[Histonet] RE: Histonet Digest, Vol 79, Issue 38
For Malika I have the Leica Coverslipper and I use Statlab coverslips. They work great and almost no waste. Hope this helps you ... Audrey Toothman Parma Community General Hospital Parma Ohio -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, June 29, 2010 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Canine bone sectioning trouble (Schneider,Lynda S) 2. RE: Coverslips (Malika Benatti) -- Message: 1 Date: Tue, 29 Jun 2010 12:17:58 -0400 From: Schneider,Lynda S emly...@pathology.ufl.edu Subject: [Histonet] Canine bone sectioning trouble To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 94a0bfce84cb0f4a84c50eac9b02d0a90194232...@hsc-cms01.ad.ufl.edu Content-Type: text/plain; charset=us-ascii Hello out there... We are having trouble sectioning canine bone. The samples are bone marrow cubes approximately 2cm thick and 1in wide. They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier. They were faced in and then surface decaled again for about 30 mins. When sectioned, much of the marrow was missing and the bone was torn and shredded. We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again. The samples were then reprocessed as they had been originally. The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so. This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up. If sections are even obtainable they are of hor! rible quality. Does anyone have any suggestions? Thank you so much in advance! Lynda -- Message: 2 Date: Tue, 29 Jun 2010 17:53:26 +0100 From: Malika Benatti ben...@gosh.nhs.uk Subject: [Histonet] RE: Coverslips To: histonet@lists.utsouthwestern.edu Message-ID: 4c2a3319.4626.003...@gosh.nhs.uk Content-Type: text/plain; charset=UTF-8 ** Proprietary ** ** Reply Requested When Convenient ** Hi there, We use the CellPath CoverSlips 22x50mm No 1.5 they are great for hand mounting but when used with the Leica CV5030 they are a real pain. most of the CoversSlips get rejected. If anyone use the same equipment, let me know how you are getting one . Cheers, Malika -- Message: 7 Date: Mon, 28 Jun 2010 15:06:08 -0400 From: Weems, Joyce jwe...@sjha.org Subject: [Histonet] RE: Coverslips To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 92ad9b20a6c38c4587a9febe3a30e164015dfd5...@chexcms10.one.ads.che.org Content-Type: text/plain; charset=us-ascii I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete
[Histonet] Anybody running PIN 4 on Bond
Is anybody running the PIN 4 cocktail on Leica's Bond Max on human tissue? We are trying to bring PIN 4 into our lab. I can't seem to get consistent staining. I am using BioCare's PIN 4 antibodies, Mach 2 and Leica's RED Refine detection kit. I have used Leica's recommended times. We have a great positive control that worked wonderfully when first optimized, so I thought this Leica protocol was great. But could not duplicate results. The RED has always been fine. I have had BioCare replace their antibody.(nuclear staining very light, if there) Have changed protocols times as per BioCare's Tech Services. I am at my wits end. Help. What is your protocol? My director really wants this up and running. Thank you, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJUSA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE stainers
We are in the market for a new HE automated stainer and am hoping I get some feedback on this request! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments private at dorothy.l.w...@healthpartners.com Thanks much ahead of time!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE slide cost
I apologize for asking this question since I'm sure it's been discussed before, but I need an answer ASAP. How much does it cost to produce an HE-stained slide from formalin-fixed tissue? We want to give this figure to our new residents and fellows who start tomorrow in order to show them how much it costs the laboratory to produce the tissue that they submit. Thanks! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stainers
We have a refurbished Leica Autostainer XL and are very happy with it. We are a research lab, so our volume is not as much as a hospital histology lab, but we get big projects every now and then and it has paid for itself. If you are interested in purchasing refurbished equipment, please contact me directly (msherw...@partners.org). Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, June 30, 2010 9:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HE stainers We are in the market for a new HE automated stainer and am hoping I get some feedback on this request! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments private at dorothy.l.w...@healthpartners.com Thanks much ahead of time!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H. pylori RTU antibody
I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still some issues. I was wondering if most people are making their own dilutions to get a better stain or if the RTUs are commonplace. I appreciate the feedback! Thanks, Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: bovine viral diarhea antibody
I am looking for a vendor of BVDV antibody for IHC testing. We had used Syracuse Bioanalytical in the past. They seem to be gone. I would appreciate any suggestions. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: [Histonet] HE stainers
