[Histonet] RE: Are Histotechs considered exempt employees?
From what i know and heard, your facility is definitely getting a great deal. It really depends on the facility. The lab i work at starts non-certified techs around 20. I know of a small derm lab that start non-certified techs around 18. Emmanuel Gonzaga HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Forwarding Request for Control Mehtod
Tony, Is this a universal teenage thing, filthy bedrooms? I'm convinced there are alien life forms in my daughter's bedroom. They make the strangest noises, grunts and groan and I'm bored. Me, I was the perfect teenager apart from the Celtic thing of taking to the drink and of course, lusting after girls. Ian. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 12 May 2010 01:54 To: WILLIAM DESALVO; histonet Subject: RE: [Histonet] Forwarding Request for Control Mehtod Ian, What I have done in the past is to raid the Staff Fridge (or your son's bedroom!). There is usually old food left there that has gone moldy - bread or even strawberries. Wearing gloves in a fume hood slice the bread or fruit into slices (ensuring visible growth of fungi is present), fix for two or more days in NBF, process as usual. Voila - fungi controls Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, 12 May 2010 4:01 AM To: histonet Subject: [Histonet] Forwarding Request for Control Mehtod I wonder if you could help me find someone who would kindly donate a paraffin tissue block containing candida or other fungi to be used as a control block. We have had difficulty in obtaining such tissue and have tried all the hospitals in northern Ireland for a tissue block suitable enough to act as a control. Many of the pathology departments are in the same position as ourselves in that the control tissue being used is getting in short supply. I tried to make one using a culture from microbiology with fresh lambs lung from a local abattoir but unfortunately the tissue seems to stain with PAS as well and the candida where obscured although visible probably due to post mortem changes in the tissue. Alternatively have any of your members successfully produced fungal control material by other means. Thanking you in advance Ian Clarke Biomedical Scientist Cellular Pathology Department Craigavon Area Hospital Sothern Health and Social Services Trust. 66 Lurgan Road Craigavon BT63 5QQ Northern Ireland William DeSalvo, B.S., HTL(ASCP) _ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccountocid=PI D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: FW: [Histonet] Forwarding Request for Control Mehtod
If you have one of those great automatic stainers that has a drain tube going into a sink, you will also find that, after some time, a wonderful fungus will grow inside of the tubing!! We remove the tubing and shake the fungus loose. Counter staining isn't the best on this type of specimen, but when you're in a pinch, you will definitely obtain positive fungus staining! And, wherever you are, it must be warmer/dryer than Michigan right now..please send heat!!! hope this helped! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 Ian Montgomery ian.montgom...@bio.gla.ac.uk 5/12/2010 7:31 AM Tony, Is this a universal teenage thing, filthy bedrooms? I'm convinced there are alien life forms in my daughter's bedroom. They make the strangest noises, grunts and groan and I'm bored. Me, I was the perfect teenager apart from the Celtic thing of taking to the drink and of course, lusting after girls. Ian. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 12 May 2010 01:54 To: WILLIAM DESALVO; histonet Subject: RE: [Histonet] Forwarding Request for Control Mehtod Ian, What I have done in the past is to raid the Staff Fridge (or your son's bedroom!). There is usually old food left there that has gone moldy - bread or even strawberries. Wearing gloves in a fume hood slice the bread or fruit into slices (ensuring visible growth of fungi is present), fix for two or more days in NBF, process as usual. Voila - fungi controls Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, 12 May 2010 4:01 AM To: histonet Subject: [Histonet] Forwarding Request for Control Mehtod I wonder if you could help me find someone who would kindly donate a paraffin tissue block containing candida or other fungi to be used as a control block. We have had difficulty in obtaining such tissue and have tried all the hospitals in northern Ireland for a tissue block suitable enough to act as a control. Many of the pathology departments are in the same position as ourselves in that the control tissue being used is getting in short supply. I tried to make one using a culture from microbiology with fresh lambs lung from a local abattoir but unfortunately the tissue seems to stain with PAS as well and the candida where obscured although visible probably due to post mortem changes in the tissue. Alternatively have any of your members successfully produced fungal control material by other means. Thanking you in advance Ian Clarke Biomedical Scientist Cellular Pathology Department Craigavon Area Hospital Sothern Health and Social Services Trust. 66 Lurgan Road Craigavon BT63 5QQ Northern Ireland William DeSalvo, B.S., HTL(ASCP) _ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccountocid=PI D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Servicing of Leica equipment in the UK
