[Histonet] Re: Higgins ink
Allison Scott HT(ASCP), Histology Supervisor, LBJ Hospital, Houston, Texas asks: Our [pathologist's assistant] used Higgins ink (india ink) on some skin specimens and set the ink with 10% acetic acid solution. After the specimens were processed, all of the ink was gone, washed off. We have regular alcohols and xylene on the processor, this should not have made the ink wash off. Any suggestions on why it washed off, how to prevent this from happening again. My guess is that you got the wrong kind of India ink - there are several varieties for special purposes, and I don't understand this issue very well. I've often bought my own Higgins india ink at art supply stores, always getting the plainest variety I could, and never had trouble with it - but I should learn the specifications better. I never fix ink with anything - if you blot the specimen good and dry, the india ink (or Davidson's ink) will adhere quite well. If you do prefer to use a fixative, use dilute acetic acid - acetone is a fire hazard, and we all know the dangers of the picric acid in Bouin's fixative. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Autotechnicon?
Do you want to sell your old Autotechnicon ? I'll buy any mono or duo Autotechnicon . From: aalli...@wpalabs.com To: histonet@lists.utsouthwestern.edu Date: Wed, 26 May 2010 08:56:02 -0700 Subject: RE: [Histonet] Autotechnicon? My God Tom, I haven't used an Autotechnicon or Ultra-Autotechnico= since 1979! Was it an Mono or a Duo, or the old black spider? =m sure you know what I mean Tom, 1 or 2 levels. I guess ='s better than working in China, reusing their stained slides by sca=ng off the tissue. Akemi Allison BS, HT (ASCP) HTL Manager, Anatomical Pathology Western Pathology Associates Tele: 602.633.3800 X 306 Fax: 602.861.3500 Cell: 408.335.9994 E-Mail: [1]aalli...@wpalabs.com Original Message Subject: Re:=istonet] Autotechnicon? From: Victor Tobias [2]vic...@pathology.washington.edu Da=: Tue, May 25, 2010 12:22 pm To: [3]histo...@lists.utsouthwestern.edu Bob, It= time for you to find a new agent, to get some more modern assignments. Victor Victor Tobias Clinical Applications Analyst Unive=ity of Washington Medical Center Dept of Pathology Room BB220 1959 = Pacific Seattle, WA 98195 [4]vic...@pathology.washington.edu 206-598-2792 206-598-7e9 Fax = === 3D==D== Privileged, confidential or patient identifiable information m= be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, o r if the message has been addressed to you in error, do not read, dis=ose, reproduce, distribute, disseminate or otherwise use this transmis=on. Instead, please notify the sender by reply e-mail, and then destro=ll copies of the message and any attachments. On 5/25/2010 11=6 AM, Robert Richmond wrote: A recruiter, Melissa Ribeiro Passos,=tes that Brine Group is currently recru=ing around a day Histotechnologist position at a hospital-based clinical =b in southeastern Connecticut. Duties would include g= Loading surgical specimens in Autotechnicon an=etting/checking time setting. Are they �tually using an ancient Technicon processor, or is this just loca=ab jargon for a tissue processor? Reason I ask is, I'm c=rently working in a lab that's using a 1964 Technicon, for proces=ng and staining. No vacuum anywhere. It's obvious from looking at=e slides that temperature control is inadequate, and the stainin=s quite capricious. Bob Richmond Samurai Patholo=st Knoxville TN _= Histonet mailing list [5]histo...@lists.utsouthwestern.edu =6]http://lists.utsouthwestern.edu/mailman/listinfo/histonet BR ___ Histonet=iling list [7]hist...@lists.utsouthwestern.edu [8]http://lists.utsouthwestern.�u/mailman/listinfo/histonet References 1. 3Dmailto:aalli...@wpalabs.com; 2. 3Dmailto:vic 3. 3Dmailto:histo...@lists.utso 4. 3Dmailto:vic...@pathology.washi 5. 3Dmailto:Hist 6. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet; 7. 3Dmailto:Histonet@lists.utsouthwestern.edu; 8. 3Dhttp://lists.utsouthwestern.= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vibrotoming otoliths
I am trying to section an otolith from a small minnow species with a vibrotome. The bone seems to be very dense and doesn't hold very well inside any mounting media that I have tried. Does anyone have any suggestions as to a media that will section smoothly but would be dense enough to hold the small bone in the media while its being sectioned. Thank you in advance. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Region II- extension
Histonetters- Region II has extended the mail-in registration deadline, until Friday May 28th...just a few days away! We know some people are still awaiting travel approvals and we are doing our best to accomodate everyone who wants to attend and take advantage of the early registration. I went to the beach last week-end and it was really great...So plan your own Learn and Play weekend at the Clarion with us! For more info go to: www.nsh.org/content/region-ii-meeting http://www.nsh.org/content/region-ii-meeting for all the information on the meeting, speakers, vendors and activities available. See you there! C. Barone - Region II Director. PS: Have anything you want to tell me, or ask me to do to help you with, in your state, or at the national level? Contact me at: cbar...@nemours.org mailto:cbar...@nemours.org and... NSH Members...don't forget to cast your electronic ballots! It is so very easy! Your voice will be heard! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AlphaSMA staining
Hello everyone, We are trying to stain alpha smooth muscle actin (on mouse/human liver) using the A5228 Sigma antibody and are getting nuclear staining while this stain should be cytoplasmic. Does anyone have experience with nuclear staining of alphaSMA? Is there anything I can do to avoid nuclear staining or can anyone recommend me another good anti- alphaSMA antibody that works on both mouse and human formalin fixed paraffin sections? Thank you very much in advance! Phebe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