we are going to bring new one too for our small lab. any suggestion please. you can send me at k8...@yahoo.com --- On Wed, 6/30/10, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Subject: [Histonet] HE stainers To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Wednesday, June 30, 2010, 4:57 PM We are in the market for a new HE automated stainer and am hoping I get some feedback on this request! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments private at dorothy.l.w...@healthpartners.com Thanks much ahead of time!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] H. pylori RTU antibody
Use the RTU from Biocare. It is clean and easy. You can use it with the Dako Flex Kit. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer [41dm...@gmail.com] Sent: Wednesday, June 30, 2010 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori RTU antibody I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still some issues. I was wondering if most people are making their own dilutions to get a better stain or if the RTUs are commonplace. I appreciate the feedback! Thanks, Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histologist needed in Austin, Texas
We have a full time position open for a HT / HTL (ASCP) registered histologist, days; requires 3+ years experience and strong embedding and cutting skills. The position is at a Level 1 trauma Children's hospital that has been open for 3 years and has state of the art equipment. If you're interested in working in a team environment and would like to work in beautiful, progressive Austin, Texas please apply online at Seton.net, (Dell Children's Medical Center position) For specific questions please contact: Penny Flowers HT (ASCP) Seton Histology Technical Manager 512.324.1000 Ext. 14264 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FW: bovine viral diarhea antibody
VMRD Mouse monoclonals - Clone D89 and clone BA-2 are now working stronger than my previous clone (15C5 from another vendor). Both need heat retrieval. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Burton, Lynn lynn.bur...@illinois.gov To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 30, 2010 9:48 AM Subject: [Histonet] FW: bovine viral diarhea antibody I am looking for a vendor of BVDV antibody for IHC testing. We had used Syracuse Bioanalytical in the past. They seem to be gone. I would appreciate any suggestions. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] H. pylori RTU antibody
Biocare has a rabbit polyclonal anti-HP ab and a mouse monoclonal. Which one do you use? Mark On Wed, Jun 30, 2010 at 8:05 AM, McMahon, Loralee A loralee_mcma...@urmc.rochester.edu wrote: Use the RTU from Biocare. It is clean and easy. You can use it with the Dako Flex Kit. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [ histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer [ 41dm...@gmail.com] Sent: Wednesday, June 30, 2010 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori RTU antibody I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still some issues. I was wondering if most people are making their own dilutions to get a better stain or if the RTUs are commonplace. I appreciate the feedback! Thanks, Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE stainers
Sakura wins hands down Always Empower your Business with BlackBerry® and Mobile Solutions from Etisalat -Original Message- From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Wed, 30 Jun 2010 08:57:38 To: 'histonet@lists.utsouthwestern.edu'histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stainers We are in the market for a new HE automated stainer and am hoping I get some feedback on this request! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments private at dorothy.l.w...@healthpartners.com Thanks much ahead of time!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Procollagen
All - We are currently using Pro-COL1A1 (N17) and was wondering what others are using for a Procollagen for derm applications? Mary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE slide cost
There are so many variables, it all depends on your operating costs i.e. Labor, reagents, maintenance contracts and capitol equipment if applicable. I just completed my cost analysis today and it costs my department approximately $8.15 to produce 1 HE slide, $2.55 each Special and $38.71 for each IHC Antibody. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, June 30, 2010 8:43 AM To: Histonet Subject: [Histonet] HE slide cost I apologize for asking this question since I'm sure it's been discussed before, but I need an answer ASAP. How much does it cost to produce an HE-stained slide from formalin-fixed tissue? We want to give this figure to our new residents and fellows who start tomorrow in order to show them how much it costs the laboratory to produce the tissue that they submit. Thanks! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chilling Tray for Blocks