We are a histology lab based in the UK. We have a Leica CV5000 coverslipper and a Leica Autostainer XL. We are trying to find an independent company or operator (other than Leica) than can service our Leica equipment for us. Does anyone know a company or ex Leica engineer we could call? Thanks, Chris m0702...@sgul.ac.uk ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CYP.07680 - cytology cross contamination
Brandi, Body effusions have a high potential for cross contamination during the staining procedure. Body fluids ie: pleural fluid, peritoneal fluid, etc. For that reason they are not stained with other non-gyn specimens ie: bronch. wash, esoph. brush, CSF etc. Also after the staining all solutions up to the 95% before the OG-6 are dumped and refilled. The Hematoxylin, OG-6 and EA65 are filtered. The 95% after the EA-65 is dumped and alcohols are rotated up. This should prevent cross contamination. Written documentation should be your procedure and proof that you are changing/filtering solutions. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Brandi Higgins brandihigg...@gmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 05/11/2010 03:28 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] CYP.07680 - cytology cross contamination Hello all, For CAP policy CYP.07680 for procedures to prevent cross-contamination of specimens during processing and staining - what are your labs doing? I work in a small lab so the histotechs process the cytology specimens and the pathologists read the slides (we have no PA's or cytoprep techs or cytotechs to screen slides). We also process only non-GYN, so we don't have to worry about GYN/non-GYN cross contamination. The notes under this policy say procedures must prevent cross-contamination between highly cellular specimens and suggest the screening method of toluidine blue stain to determing if specimens are highly cellular. Does anyone use the toluidine blue for this purpose? If so could you tell me the procedure for toluidine blue you use? And how do you determing which specimens you stain with toluidine blue and what qualifies as highly cellular. If so do you retain these toluidine blue slides for any period of time? CAP policy also suggests inserting a clean blank slide in each stain run and examine for contamination. Is anyone doing this? We have been inspected before with no problems with this CAP question, but I just want to make sure we are doing everything we can to prevent cross-contamination. Thanks in advance for your input! Brandi Higgins, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Auto Dialer for Thermo Excelsior
Hello Histo, Is there anyone out there with any experience of installing a remote voice dialer on the processor. We have have one and think that it has possibly been fried due to a power surge. However this one was purchased years ago at Radio Shack and they no longer sell the same dialer. The dialers that I currently see are wireless, can anyone out there with experience with these wireless devices give me any suggestions. Thanks in advance, Sylinda Battle, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Good morning, I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot of cells along the way (especially when trying to take the cells out of the tube). We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again. At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing. I am losing a lot of the cells during this step, because the pellet is not quite solid. Do you think it would be OK to let the cells air-dry a bit and then take them out for processing? I know this goes against everything I was taught in histology, but I am really at a loss here. Anyone have any hot tips for me? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene substitutes
What are labs using for xylene substitute. I will be using it in a hand staining setup for Mohs. Feed back on Histo-Clear, Clear solve, S-3 and formular 83. Thanks in advance. Dawn Oakes HT - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Auto Dialer for Thermo Excelsior
