[Histonet] Ink issues
Good Morning all- I am having issues getting ink to stick to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a ink stay acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to fix your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ink issues
My Docs use acetone. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues Good Morning all- I am having issues getting ink to stick to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a ink stay acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to fix your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ink issues
Good Morning, We dip the inked tissue in a container of Acetone. We use the 7-dye kit from Bradley Products, Inc. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues Good Morning all- I am having issues getting ink to stick to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a ink stay acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to fix your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ink issues
We use acetic acid - 3-5% solution (vinegar). j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 07:03 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues Good Morning all- I am having issues getting ink to stick to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a ink stay acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to fix your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE preparation
the good ole days for sure - purple ceilings, purple walls, purple people And cigarette ashes in the trash and food on the counter... Is it Friday yet?!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Wednesday, September 02, 2009 07:02 To: aaz...@hotmail.com; histonet@lists.utsouthwestern.edu; kemlo.roger...@waht.swest.nhs.uk Subject: RE: [Histonet] HE preparation Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric oxide) too soon when it was still bubbling and having a volcano of hematoxylin?!!! LOLj Was remembering tho', that every day, before using, we would top off (add...oh, about 30ml) of fresh to the filtered used batch. This really kept the quality good. Worked for 30 yrs until we started purchasing it. Gone are the morning coffee and donuts sitting next to our microtomes too!! Sigh Ya, yaI know!!! Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Robotic Prostatectomy
For the first time here, we have Urologists using the robotic method for removing prostates. The PA always receives them fresh, inks them, then puts the whole prostate in alcoholic fixative (currently Zfix from Anatech). The next day they are grossed in and processed. The prostates we have received lately are raw in the middle and unable to be grossed in, whereas before they were fine. Has anyone had similar experiences? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Does anyone have experience with the Cell Technology Apo-single stranded DNA antibody? Please let me know if you do. Thanks! Jennifer James BS, HT(ASCP)HTL, QIHC, CRS Research Assistant Experimental Pathology Laboratory Winthrop P.Rockefeller Cancer Institute, Room 429 Universtiy of Arkansas for Medical Sciences 4301 West Markham Street, #725 Little Rock, AR 72205 501-686-8265 jamesjennif...@uams.edumailto:jamesjennif...@uams.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cost to produce an HE slide
Hello Histonet.Has anyone ever figured out how much it costs to produce an HE slide? Are there any articles out there that would help to figure this out? I realize it may be different for each institution but a ball park figure would be greatly appreciated. Thanks Amylin Johnson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Robotic Prostatectomy
And your problem will not be solved unless the prostates, after being inked, are cut in half before placing them in the fixative. I do not see any problem in cutting in half a properly inked prostate to assure proper fixation and subsequent processing. René J. --- On Wed, 9/2/09, Phyllis Thaxton dch...@yahoo.com wrote: From: Phyllis Thaxton dch...@yahoo.com Subject: [Histonet] Robotic Prostatectomy To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 2, 2009, 11:26 AM For the first time here, we have Urologists using the robotic method for removing prostates. The PA always receives them fresh, inks them, then puts the whole prostate in alcoholic fixative (currently Zfix from Anatech). The next day they are grossed in and processed. The prostates we have received lately are raw in the middle and unable to be grossed in, whereas before they were fine. Has anyone had similar experiences? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Ink issues
About what to put on tissue to make the marking ink stick to it: I don't use anything. The trick is to blot the surfaces thoroughly dry with a paper towel before you try to put the ink on. Of course, there's nothing you can use that will make ink adhere well to cauterized surfaces (some breast biopsy specimens, LEEP cones, and such. The pathologist can however identify cauterized margins under the microscope.) Because of the picric acid (and because it stains your clothes) Bouin's fixative is unacceptable. So is acetone, which is a serious explosion hazard. If you must use something, use 3% acetic acid (white vinegar diluted half-strength). I've been grossing since 1965, worked on maybe sixty different pathology services. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Gloves
