RE: [Histonet] FNA SLIDES
Adequacy is carried out by the Biomedical Scientist usually in the UK. I make a couple of air dried per passage and stain Diff Quick; you can then give an indication of adequacy within 5 min or so. Usually if the FNA is performed properly you don't get much material;; for example breast and LN's are usually sparse and you get maybe a couple of slides (we don't check these for adequacy as the Site is easily re-needled). Pancreas, Thyroid and Lung are another matter. Usually CT orientated FNAC of the lung tend not to be too cellular but sometimes they are; I used to stain a couple for adequacy and make as many slides as possible. The problem is if you make loads of slides and malignant cells are not apparent in those you stain then you have to look at all the others; tough. If there are malignant cells abundant then you ought to look at all the slides just in case they hold diagnostic information. In the end we did 6 slides and washed the remainder into a pot with saline and did Cytospins if appropriate. Pancreatic FNACs taken under a ultra sound flexible scope can also be very cellular (blood) and the above statement holds. Thyroid's can be very bloody, very bloody; trick is not to pull on the plunger but let it seep into the syringe. Too much blood and what little you have gets well diluted!! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: 31 August 2009 19:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA SLIDES A question has arisen for us- How many slides do you (should you) make per pass for pathologist for adequacy and/or diagnosis? What about CT guided biopsies of liver, lung, masses etc. Thanks in advance for your input. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Endogenous biotin blocking
I recently had a discussion with one of my coworkers about the need/requirement for blocking of endoegnous biotin whenever an avidin-biotin detection system is used, and I was hoping that the IHC experts on the histonet might be able to provide us with some feeback. Its been my understanding that blocking is only necessary when one is certain that background staining is caused by endogenous biotin, but maybe I'm off-base here. I look forward to eveyone's input. Sally ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Endogenous biotin blocking
We use biotin blocking only with certain tissues, i.e. liver, kidney, GI, or diseases, i.e. oncocytomas. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sally Price Sent: Tuesday, September 01, 2009 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Endogenous biotin blocking I recently had a discussion with one of my coworkers about the need/requirement for blocking of endoegnous biotin whenever an avidin-biotin detection system is used, and I was hoping that the IHC experts on the histonet might be able to provide us with some feeback. Its been my understanding that blocking is only necessary when one is certain that background staining is caused by endogenous biotin, but maybe I'm off-base here. I look forward to eveyone's input. Sally ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PIN 4
I went to archives to look for this but I didn't get a definite answer to my specific question. I am using Biocare's Prostate Cocktail - 2X (CK5 + CK14 + p63) and Biocare's P504S-2X on the Ventana platform using UltraView DAB and Ultra View RED kits. To code this procedure, is it 88342 X 3 or 88342 X 4? I would like some rationale as well. Thanks! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re: [Histonet] Re: uneven alternating sections on cryostat
that's funny~~ just keep everything tight and unmovable. also, we use anti-roll guide to keep the sections flat so, sometimes there is a bit OCT attached to the blade/glass/anti-roll guide or surrounding the tissue. clean it. have you tried to cut at a higher temperature? the tissue could be too hard ! this always happen if you are using the frozen microtome, but applies to cryostat as well. 2009-09-01 TF 发件人： Emily Sours 发送时间： 2009-08-28 22:58:24 收件人： Johnson, Teri 抄送： histonet 主题： Re: [Histonet] Re: uneven alternating sections on cryostat You've gotten great advice so far, but if it doesn't help--our problem was that the specimen head was loose--even when it was locked, it would move very slightly. This has to be fixed by servicing it, unfortunately. It was caused by our MD/PhD student trying to adjust the specimen head when it was locked, hence loosening the screws. It happened even though I told the guy to quit doing it, which drove me mad. How some people get this far is beyond me. Emily One of the defining characteristics of modern surgery was that patients ought to survive it. --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] PIN 4
Its three... Two different chromagens, 1 nuclear and two cytoplasmic abs, which cannot be differentiated. You can bill for what can be separately identified. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Tuesday, September 01, 2009 09:56 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PIN 4 I went to archives to look for this but I didn't get a definite answer to my specific question. I am using Biocare's Prostate Cocktail - 2X (CK5 + CK14 + p63) and Biocare's P504S-2X on the Ventana platform using UltraView DAB and Ultra View RED kits. To code this procedure, is it 88342 X 3 or 88342 X 4? I would like some rationale as well. Thanks! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE preparation
Dear All, We are preparing harris heamtoxylin for HE in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _ News, sports, entertainment and fine living…learn the ropes on MSN India http://in.msn.com___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vitamin D Receptor Staining
I have been trying perform immunohistochemisty on formalin-fixed paraffin-embedded skin samples with a vitamin D receptor antibody with no luck. I'm using the Abnova antibody VDR monoclonal, clone 2F4 (catalog #H7421-M02). Has anyone used this antibody, even on non-skin samples? If so, what was your protocol? Or, if you have used a different mouse VDR antibody, could you let me know how it worked? Thanks! Joanna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re:Endogenous biotin blocking
Your understanding is correct, imho. I add a no primary control on my stABCpx-DAB run whenever I am testing new tissues and assess any positivity that could be due to endogenous biotin. You will quickly build up a knowledge of those tissues that automatically require biotin - blocking. ( eg liver, kidney) If you are a clinical/registered Lab, you wil of course be required to buy a commercial kit. If you are in a research lab and find that you will need to apply biotin blocking regularly, you can make up your own avidin/biotin solutions if costs are a major factor. I will be interested in other suggestions/approaches. carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Marginated Staining on IHC
Hi histonet, I am back with a problem I've been having with my IHC slides. We had a PM recently and had thought that would solve this problem, but as I do more and more slides, the problem remains. I am seeing marginated staining at the edge of the tissue on the controls as well as the patient tissue. This occurs at the top, middle, and bottom of the slides. This occurs with any antibody. This does not occur with every run, but the more slides I have on the stainer, the more frequently it occurs. I have contacted Thermo, who now takes care of our old R.A.S. Microm HMS 710i, but I have further faith that the histonet can help me. I've seen similar posts in the archives, but did not see any responses. Any suggestions are appreciated, Katelin Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE preparation
The hematoxylin has to be ripen, either naturally (it takes long time) or with an oxidizer. René J. --- On Tue, 9/1/09, Aazath Raj aaz...@hotmail.com wrote: From: Aazath Raj aaz...@hotmail.com Subject: [Histonet] HE preparation To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 1, 2009, 11:48 AM Dear All, We are preparing harris heamtoxylin for HE in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _ News, sports, entertainment and fine living…learn the ropes on MSN India http://in.msn.com___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Book specific for Neuropathology techniques/methods
Hi, Can anyone on the Histonet recommend a good book of Neuropathology techniques/methods for use in a Neuropathology Lab. Need more up to date reference material. Thanks Sharon Allen Neuropathology Lab HSC - Wpg sal...@hsc.mb.ca This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mast cell staining HELP!
Hi, Can anyone out there recommend the best way to stain mouse mast cells in the kidney(toluidine blue/or berberine sulfate) with a protocol that produces cconsistent results? Secondly, I have mouse kidney sections fixed in FFPE, PLP and methyl Carnoys. Does anyone know of a company that supplies antibodies which will work on any of these fixatives,for the staining of the IgE receptor, mast cell trytptase and chymase. Any advice would be appreciated! Kim O'Sullivan Monash University Melbourne Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
