[Histonet] myelin basic protein
Hi everbody, I'm currently looking for an antibody that stains myelin in mouse brains (no animals with EAE). Does anyone have experences with antibodies against MBP, MAG or other myelin proteins in mouse brains and can give me a suggestion, which one is the best to order for paraffin embedded tissue? Thanks a lot Frauke ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Can I get some help with Survivin ab.
Hallo Everyone, For those of you who have used this antibody on FFPE human breast tissue, can you provide me with a protocol and antibody information? I have tried antibodies from Cell Signaling, Spring and two from Biocare, however I am not getting the results that are the same as in the publications. Any help/response would be greatly appreciated. thanks you and have a very good day. Dusko Trajkovic DSRD Scientist Pfizer Inc La Jolla 858-638-6202 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Special stainers
What stainer is everyone liking the best? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] human dendritic cell staining
Hello! I am trying to stain dendritic cells (DCs) - from the immune system (not the brain) - in formalin/paraffin sections. I've heard that fascin is a commonly used marker but I don't think it's very specific - I see some endothelial cell staining too. Looking online, I've also found other possible markers, namely CD11c, CD68, and 33D1. Having never done this staining before, 1. Which marker for DC is routinely use in Immunohistochem (IHC)? 2. Are there other possible markers that are specific to DCs for IHC? Do these markers stain only DCs or also monocytes, macrophages, etc? 3. Also, if you have done staining for any of these markers, could you suggest antibodies and/or protocols? Thank you! Deepali Dhar Yale School of Medicine 2012 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Special stainers
The Artisan (DAKO) is the best. Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Tuesday, July 14, 2009 9:13 AM To: histonet Subject:[Histonet] Special stainers What stainer is everyone liking the best? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] TUNEL_FFPE_very_long OT_Delete_if_uninterested
Hi Jerry, I would never try to persuade anyone. I'm no smarter than the next lab worker trying to make sense of this and science biology in general. But this is how I see TUNEL and FFPE and after all the years I'm happy with things. I have not noticed false positives at all when looking at TUNEL FFPE versus frozen or whole cells and even when adapting the animal model to other verifying procedures (annexinV in flow) or caspace-3 when applicable. Formalin can cause strand breaks, that is true but these are not amplifiable breaks. For TdT to work, the break must be of particular kinds. But that discussion is far beyond the realm of possibility in HistoNet. Up at UW, if you haven't done so, there is a great Cell and Molecular Biology Grad level course that is required in grad school and I'd recommend it for anyone. It is phenomenal to help in understanding molecular mechanisms. When I was down the hallway in grad school in Biological Structure, we had a PhD/MD student in adjacent lab who was working on apoptosis and macrophage scavenging in the developing limb buds (the web between fingers) for a thesis. Did TUNEL on FFPE and it was exquisite, beautiful and accepted as a (small part) of thesis. Thousands of peer-reviewed articles in prestigous journals use TUNEL on FFPE. Indeed, a review of specific articles where they are are trying to measure precisely strand breaks of DNA in UVA skin or germ-cell or other models, every one I see, the specimen is then placed into formalin or such for processing. Why would they try to get a precise measurement of DNA strand breaks in their experimental model only to throw specimens into formalin if that is going to cause ubiquitous strand breaks and flood the assay? All DNA strand breaks are not the same and the kind caused by formalin are simply not amplifiable by TdT. If they were, you could simply place a piece of normal, healthy mouse liver or some other tissue with non-apoptosing cells in formalin, process and every nuclei in liver, with broken strands from formalin would turn positive. I've, never, ever seen such a thing in a well set up TUNEL assay. And then if you do something to cause apoptosis, those nuclei are positive while others remain unaffected by formalin. Since I worked in thymus a lot,I had a control block of 4 thymii, one no treatment and the other 3 with varying time treatments of hydrocortisone to induce steroidal T-cell apoptosis (widely accepted model). The differences where easily recognizable in apoptosis and when run with other tissues, non-apoptosing cells were still clean as anything. (1) strand breaks by formalin not amplifiable in TUNEL, otherwise every nuclei formalin fixed would be pos. Just not so. (2) microtomy could not possibly cause strandbreaks that are amplifiable. Cutting is working on a micron scale. Pieces of DNA are at nanometer or Angstrom scale. If it did, again, every nuclei would be possitive that the blade sliced through, And then why would frozen TUNEL be ok if a blade is slicing through DNA in those sections. So I disagree that every one of them (nuclei in section) is detectable by TUNEL. Again as before a common piece of healthy mouse liver cut at 4 microns would show nuclei all over positive. That just doesn't happen. What I've seen and done, with very