Janet Bonner notes: We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com.(1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box not intended to be used as a chemical barrier, but they do a great job when exposed to Histology chemicals. Not familiar with this product, but with nitrile rubber gloves brand name is important, as the quality of nitrile rubber gloves worsens. When they first came out and were made in the USA, I could make a pair last for two weeks of grossing. As manufacture moved to the latex-producing countries, nitrile rubber (if that's what they actually are) gloves became no better than latex. At present I use two pairs of gloves when I gross. There's a little-known product called chemotherapy gloves - thick blue latex. I filched a box of these and use them - they last for several days. Obviously re-using gloves offsets the high initial cost of purchase, but that's not good MBA thinking. OSHA says not to handle formaldehyde (and I suppose xylene) with latex gloves, but has not specified an alternative. It's disgraceful how little the powers that be care about the hands of pathologists and histotechnologists. You can bet that if it were nurses who had this problem, it'd get solved in a hurry. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Ink issues
I concur with the Samurai Pathologist. Long ago, we used Bouin's but have stopped that procedure and prefer to just blot dry but do also use 3% acetic acid, especially if blotting could potentially damage a delicate tissue. Good luck. Joe Galbraith University of Iowa joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, September 02, 2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ink issues About what to put on tissue to make the marking ink stick to it: I don't use anything. The trick is to blot the surfaces thoroughly dry with a paper towel before you try to put the ink on. Of course, there's nothing you can use that will make ink adhere well to cauterized surfaces (some breast biopsy specimens, LEEP cones, and such. The pathologist can however identify cauterized margins under the microscope.) Because of the picric acid (and because it stains your clothes) Bouin's fixative is unacceptable. So is acetone, which is a serious explosion hazard. If you must use something, use 3% acetic acid (white vinegar diluted half-strength). I've been grossing since 1965, worked on maybe sixty different pathology services. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Gloves
It says on the box that these aloe touch nitrile gloves are Latex-free, powder-free nitrile examination gloves, single use, non-sterile. Tested for use with chemotherapy drugs We've had a terrible time with getting good gloves, and then when we get some good ones, they are replaced the following year with a cheaper brand until our PAs start screaming. You're right - Surgery wouldn't even have to whistle!! Janet From: histonet-boun...@lists.utsouthwestern.edu on behalf of Robert Richmond Sent: Wed 9/2/2009 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Gloves Janet Bonner notes: We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com.(1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box not intended to be used as a chemical barrier, but they do a great job when exposed to Histology chemicals. Not familiar with this product, but with nitrile rubber gloves brand name is important, as the quality of nitrile rubber gloves worsens. When they first came out and were made in the USA, I could make a pair last for two weeks of grossing. As manufacture moved to the latex-producing countries, nitrile rubber (if that's what they actually are) gloves became no better than latex. At present I use two pairs of gloves when I gross. There's a little-known product called chemotherapy gloves - thick blue latex. I filched a box of these and use them - they last for several days. Obviously re-using gloves offsets the high initial cost of purchase, but that's not good MBA thinking. OSHA says not to handle formaldehyde (and I suppose xylene) with latex gloves, but has not specified an alternative. It's disgraceful how little the powers that be care about the hands of pathologists and histotechnologists. You can bet that if it were nurses who had this problem, it'd get solved in a hurry. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Ink issues
Maybe it is the acetic acid in Bouin's that is the active ingredient for dye adherence! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Wednesday, September 02, 2009 10:49 AM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ink issues I concur with the Samurai Pathologist. Long ago, we used Bouin's but have stopped that procedure and prefer to just blot dry but do also use 3% acetic acid, especially if blotting could potentially damage a delicate tissue. Good luck. Joe Galbraith University of Iowa joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, September 02, 2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ink issues About what to put on tissue to make the marking ink stick to it: I don't use anything. The trick is to blot the surfaces thoroughly dry with a paper towel before you try to put the ink on. Of course, there's nothing you can use that will make ink adhere well to cauterized surfaces (some breast biopsy specimens, LEEP cones, and such. The pathologist can however identify cauterized margins under the microscope.) Because of the picric acid (and because it stains your clothes) Bouin's fixative is unacceptable. So is acetone, which is a serious explosion hazard. If you must use something, use 3% acetic acid (white vinegar diluted half-strength). I've been grossing since 1965, worked on maybe sixty different pathology services. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ink issues (UNCLASSIFIED)