I am looking for a piece of equipment that is on wheels and has a chilled top surface for chilling blocks. We have a very old one that does not work that was manufactured by Lipshaw. The top surface is completely flat and measures about 18 x 18. We ordered a Chill Tray from Mopec, but the chilling area is recessed so that you can't see the blocks when you're sitting down cutting, and it is also too small of an area. Does anyone know if there is some sort of portable chiller available out there? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE stainers
Easy to use Easy to program Workhorse Well supported by service engineers in my location Prefer tape coverslips - no mess and no waiting for slides to dry Prisma has optional neat half size containers and matching racks for small batches Versatile system Get sakura to demo Always best to see them operational before you decide I'm just a sakura fan Been using them for over 20years with very few disappoointments Annie Empower your Business with BlackBerry® and Mobile Solutions from Etisalat -Original Message- From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Date: Wed, 30 Jun 2010 11:56:11 To: 'anni...@gmail.com'anni...@gmail.com Subject: RE: [Histonet] HE stainers And your reasons are? I am compiling the +'s and -'s in a spreadsheet for documentation to my manager, otherwise she ends up purchasin the lowest cost item!!! Thanks for your time!! Dorothy webb -Original Message- From: anni...@gmail.com [mailto:anni...@gmail.com] Sent: Wednesday, June 30, 2010 11:25 AM To: Webb, Dorothy L; histonet-boun...@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] HE stainers Sakura wins hands down Always Empower your Business with BlackBerry(r) and Mobile Solutions from Etisalat -Original Message- From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Wed, 30 Jun 2010 08:57:38 To: 'histonet@lists.utsouthwestern.edu'histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stainers We are in the market for a new HE automated stainer and am hoping I get some feedback on this request! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments private at dorothy.l.w...@healthpartners.com Thanks much ahead of time!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reducing blood autofluorescence in IF
Hello everyone! I was wondering if anyone knows of an effective method of reducing/ eliminating autofluorescence of blood in IF procedures. Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: H. pylori RTU antibody
I'm getting tons of responses from people about other antibodies that work well, which I appreciate very much... However, I'm curious to hear from someone that's actually using the Dako RTU and having success... Thanks, Drew On Wed, Jun 30, 2010 at 10:41, Drew Meyer 41dm...@gmail.com wrote: I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still some issues. I was wondering if most people are making their own dilutions to get a better stain or if the RTUs are commonplace. I appreciate the feedback! Thanks, Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE slide cost
I agree w/ Cheryl, there are many variables to consider and there is no standard cost for an HE because we, Histotechnology, do not have a standardized process for HE. The calculation requires precise data and will often be higher than you anticipate. I find that most labs, and the vendors, do not account for the total cost. My cost calculation is as follows: (Materials/Reagents + Instrumentation + Labor + Company Allocation) / Unit (i.e. Case/Specimen/Cassette/Slide/Stain) = Cost You will need assistance from your Finance department to acquire many of the costs associated with your instruments, labor and especially the allocation back to your department for shared services. I also feel that Cheryl's cost per slide is definitely in the ballpark. I have worked at labs, small to very large, and the cost per slide ranges in the $8.00 - $10.00 / slide, when all department expenses are accounted and the proper allocation of technical time is applied. William DeSalvo, B.S., HTL(ASCP) From: cmil...@physlab.com To: rcar...@harthosp.org; histonet@lists.utsouthwestern.edu Date: Wed, 30 Jun 2010 11:51:13 -0500 Subject: RE: [Histonet] HE slide cost CC: There are so many variables, it all depends on your operating costs i.e. Labor, reagents, maintenance contracts and capitol equipment if applicable. I just completed my cost analysis today and it costs my department approximately $8.15 to produce 1 HE slide, $2.55 each Special and $38.71 for each IHC Antibody. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, June 30, 2010 8:43 AM To: Histonet Subject: [Histonet] HE slide cost I apologize for asking this question since I'm sure it's been discussed before, but I need an answer ASAP. How much does it cost to produce an HE-stained slide from formalin-fixed tissue? We want to give this figure to our new residents and fellows who start tomorrow in order to show them how much it costs the laboratory to produce the tissue that they submit. Thanks! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Chilling Tray for Blocks
Fill a Tupperware container with water, pop it in the freezer, and tomorrow you will have a portable block chiller, with the blocks at a perfect level for your needs, for 1/100th the price. Not trying to be a smarta$$, but Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Manager Needed!