specifically controlled experiments, the vast amount of peer-reviewed literature and thinking through the processes at a molecular level, I just can't come to the conclusion that TUNEL on FFPE is a failed assay that cannot work. No assay is perfect. PCR has primer-dimer and other problems to deal with. IHC has false pos and false neg to worry about. Flow has Fc receptor problems to deal with. ISH has stringency issues to create false pos and false neg. I think there is beyond overwheming evidence that TUNEL on FFPE is an essential (if never perfect) tool in molecular science for apoptosis but as with every assay you have to be aware of limitations and problems. I just don't believe at all that amplifiable formalin strand breaks and amplifiable microtome strand beaks are any problem at all and should not be a reason to turn from TUNEL on FFPE. But again, that is just my opinion that is no more valid than others who might differ. Ray Raymond Koelling PhenoPath Labs Seattle, WA 98155 rocedure called microtomy. When a microtome bvlade passes through the nucleus of a cell it breaks a lot of DNA strands. And every one of them is detectable by TUNEL. I've heard of people getting rerasonable results with whole cells and frozen tissues, by for FFPE tissue, my current philosophy is: It is an assay that CANNOT work, even in principle. 'Course, I've been wrong about other things, I'm open to persuasion. Jerry Ricks Research Scientist University of Washington Department of Pathology
[Histonet] Sakura DRS Stainer
Is there anyone using the Sakura DRS Stainer for HE Staining?? I am presently trying to validate an HE stain using the following reagents?:? ?Hematoxylin? - Cardinal Health, 7211 Clarifier - Cardinal Health Bluing reagent - Cardinal Health Eosin Y - Cardinal Health I am decerating the slides for 5 minutes each in xylene (2 changes), 10 minutes in hematoxylin, 30 seconds in clarifier and bluing, and 1 minute in eosinobviously?there are alochol and water exchanges in between.? (This protocol works perfect on a Leica XL stainer): The hematoxylin staining is very light.? I will continue to play with the hematoxylin and clarifier times, but if someone has something that works, I would appreciate it if you could share it with me! Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eosin in Alcohol
A couple of weeks ago I posted the message below on the histonet and all of you responded that it shouldn't matter so I have finally gotten a reply from the company we send our prostate biopsies off to and below is their response. So now you know the rest of the story! We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) Their reply: The problem is that eosin belongs to a family of polycyclic aromatic flourescent compounds that in high concentrations binds to and saturates all tissue components. When immunoflourescence is performed on such tissue- as in the prostate px+ test- the diffuse background autoflourescence signal from prior treatment with these compounds can interfere with, and even totally overwhelm, the signal of the flourescent-labeled antibodies used to localize biomarkers in the tissue. _ Lauren found her dream laptop. Find the PC that’s right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Special stainers
Artisan does it for me - good stains, good backup, few if any delivery delays, application specialists 'on tap' via email direct fromDako Denmark - AND the machine does a MEAN Warthin Starry!!! Annie in Arabia 2009/7/14 kristen arvidson arvidsonkris...@yahoo.com What stainer is everyone liking the best? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Special stainers
We also use and like the Artisan. Joe Galbraith University of Iowa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, July 14, 2009 9:42 AM To: kristen arvidson; histonet Subject: RE: [Histonet] Special stainers The Artisan (DAKO) is the best. Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Tuesday, July 14, 2009 9:13 AM To: histonet Subject:[Histonet] Special stainers What stainer is everyone liking the best? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Combining Cytology and Histology
Hello, Are there any sites that are using CoPath with one number wheel for accessioning Cytology and Histology cases together? Right now, we have two separate departments with Cytology ordering under C09- and Histology ordering under S09- and we have have totally separate specimen classes. We are wondering if it's possible to use a single number wheel, using a combined number such as P09- (for Pathology), but still have each department order their own tests. This way, for example, when Cytology enters a FNA specimen in the morning and we (Histology) get a biopsy on the same patient a few hours later, when we enter the patient we are notified by the CoPath system that specimens were already assigned to that patient and we can use the same accession number. When the case is complete, everything is in one tray and can be signed out all together. One of our docs wants to have everything from Histology and Cytology on one report for the patient. Is this possible? Can Cytology still result the case if we have one accession number for everything? Any help or ideas on this issue would be greatly appreciated. Thanks much! Karen Bauer Karen L. Bauer HT(ASCP), BS Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.ka...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: survivin antibody
