Re: [Histonet] mice legs
No, I tend to use my own for work!sorry couldn'r resist midweek madness...;-) On Tue, Sep 15, 2009 at 3:42 PM, Shaw, Sharon shs...@wpi.edu wrote: Good Morning Histo World, I would like to know if anyone is working with mice legs, I have a PI that I work with that wants to process the whole leg, the problem is I need to decal it first and is wondering if the decal will break down the tissue, I think it would he doesn't think so. And if anyone has do this would it be possible to share your protocol with me from decal to processing. Thanks, Sharon- WPI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotechs-Chicago, IL
Good morning Histologists! A hospital that I am recruiting for in the Chicago area has 2 openings for Histotechs on a 1st and 2nd shift schedule, both positions are contract to hire and included medical, dental and vision benefits through Aerotek. The employer is looking for 3+ years of relevant experience and an ASCP certification is required. Please email me directly at jcraw...@aerotek.com if you are interested or would like further details. Thank you for your time! Jen Crawford, CIR Scientific Recruiter Aerotek Scientific Staffing Phone: 847.221.1358 Fax: 847.303.2370 www.aerotek.com http://www.aerotek.com Please do not keep me a secret...a referral is the best compliment that I can receive! This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] air quality in the lab
that's a good one. I'd like to know that one, too. --On Wednesday, September 16, 2009 10:40 AM -0400 godsgal...@aol.com wrote: Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job In FL
Good Morning! Allied Search Partners is currently accepting resumes for our client in Melbourne, FL area. We are prescreening for the following: Position(s): Histotechnologists/Histotechnicians Shift: 1. One tech needed for Day Shift Full Time (8am-4:30pm) 2. One tech needed for Part Time Please submit resume for prescreening purposes to aly...@alliedsearchpartners.com *All inquiries are kept confidential* Be sure to visit our website to submit a job search request, refer a friend for $$Cash Bonus, and submit your resume to one of our career advisors. Other positions in Florida: Dover, FL (Evening and Overnight Shift Available) -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] air quality in the lab
From the recesses of my sometimes foggy memory, the figure of 4 exchanges/hour has popped up. Perhaps somebody else has some other figure. René J. --- On Wed, 9/16/09, Merced M Leiker lei...@buffalo.edu wrote: From: Merced M Leiker lei...@buffalo.edu Subject: Re: [Histonet] air quality in the lab To: godsgal...@aol.com, histonet@lists.utsouthwestern.edu Date: Wednesday, September 16, 2009, 11:11 AM that's a good one. I'd like to know that one, too. --On Wednesday, September 16, 2009 10:40 AM -0400 godsgal...@aol.com wrote: Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] air quality in the lab
Our last inspection our exchange rate tested out at 52/hour. We had increased from 26/hour because we could still had fumes in the lab. (CMS inspector could smell xylene from our stainer) For the life of me cannot remember what the min. rate is . Tom Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of godsgal...@aol.com [godsgal...@aol.com] Sent: Wednesday, September 16, 2009 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] air quality in the lab Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] air quality in the lab
I seem to remember 12... j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, September 16, 2009 12:18 To: godsgal...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] air quality in the lab Our last inspection our exchange rate tested out at 52/hour. We had increased from 26/hour because we could still had fumes in the lab. (CMS inspector could smell xylene from our stainer) For the life of me cannot remember what the min. rate is . Tom Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of godsgal...@aol.com [godsgal...@aol.com] Sent: Wednesday, September 16, 2009 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] air quality in the lab Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TISSUE FREEZING MEDIUM
I'm having a great deal of trouble finding a tissue freezing medium that is anything like the original OCT -15-25. Since OCT is no longer available at different temperature uses, I cannot cut a good section in our cryostat(-25)without using freezing spray. Even after trying a few different brands of medium,without freezing spray, our sections are still folded,wavy,and all around not of good quality like we used to have. Most of the different brands are fine for a specimen that was just snap frozen and cut, as in a surgical frozen case, but not good for serial sectioning that may take place 1 to 2 days later, as in a renal bx.We have tried to cut on three different cryostats and had similar results.Surely there are those reading this that are having the same problem.Thanks in advance for your help. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] need pneumocystis control blocks
Does any body know where we can get a few pneumocystis control blocks? Last time I needed a control block for AFB the members really came through. Hopefully someone will have extra pneumocystis blocks also. thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: ImmPACT DAB kit
Many thanks for the replies regarding this reagent. I delayed purchasing this as I was very skeptical: however, I can achieve a 1/5-10 fold dilution factor for my precious low affinity (? thus low dilution factor) Abs. I will not be using ImmPACT chromogen kit routinely as, for the vast majority of Abs, I do not find it neccessary. I have been informed ( courtesy of Histonetters who replied) that Dako offer a similarly sensitive kit. For the record, I routinely use a std manual stABCpx-DAB (DAB in-house prepared)detection system in a Research Lab with very little clinical time-constraints) carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Polarizing filters
