[Histonet] Positive Controls - IHC
Good day, I am desperately looking for positive controls for Immunohistochemistry testing. I am testing lung tissue for Adenovirus and RSV, but I need a positive control to be able to do this. Since it's not something easily obtained, I tried making cell blocks by using the pellet from a known positive cell culture, but this didn't work. Is it possible to buy known positive blocks and if so, where can I do this? I am new in the field of histology, so I don't know enough to find this out! Thank you in advance! Kind regards, Marilize Burger MScMedSc in Medical Virology Department of Pathology Stellenbosch University Faculty of Health Sciences PO Box 19063 Tygerberg Campus 7505 Cape Town South Africa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new er pr cap guidelines
Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- Date: Tue, 20 Apr 2010 09:46:43 -0500 From: anita dudley azdud...@hotmail.com Subject: [Histonet] new er pr cap guidelines To: histonet@lists.utsouthwestern.edu Message-ID: snt122-w5922be5ac0a3d838f5b6ebd1...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 has anyone seen the new er pr guidelines for er and pr? my pathologist just came through all in a tizzy about it. just wondering if anyone else has seen it. the fixation times were also suppose to change, to 72 hrs fixation for breast. thanks for anybodies input, anita, providence hosp. mobile alabama -- С уважением, Maxim mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new er pr cap guidelines
This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- Date: Tue, 20 Apr 2010 09:46:43 -0500 From: anita dudley azdud...@hotmail.com Subject: [Histonet] new er pr cap guidelines To: histonet@lists.utsouthwestern.edu Message-ID: snt122-w5922be5ac0a3d838f5b6ebd1...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 has anyone seen the new er pr guidelines for er and pr? my pathologist just came through all in a tizzy about it. just wondering if anyone else has seen it. the fixation times were also suppose to change, to 72 hrs fixation for breast. thanks for anybodies input, anita, providence hosp. mobile alabama -- С уважением, Maxim mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new er pr cap guidelines
I am not sure what the problem is here. You have to fix for up to 72 hours no more you can do less time. If you are already fixing your her2 for 48 hours, then what needs to change? You are not going to submit different cassettes for Er/Pr to fix for longer? You will submit them all and fix them all for 48 hours max. The guideline is to prevent under AND over fixation. Not to submit two different samples to fix for different times. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpe...@grhs.net] Sent: Friday, April 23, 2010 10:16 AM To: Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- Date: Tue, 20 Apr 2010 09:46:43 -0500 From: anita dudley azdud...@hotmail.com Subject: [Histonet] new er pr cap guidelines To: histonet@lists.utsouthwestern.edu Message-ID: snt122-w5922be5ac0a3d838f5b6ebd1...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 has anyone seen the new er pr guidelines for er and pr? my pathologist just came through all in a tizzy about it. just wondering if anyone else has seen it. the fixation times were also suppose to change, to 72 hrs fixation for breast. thanks for anybodies input, anita, providence hosp. mobile alabama -- С уважением, Maxim mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new er pr cap guidelines
My problem is that we do not work weekends. If I have a breast that comes in on Friday afternoon, I have to cut it in and have it embedded on Sat. morning. If the guidelines are 72 hr for everything then I can process it on Mondays. -Original Message- From: McMahon, Loralee A [mailto:loralee_mcma...@urmc.rochester.edu] Sent: Friday, April 23, 2010 9:22 AM To: Mike Pence; Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines I am not sure what the problem is here. You have to fix for up to 72 hours no more you can do less time. If you are already fixing your her2 for 48 hours, then what needs to change? You are not going to submit different cassettes for Er/Pr to fix for longer? You will submit them all and fix them all for 48 hours max. The guideline is to prevent under AND over fixation. Not to submit two different samples to fix for different times. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpe...@grhs.net] Sent: Friday, April 23, 2010 10:16 AM To: Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- Date: Tue, 20 Apr 2010 09:46:43 -0500 From: anita dudley azdud...@hotmail.com Subject: [Histonet] new er pr cap guidelines To: histonet@lists.utsouthwestern.edu Message-ID: snt122-w5922be5ac0a3d838f5b6ebd1...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 has anyone seen the new er pr guidelines for er and pr? my pathologist just came through all in a tizzy about it. just wondering if anyone else has seen it. the fixation times were also suppose to change, to 72 hrs fixation for breast. thanks for anybodies input, anita, providence hosp. mobile alabama -- С уважением, Maxim mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
RE: [Histonet] new er pr cap guidelines
Yes, since there is still a difference between ER/Pr and Her2 recommended fixation times (ER/Pr at 72, Her2 at 48 hours) the defacto max time for ER/Pr is still 48 hours. They say they are studying the issue Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Friday, April 23, 2010 7:17 AM To: Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- Date: Tue, 20 Apr 2010 09:46:43 -0500 From: anita dudley azdud...@hotmail.com Subject: [Histonet] new er pr cap guidelines To: histonet@lists.utsouthwestern.edu Message-ID: snt122-w5922be5ac0a3d838f5b6ebd1...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 has anyone seen the new er pr guidelines for er and pr? my pathologist just came through all in a tizzy about it. just wondering if anyone else has seen it. the fixation times were also suppose to change, to 72 hrs fixation for breast. thanks for anybodies input, anita, providence hosp. mobile alabama -- С уважением, Maxim mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ER clone 1D5 or SP1 ?