The survivin antibody that I have used and gotten good results with was Cell Signaling #2803 at concentrations between 1:30-1:50, using an autostainer with pretty standard protocols. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatan...@tgen.org www.tgen.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin for Processing and embedding
Hi All, I am thinking of switching the paraffin we are using for our processor and for embedding. We are using the Fisherbrand Paraplast Plus which contains DMSO for both the processor and for embedding. I used to use Suripath's paraplast and loved it. I would like to know what everyone in histoland is using. Thanks, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 E-Mail: aallison-ta...@apmglab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Film for electron microscopy
We have recently used MACO ORT25c film in unperforated 35mm format in transmission electron microscopy. It is touted as a replacement for Kodak Technical Pan Film. After dessicating the film, it demonstrates a separation of the emulsion from the film base primarily at the cut ends of film strips but also randomly along the length. This occurs before and during processing. We are interested to learn of any reports on this problem and any insight into causes and solutions. This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Human VIMENTIN and SMA IHC
Dear Histonetters! I am wondering if anyone can possibly advise good antibodies for HUMAN anti alpha SMA and Vimentin. I'm working with xenografts, human tumors with mouse stroma and in the past had a lot of cross reactivity and background issues. Does anyone know good antibodies or a clone, or has a good protocol for either??? All would be greatly appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What percent of HTL's do not have a BS degree?
Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Benchmark XT for sale
Hello HistoNetters We are selling our Ventana Benchmark XT IHC stainer. If anyone is interested, please see the ad on our web site. http://www.sarapath.com/equipment4sale/ I would be happy to answer any questions, please feel free to email or contact me directly. Thank You Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology (941) 362-8963 (941) 362-8964 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What percent of HTL's do not have a BS degree?
Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What percent of HTL's do not have a BS degree?
Tom, I missed being grandfathered in by a matter of months and am about 10 years away from retirement. I personally know of several HTLs that don't have Bachelor degrees that are around my same age. So in my part of the Universe, non-degreed HTLs are slightly more prevalent than a needle in a haystack but not much. Been at it going on 35 years, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 2:16 PM To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] What percent of HTL's do not have a BS degree?
HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that a monkey can do our job. I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce jwe...@sjha.org wrote: Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid
RE: [Histonet] What percent of HTL's do not have a BS degree?
MB, I think your prediction, sadly, is true and that it is already happening. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent: Tuesday, July 14, 2009 3:50 PM To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that a monkey can do our job. I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce jwe...@sjha.org wrote: Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message-
RE: [Histonet] What percent of HTL's do not have a BS degree?
I am with you Joyce, still going strong for 47 years and with no plans to retire, actually working my day job and a part time. I may not make it to 100 like you, but they will roll me out feet first. There are more of us around than people know. Scary huh? Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, July 14, 2009 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the
RE: [Histonet] Eosin in Alcohol
polycyclic aromatic flourescent compounds that in high concentrations I wouldn't think the 3 mls of eosin dropped in the last 95% alcohol could be considered high concentration but that's what keeps Histonet entertaining; I learn something new every day. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Tuesday, July 14, 2009 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin in Alcohol A couple of weeks ago I posted the message below on the histonet and all of you responded that it shouldn't matter so I have finally gotten a reply from the company we send our prostate biopsies off to and below is their response. So now you know the rest of the story! We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) Their reply: The problem is that eosin belongs to a family of polycyclic aromatic flourescent compounds that in high concentrations binds to and saturates all tissue components. When immunoflourescence is performed on such tissue- as in the prostate px+ test- the diffuse background autoflourescence signal from prior treatment with these compounds can interfere with, and even totally overwhelm, the signal of the flourescent-labeled antibodies used to localize biomarkers in the tissue. _ Lauren found her dream laptop. Find the PC that's right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290__ _ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] What percent of HTL's do not have a BS degree?