Slone Partners is seeking an histology manager for our client in South Florida. As the Histology Manager you will lead a team of technologists in a fast paced laboratory and attend to all operational needs for the department. Candidates must have appropriate certifications HT/HTL(ASCP) and 3-5 years of supervisory experience is required in addition to FL licensure. Candidates must possess effective interpersonal, communication, and leadership skills as well as excellent organizational skills. If you meet these qualifications send your resume to Darcy Bloch at dar...@slonepartners.com or call me at 866-870-7434. Thank you, Darcy SLONEPARTNERS DARCY BLOCH - ASSOCIATE DIRECTOR OF RESEARCH Corporate Headquarters 400 Alton Road #2103 Miami Beach, Florida 33139 TOLL FREE: 866.870.7434 DIRECT: 757.271.5821 CELL: 757.761.4773 www.slonepartners.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processors
I have had a different experience with the Peloris processors. Please note Pat's warning to keep a service contract on the instrument. It is essential. We have had a single Peloris processor in our department more than 2 years. The original processor was replaced by a different unit after we had significant trouble with it. The second instrument is showing the same problems as the first. Just to be clear, we use their paraffin, their cassettes, and the usual alcohol and xylene - nothing exotic. WHEN the processor works, it does a beautiful job. However, we have had more that one occasion where the processing cycle has completed with no error codes, and the tissue is totally unprocessed. It is as if the tissue went from formalin to wax. The root cause...could not be determined. Processing problems, compounded with slow service response has left us twice with down times in excess of 5 working days. Leica has to their credit been working hard to prevent this from happening, but when you have a processor that has a reliability rate of 85%...there are issues. We were told that we would be receiving a stronger motor to prevent the plethora of rotary valve errors that we experience, but that has been delayed.We live with fluctuating volumes of solution. It appears to be a system problem. We thankfully have our Sakura VIP for backup - but the capacity is not sufficient for us to use as a replacement processor. My experience with processors was almost exclusively Sakura VIP models for the past 20 years. In that time, it was a rare occurrence for us to call for service. The transition from a reliable workhorse to a temperamental instrument has been difficult, and an experience I will not repeat. Sheila Tapper This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Chilling Tray for Blocks
We actually use a styrofoam box (used for shipping) and it stays cold longer with very little melting. We keep a couple in the -20 freezer. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Wednesday, June 30, 2010 4:01 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Chilling Tray for Blocks Fill a Tupperware container with water, pop it in the freezer, and tomorrow you will have a portable block chiller, with the blocks at a perfect level for your needs, for 1/100th the price. Not trying to be a smarta$$, but Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE stainers
We have the new version of the Leica XL stainer (looks just like the old one!) with the bridge-transfer station coverslipper love it. We had the old stainer for 10 years before getting the new one... and even managed to sell the old one! Michelle On Jun 30, 2010, at 9:57 AM, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: We are in the market for a new HE automated stainer and am hoping I get some feedback on this request! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments private at dorothy.l.w...@healthpartners.com Thanks much ahead of time!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Procollagen
Mary We use the one from Abcam ab64409, it works really nice Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mary Abosso Sent: Wednesday, June 30, 2010 10:21 AM To: Histonet Subject: [Histonet] Procollagen All - We are currently using Pro-COL1A1 (N17) and was wondering what others are using for a Procollagen for derm applications? Mary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Canine bone sectioning trouble
Overall your fixation, decalcification and processing cycle is too short. The sample size is really big, why do you need it so thick? I would first of all let the samples fix longer 24 to 48 hours or I would trim them so that they are about 3-5 mm in thickness. Either way I like fixing bone samples for 24 to 48 hours. I'm not that accustomed to using RDO decal it is hydrochloric acid based and is quicker but even bone that size would take a while to decal. We normally use a formic acid based decal and a sample size that large my take up to 2 weeks to decal. Also to process a sample that size I would be at 4 to 6 hours a station. We frequently process porcine and goat knees in 2 x 3 blocks the samples are quite thick about a cm in thickness and we will process them at 4 to 6 hours a station. My recommendation would be to decal the larger cube of bone for a period of time until you can section through it. I would then cut a 3-4 mm thick piece off the cube and process it at 1 hour per station. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Schneider,Lynda S Sent: Tuesday, June 29, 2010 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Canine bone sectioning trouble Hello out there... We are having trouble sectioning canine bone. The samples are bone marrow cubes approximately 2cm thick and 1in wide. They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier. They were faced in and then surface decaled again for about 30 mins. When sectioned, much of the marrow was missing and the bone was torn and shredded. We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again. The samples were then reprocessed as they had been originally. The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so. This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up. If sections are even obtainable they are of horrible quality. Does anyone have any suggestions? Thank you so much in advance! Lynda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] unscribe
unscribe please, I do not wish to receive emails anymore, thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