Sylinda Go to www.safemart.com and purchase the visonic ltd model dl-125. It is not wireless and works wonderful. We have both our processors hooked to it. Stacey Sent via BlackBerry from T-Mobile -Original Message- From: bsyli...@aol.com bsyli...@aol.com Date: Wed, 12 May 2010 07:15:18 To: histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: [Histonet] Auto Dialer for Thermo Excelsior Hello Histo, Is there anyone out there with any experience of installing a remote voice dialer on the processor. We have have one and think that it has possibly been fried due to a power surge. However this one was purchased years ago at Radio Shack and they no longer sell the same dialer. The dialers that I currently see are wireless, can anyone out there with experience with these wireless devices give me any suggestions. Thanks in advance, Sylinda Battle, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Whatever you do, do not dry them. Perhaps you could follow the same technique as cytotechs use to prepare their cell blocks. René J. --- On Wed, 5/12/10, Kim Merriam kmerriam2...@yahoo.com wrote: From: Kim Merriam kmerriam2...@yahoo.com Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 12, 2010, 9:24 AM Good morning, I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot of cells along the way (especially when trying to take the cells out of the tube). We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again. At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing. I am losing a lot of the cells during this step, because the pellet is not quite solid. Do you think it would be OK to let the cells air-dry a bit and then take them out for processing? I know this goes against everything I was taught in histology, but I am really at a loss here. Anyone have any hot tips for me? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Thanks for the tips everyone! I am going to order some agar (histogel) and use it! We are all very excited around here about this, we have been pulling our hair out over these darn pellets. Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Cormier, Kathleen kathleen.corm...@crl.com To: Kim Merriam kmerriam2...@yahoo.com Sent: Wed, May 12, 2010 10:40:19 AM Subject: RE: [Histonet] (no subject) Hi Kim, Have you used Histogel? I have used it in the past (other places of employment) and on samples like yours. I just bought the gel, (not the whole unit, just the gel) scooped some out of the tube, placed into a weight boat, microwaves for a few seconds until liquid, then took a transfer pipet and dropped onto the cell pellet. I then placed the tube/pellet on a cold plate or fridge and wait until solid. I then popped out the now solid pellet, and processed as usual (no baggie/paper) in a cassette. Works like a charm Kathy Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.corm...@crl.com The Charles River 24th Annual Short Course on Laboratory Animal Science will be held June 21-24, 2010, in Newton, MA. Click Here to get more information on this event. Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, May 12, 2010 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Good morning, I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot of cells along the way (especially when trying to take the cells out of the tube). We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again. At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing. I am losing a lot of the cells during this step, because the pellet is not quite solid. Do you think it would be OK to let the cells air-dry a bit and then take them out for processing? I know this goes against everything I was taught in histology, but I am really at a loss here. Anyone have any hot tips for me? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /PREHRfont size=2 face=ArialThis email, its contents and attachments are confidential and may be covered by legal privilege. This email contains information intended only for the person(s) and/or entity named above. The views and opinions expressed are those of the sender and not necessarily those of Charles River Laboratories or its affiliates. Any other distribution, copying, review, use or disclosure is strictly prohibited. If you are not the intended recipient, please delete this message and any attachments without making a copy and advise the sender by return email - thank you. BR/BRThis email and any attachments have been scanned for viruses prior to leaving the Charles River Laboratories network. Charles River Laboratories will not be liable for direct, special, indirect or consequential damages arising from alteration of the contents of this message by a third party or as a result of any virus being passed on. For more information on the range of services that we offer, please visit our website at BA href=http://www.criver.comhttp://www.criver.com/A/B/fontPRE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
I know the histogel is pretty expensive (1500ish per kit), try ju= st
plain agar powder and mix with water...it is super cheap that way.
Happy spinning!! =)
Sarah Goebel, B.A., HT (ASCP)
H= istotechnician
XBiotech USA Inc.
8201 East Ri= verside Dr. Bldg 4 Suite 100
= Austin, Texas 78744= /em
Original Message
Subject: Re: [Histonet] (no subject)
From: Kim Merriam kmerriam2...@yahoo.com
Date: Wed, May 12, 2010 7:49 am
To: Cormier, Kathleen kathleen.corm...@crl.com
Cc: Histonet histonet@lists.utsouthwestern.edu, sgoe...@xbiotech.co m
Thanks for the tips everyone! I am going to order some aga= r
(histogel) and use it! We are all very excited around here abo= ut
this, we have been pulling our hair out over these darn pellets.