I wouldn't say that histology is a dead end job, I mean, I jumped to a PA and I know that I couldn't have passed that exam without my histology experience. The idea of adding an HTL category was initially a good one. Trying to bring us up to the MT and CT levels, but honestly, I think it fell short. Even after I received my BS, people asked me if I was going for my HTL. I told them no because I saw no benefit from it. At that time, I felt that if I couldn't get hired as a supervisor with a BS and 20 years experience, with 12 as a supervisor, I wasn't going to get the job any way. I'm not degrading HTLs by a long shot. I'm just saying for me, it wasn't worth it. After I retired from the Air Force, I was directly hired as a supervisor without the HTL. Just my three cents (inflation you know) JTT - Original Message - From: pathr...@comcast.net To: Michael Bradley jaustin1...@gmail.com Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:18 PM Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. Ron Martin, BS, HTL (ASCP) - Original Message - From: Michael Bradley jaustin1...@gmail.com To: Joyce Weems jwe...@sjha.org Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that a monkey can do our job. I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce jwe...@sjha.org wrote: Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in
[Histonet] RE: Combining Cytology and Histology
We are NOT using Copath however... I think the only time you have to separate Cytology from Histology is when you are dealing with GYNs. Otherwise, you can and should use the S09 (or what ever you give Pathology Surgicals). Why would anyone want to have two separate reports on one specimen? Your pathologist is right on! MK -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, July 14, 2009 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Combining Cytology and Histology Hello, Are there any sites that are using CoPath with one number wheel for accessioning Cytology and Histology cases together? Right now, we have two separate departments with Cytology ordering under C09- and Histology ordering under S09- and we have have totally separate specimen classes. We are wondering if it's possible to use a single number wheel, using a combined number such as P09- (for Pathology), but still have each department order their own tests. This way, for example, when Cytology enters a FNA specimen in the morning and we (Histology) get a biopsy on the same patient a few hours later, when we enter the patient we are notified by the CoPath system that specimens were already assigned to that patient and we can use the same accession number. When the case is complete, everything is in one tray and can be signed out all together. One of our docs wants to have everything from Histology and Cytology on one report for the patient. Is this possible? Can Cytology still result the case if we have one accession number for everything? Any help or ideas on this issue would be greatly appreciated. Thanks much! Karen Bauer Karen L. Bauer HT(ASCP), BS Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.ka...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What percent of HTL's do not have a BS degree?
Too bad someone doesn't do a 'survey' on this site where the question can be answered with real data... MK -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, July 14, 2009 2:37 PM To: pathr...@comcast.net; Michael Bradley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? I wouldn't say that histology is a dead end job, I mean, I jumped to a PA and I know that I couldn't have passed that exam without my histology experience. The idea of adding an HTL category was initially a good one. Trying to bring us up to the MT and CT levels, but honestly, I think it fell short. Even after I received my BS, people asked me if I was going for my HTL. I told them no because I saw no benefit from it. At that time, I felt that if I couldn't get hired as a supervisor with a BS and 20 years experience, with 12 as a supervisor, I wasn't going to get the job any way. I'm not degrading HTLs by a long shot. I'm just saying for me, it wasn't worth it. After I retired from the Air Force, I was directly hired as a supervisor without the HTL. Just my three cents (inflation you know) JTT - Original Message - From: pathr...@comcast.net To: Michael Bradley jaustin1...@gmail.com Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:18 PM Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. Ron Martin, BS, HTL (ASCP) - Original Message - From: Michael Bradley jaustin1...@gmail.com To: Joyce Weems jwe...@sjha.org Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that a monkey can do our job. I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce jwe...@sjha.org wrote: Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world.
[Histonet] HTL/BS
Thought I would throw my 2 cents out there, too. I received my HTL back in the early 80's (hate to think how old that makes me!), I already had a BS when I went to histotech school. I have found that it has made a difference in my career to have the HTL. When I have worked in hospitals, I was on the same pay scale as the med techs, the HT's were not. When I received my QIHC, some kind med techs lobbied for me to get the same specialty certification bonus that the med techs received. I feel fortunate to have mostly been treated by physicians and co-workers with respect, and do feel that my work is a profession ( I enjoy it). I am thankful to have the wonderful experiences, and I go home have a glass of wine on the bad days! Patti Loykasek This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What percent of HTL's do not have a BS degree?