The polarizer and analyzer are identical filters, and either of them can be used in either location. One must be between the light source and the slide being viewed. The other must be between the slide being viewed and your eye or camera. I place one filter directly on top of my illuminator. The other is in a filter slide in the microscope column, which can be pushed into the light beam or pulled out of it, but you can also place it directly on top of the slide. You rotate either filter to achieve the polarization effect. I rotate the lower one since the other one is not accessible. These filters cut down the light intensity substantially, so you should use them with maximum brightness of the illuminator, iris diaphram wide open, and with neutral density or any other kinds of filters removed from the light beam, including the blue filter if you normally use one. Polarizing filters can be purchased at any camera store, and some science supply companies sell them. Get good quality glass filters though, not cheap plastic ones. -- From: histonet-boun...@lists.utsouthwestern.edu on behalf of jstaruk Sent: Thursday, September 10, 2009 4:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Polarizing filters Does anyone know where I can find the two appropriate filters (lenses) needed to polarize the congo red and Sirius red stains? I have an Olympus CH-2 that needs to be fitted. I understand I need a polarizer lens and an analyzer lens. Are these two different lenses or the same lens, just in different locations on the microscope? Thank you Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Air Quality
Does anyone know where we can find written regulations regarding air quality in the lab? We start @ 330 am, but the HVAC system doesn't come on until 7. So we are working with just portable fans (or heaters in the winter) those first few hours. Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mib-1
I have to turn to the experts here.? I am having problems with the MIB-1.? I am having an issue with the patient samples working.? We use an alcohol based fixativeit has always worked until recently. I have tried everything I can think of to no avail.? I have decreased the titer, increased the incubation, tried a different retrieval solution, tried a different detection...nothing is working. Anyone else having any issues? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Air changes per hour
OSHA's Occupational Exposure to Hazardous Chemicals in Laboratories; Final Rule (Federal Register January 31, 1990) went into effect May 1, 1990. Section C.4 (f) of Non-Mandatory Appendix A indicates that 4-12 room air changes per hours (ACH) of fresh air are normally adequate if local exhaust systems such as hoods are used as the primary method of control. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Air Quality
Regardless of the regulations, fans will not providce them so the real solution is to have HVAC system running when you start, or do they want to subject you to unsanitary conditions? Have the ever heard of an OSHA intervention or about their fines? René J.? --- On Wed, 9/16/09, Cindy DuBois integrated.hi...@gmail.com wrote: From: Cindy DuBois integrated.hi...@gmail.com Subject: [Histonet] Re: Air Quality To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 16, 2009, 2:25 PM Does anyone know where we can find written regulations regarding air quality in the lab? We start @ 330 am, but the HVAC system doesn't come on until 7. So we are working with just portable fans (or heaters in the winter) those first few hours. Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hyaluronidase for IHC pretreatment
Hello, I saw a protocol using hyaluronidase digestion for IHC pretreatment. But I couldn't find the way how to make this solution. Could anybody help me please? Best regards, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] hyaluronidase for IHC pretreatment
You buy hyaluronidase (freeze-dried) from Sigma or some other vendor and make it up in the appropriate buffer. If my memory serves me, and there have been no major advances in the field, hyaluronidase comes in two flavors, one from bovine testis and one from Streptomyces sp. These are NOT the same so be sure of which one is appropriate for your work..I think the Strep variety is more specific? Geoff Amy Lee wrote: Hello, I saw a protocol using hyaluronidase digestion for IHC pretreatment. But I couldn't find the way how to make this solution. Could anybody help me please? Best regards, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Peloris tissue processor
Dr. Evette Demaisip, Thank you for your posting. We have forwarded this to your Leica representative for the Philippines. They should be in contact with you shortly. Alternatively in future you may also contact histosupp...@leica-microsystems.com for further assistance. Best Regards, Stella Argyriou Global Product Manager Biosystems Division Leica Biosystems Melbourne Pty Ltd 495 Blackburn Road Mt Waverley VIC 3149 Australia Telephone +61 3 9211 7514 Facsimile +61 3 9211 7401 www.leica-microsystems.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of evette demaisip Sent: Thursday, 10 September 2009 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris tissue processor Hi. We're currently setting up a histopath lab. We've purchased Peloris tissue processor and have been test processing tissues under different hour protocols. We encountered problems in the 8-hr and 5-hr protocols.Both produced tissues that were too difficult to section in the microtome. My histotechs showed me tissues that were like brittle plastics. Despite reprocessing, the tissues came out the same in the 8-hr protocol. We reprocessed the 5-hr tissues for 12 hrs, the tissues looked microscopically ok. I suspect the tissues were too thickly sampled and poorly fixed. May ask for feedback/advice on the ff;? 1. Possible reasons? 2. Steps to prevent this in the future? 3. Reprocessing protocol in Peloris. Thank you so much. Dr. Evette Demaisip Pathologist,Philippines Get your new Email address! Grab the Email name you#39;ve always wanted before someone else does! http://mail.promotions.yahoo.com/newdomains/aa/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