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
= br
_
From: Cormier, Kathleen kathleen.corm...@cr= l.com
To: Kim Merri= am kmerriam2...@yahoo.com
= Sent: Wed, May 12, 2010 10:40:19 AM
Subject: RE: [Histonet] (no subject)
= Hi Kim,
Have you used Histogel? I have used it in the past (other pl= aces of
employment) and on samples like yours. I just bought the gel, (notthe
whole unit, just the gel) scooped some out of the tube, placed
into a w= eight boat, microwaves for a few seconds until liquid, then
took a transfer= pipet and dropped onto the cell pellet. I then placed
the tube/pellet on a= cold plate or fridge and wait until solid. I
then popped out the now solid= pellet, and processed as usual (no
baggie/paper) in a cassette. Works like= a charm
Kathy
Kathy Cormier
Histology Manager
Charles River Laboratories
251 Ballardvale Street
Wilmin= gton, MA 01887
Ph: 781-222-6803
Fax: 978-988-8793
[1]kathleen.corm...@crl.com
The = Charles River 24th Annual Short Course on Laboratory Animal
Science will be= held June 21-24, 2010, in Newton, MA. Click Here to
get more informa= tion on this event.
Accelerating Drug Development. Exactly.
N= otice - This email and any files transmitted with it are
confidential and m= ay contain privileged and/or proprietary
information. You must not disclose= this message to another party
without Charles River's express written cons= ent. If you are not the
intended recipient you must not copy, distribute or= use this email or
the information contained in it for any purpose other th= an to notify
us. If you have received this message in error, please notify
Charles River immediately, and delete it from your system.
-Orig= inal Message-
From: [2]histonet-boun...@lists.utsouthwestern.edu
[mailto:[3]histonet-bounc= e...@lists.utsouthwestern.edu] On Behalf Of
Kim Merriam
Sent: Wednesda= y, May 12, 2010 9:24 AM
To: [4]histo...@lists.utsouthwestern.edu
Subject: [Histonet] (no subje= ct)
Good morning,
I am trying to make some nice FFPE cel= l pellet blocks, but I seem to
lose a lot of cells along the way (espe= cially when trying to take
the cells out of the tube). We are fixing = the cells in NBF,
spinning them down, adding 70% and spinning down again.n= bsp; At
that point, I am trying to scoop out the cells (with a weighing sco op)
and wrap them in lens paper for processing. I am losing a lot of
= the cells during this step, because the pellet is not quite solid.
Do= you think it would be OK to let the cells air-dry a bit and then
take them= out for processing? I know this goes against everything I
was taught= in histology, but I am really at a loss here.
Anyone have any hot t= ips for me?
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge,= MA
__= _
Histonet mailing list
[5]histo...@lists.utsouthwestern.edu
[6]http://lists.utsouthwes= tern.edu/mailman/listinfo/histonet
/PREHRfont si= ze=2 face=ArialThis email, its contents
and attachments are con= fidential and may be covered by legal
privilege. This email contains = information intended only for the
person(s) and/or entity named above. The = views and opinions
expressed are those of the sender and not necessarily th= ose of
Charles River Laboratories or its affiliates. Any other distribution ,
copying, review, use or disclosure is strictly prohibited. If you
are not= the intended recipient, please delete this message and any
attachments wit= hout making a copy and advise the sender by return
email - thank you.
BR/BRThis email and any attachments have been scanned for= viruses
prior to leaving the Charles River Laboratories network. Charles R iver
Laboratories will not be liable for direct, special, indirect or
conse= quential
RE: [Histonet] Are Histotechs considered exemptemployees?
My histotechs are non-exempt employees. In the state of FL, there is a
licensing difference between HTs and HTLs, and their allowable tasks are
different. There is also a pay scale difference, in our facility, between
the HTs and the HTLs. Starting wage is about $3.00 per hour different.
As the supervisor, they have made me an exempt employee.
Michelle
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@xbiotech.com
Sent: Wednesday, May 12, 2010 9:46 AM
To: Kim Tournear
Cc: Histonet
Subject: RE: [Histonet] Are Histotechs considered exemptemployees?
So, I had the same problem a few years ago. They are totall= taking
advantage of you. I live in Austin where I can't imagine tha= the
cost of living is more than CA. The average here is between 20 =nd
25 an hour, sometimes higher if you can get a MOHS job at a priva=e
derm clininc. Start looking, usually in my experience histo. jobs
are everywhere, but sometimes you just have to call around. Also,
usu=ally most places I've noticed don't really seem to care (pay
wise) about HT=versus HTL unless you are wanting to manage a
histology department.
Happy hunting!!
Sarah Goebel, B.A., HT (ASCP)
=iv
Histo=echnician
XBiotech USA Inc.
