[Histonet] Used microtome
Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi = In ricordo di Nice: E Argo, che aveva visto Odisseo dopo vent’anni, fu preso dal Fato della nera morte. Omero, Odissea, XVII, 290-327 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] shipping slides, elementary question
I pack slides in 100-slide plastic boxes, with gauze sponges laid inside the boxes on top of slide edges to ensure that they don't jiggle around inside the box. Boxes are taped shut, then packed inside a cardboard box with plenty of room for packing peanuts or bubble wrap. Never have had a problem. Jan Shivers - Original Message - From: Nicole Collette collet...@mail.llnl.gov To: histonet@lists.utsouthwestern.edu Sent: Friday, January 08, 2010 12:28 PM Subject: [Histonet] shipping slides, elementary question Happy Friday everyone, I have a very basic question about shipping slides (mouse tissue, non-biohaz). I am planning to ship a bunch to a collaborator, on the order of a few hundred. I have black hinged cardboard/wood 100-slide boxes, similar to https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=48452-001inE=1highlight=48452-001 will these be sufficient for shipping (provided I ensure they stay closed during transit) to avoid breakage? I have slide mailers, but they only hold a few slides. I will do that if I need to (it would be a whole lot of packaging and labeling though...), but don't want to just send a giant box and have them all broken on the other end. Maybe there's another alternative? I'm sure someone on histonet has done this before :) Thanks in advance for the advice. Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley collet...@llnl.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Used microtome
Try looking for microtome on e-bay. There is a company there that sells a Cambridge rocker for a very reasonable price. Bryan Llewellyn - Original Message - From: Massimo max_histo...@yahoo.it To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Sent: Monday, January 11, 2010 8:14 AM Subject: [Histonet] Used microtome Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi = In ricordo di Nice: E Argo, che aveva visto Odisseo dopo vent’anni, fu preso dal Fato della nera morte. Omero, Odissea, XVII, 290-327 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Used microtome
Hi: There are a couple of sources besides the makes and vendors but as always it is buyer beware. eBay - you can go to www.ebay.com and do a search for microtome, right now they have several for sale. The good thing about eBay is the shipping is often reasonable and you can get a good deal. The bad news is that things can be way overpriced and the sellers usually don't know what they are selling and expensive parts can be missing so you really need to know what you are doing before you buy. GoIndustry - DoveBid at http://www.go-dove.com/default.asp This is a very different deal than eBay. Here you are buying products that are surplus when a company closes or gets rid of excess equipment. Please note microtomes are used in ares outside of BioMedical. For example companies that fabricate plastic often use microtomes for testing purposes so you can find them in unexpected places. Problem is that with DoveBid shipping is totally on you - unless you are near the auction site you will need to pay a shipper to pick the item up, pack it up and ship it to you. This can easily cost $300 for a microtome so you need to know what your are doing plus you pay a buyer's premium which can range for 16% to 18%. I find equipment on DoveBid is often newer and looks to be in better shape than eBay but it costs lots more. Right now DoveBid lists 5 different microtomes: Shandon Finesse ME Leica RM2135 Biocut 2035 Reicheit-Jung 2050 Leica RM2155 Leica RM2025 My advice is to take your time and know what you are doing - Good luck in your search. Mike Rio Grande Biological On Mon, 2010-01-11 at 16:14 +, Massimo wrote: Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi = In ricordo di Nice: E Argo, che aveva visto Odisseo dopo vent’anni, fu preso dal Fato della nera morte. Omero, Odissea, XVII, 290-327 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: getting rid of surplus equipment-Source?