Classification: UNCLASSIFIED Caveats: NONE We use Distilled White Vinegar. I buy it at the grocery store by the gallon. It is the acetic acid in bouin's solution that fixes the ink to the tissue, not the picric acid. I have been using white vinegar for more years than I care to remember. Hope this helps. Diane Gladney Supervisor, Histology Dept. Moncrief Army Community Hospital Ft. Jackson, SC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues Good Morning all- I am having issues getting ink to stick to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a ink stay acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to fix your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Uranyl Nitrate
I am looking for an alternate disposal method for my Uranyl Nitrate. I was quoted at about $3000 from our haz. waste disposal company. I would like to find a cheaper method. I cannot ship it outside the US. Would anyone be able to take it and add it to their waste? I have about 500ml of a 1% aq. solution to get rid of. Please let me know if this might be a possibility, I will pay for service. Thank you!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Does anyone have a good recipe for making up a dissect aid in house? Sheila Adey HT MLT _ New! Open Messenger faster on the MSN homepage http://go.microsoft.com/?linkid=9677405___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Gloves
I was just doing some research on the original question posted of gloves suitable for handling xylene. Nitrile is a synthetic rubber made of acrylnitrile and butadiene, devoid of allergy-causing proteins like in latex and natural rubber. The U.S. Dept. of Energy suggests using nitrile when handling xylenes, Ansell suggests either their laminate film gloves, PVA, or nitrile on their very detailed chart offering technical permeabilty data (for instance, it takes 75 min for xylene to break through their nitrile gloves). Now according to OSHA, nitrile does not provide adequate protection, but gives other glove types that are in agreement with what is suggested by some of the other sites I saw. Some of the sites don't seem to say how THICK these gloves are that they are rating, which I think would be a factor for permeability rate. Most of Ansell's gloves were quite THICK (like for industrial use). Another factor for permeability would be how long xylene will be in contact with your glove, if you change gloves frequently or soon after getting xylene on them it seems you'd be ok. I find nitrile (the flexible thin healthcare ones) to be pretty cost-effective. At least, I've dipped my nitrile-gloved fingers in a xylene bath and when I changed my gloves a few minutes later my skin and the inside of the glove was still dry. References: http://www.aps.anl.gov/Safety_and_Training/User_Safety/gloveselection.html http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf http://www.osha.gov/SLTC/healthguidelines/xylene/recognition.html Regards, Merced --On Wednesday, September 02, 2009 1:53 PM -0400 Bonner, Janet janet.bon...@flhosp.org wrote: It says on the box that these aloe touch nitrile gloves are Latex-free, powder-free nitrile examination gloves, single use, non-sterile. Tested for use with chemotherapy drugs We've had a terrible time with getting good gloves, and then when we get some good ones, they are replaced the following year with a cheaper brand until our PAs start screaming. You're right - Surgery wouldn't even have to whistle!! Janet From: histonet-boun...@lists.utsouthwestern.edu on behalf of Robert Richmond Sent: Wed 9/2/2009 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Gloves Janet Bonner notes: We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com.(1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box not intended to be used as a chemical barrier, but they do a great job when exposed to Histology chemicals. Not familiar with this product, but with nitrile rubber gloves brand name is important, as the quality of nitrile rubber gloves worsens. When they first came out and were made in the USA, I could make a pair last for two weeks of grossing. As manufacture moved to the latex-producing countries, nitrile rubber (if that's what they actually are) gloves became no better than latex. At present I use two pairs of gloves when I gross. There's a little-known product called chemotherapy gloves - thick blue latex. I filched a box of these and use them - they last for several days. Obviously re-using gloves offsets the high initial cost of purchase, but that's not good MBA thinking. OSHA says not to handle formaldehyde (and I suppose xylene) with latex gloves, but has not specified an alternative. It's disgraceful how little the powers that be care about the hands of pathologists and histotechnologists. You can bet that if it were nurses who had this problem, it'd get solved in a hurry. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email.
