[Histonet] Histology Job Opening
To All We have a Histology Job Opening Day Shift 730a to 4 pm. Please call or send resume. Thanks Lea S. Alminde Anatomic Pathology Supervisor Jeanes Hospital 215-728-2034 email almin...@tuhs.temple.edu This electronic message is intended to be for the use of the named recipient, and may contain information that is confidential or privileged. This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Pennsylvania Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete and destroy all copies of this message. Direct Questions to the Corporate Compliance and Privacy Officer at (215) 707-5605. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histology for kids
Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formula 83
Does anyone have any experience with Formula 83. We are evaluation it now and I would like to know how frequently it needs to be changed on the processor. The label says use just like xylene...BUT is there anyone out in HistoLand that uses it? I would like to know your experience in processing. Right now we are using it on a VIP. Thanks!!! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formula 83
Phyllis, I have used Formula 83 for several years and have no problem with it. I do use it just like xylene. As for how frequently you need to change it on the processor really depends on your volume of processing. I didn't have to change the frequency when I switched from xylene to Formula 83. I also recycle my Formula 83. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Wednesday, July 22, 2009 10:04 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83 Does anyone have any experience with Formula 83. We are evaluation it now and I would like to know how frequently it needs to be changed on the processor. The label says use just like xylene...BUT is there anyone out in HistoLand that uses it? I would like to know your experience in processing. Right now we are using it on a VIP. Thanks!!! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histology for kids
Try saffron, in reality it is an acceptable stain for regular grown-up histology as well. René J. --- On Wed, 7/22/09, Kim Merriam kmerriam2...@yahoo.com wrote: From: Kim Merriam kmerriam2...@yahoo.com Subject: [Histonet] histology for kids To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, July 22, 2009, 9:57 AM Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech II position open.
Hello all. I would like to inform the histotech community of an opening for a Histotech II position. We are looking for someone with experience who is willing to learn. Renal, Muscle, and IHC experience is a definite plus, but we are willing to teach if the candidate is motivated. Please apply at www.uthouston.eduhttp://www.uthouston.edu and follow the careers link to the allied health category. Thanks! Richard A. Breckenridge, HT(ASCP) Technical Director/ Chief Histology Technician University of Texas- Houston Medical School Histology/ IHC Laboratory Office 713-500-6792 IHC Lab 713-500-5096 Histology Lab 713-500-5363 FAX 713-500-0733 Email - richard.breckenri...@uth.tmc.edumailto:richard.breckenri...@uth.tmc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histology for kids
Maybe you could use a sponge (representing tissue) soaked in water and demonstrate cutting (ragged) vs. a sponge soaked in wax and cooled (precise cutting) explaining the water is taken out of the cells and replaced with wax. Rene J Buesa rjbu...@yahoo.com 07/22/2009 07:15 Try saffron, in reality it is an acceptable stain for regular grown-up histology as well. René J. --- On Wed, 7/22/09, Kim Merriam kmerriam2...@yahoo.com wrote: From: Kim Merriam kmerriam2...@yahoo.com Subject: [Histonet] histology for kids To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, July 22, 2009, 9:57 AM Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histology for kids
red food colouring, green and blue too as well as beetroot and grape juice and saffron could be tried in advance on the slides - just to see how colours can be combined you will only know by testing in advance - or you may end up with a brown sludge covering the whole section look at hairs and fleas and tiny bugs under the 'scope - they always intrigue kids skin scales on a slide stained with 3 diff kwik solutions or a PAP stain would work too just my 5cents worth Anne 2009/7/22 Kathleen Boozer booze...@ah.org Maybe you could use a sponge (representing tissue) soaked in water and demonstrate cutting (ragged) vs. a sponge soaked in wax and cooled (precise cutting) explaining the water is taken out of the cells and replaced with wax. Rene J Buesa rjbu...@yahoo.com 07/22/2009 07:15 Try saffron, in reality it is an acceptable stain for regular grown-up histology as well. René J. --- On Wed, 7/22/09, Kim Merriam kmerriam2...@yahoo.com wrote: From: Kim Merriam kmerriam2...@yahoo.com Subject: [Histonet] histology for kids To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, July 22, 2009, 9:57 AM Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide dryers
Hi All. We are re-working some of our processes (fun!). We are trying to get more LEAN and shave some time off of our processes here there. One thing I am looking at is the small forced air slide dryers. I have seen some good comments on the mopec roto-dry. Has anyone tried the dryer from EMS? What are people using and liking? We will be doing IHC on these sections. What type of drying time are you using? I really appreciate the info. We are having a series of quick meetings, and I am having to gather information quickly. Thanks for the help. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Formula 83
We discussed formula 83 earlier this year. http://lists.utsouthwestern.edu/pipermail/histonet/2009-January/041976.html Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Supervisor needed in Washington State
Hello Histonetters! I have a new position I want to tell you about. I am currently working with a hospital located in Washington State who is in need of a histology supervisor. ASCP HT, Hands -on histology and supervisory experience is required. My client offers a great salary and benefits and relocation assistance. If you or anyone you know might be interested please contact me at 866-607-3542 or rel...@earthlink.net. Thanks-Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: rel...@earthlink.net mailto:rel...@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] histology for kids