I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ER clone 1D5 or SP1 ?
We are using 6F11... Sally -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Friday, April 23, 2010 10:17 AM To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ER clone 1D5 or SP1 ? I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new er pr cap guidelines
In those situations you can set up your processors to hold in formalin until the time limit is reached and then switch to 70% Sample comes in on Friday. Place it in formalin (on the processor) until the 48 hours have been reached most likely Sunday afternoon, then switch to an extended 70% alcohol, then continue to process so your cassettes will come off on Monday morning. It might mean that you have a 6-8 hour 70% step, but it should be ok. OF COURSE, run some test tissues first to make sure. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: Mike Pence [mpe...@grhs.net] Sent: Friday, April 23, 2010 10:58 AM To: McMahon, Loralee A; Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines My problem is that we do not work weekends. If I have a breast that comes in on Friday afternoon, I have to cut it in and have it embedded on Sat. morning. If the guidelines are 72 hr for everything then I can process it on Mondays. -Original Message- From: McMahon, Loralee A [mailto:loralee_mcma...@urmc.rochester.edu] Sent: Friday, April 23, 2010 9:22 AM To: Mike Pence; Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines I am not sure what the problem is here. You have to fix for up to 72 hours no more you can do less time. If you are already fixing your her2 for 48 hours, then what needs to change? You are not going to submit different cassettes for Er/Pr to fix for longer? You will submit them all and fix them all for 48 hours max. The guideline is to prevent under AND over fixation. Not to submit two different samples to fix for different times. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpe...@grhs.net] Sent: Friday, April 23, 2010 10:16 AM To: Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- Date: Tue, 20 Apr 2010 09:46:43 -0500 From: anita dudley azdud...@hotmail.com Subject: [Histonet] new er pr cap guidelines To: histonet@lists.utsouthwestern.edu Message-ID:
[Histonet] disposable blade holder
Oh Budget cuts again! I been checking the web for a used disposable blade holder for the Microm HM 310 Microtome with no luck. Does anyone have an extra one laying around that I can purchase cheaply? . ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ER clone 1D5 or SP1 ?
We use the SP1 clone here. Mark Turner, HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mtur...@carisdx.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Contract Histo Tech Needed in Albuquerque, NM
My name is Eric Weber and I am a recruiter for Maxim Government Services. We provide temporary contracted lab personnel to the federal medical facilities. I'm current recruiting for a contract Histology Technologist for an immediate start through August 13th, 2010. Details: * Special consideration given to candidates with previous experience in a Veteran Affairs (VA) Hospital. * Special consideration given to candidate local to the Albuquergue area * Prefer ASCP certification * Monday through Friday, 7am until 3:30 pm * No call, no overtime * Two year experience required cutting and Hematoxylin and Eosin staining. * Housing/travel benefits provide for non local candidates If you are interested please contact me immediately. Please indicate if you have previous experience in a VA Medical Center. Thank You, Eric Weber Maxim Government Services 7227 Lee DeForest Dr Columbia, MD 21046 phone: (410) 910-4942 toll free: (866) 260-9142 fax:(410) 953-8358 erwe...@maxhealth.com CONFIDENTIALITY NOTE: This e-mail message contains confidential, privileged or otherwise protected information intended solely for the addressee. Please do not read, copy, or disseminate it unless you are the intended addressee. If you have received it in error, please call us (collect) at (410) 910-1500 and ask to speak with the message sender. Also, we would appreciate you forwarding the message back to us and deleting it from your system. Thank you. This email and all other electronic (including voice) communications from the sender's firm are for informational purposes only. No such communication is intended by the sender to constitute either an electronic record or an electronic signature or to constitute any agreement by the sender to conduct a transaction by electronic means. Any such intention or agreement is hereby expressly disclaimed unless otherwise specifically indicated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] accessioning of specimens
We seem to be having trouble with the correct printed cassettes being placed on the correct containers. Or wrong numbers printed on cassettes. How does everyone accession their cases? Guidelines for checking cassettes containers? Any suggestions other than looking over their shoulder with each case or going behind them and checking everything? AP assistants handle logging all our cases. (not HT's) Thank you Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cl...@wchsys.orgmailto:joyce.cl...@wchsys.org * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: new DAB
Bless you... Call you tech support and they will come do it for you! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Friday, April 23, 2010 17:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new DAB When did DAKO retire their DAB and only stock the DAB plus? Since it's Friday I can rant. I am so disgusted that no one was notified of the change. With QC I'm going to have to revalidate or prove that the New DAB works as well as or better than the old. Of course I had to be a klutz and spill all but 3 ml of the old DAB buffer and I'll be at a conference all next week with no time to work up a new protocol. My pathologists are going to be upset if their stains don't look right. Don't companies understand we need to know about these changes before they happen? Especially something as common to all labs as DAB! Margaret Perry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gram stain
Now that I'm done with my rant I have a real question. We are trying to do a gram stain on fish and the safranine O is staining everything red. What other stain would you use? I usually have time to look at the books but unfortunately it's almost quiting time and the slides need to be done by noon Monday. Thanks Margaret Perry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thank you all sho gave me advice on reducing background of liver tissue!
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