RE: [Histonet] Cost to produce an HE slide
I don't know what the COST to produce an HE is but we charge outside entities $8 each to stain them. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Amy Johnson Sent: Wed 9/2/2009 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost to produce an HE slide Hello Histonet.Has anyone ever figured out how much it costs to produce an HE slide? Are there any articles out there that would help to figure this out? I realize it may be different for each institution but a ball park figure would be greatly appreciated. Thanks Amylin Johnson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Gloves
We also use the Aloetouch gloves for all aspects of Histology and have had no issues with them. They fit like a glove and are very durable but continue to have good flexability. Luckily Medline is on contract with HPG (our buying group)!!! Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 dawn.schnei...@ministryhealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Wednesday, September 02, 2009 12:54 PM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Gloves It says on the box that these aloe touch nitrile gloves are Latex-free, powder-free nitrile examination gloves, single use, non-sterile. Tested for use with chemotherapy drugs We've had a terrible time with getting good gloves, and then when we get some good ones, they are replaced the following year with a cheaper brand until our PAs start screaming. You're right - Surgery wouldn't even have to whistle!! Janet From: histonet-boun...@lists.utsouthwestern.edu on behalf of Robert Richmond Sent: Wed 9/2/2009 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Gloves Janet Bonner notes: We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com.(1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box not intended to be used as a chemical barrier, but they do a great job when exposed to Histology chemicals. Not familiar with this product, but with nitrile rubber gloves brand name is important, as the quality of nitrile rubber gloves worsens. When they first came out and were made in the USA, I could make a pair last for two weeks of grossing. As manufacture moved to the latex-producing countries, nitrile rubber (if that's what they actually are) gloves became no better than latex. At present I use two pairs of gloves when I gross. There's a little-known product called chemotherapy gloves - thick blue latex. I filched a box of these and use them - they last for several days. Obviously re-using gloves offsets the high initial cost of purchase, but that's not good MBA thinking. OSHA says not to handle formaldehyde (and I suppose xylene) with latex gloves, but has not specified an alternative. It's disgraceful how little the powers that be care about the hands of pathologists and histotechnologists. You can bet that if it were nurses who had this problem, it'd get solved in a hurry. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Endogenous biotin blocking
Sally: The simple answer to the question of whether or not biotin blocking is required is ‘no’. As you stated in your inquiry, blocking need only be performed when excessive nonspecific staining, attributable to endogenous biotin, is observed. For what its worth, I think that some folks believe biotin blocking is required because they've miscontrued information contained within the College of American Pathologists AP checklist, which states: “If the laboratory uses an avidin-biotin complex (ABC) detection system...is there a policy that addresses nonspecific false positive staining from endogenous biotin? As written, this question only implies that an assessment of false positive staining be performed. Therefore, provided that the lab conducts the recommended assessment, documents their results within appropriate policies/procedures, and then incorporates biotin blocking steps where they're needed, it does not have to perform blocking at all times. I'd welcome additonal input if my understanding is incorrect. Regards, Joe Myers, M.S., CT(ASCP) -- Message: 9 Date: Tue, 1 Sep 2009 08:31:56 -0400 From: Sally Price sprice2...@gmail.com Subject: [Histonet] Endogenous biotin blocking To: histonet@lists.utsouthwestern.edu I recently had a discussion with one of my coworkers about the need/requirement for blocking of endoegnous biotin whenever an avidin-biotin detection system is used, and I was hoping that the IHC experts on the histonet might be able to provide us with some feeback. Its been my understanding that blocking is only necessary when one is certain that background staining is caused by endogenous biotin, but maybe I'm off-base here. I look forward to eveyone's input. Sally ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cost to produce an HE slide
The cost around $12 to produce a regularly stained section. The cost to produce a stained section by an very experienced histotech...priceless. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn [lynn.bur...@illinois.gov] Sent: Wednesday, September 02, 2009 2:02 PM To: Amy Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cost to produce an HE slide I don't know what the COST to produce an HE is but we charge outside entities $8 each to stain them. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Amy Johnson Sent: Wed 9/2/2009 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost to produce an HE slide Hello Histonet.Has anyone ever figured out how much it costs to produce an HE slide? Are there any articles out there that would help to figure this out? I realize it may be different for each institution but a ball park figure would be greatly appreciated. Thanks Amylin Johnson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pERK IHC
