Re: [Histonet] Sakura Embedding
As I recall you don't have to do anything special at the time of embedding, but what is it like for the person grossing? They have to correctly orientate the tissue or the specimen could be ruined? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Anne van Binsbergen wrote: i am - love it!! 2009/11/25 Linke, Noelle nli...@mednet.ucla.edu Is anyone using the Sakura TissueTek AutoTec automated embedding machine in their lab? Do you like it? Thank you! Noelle Noёlle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology Laboratory Medicine David Geffen School of Medicine at UCLA Phone: 310-825-7397 Pager: 97471 nli...@mednet.ucla.edumailto:nli...@mednet.ucla.edu IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] In need of light bulbs
Hi Jim Although we are based in the UK we obtain our replacement bulbs from a company in Chicago called Topbulb. They have a huge range of lamps and bulbs for microscopes, lab equipment and medical equipment. Their prices are competitive and they offer a quick service. I'm sure that if you gave them your model number details their staff would be able to help you. I must add that I have no interest in this lamp supply company. Happy hunting and good luck. Alan Taylor Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter Devon. EX1 3AJ. UK Tel: 044 (0)1392 660132 - Original Message - From: jstaruk jsta...@masshistology.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 24, 2009 10:09 PM Subject: [Histonet] In need of light bulbs Does anyone know what size bulb fits the Shandon embedding station, model #6404? There are two of them at the spigot. Both of mine are burnt out and neither have any numbers on the glass or base! Gracias Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Fwd: RE: [Histonet] Sakura Embedding]
Wanted to share this reply. Original Message Subject:RE: [Histonet] Sakura Embedding Date: Wed, 25 Nov 2009 09:56:56 -0500 From: Durden, Kelley kelleydur...@pathology.ufl.edu To: 'Victor Tobias' vic...@pathology.washington.edu References: 0c96f0bfe078d74c91a1c541d24a6ae4968cd...@emgmb1.ad.medctr.ucla.edu f8332fbe0911250047n617df137pdb17cb57e95d1...@mail.gmail.com 4b0d4032.5040...@pathology.washington.edu Hey Victor, Just went out to CA last month for Sakura training on the VIP 6 and got to look at the dream lab Sakura just set up. Very cool! At that lab they have the Automatic Embedding system. What they do to get proper orientation at the time of grossing is they've developed special cassettes that have, for lack of a better word, baskets. They have a biopsy cassette basket, a basket that could be used for tubular structures (ie vas deferens), they have a basket for larger specimens (uterus etc) and baskets that have rows for breast bx and prostate bx. The whole idea is to maintain orientation all the way through the process. The tissues are oriented in these cassette baskets at the time of grossing. They are loaded onto the processor, then loaded into the auto embedding center. Never having to re orient the samples. Then the baskets are embedded directly into the paraffin wax and are sectioned. You section right through the basket. It is made of a special type of plastic that is sectionable. It is a really cool idea and process to watch. I brought home some samples of the baskets so I could try them here in our lab even though we don't have the auto embedding station. We sectioned through a couple of the different basket varieties and got good results. I'd contact my Sakura rep for some samples so you could try to section with the basket and see if it works for you. We don't have the volume that would necessitate an auto embedder b/c we are a research lab - but if we could justify it I'd love to have it. On another note - we love our VIP 6 processor and the training they sent me for was phenomenal. Hope this helps! Kelley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, November 25, 2009 9:33 AM To: Anne van Binsbergen Cc: histonet@lists.utsouthwestern.edu; Linke, Noelle Subject: Re: [Histonet] Sakura Embedding As I recall you don't have to do anything special at the time of embedding, but what is it like for the person grossing? They have to correctly orientate the tissue or the specimen could be ruined? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Anne van Binsbergen wrote: i am - love it!! 2009/11/25 Linke, Noelle nli...@mednet.ucla.edu Is anyone using the Sakura TissueTek AutoTec automated embedding machine in their lab? Do you like it? Thank you! Noelle Noёlle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology Laboratory Medicine David Geffen School of Medicine at UCLA Phone: 310-825-7397 Pager: 97471 nli...@mednet.ucla.edumailto:nli...@mednet.ucla.edu IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax
Re: [Histonet] (no subject)
I'd be curious to see how you have your processor times set for each station. I'm sure you're using a different setting on the weekends to account for the longer time. Have you made sure that all the other stations are getting the same time they normally get during the week? Drew On Wed, Nov 25, 2009 at 10:13, Taylor, Robin robin.tay...@prexushealth.com wrote: We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
I keep hearing that the guidelines will be changed to 6-72 hours. Therefore, I wouldn't make any changes right now unless you are getting suboptimal results. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Mike Pence mpe...@grhs.net 11/25/2009 11:03 AM Side note question - How are you dealing with the timing issues with breast specimens being fixed in formalin for greater than 48 hr in regard to her-2 requirements with not working Saturdays? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Robin Sent: Wednesday, November 25, 2009 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