Belair Instrument Company, 800-783-9424 They have bought most of my old equipment at a fair price. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph [mada...@medimmune.com] Sent: Thursday, January 07, 2010 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] getting rid of surplus equipment-Source? Does anyone have a good source to get rid of equipment that is still good and maybe our lab can make a few bucks in the process? The last time I did this I was told the processor and stainer I gave away would be going to some charity and of course it did not I found out later. These items are off the books, maybe in need of a small repair. We have no room and the equipment is just been depreciated such that if we can get a credit or something for our lab as a trade in. Specifically we have an RMS microtome in perfect condition used for 6 months and put away. The other is a Leica Embedder that does everyting but dispense paraffin. We were using a separate paraffin dispenser and it was fine, but finally got another embedder. Anyway, vendors welcome. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Used microtome
Try looking in e-Bay. They always have some. René J. --- On Mon, 1/11/10, Massimo max_histo...@yahoo.it wrote: From: Massimo max_histo...@yahoo.it Subject: [Histonet] Used microtome To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 11:14 AM Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi = In ricordo di Nice: E Argo, che aveva visto Odisseo dopo vent’anni, fu preso dal Fato della nera morte. Omero, Odissea, XVII, 290-327 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Used microtome
Hi, I have seen several answer suggesting eBay. Before you go there you need to know what kind of kinives you be using so you get teh correct knife blade holder. Are you using disposable knives? If so are they high or low profile? Are you planning on sharpening solid knives? What kind of block holder do you need? Are you embedding in cassettes or with forms? All of these questions need to be answered before you buy or you could end up with something you can not use or is unusable in your hands. If you know all these answers eBay could be a good way to go. I would try to talk to some of the used equipment people and be sure you are going in the correct direction. Pam Marcum UAMS - Original Message - From: Massimo max_histo...@yahoo.it To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Sent: Monday, January 11, 2010 10:14:54 AM GMT -06:00 US/Canada Central Subject: [Histonet] Used microtome Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi = In ricordo di Nice: E Argo, che aveva visto Odisseo dopo vent’anni, fu preso dal Fato della nera morte. Omero, Odissea, XVII, 290-327 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Counterstain for fluorescent tissues
John, would the neutral red technique work the same with unfixed tissues? The fluorophore Nick uses might not fluoresce after formalin fixation. I suspect he would also need to be sure it's stable after mounting with DPX or other resinous medium. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO Neutral red (CI 50040) is an excellent fluorescent Nissl stain: 0.002%, in water, for 5 minutes; dehydrate, clear, and mount in DPX or another non-fluorescent resinous medium. With excitation by either near-UV or blue light (range 325-500nm) the Nissl substance and nuclei fluoresce yellow-orange. Reference: Allen, D. T. Kiernan, J. A. 1994. Permeation of proteins from the blood into peripheral nerves and ganglia. Neuroscience 59(3):755-764. We used this very dilute neutral red as a fluorescent counterstain for paraffin and cryostat sections of formaldehyde-fixed tissues from rats that had received iv injections of rhodamine-labelled albumin (green excitation, orange-red emission, localization mostly extracellular). Franz Nissl was a man, not a granule, substance or stain, so we should give his surname its capital N. Check out http://www.whonamedit.com. John Kiernan Anatomy, UWO London, Canada = = = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] flattening tissue surface
Hi, I need help to prepare some paraffin tissue slides for confocal microscope evaluation. We need the tissue surface very flattened, no wrinkle in order that the microscope can focus the whole section evenly. Anyone has suggestions? Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Paraffin Disposal
Allison we use tissue prep paraffin and it comes in cardboard containers, we save the empty containers to pour the waste paraffin in. Once it solidifies we dump it in the biohazardous container to be disposed of. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, January 11, 2010 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Disposal Happy New Year to all. How are you all disposing the paraffin that comes off of the tissue processor? Do you consider it as biohazard waste and disposing it as such or are you putting it into the regular trash disposal? I have been asked why I am disposing it the way I do. I consider it as biohazard waste. Your help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eliminating the edge effect in IHC/IF
Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax http://www.prosci-inc.com www.prosci-inc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Podoplanin D2-40
I am using Podoplanin, D2-40 on cytology cases. We use a mesothelioma tissue for the control and the control performs appropriately, but our cell blocks are staining very faintly. We stain on the Ventana Benchmark using the following protocol: Depar, standard CC1, antibody for 32 min, amplify selected, counterstain, and post counterstain. I am thinking the standard CC1 may be too harsh for the cell blocks and maybe cutting it back to mild treatment may help. Any other suggestions to boost the staining intensity of the podoplanin on the cell blocks? Thank you in advance for your thoughts and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eliminating the edge effect in IHC/IF