Try this website for ideas... http://www.mnmicroscopy.org/ProjectMicro/Welcome.html Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, July 22, 2009 6:58 AM To: Histonet Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Start Up Lab
And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology for kids
I did tonsils one time and since most of them have had theirs removed, they were very interested. We also have autopsy tissue, brains, lung, liver, heart, in jars of formalin which are always a hit for them to see but not touch! Good Luck, it will be so much fun watching their faces. Jennifer Johnson, HTL (ASCP) _ Windows Live™ Hotmail®: Search, add, and share the web’s latest sports videos. Check it out. http://www.windowslive.com/Online/Hotmail/Campaign/QuickAdd?ocid=TXT_TAGLM_WL_QA_HM_sports_videos_072009cat=sports___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] histology for kids
Kim, Contact Mary McCann who is ProjectMICRO's coordinator for NESM (New England Society for Microscopy). NESM took on ProjectMICRO as a pet project a number of years back. We put together 3 kits of materials (microscopes and consumables). Two of the kits are in constant use in Vermont and Maine. Mary is in charge of the 3rd kit around this area. She also comes in and does festivals which consist of different exploratory stations. Go to NESM's website: http://nesm.cims.harvard.edu/ and click on ProjectMICRO. Mary's contact info is there. I have done several of these festivals and also worked at the Cambridge Science Day. The kids love these exercises! Good luck and have fun! Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, July 22, 2009 9:58 AM To: Histonet Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] histology for kids
A number of dyes used in histology are also approved for use in foods. These include: Brilliant Blue (FDC Blue #1) Fast Green FCF (FDC Green #3) Erythrosin (FDC Red #3) Tartrazine (FDC Yellow #5) Carmine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Citadel 2000 Tissue Processor
Hello, will everyone who's used/using the Citadel 2000 Tissue Processor please share with me your opinions, pros/cons and etc ASAP? Thanks! Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] histology for kids
If you ripen it with air or sodium iodate, alum hematoxylin is quite safe. FDC green #3 is food grade fast green FCF, an excellent stain for collagen. FDC yellow #5 is tartrazine, a plasma stain. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, July 22, 2009 9:58 AM To: Histonet Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histology for kids
One more thought, NSH has a small paper pamphlet out call Histology (hiss TOL-o-je) which has pictures, puzzles, anatomy charts (simple) to help teach kids. Written by Judy Stasko, CLT and Jan Gardiner, BAAS, HT(ASCP). Kathleen Boozer booze...@ah.org 07/22/2009 07:46 Maybe you could use a sponge (representing tissue) soaked in water and demonstrate cutting (ragged) vs. a sponge soaked in wax and cooled (precise cutting) explaining the water is taken out of the cells and replaced with wax. Rene J Buesa rjbu...@yahoo.com 07/22/2009 07:15 Try saffron, in reality it is an acceptable stain for regular grown-up histology as well. René J. --- On Wed, 7/22/09, Kim Merriam kmerriam2...@yahoo.com wrote: From: Kim Merriam kmerriam2...@yahoo.com Subject: [Histonet] histology for kids To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, July 22, 2009, 9:57 AM Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] More quality stuff
Hi- As most of you have gathered I am working on a large quality project. I have made a lot of progress. Currently, my focus is error prevention. I have read up on tools such as six sigma and FMEA. My question is how do you guys prevent errors? I have learned that you cannot always count on the diligence of the employee because even the best will screw up sooner or later, so my focus is on process improvement. How are you all approaching your process improvements? Thank you all for your input. You've all been a big help!! -Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] More quality stuff
We have had tremendous success with setting up checkpoints at each step in tissue processing (cradle to grave). Each checkpoint has certain responsibilities to identify, double-check, and assure accuracy. I keep track of discrepancy errors at each checkpoint and determine if personnel need to be re-trained, held accountable, or possibly add or revise checkpoints. It takes a lot of time to do this. What I have found though, is that we prevent most errors by the checkpoint after it initially happens and long before it goes out of the department. Kathy kristen arvidson arvidsonkris...@yahoo.com 07/22/2009 09:58 Hi- As most of you have gathered I am working on a large quality project. I have made a lot of progress. Currently, my focus is error prevention. I have read up on tools such as six sigma and FMEA. My question is how do you guys prevent errors? I have learned that you cannot always count on the diligence of the employee because even the best will screw up sooner or later, so my focus is on process improvement. How are you all approaching your process improvements? Thank you all for your input. You've all been a big help!! -Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] More quality stuff
Hi Kathy, Do you have a sample form you would be willing to share? thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 Kathleen Boozer booze...@ah.org 07/22/09 1:26 PM We have had tremendous success with setting up checkpoints at each step in tissue processing (cradle to grave). Each checkpoint has certain responsibilities to identify, double-check, and assure accuracy. I keep track of discrepancy errors at each checkpoint and determine if personnel need to be re-trained, held accountable, or possibly add or revise checkpoints. It takes a lot of time to do this. What I have found though, is that we prevent most errors by the checkpoint after it initially happens and long before it goes out of the department. Kathy kristen arvidson arvidsonkris...@yahoo.com 07/22/2009 09:58 Hi- As most of you have gathered I am working on a large quality project. I have made a lot of progress. Currently, my focus is error prevention. I have read up on tools such as six sigma and FMEA. My question is how do you guys prevent errors? I have learned that you cannot always count on the diligence of the employee because even the best will screw up sooner or later, so my focus is on process improvement. How are you all approaching your process improvements? Thank you all for your input. You've all been a big help!! -Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histology for kids
