still being sold? I don't want Invitrogen- they did away with the product I want-MicroHyb. Thanks, Jo Mauger -- -- Message: 4 Date: Wed,
[Histonet] TTF-1
Hi everyone, Has anyone had problems with TTF-1 not staining Bouin's fixed tissue. We stain ours on the DAKO Autostainer with Envision+ Dual Link HRP System. We use DAKO pH9 target retrieval in the pressure cooker, and use a 1:50 dilution of DAKO TTF-1 (M3575). Ellen Yee Diagnostic Pathology Medical Group, Inc. Sacramento, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
