[Histonet] Happy Medical Laboratory Professionals Week from Pam Barker at RELIA!
Hi Histonetters! I hope you are doing well. I wanted to send a special bulletin wishing you a Happy National Medical Laboratory Professionals Week. I hope your lab has been having some fun, informative and special events to celebrate because you deserve it.I have heard of some really cool things that labs are doing to mark the occasion including luncheons, costume, trivia and photo contests and displays to educate others about what you do. If you get a chance check out Advance Magazines coverage of Lab Week on Facebook. You dont have to belong to Facebook to see it. Here is the link: http://www.facebook.com/ADVANCEMedLab http://www.facebook.com/ADVANCEMedLab Also I would love to hear what you are doing in your lab to celebrate so please shoot me back an e-mail and let me know. I also wanted to let you know about the great job opportunities I am currently working on. I have supervisor and lead positions in Fresno, California, Boston area, Massachusetts, Las Vegas, NV and Long Island, NY I have histotechnician/histotechnologist positions in Los Angeles, CA,Corpus Christi, TX, Orange/Rockland County, NY, Cape Cod and Boston area of MA. I also have PA positions in Charlotte, NC and Las Vegas, NV. All of my positions are full time permanent positions My clients offer excellent compensation, benefits and relocation assistance. If you or anyone you know might be interested please contact me. I can be reached toll free at 866-607-3542 or at rel...@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fluoro Jade B (or C) protocol
Hi All, Does anyone have an IHC protocol for Fluoro Jade B (or C)? I have read it can be adapted from FITC to IHC but I can't find a protocol. Thanks, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new er pr cap guidelines
Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- Date: Tue, 20 Apr 2010 09:46:43 -0500 From: anita dudley azdud...@hotmail.com Subject: [Histonet] new er pr cap guidelines To: histonet@lists.utsouthwestern.edu Message-ID: snt122-w5922be5ac0a3d838f5b6ebd1...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 has anyone seen the new er pr guidelines for er and pr? my pathologist just came through all in a tizzy about it. just wondering if anyone else has seen it. the fixation times were also suppose to change, to 72 hrs fixation for breast. thanks for anybodies input, anita, providence hosp. mobile alabama -- С уважением, Maxim mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC blocking for liver tissue
Hello, I have a question that what is best way to block endougenous biotin and HRP in liver or kidney tissue. The method I am using has to use avidin-biotin and HRP reactions. This is fine on heart tissue. I used biotin blocking kit from DAKO or Vector. Also I used 1% H2O2. But liver tissue still show background stain. Could you teach me a better way to block or is there other reason cause background? Thanks in advance, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Frozen Rat Heart
Sharon, I used to freeze rabbit hearts (a LITTLE bigger than rat) this way. Pour isopentane into a metal beaker inside a styrofoam container filled with liquid nitrogen to part way up the sides of the beaker. When the isopentane starts to freeze on the bottom of the beaker it is ready. Place cross sections of fresh tissue (or the whole rat heart) on a round of cork with gum tragacanth, orienting it to the way you may be sectioning it if that's its destiny. With a LONG forceps or hemostat, lower cork round, heart side up, into the isopentane. It's important to lower the tissue slowly to prevent cracking but not so slow as to cause freeze artifact. Complete freezing will only take a couple minutes if that. To section tissue, if that's the next step, freeze the bottom of the cork round onto a chuck using OCT. In this way, you insulate the tissue from the RT or cold OCT preventing any chance of thawing of your tissue. Hope this helps you in your situation, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon Sent: Thursday, April 22, 2010 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Rat Heart Does anybody have a protocol on freezing rat heart that you can share with me. Thanks, Sharon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ASCP exam
I was an MLT who transitioned into histology, did the program at IUPUI and passed the HT exam on the first try (Thankfully). I took the exam as soon as could following the program, studied Carson, exams from the program and purchased the online tests through ASCP. My coworker started in the gross room, then moved to cytoprep person and then into histology, did the program at IUPUI and did not pass the first time. We decided she had tried to study TOO many things, and she passed the second time. We talked about the tests and her second test was completely different from the first. I had asked her about the pictures and certain things I had remembered, and she did not have any of that on the first go round. We now have another student who will be sitting for the BOR this summer - we will see where that