8201 East Rivers=de Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] Are Histotechs considered exempt employees?
From: Kim Tournear kimtourn...@yahoo.com
Date: Tue, May 11, 2010 7:24 pm
To: Histonet histonet@lists.utsouthwestern.edu
Well said Andi!!
~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson, AZ
~Don't let your life end before it begins~
OU Rocks
--- On Tue, 5/11/10,Heckford,Karen-SMMC-SF
karen.heckf...@chw.edug=; wrote:
From: Heckford, Karen - SMMC-SF karen.heckf...@chw.edu
Subject: RE: [Histonet] Are Histotechs considered exempt employees?
To: Andrea Grantham algra...@email.arizona.edu
Cc: histonet@lists.utsouthwestern.edu
Date: Tuesday, May 11, 2010, 8:17 AM
I am so with you on this one. HT's need to start deman=ing and
higher wages everywhere. We are a rare breed with special talents.
Without a good tech you do not get good slides!! We are not a dime
a=r dozen. AUGH!
I know just a hour north of where I am the wages can be $10-$15.00
less.
Not much difference in living expenses either.
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[[1]mailto:histonet-boun...@lists.utsouthwestern.e=du] On Behalf Of
Andrea
Grantham
Sent: Tuesday, May 11, 2010 7:35 AM
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Are Histotechs considered exempt employees?
Anthony,
You live in CA, work 50 hour weeks and you are a HTL with an
unprecedented and valuable resource at your disposal, Histonet and
you =
know how to use it. For this you get paid $16.15/hour by what
appearsto be an employer who isn't grateful to have you. I'd start
looking
=r for another job.
Just my humble opinion.
Andi
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097
happy slicing and dicing and may all your stains work perfectly -
=r Paula Sicurello
P Please consider the environment before printing this email.
On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote:
Hello fellow Histotechs, I have recently become certified as an
HTLnb=p;
and was wondering if anyone out there is an 'exempt' employee? I
live in California and feel that I am being taken advantage of. I
make 16.15$ per hour and frequently work 50 hour weeks. Am I off
base? should I just be grateful that I have a job, as my employer
son=sp;
frequently reminds me? Thank you Histonet! you have been an
invaluable resource in my career and assisting me in passing the
HTL!=r
___
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___
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Re: [Histonet] Xylene substitutes
We use Formula 83 and are very happy with it. We use it exclusively in the VIP processor and to deparaffinize before staining. We also use as clearing agent after staining, prior to coverslipping, but have found that we still need the last container before coverslipping to be xylene for best results. Otherwise a film develops, and air bubbles are more prominent. I tried switching to another xylene substitute because it was slightly less expensive, but it was not satisfactory and we went straight back to Formula 83. Hope this helps. Brandi Higgins, B.S. HT(ASCP) On Wed, May 12, 2010 at 10:02 AM, Dawn Oakes doa...@olympicmedical.orgwrote: What are labs using for xylene substitute. I will be using it in a hand staining setup for Mohs. Feed back on Histo-Clear, Clear solve, S-3 and formular 83. Thanks in advance. Dawn Oakes HT - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: AMT Cover
To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover. Dr. Tresa Goins Department of Livestock Bozeman, MT 59718 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FFPE cell pellet prep
Here's how I do it. I hate the lens paper method. I transfer suspended cells from a T75 flask to a 50 ml Falcon tube and centrifuge at 400 X g for 5 minutes. Aspirate most of the media so that cells plus media are about 1 ml. Transfer cells plus media to a 1.7 ml microcentrifuge tube. Pellet cells at 400 x G for 5 minutes. Rinse with PBS if needed. Suspend cells in NBF and fix over night. Next day, pellet cells and resuspend them in a small amount (100-200 microliters) of Histogel or Agar. When the gel cools cut the microcentrifuge tube open with a scalpel or razor blade. Now you have a nice cell pellet shaped like the bottom of your centrifuge tube, and you can treat it just like any piece of tissue. Pop it in a tissue processing cassette and then to your ethanol dehydrating steps. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Wed, 12 May 2010 06:50:14 -0700 From: kmerriam2...@yahoo.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FFPE cell pellet prep Sorry - I forgot to put a subject line. Good morning, I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot of cells along the way (especially when trying to take the cells out of the tube). We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again. At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing. I am losing a lot of the cells during this step, because the pellet is not quite solid. Do you think it would be OK to let the cells air-dry a bit and then take them out for processing? I know this goes against everything I was taught in histology, but I am really at a loss here. Anyone have any hot tips for me? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] xylene substitutes