We don't work on Saturdays, either. Our pathologists come in on Sunday to gross, so they take the breast specimens off of the processor and place them in a plastic container. On Monday morning, we melt them down and embed and cut them. We have three processors and one is set up for breast and/or fatty specimens. I have a separate program for the weekend. I extend the times in all of the solutions, especially xylene and paraffin, to improve the overall processing of the breast specimens. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, November 25, 2009 8:04 AM To: Taylor, Robin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Side note question - How are you dealing with the timing issues with breast specimens being fixed in formalin for greater than 48 hr in regard to her-2 requirements with not working Saturdays? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Robin Sent: Wednesday, November 25, 2009 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Biopsy fixation
Kris, why don't you leave the skin biopsies in NBF over night? At our institute it is usual practice to let this specimens fix until next day, if they arive after 1 p.m. I cannot see a negative effect. For me 4 hours in formalin are too less for a good fixation. How long is the duration of fixation in the infiltration processor? Aren't the biopsies rather brittle while cutting, due to secondary fixation with ethanol? Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Kalleberg, Kristopher Gesendet: Mittwoch, 25. November 2009 00:51 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Biopsy fixation Hello All, I have a simple fixation question. Typically when we process 3 mm skin biopsies we will fix in 10% NBF for 4 hours and then wash in PBS and then process over night into paraffin and then embed. If we are unable to immediately process the 3mm skin biopsies after 4 hr fixation what is the maximum time we can leave the biopsies in 70% alcohol before processing into paraffin? Is there a better reagent, such as PBS, other than alcohol to leave the biopsy in for a few days before tissue processing. Thank you in advance. Kris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] weekend proc. fix -- a simple idea
hi Robin- IThere are a dozen ways to address this but a very simple one without extra resources might be this: Set your processor with the extra 24-48 hours held in the first alcohol. You probably don't want to spread the time across the whole machine or your end product will be VERY different than your weekly processing. 70% doesn't harden the biopsies and meets the fixation requirements currently under regulations. This works if you don't add to the processors after they're first loaded--set your end time for Monday morning and go! Once they change the reg to 6-72 hours, then rethink the distribution of time. All the other solutions are valid, but his way you don't have to obligate the pathologists to extra handling or have to deal with more than one processor and it's still inside current regs. Happy Thanksgiving! Cheryl Kerry, HT(ASCP) Full Staff Inc. 800.756.3309 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: RE: [Histonet] (no subject)
Are they cutting the pieces extra thick because they are sitting over the weekend, perhaps? Even if the tissues are well fixed if they are too thick they will not process well, especially breast fat. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, November 25, 2009 9:28 AM To: Mike Pence; histonet@lists.utsouthwestern.edu; Robin Taylor Subject: SPAM-LOW: RE: [Histonet] (no subject) I keep hearing that the guidelines will be changed to 6-72 hours. Therefore, I wouldn't make any changes right now unless you are getting suboptimal results. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Mike Pence mpe...@grhs.net 11/25/2009 11:03 AM Side note question - How are you dealing with the timing issues with breast specimens being fixed in formalin for greater than 48 hr in regard to her-2 requirements with not working Saturdays? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Robin Sent: Wednesday, November 25, 2009 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea
This would work but make sure your tissue is very well fixed 24-48hrs before letting them sit in 70% or they will get alcohol fixed and that can be a problem. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, November 25, 2009 12:34 PM To: robin.tay...@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea hi Robin- IThere are a dozen ways to address this but a very simple one without extra resources might be this: Set your processor with the extra 24-48 hours held in the first alcohol. You probably don't want to spread the time across the whole machine or your end product will be VERY different than your weekly processing. 70% doesn't harden the biopsies and meets the fixation requirements currently under regulations. This works if you don't add to the processors after they're first loaded--set your end time for Monday morning and go! Once they change the reg to 6-72 hours, then rethink the distribution of time. All the other solutions are valid, but his way you don't have to obligate the pathologists to extra handling or have to deal with more than one processor and it's still inside current regs. Happy Thanksgiving! Cheryl Kerry, HT(ASCP) Full Staff Inc. 800.756.3309 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: [Histonet] Biopsy fixation
You can leave it in formalin for 48-72 hours without a problem, it might be better than fixing just for 4 hours, some say adequate formalin fixation takes at least 24 hours no matter the size of the sample. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, November 24, 2009 4:51 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Biopsy fixation Hello All, I have a simple fixation question. Typically when we process 3 mm skin biopsies we will fix in 10% NBF for 4 hours and then wash in PBS and then process over night into paraffin and then embed. If we are unable to immediately process the 3mm skin biopsies after 4 hr fixation what is the maximum time we can leave the biopsies in 70% alcohol before processing into paraffin? Is there a better reagent, such as PBS, other than alcohol to leave the biopsy in for a few days before tissue processing. Thank you in advance. Kris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