I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen as a career. The hospitals as well as the doctors are also to blame. I am very proud to have a B.S. and A.S.S. degree and an HTL certification. I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. It makes me sad to hear people say that this is just a job not a career. I do not believe that anyone should be allowed to just come off the street and do our job. It up to us as a community to demand that institutions require certification and recognize our educations. I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. I didn't waste all that money on just a job this is my career and I am very proud of the work I do. Nikki -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Tuesday, July 14, 2009 4:18 PM To: Michael Bradley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. Ron Martin, BS, HTL (ASCP) - Original Message - From: Michael Bradley jaustin1...@gmail.com To: Joyce Weems jwe...@sjha.org Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that a monkey can do our job. I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce jwe...@sjha.org wrote: Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences
Re: [Histonet] What percent of HTL's do not have a BS degree?
Just my opinion Nikki. Sorry you don't agree but at least I respect your opinion. - Original Message - From: Nikki Wahlberg nikki.wahlb...@bsci.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 7:02:48 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen as a career. The hospitals as well as the doctors are also to blame. I am very proud to have a B.S. and A.S.S. degree and an HTL certification. I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. It makes me sad to hear people say that this is just a job not a career. I do not believe that anyone should be allowed to just come off the street and do our job. It up to us as a community to demand that institutions require certification and recognize our educations. I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. I didn't waste all that money on just a job this is my career and I am very proud of the work I do. Nikki -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Tuesday, July 14, 2009 4:18 PM To: Michael Bradley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. Ron Martin, BS, HTL (ASCP) - Original Message - From: Michael Bradley jaustin1...@gmail.com To: Joyce Weems jwe...@sjha.org Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that a monkey can do our job. I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce jwe...@sjha.org wrote: Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were
Re: [Histonet] What percent of HTL's do not have a BS degree?
To Ron Martinhistology IS BOTH A PROFESSION AND A CAREER! You need a new job! Colleen Forster HT(ASCP)QIHC Wahlberg, Nikki wrote: I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen as a career. The hospitals as well as the doctors are also to blame. I am very proud to have a B.S. and A.S.S. degree and an HTL certification. I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. It makes me sad to hear people say that this is just a job not a career. I do not believe that anyone should be allowed to just come off the street and do our job. It up to us as a community to demand that institutions require certification and recognize our educations. I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. I didn't waste all that money on just a job this is my career and I am very proud of the work I do. Nikki -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Tuesday, July 14, 2009 4:18 PM To: Michael Bradley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. Ron Martin, BS, HTL (ASCP) - Original Message - From: Michael Bradley jaustin1...@gmail.com To: Joyce Weems jwe...@sjha.org Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that a monkey can do our job. I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce jwe...@sjha.org wrote: Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there
[Histonet] formalin substitutes - tissue structure
Dear Histonetters, I have a question about alternatives to formalin fixation and fine changes in tissue structure. We have been obtaining formalin fixed human skin and fat samples from several companies. We use stereological methods to make tissue measurements such as dermal thickness and adipose cell size from sections stained with a variety of basic stains. However, there is now another company that we would like to do obtain more tissue from but they can only provide tissue fixed with a formalin alternative such as FineFix or Prefer. Measurement data collected from formalin and formalin alternative fixed tissue would be used together if we obtained tissue from this other company. From the Histonet archives I see that sometimes the use of formalin alternatives can affect immuno staining, but does anyone know how it would affect fine structure of tissue. My thoughts were that there may be a slight difference in 'shrinkage' that occurs given the main ingredient is ethanol on some of the alternatives, so fat fixed in one of these alternatives would give an erroneously smaller adipose cell size. Any insight would be greatly appreciated. Thank you Yak-Nam Wang University of Washington ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] What percent of HTL's do not have a BS degree?
Colleen, One of my points of frustration is that we have never been on the same level as MT's and MLT's. I feel that part of this is because of our level of education and requirements. After seeing little changes over the years it is frustrating. I'm sure I'm not the only one who feels this way. Yes, I would like to find another area to utilize my education and background. My apologies if I offended anyone as that was not my intention. All opinions should be respected. Ron - Original Message - From: Colleen Forster cfors...@umn.edu To: Nikki Wahlberg nikki.wahlb...@bsci.com Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 7:32:06 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? To Ron Martinhistology IS BOTH A PROFESSION AND A CAREER! You need a new job! Colleen Forster HT(ASCP)QIHC Wahlberg, Nikki wrote: I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen as a career. The hospitals as well as the doctors are also to blame. I am very proud to have a B.S. and A.S.S. degree and an HTL certification. I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. It makes me sad to hear people say that this is just a job not a career. I do not believe that anyone should be allowed to just come off the street and do our job. It up to us as a community to demand that institutions require certification and recognize our educations. I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. I didn't waste all that money on just a job this is my career and I am very proud of the work I do. Nikki -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Tuesday, July 14, 2009 4:18 PM To: Michael Bradley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. Ron Martin, BS, HTL (ASCP) - Original Message - From: Michael Bradley jaustin1...@gmail.com To: Joyce Weems jwe...@sjha.org Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that a monkey can do our job. I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce jwe...@sjha.org wrote: Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From:
Re: [Histonet] What percent of HTL's do not have a BS degree?