Thanks to everyone that emailed me, I received so many ideas! I will let you all know what I end up donig. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Kim Merriam kmerriam2...@yahoo.com To: Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, July 22, 2009 9:57:53 AM Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Start Up Lab
I would add an eye wash station and a chemical spill kit to the list. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Chromium Trioxide
Deanne Knutson, Anatomic Pathology Supervisor, St. Alexius Medical Center, Bismarck, North Dakota asks: We use Chromium Trioxide in our GMS stain, and the cost has escalated tremendously. Does anyone use a substitute for this chemical? I am curious what others are using for their GMS stain. We still do our stains manually at the present time. There is no completely satisfactory substitute for chromium trioxide (chromic acid) for the oxidation step for the GMS stain for fungi, particularly if you're trying to stain Histoplasma. Many kits substitute periodic acid, usually in inadequate amounts. Freida Carson published a careful study of this problem several years ago and concluded that periodic acid could be substituted, with sufficient time and temperature. (I think I can find this reference, but it's probably already in our archives.) Last time I looked (2006) the Ventana method still used chromium trioxide. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Chromium Trioxide
Bob is correct that there is no satisfactory substitute for chromic acid in the GMS procedure. We found that it takes 1 hour at 56-60 degrees C in 1% periodic acid to equal the usual oxidation with chromic acid, and even then you will probable get more silver staining of the connective tissue than with chromic acid. The paper was published in the J Histotechnol, 1999; Vol 22:119. If you rinse sections very well with water before the chromic acid, the chromic acid can be used for over and over. It is alcohol remaining on the slides that causes the chromic acid to turn brown and become unusuable. Hope this helps. Freida Carson --- On Wed, 7/22/09, Robert Richmond rsrichm...@gmail.com wrote: From: Robert Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Chromium Trioxide To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 22, 2009, 2:02 PM Deanne Knutson, Anatomic Pathology Supervisor, St. Alexius Medical Center, Bismarck, North Dakota asks: We use Chromium Trioxide in our GMS stain, and the cost has escalated tremendously. Does anyone use a substitute for this chemical? I am curious what others are using for their GMS stain. We still do our stains manually at the present time. There is no completely satisfactory substitute for chromium trioxide (chromic acid) for the oxidation step for the GMS stain for fungi, particularly if you're trying to stain Histoplasma. Many kits substitute periodic acid, usually in inadequate amounts. Freida Carson published a careful study of this problem several years ago and concluded that periodic acid could be substituted, with sufficient time and temperature. (I think I can find this reference, but it's probably already in our archives.) Last time I looked (2006) the Ventana method still used chromium trioxide. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
(lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Citadel Tissue Processor
Hi Atoska, We do only research and have low volume. We purchased a second hand one and used it for about a year. It works and does the job. It is much better than having to process manually but: The alcohols evaporate like crazy! You loose a lot. It must be in a hood. There is no heating nor vacuum. I was under the impression that there is vacuum while reading the brochure, but when the Citadel arrived, I found that it is a separate free standing unit that can be connected only with the paraffin tanks. We didn't have the room for it so we never used it and sent it back. There is no purge/clean cycle. You have to either purchase 2 extra buckets or find some other containers for the Xylene and the 100% which you will be using to manually wash the basket after the cassettes are out of it. They should also be in the hood. In a year, one of the paraffin buckets burned. The service guy told me that a repair was not worth it. A new paraffin bath is about $1200.00. Something that was good is that you only need about 1/2 gallon per station. When we had only few cassettes, we didn't even fill completely the buckets. Infiltration was fine. Hope this helps Michelle USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Start Up Lab
Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 100 micron sections
Hi, I am being asked to section paraffin embedded fat at 100 microns. I haven't sectioned anything in paraffin thicker than 20 microns. What are the tricks to get it to unroll in the water bath? -- */-- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER./* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chromium trioxide
You can use periodic acid to oxidize your tissues as you would for the PAMS stain. However, this is not the optimal solution when staining fungi. 5% chromium trioxide used for the GMS can be reused. Since you are doing the stains manually, as many of us do, this works particularly well. The fresh chromium trioxide is a clear orange color. It can be reused until the color turns more brown in color. This indicates that the oxidizing potential has been depleted. You can also use 3% chromium trioxide instead of the 5% and heat the solution to 55 degrees and oxidize for 30 minutes. Finally you can use 2% chromic acid in the microwave oven at 70% power for 45 seconds (solution should be about 55 degrees). By using milder solutions or reusing your solution you will save money and time. Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