Does anyone have any suggestions for antibody, protocol, etc., regarding staining FFPE sections with pERK? Any information would be greatly appreciated. Thanks in advance, Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cost to produce an HE slide
Over the years I have not found any published cost calculations that have addressed individual categories that fluctuate constantly in the department and it seems that there are as many models as there are institutions/companies. In the past I have found if I separate costs into a minimum of 4 categories, I can provide a reasonable calculation for the technical component successfully. Full calculation will require that you have a cost for all shared tasks in the department that contribute to the production of the slide (i.e. accessioning, grossing, embedding, cutting) and be able to allocate a portion of that technical cost to the final slide. I have also been asked to add to the calculation to account for items such as Training/Education for staff, taxes/shipping cost and professional fees. I suggest you communicate with Finance to see what detail is required and to capture all costs for your department. I hope this gets you started. Cost Analysis for HE: Staining Materials + Labor + Instrumentation + Company Cost Allocation Materials - Reagents, Stains, Slides, Cover slips Each item will need to be calculated to number of units (slides) per ordered unit (i.e. gallon, quart, pint, milliliters). Can take total monthly cost of all staining material and divide by total slides stained per month. Example: Hematoxylin = 1500 slides stained per 620 ml (specific to slide staining instrument) or 2.42 slides / mL; Hematoxylin is ordered by the pint or 473 mL; Cost per pint = $29.80 or $0.063/mL; Cost per slide = .063 mL / 2.42 slides or $0.026 per slide. This is only for the Hematoxylin Labor – Average salary and benefits for all employees that will perform task. Average labor cost will be divided by time spent to perform task. Example: Average hourly labor cost = $24.00; time spent to perform staining task = 4 hours; number of slides stained in 4 hours = 480 slides; Cost per slide = 24 x 4 / 480 = $0.20 per slide Instrumentation – Cost to purchase plus the cost of repairs and/or maintenance contract or lease/rental cost per year and then the allocation per your institution amortization schedule. Divide by total number of slides stained per year Example: Cost of instrument = $60,000; Amortized over 7 years = 60,000 / 7 = $8572; Cost of service contract per year = $3,000; Total number of slides stained = 100,000; Cost per slide = 8572 + 3,000 / 100,000 = $0.12 per slide. All instruments used in the process will need to be added to the cost calculation (i.e. cover slip instrument). Company Cost Allocation - This could be a variety of additional cost allocated to your department. Air conditioning, lease rental for building, lights, etc. Acquire monthly or year total from Finance. Eample: Allocated casts assigned= $12,000; 100,00 slides per year; Cost per slide = 12000 / 10 = $0.012 per slide William DeSalvo, B.S., HTL(ASCP) Date: Wed, 2 Sep 2009 11:11:50 -0500 From: ajohn...@aipathology.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost to produce an HE slide Hello Histonet.Has anyone ever figured out how much it costs to produce an HE slide? Are there any articles out there that would help to figure this out? I realize it may be different for each institution but a ball park figure would be greatly appreciated. Thanks Amylin Johnson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Windows Live: Make it easier for your friends to see what you’re up to on Facebook. http://windowslive.com/Campaign/SocialNetworking?ocid=PID23285::T:WLMTAGL:ON:WL:en-US:SI_SB_facebook:082009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: anti-GFP antibody
Hi all, Just to update you, I ordered Abcam's ab13970 chicken anti-GFP and tested it out in paraffin embedded sections with HIER for 10 minutes in Dako target retrieval system. I came in with a Dylight 488 secondary from Jackson Immunoresearch at 1 ug / mL. It worked beautifully at 1:500, 1:1000, and 1:2000. I should've titered it even further down. Thanks for all your help, Adam On Thu, Aug 20, 2009 at 1:38 PM, Hobbs, Carl carl.ho...@kcl.ac.uk wrote: I am sure someone has a cunning combination! I use Abcam's ab13970 chicken anti GFP in Pwax sections after HIER. For me, it is very good Image at Abcam or here : http://www.immunoportal.com/index.php Good luck! carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Embedder
We are in the market for a new embedder. What do you suggest? I tried searching the archives but only came up with older comments. Vendors please feel free to contact frank@sdstate.edumailto:frank@sdstate.edu. We may have to go with a used instrument so would like comments on good vendors. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dragon dictating system
Histonet, Has anyone had any experience with the Dragon dictating system? Pro's or Con's? Thanks for your opinions Judy McKinney John Peter Smith Hospital Fort Worth Texas 817-927-1024 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ink issues
We blot the tissue dry, ink specimen and dip into 50% vinegar . Black and green work best, but all colors work. Judy McKinney JPS Hospital Fort Worth Texas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