We use formula 83 for everything and do not have a problem with a film. We have found that we must use isopropal alcohol instead of reagent alcohol in the final dehydration steps. Margaret Perry SDSU ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: AMT Cover
What on earth were they thinking?! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Goins, Tresa Sent: Wednesday, May 12, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: AMT Cover To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover. Dr. Tresa Goins Department of Livestock Bozeman, MT 59718 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: AMT Cover
We felt offended by the cover also Even my Cytotechs felt that wasn't necessary!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, May 12, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: AMT Cover To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover. Dr. Tresa Goins Department of Livestock Bozeman, MT 59718 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: AMT Cover
How do we see the cover on line? We did not receive a copy. Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.br...@northside.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Wednesday, May 12, 2010 2:21 PM To: 'Goins, Tresa'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: AMT Cover What on earth were they thinking?! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Goins, Tresa Sent: Wednesday, May 12, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: AMT Cover To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover. Dr. Tresa Goins Department of Livestock Bozeman, MT 59718 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: AMT Cover
So how can I see this cover? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Wednesday, May 12, 2010 3:08 PM To: 'wanda.sm...@hcahealthcare.com'; tgo...@mt.gov; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: AMT Cover Greetings to all. I am SO SO happy to see that others felt the same way about the catalog cover. I immediately went to the AMT website and gave them my opinion. They could definitely have choreographed a more sensitive image. I received a very nice email back, apologizing for any offense, of course un-intentional. The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening. I hope they can make better marketing decisions in the future. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) jander...@halozyme.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Wednesday, May 12, 2010 11:22 AM To: tgo...@mt.gov; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: AMT Cover We felt offended by the cover also Even my Cytotechs felt that wasn't necessary!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, May 12, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: AMT Cover To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover. Dr. Tresa Goins Department of Livestock Bozeman, MT 59718 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: AMT Cover
I had this catalog on my desk and everyone that came in to Histology was astounded by the cover. No need for the dorsal lithotomy position! Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2010 Louisiana Society for Histotechnology State Meeting - Reservation Extension!
Hello Histonetters, The Louisiana Society for Histotechnology would like to invite you to our annual Symposium/Conference on June 4 5, 2010, in Baton Rouge, LA. Meeting location: The Embassy Suites Hotel 414 Constitution Ave. Baton Rouge, LA 70808 We have a block of rooms reserved for attendees at the special rate of $99.00. The cut-off date for reservations has been extended to May 20, 2010. Following that date the rooms may be reserved at that rate per availability. The hotel will honor this rate two days prior and two days after our meeting (please contact the Embassy Suites Hotel for further information: 1-225-924-6566 or 1-800-Embassy). A complimentary hotel shuttle service is provided for those flying into Baton Rouge. Great dining, beautiful southern plantations and gambling boats on the Mississippi River are just a few of the attractions in the Baton Rouge area. Remember to ask for the Louisiana Society for Histotechnology group when placing your reservation. Walk-ins are always welcome! If you have any questions please contact: Tina Van Meter at 225-603-0953 or vanme...@pbrc.edu. Workshops: WS #1: FISH - IT'S NOT JUST FOR DINNER! Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque OSU Medical Center WS #2: What is the Tumor Registry? Cynthia Boudreaux, LPN, CTR Touro Infirmary WS #3: Veterinary vs. Clinical - Which Career is Best for Me? Pam Marcum, B.S., M.S., HT, (ASCP) UAMS WS #4: Microtomy, It's About Technique! Mari Ann Mailhiot, BA, HT (ASCP) Leica Microsystems WS #5: Boot Camp for Histotechs Mari Ann Mailhiot, BS, HT (ASCP) Leica Microsystems WS #6: So You Want to Know More About Things Your Lab Doesn't Do Pam Marcum, B.S., M.S., HT (ASCP), UAMS Bonnie Whitaker, HT (ASCP) QIHC OSU Medical Center WS #7: Dilution, Titrations, and Good Pipetting in the IHC Laboratory Robin Simpkins, HT (ASCP) Biocare Medical WS #8: Detection Chemistry Antibody Production Tania Ewing-Finchem, HT (ASCP) Ventana Roche Hope to see you in June! Tina LSH Secretary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gastric biopsies