goes! Nancy Schmitt MLT/HT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Wednesday, April 21, 2010 7:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ascp exam Hello, For those of you who are ASCP certified, either HT or HTL, did many of you have to take the exam more than once? I have not yet sat for the exam, but I see the pass rate is low, and average score is usually just slightly above the pass line. I have read a lot of comments on some message boards saying people don't even recognize the material in a lot of the questions, and how the images of stains are of very poor quality. I am just wondering if most of the people who don't pass are people who think because they work in a lab they will be able to pass the exam, and as a result they don't study for it the first time. Or, is it really that difficult and many people study hard but still cannot pass it. Any comments welcome! Thanks! Brandi Higgins ___ *** NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] reagent alcohol in 50-55 gal drums
Hi All, Does anyone out there purchase their reagent alcohol in 50-55 gal drums? How much does this cost you, including the shipping handling and any other costs? We are trying to determine whether this would be a good idea or not. Thank you, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Toluidine blue
Just be sure you get dye certified by the Biological Stain Commission. The bottle must have a Certification label or it is not certified to perform as expected. Sigma is reliable. Geoff zodia...@comcast.net wrote: Hello to all, I was wondering if anyone knows where to purchase toluidine blue (for mast cells) other than polyscientific. Thank you in advance for your replies, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] protein concentration for Cy2
This question if for anyone doing C4d immunoflourescence staining. Could someone share what protein concentration the Cy2 labeled anti-mouse IgG is prepared at and what buffer is used for that? Our final dilution of the Cy2 is (1:100 in 1%BSA/PBS) but we don't know what the original protein concentration for the dry Cy2 should be. Thank you for your assistance it is greatly appreciated. Eric A. Breisch ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] protein concentration for Cy2
Eric: Are you using Cy2 labeled goat anti-mouse IgG (HL) from Jackson ImmunoResearch Labs? According to their catalog (product number 115-225-003), the stock is 2.0 mg/ml. At 1:100, your working concentration would be 20 ug/ml. Jim B Breisch, Eric ebrei...@rchsd.org Sent by: histonet-boun...@lists.utsouthwestern.edu 04/22/2010 03:51 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] protein concentration for Cy2 This question if for anyone doing C4d immunoflourescence staining. Could someone share what protein concentration the Cy2 labeled anti-mouse IgG is prepared at and what buffer is used for that? Our final dilution of the Cy2 is (1:100 in 1%BSA/PBS) but we don't know what the original protein concentration for the dry Cy2 should be. Thank you for your assistance it is greatly appreciated. Eric A. Breisch ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] need tips for cross-sectioning of cortical bone
Brett- Most wrinkles in decalcified bone sections come from stretching of the decalified bone that occurs during the sectioning process. I would suggest a rather simple solution. Allowing the sections to flatten on the waterbath might take longer or a higher temperature. If paraffin surrounding the bone section seems to be containing it, not allowing it to expand to eliminate the wrinkles, gently tease it off. After all, you want the bone section, not the paraffin. A room temperature water bath (or 30% EtOH) to lay out the sections on to tease out any wrinkles before transfering the sections to the warm waterbath may also help. I hope this helps! Joe Saby, BA HT From: Adam . anonwu...@gmail.com To: Connolly, Brett M brett_conno...@merck.com Cc: histonet@lists.utsouthwestern.edu Sent: Thu, April 22, 2010 11:12:26 AM Subject: Re: [Histonet] need tips for cross-sectioning of cortical bone Cutting bone is very hard, and I'm by no means an expert at it. Assuming the blocks are properly fixed and decalcified, the best thing I've found is to put the blocks at -20C for 5-10 mins to cool them, then right before you cut them, rub a little ice water on the face of the block. That should help you get some nice clean cuts. If the sections become hard to cut again, reapply the ice water. If that stops working, back in the freezer they go. Adam On Thu, Apr 22, 2010 at 9:58 AM, Connolly, Brett M brett_conno...@merck.com wrote: A colleague is having trouble getting wrinkle-free sections of decalcified, paraffin embedded femur. Any tips?? Thanks, Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thank you
Thank you to everyone who responded to my question regarding where to purchase Toluidine blue. It is very much appreciated. Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need IHC Tech in Ny
I need a strong IHC tech with a NY license in Long Island. Great pay. Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