Is it just unevenly stained? Or are your sections unevenly thick!? Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax http://www.prosci-inc.com www.prosci-inc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] flattening tissue surface
Cut very thin (about 2 µm) and let the sections expand well before taking them from the water bath. After that shake them and put the slides vertical on their smaller side (1 inch) for 15 minutes. Take to the oven at 60ºC for 10 minutes and proceed as usual. You could also buy a Kurabo S200 sectioning robot that has been found to be specially useful for the type of work you are describing (better than manual sectioning). René J. --- On Mon, 1/11/10, Karen Cai k...@prosci-inc.com wrote: From: Karen Cai k...@prosci-inc.com Subject: [Histonet] flattening tissue surface To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 1:54 PM Hi, I need help to prepare some paraffin tissue slides for confocal microscope evaluation. We need the tissue surface very flattened, no wrinkle in order that the microscope can focus the whole section evenly. Anyone has suggestions? Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Disposal
It is incinerated. René J. --- On Mon, 1/11/10, Scott, Allison D allison_sc...@hchd.tmc.edu wrote: From: Scott, Allison D allison_sc...@hchd.tmc.edu Subject: [Histonet] Paraffin Disposal To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 1:24 PM Happy New Year to all. How are you all disposing the paraffin that comes off of the tissue processor? Do you consider it as biohazard waste and disposing it as such or are you putting it into the regular trash disposal? I have been asked why I am disposing it the way I do. I consider it as biohazard waste. Your help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eliminating the edge effect in IHC/IF
Usually that is the result of incomplete fixation. Check your fixation protocol. René J. --- On Mon, 1/11/10, Karen Cai k...@prosci-inc.com wrote: From: Karen Cai k...@prosci-inc.com Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax http://www.prosci-inc.com www.prosci-inc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cassette bar coding and label printing
We are looking for a system to 2D barcode our cassettes and then at the histology bench with the aide of a 2D barcode reader print labels for the slides. We would like to utilized our current LIS systems. My question to histoland What systems are you currently using? Do you like it and is it user friendly and cost effective? Do you see a reduction in errors due to the implementation of the system? Do you see a slow down in production due to the application? Thank you in advance for the replies and information. mad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Counterstain for fluorescent tissues
Thanks for the help - I could just mount in hydromatrix or similar and view immediately without dehydration. I will try this and will also use DAPI and prepare an HE on the adjacent slide to show alongside it. Thanks again for the advice, Nick -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Monday, January 11, 2010 10:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Counterstain for fluorescent tissues John, would the neutral red technique work the same with unfixed tissues? The fluorophore Nick uses might not fluoresce after formalin fixation. I suspect he would also need to be sure it's stable after mounting with DPX or other resinous medium. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO Neutral red (CI 50040) is an excellent fluorescent Nissl stain: 0.002%, in water, for 5 minutes; dehydrate, clear, and mount in DPX or another non-fluorescent resinous medium. With excitation by either near-UV or blue light (range 325-500nm) the Nissl substance and nuclei fluoresce yellow-orange. Reference: Allen, D. T. Kiernan, J. A. 1994. Permeation of proteins from the blood into peripheral nerves and ganglia. Neuroscience 59(3):755-764. We used this very dilute neutral red as a fluorescent counterstain for paraffin and cryostat sections of formaldehyde-fixed tissues from rats that had received iv injections of rhodamine-labelled albumin (green excitation, orange-red emission, localization mostly extracellular). Franz Nissl was a man, not a granule, substance or stain, so we should give his surname its capital N. Check out http://www.whonamedit.com. John Kiernan Anatomy, UWO London, Canada = = = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Podoplanin D2-40
Are your fluids initially fixed in alcohol before the cell block is prepared? If so, that can reduce immunoreactivity for some proteins. If you can, I would recommend trying to decrease the sensitivity of your antigen retrieval. I am a strong proponent of Personalized antigen retrieval (adjusting the retrieval based on the type of specimen, fixation, and the length of fixation). I do not have any experience with Ventana's instruments, but on our Bond Max and Bond III instruments, we can adjust the antigen retrieval very easily (low vs. high pH and the time). Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Carol Bryant cb...@lexclin.com 1/11/2010 2:10 PM I am using Podoplanin, D2-40 on cytology cases. We use a mesothelioma tissue for the control and the control performs appropriately, but our cell blocks are staining very faintly. We stain on the Ventana Benchmark using the following protocol: Depar, standard CC1, antibody for 32 min, amplify selected, counterstain, and post counterstain. I am thinking the standard CC1 may be too harsh for the cell blocks and maybe cutting it back to mild treatment may help. Any other suggestions to boost the staining intensity of the podoplanin on the cell blocks? Thank you in advance for your thoughts and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