Hey everyone, I'm actually a early 20s histotech without a certification and run my own routine histo lab. *waits for young whiper snapper remark* But I am in the process of completing my education. In all seriousness though I have to agree many out there are looking for cheap labor - so I take it upon myself to get my cert through a local program and look for jobs that are more management based and less bench work (or high paying contract work) Honestly the only true advancement I see in my career is management or consulting, but take that with a grain of salt - and throw in some fiber while your at it :P. I've only been in the field for a year. Sent from my Verizon Wireless BlackBerry -Original Message- From: pathr...@comcast.net Date: Tue, 14 Jul 2009 23:40:15 To: Colleen Forstercfors...@umn.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Colleen, One of my points of frustration is that we have never been on the same level as MT's and MLT's. I feel that part of this is because of our level of education and requirements. After seeing little changes over the years it is frustrating. I'm sure I'm not the only one who feels this way. Yes, I would like to find another area to utilize my education and background. My apologies if I offended anyone as that was not my intention. All opinions should be respected. Ron - Original Message - From: Colleen Forster cfors...@umn.edu To: Nikki Wahlberg nikki.wahlb...@bsci.com Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 7:32:06 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? To Ron Martinhistology IS BOTH A PROFESSION AND A CAREER! You need a new job! Colleen Forster HT(ASCP)QIHC Wahlberg, Nikki wrote: I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen as a career. The hospitals as well as the doctors are also to blame. I am very proud to have a B.S. and A.S.S. degree and an HTL certification. I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. It makes me sad to hear people say that this is just a job not a career. I do not believe that anyone should be allowed to just come off the street and do our job. It up to us as a community to demand that institutions require certification and recognize our educations. I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. I didn't waste all that money on just a job this is my career and I am very proud of the work I do. Nikki -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Tuesday, July 14, 2009 4:18 PM To: Michael Bradley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. Ron Martin, BS, HTL (ASCP) - Original Message - From: Michael Bradley jaustin1...@gmail.com To: Joyce Weems jwe...@sjha.org Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more
[Histonet] RE: human vimentin IHC
Igor. A few years back I used Dako V9 mouse anti human vimentin to label human grafts in a mouse background. Tested it first on several mouse tissues and got no reactivity, compared to very strong reactivity in a variety of cell types in human tissue, and so am sure that it is human specific cf mouse. I used the Dako ARK to get around the mouse-on-mouse background issues and was happy with the staining obtained (although not quite as sensitive perhaps as a standard, LSAB method). I am actually about to try this again myself very soon. I used citrate AR and primary at 1:800 for my staining. Cheers, Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.pal...@svhm.org.au -- Message: 4 Date: Tue, 14 Jul 2009 15:03:47 -0400 From: Igor Deyneko igor.deyn...@gmail.com Subject: [Histonet] Human VIMENTIN and SMA IHC To: Histonet@lists.utsouthwestern.edu Message-ID: 35e16a770907141203h14ccc18bt2e3123d11c478...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Dear Histonetters! I am wondering if anyone can possibly advise good antibodies for HUMAN anti alpha SMA and Vimentin. I'm working with xenografts, human tumors with mouse stroma and in the past had a lot of cross reactivity and background issues. Does anyone know good antibodies or a clone, or has a good protocol for either??? All would be greatly appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HTL
Michael, Ditto, very well stated. I too believe that our industry is under appreciated. Many new grads of today find a two year degree demeaning and wouldn't consider HT because of it. I don't understand how some professions like pharmacy physical therapy gain respect and grow to create 5 yr, 6yr 7yr programs. They are very well respected by the MDs and Hospital administration and have nice salaries to show for it. Why hasn't our field flourished? Jan, BS, HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