Hello Histoland, I was wondering if anyone out there does stains other than HE 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene substitutes
My hand staining setup contained some commercial naphta mix, which is comparable to any other petroleum benzine solvant, for dewaxing. For dehydrating/coverslipping, I used n-Butyl acetate a.k.a. butyl ethanoate. Coverslipping can be done with any xylene soluble glue. What are labs using for xylene substitute. I will be using it in a hand staining setup for Mohs. Feed back on Histo-Clear, Clear solve, S-3 and formular 83. Thanks in advance. Dawn Oakes HT - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Forwarding Request for Control Mehtod
Ah yes, I remember, actually I don't. It was a bit of a blur!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Wednesday, 12 May 2010 9:32 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Forwarding Request for Control Mehtod Tony, Is this a universal teenage thing, filthy bedrooms? I'm convinced there are alien life forms in my daughter's bedroom. They make the strangest noises, grunts and groan and I'm bored. Me, I was the perfect teenager apart from the Celtic thing of taking to the drink and of course, lusting after girls. Ian. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 12 May 2010 01:54 To: WILLIAM DESALVO; histonet Subject: RE: [Histonet] Forwarding Request for Control Mehtod Ian, What I have done in the past is to raid the Staff Fridge (or your son's bedroom!). There is usually old food left there that has gone moldy - bread or even strawberries. Wearing gloves in a fume hood slice the bread or fruit into slices (ensuring visible growth of fungi is present), fix for two or more days in NBF, process as usual. Voila - fungi controls Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, 12 May 2010 4:01 AM To: histonet Subject: [Histonet] Forwarding Request for Control Mehtod I wonder if you could help me find someone who would kindly donate a paraffin tissue block containing candida or other fungi to be used as a control block. We have had difficulty in obtaining such tissue and have tried all the hospitals in northern Ireland for a tissue block suitable enough to act as a control. Many of the pathology departments are in the same position as ourselves in that the control tissue being used is getting in short supply. I tried to make one using a culture from microbiology with fresh lambs lung from a local abattoir but unfortunately the tissue seems to stain with PAS as well and the candida where obscured although visible probably due to post mortem changes in the tissue. Alternatively have any of your members successfully produced fungal control material by other means. Thanking you in advance Ian Clarke Biomedical Scientist Cellular Pathology Department Craigavon Area Hospital Sothern Health and Social Services Trust. 66 Lurgan Road Craigavon BT63 5QQ Northern Ireland William DeSalvo, B.S., HTL(ASCP) _ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccountocid=PI D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
Re: [Histonet] Gastric biopsies
We do Diff Quik on all gastric biopsies, and we do Alcian Blue on all esophageal biopsies with Barrett's indicated by the GI Dr. Our other special stains are by request, but we almost always end up doing retic and trichrome on liver bx submitted with hepatitis as Clinical Dx. Hope this helps. Brandi Higgins BS, HT(ASCP) On Wed, May 12, 2010 at 5:57 PM, Cristi stephenson cls71...@sbcglobal.netwrote: Hello Histoland, I was wondering if anyone out there does stains other than HE 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Gastric biopsies
We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies. Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori. We occasionally did H. Pylori using immunohistochemistry. We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies. Hope this helps. Susan Plaza, HT (ASCP), QIHC -Original Message- From: Cristi stephenson cls71...@sbcglobal.net To: histonet histonet@lists.utsouthwestern.edu Sent: Wed, May 12, 2010 5:25 pm Subject: [Histonet] Gastric biopsies Hello Histoland, was wondering if anyone out there does stains other than HE 's on specific issues. For example, does anyone run stains to rule out H. pylori on all astric biopsies? Any information as to the standards in other labs would be reat. hanks, risti __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
