[Histonet] RE: Re water problem
Thanks for the great tip Steve - it works great! Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Weston Sent: Sunday, July 25, 2010 9:50 PM To: histonet list Subject: [Histonet] Re water problem We had this problem when we started using APTS coated slides and left out adhesive from the water bath. A simple solution I have found is to add a single drop of triton x100 (or similar detergent) to my full water bath and mix before heating the water. This reduces the surface tension of the water and allows it to run off the slide. Regards Steve weston Menzies Research Institute Hobart Tasmania, Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Artifacts in histology section
It's very difficult to diagnosis a problem such as this without hands on. But one thing that I noticed years ago that was so simple could cause this. A build up of tissues on the back of the blade holder. Worth a look. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Joseph Saby saby_josep...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 07/25/2010 12:49 PM To Aazath Raj aaz...@hotmail.com, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Artifacts in histology section What you are describing might be microchatter. These will be sharp parallel lines/cracks that run parallel to the knife edge and are only visible under the microscope. The usuall cause is a combination of overprocessing and rough facing that is too aggressive and/or with too dull a blade. Overprocessing makes the tissue very hard and somewhat brittle. The thick sections/dull knife cause the tissue to compress and then release, causing the chatter. The actual danage is in the block face. Once you have the problem in a block, if the tissue is thick enough, you might be able to repeatedly soak the block in ice water and gently (with a fairly sharp knife) reface. With luck, you might be able to get through the damaged block face. Another artifact I have seen is similar, but the chatter appears very blurry. This is usually caused be poor fixation/processing, then oversoaking the blocks after facing. The trick here is to reface the block, then chill it without exposure to water. I've sectioned such blocks after placing them in a freezer to chill them thoroughly. This will help to obtain a section, but may not fix the staining problems that might show up later. Good luck! Joe Saby, BA HT From: Aazath Raj aaz...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Fri, July 23, 2010 11:26:28 AM Subject: [Histonet] Artifacts in histology section Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Special Stain Storage
Hello All, We were recently inspected by CLIA and our inspector noticed that we didn't have reagent storage temperatures written in our Special Stains procedures. We do our stains by hand so we do have some stored in the refrigerator and others stored at room temp. I went through some of the Histo books and I cannot find any specifics on storage of reagents. Any suggestions?? Thanks!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Special Stain Storage
That is a new one on me. It has never come up during any of our inspections. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Monday, July 26, 2010 12:08 PM To: histonet Subject: [Histonet] Special Stain Storage Hello All, We were recently inspected by CLIA and our inspector noticed that we didn't have reagent storage temperatures written in our Special Stains procedures. We do our stains by hand so we do have some stored in the refrigerator and others stored at room temp. I went through some of the Histo books and I cannot find any specifics on storage of reagents. Any suggestions?? Thanks!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] special stain storage
The critical issue that your CLIA, or perhaps CAP, inspector is going to be asking is: is it necessary to storage stain reagent X in a refrigerator with a temperature range of Y. That answer lies in your procedure. If it says refrigerate (perhaps with a range) reagent X, then you must document that you have done so by tracking the frig temperature. If it really is not necessary to refrigerate the reagent (perhaps it is done so just to keep it from evaporating) indicate that in the procedure. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Powdered reagent expiration dates
We were inspected by CAP on friday and we were cited for ANP.21366 *Are reagents and solutions properly labeled, as applicable and appropriate, with the following elements?* * * 1. *Content and quantity, concentration or titer* 2. *Storage requirements* 3. *Date prepared or reconstituted by laboratory* 4. *Expiration date* Specifically that our staining powders didn't have an expiration date printed on the bottle. All of our reconsituted reagents which are in use were dated with an expiration date properly though. I have always assumed, perhaphs incorrectly, that powdered stains never expire. We have powders like Luxol Echt Blau, etc. that were purchased and opened over 40 years ago.If so, then these powdered reagents have gone through CAP inspections since the beginning and this inspector was the first one to find this problem. Is this one that we might protest? -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] powder stain expiration
If the manufacturer has not included an expiration date for these, i think there probably isn't one. I would call the CAP LAP folks and ask them about that. When this kind of issue arises during an inspection, it is always better to call while the inspectors are still there. You can get an answer right then and there and it will save everyone a lot of time and hassle. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Phospho-Histone 3 (SER10) antibody
I have used a phospho-H3 Ser10 antibody successfully on FFPE sections of adult mouse heart and mouse embryos. Ours is Millipore 06-570 (formerly Upstate 06-570) Lot# DAM1644557 and HIER with 10mM sodium citrate buffer, 0.05% Tween-20 pH 6.0 works great. In the past we tried a phospho H3 Ser10 antibody from Cell Signaling Technology #9706 and it works fine on Western blots, but we could never get it to work on immunostainings. This is a different antibody and company from Millipore/Upstate. Andrea Marion Graduate Student University of Illinois - Chicago amario3 [at] uic [dot] edu I have been trying without success to get this antibody to work. Is anyone doing this antibody? We have the Leica Bond Max and would like to perform it on this platform. I have tried 2 antibodies from Cell Signaling Technology without any success. Any advice would be greatly appreciated. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] powder stain expiration
We verify these on control tissues yearly and keep a record whether or not there is an expiration date. I see no reason to throw away good material and waste yet more medical dollars. Bill Blank, MD At 10:38 AM -0700 7/26/10, Tench, Bill wrote: If the manufacturer has not included an expiration date for these, i think there probably isn't one. I would call the CAP LAP folks and ask them about that. When this kind of issue arises during an inspection, it is always better to call while the inspectors are still there. You can get an answer right then and there and it will save everyone a lot of time and hassle. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Powdered reagent expiration dates
Some of our stains are very old also, about 10 years ago we wrote on a label on each stain; Opened prior to: with that days date and Stable. We were CAP inspected many times after that and we had no problems. Just as you would label your reagents you make up as Stable when there isn't an expiration time period. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Pat Laurie foreig...@gmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 07/26/2010 01:27 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Powdered reagent expiration dates We were inspected by CAP on friday and we were cited for ANP.21366 *Are reagents and solutions properly labeled, as applicable and appropriate, with the following elements?* * * 1. *Content and quantity, concentration or titer* 2. *Storage requirements* 3. *Date prepared or reconstituted by laboratory* 4. *Expiration date* Specifically that our staining powders didn't have an expiration date printed on the bottle. All of our reconsituted reagents which are in use were dated with an expiration date properly though. I have always assumed, perhaphs incorrectly, that powdered stains never expire. We have powders like Luxol Echt Blau, etc. that were purchased and opened over 40 years ago.If so, then these powdered reagents have gone through CAP inspections since the beginning and this inspector was the first one to find this problem. Is this one that we might protest? -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 80, Issue 30
Try reducing the processing time and try very fine cutting and I think the microchatter will go. Thanks Prashant Sareen BS , M.B.A , HT (ASCP) Associate Scientist III Comprehensive Animal health Services Bioreliance Corporation 14930 Broschart Road, Rockville, MD 20850 Office: 301-610-2744 Histology: 301-610-2997 Cell: 240-315-5541 Email: prashant.sar...@bioreliance.com From: histonet-requ...@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 30 To: histonet@lists.utsouthwestern.edu Date: Mon, 26 Jul 2010 10:02:53 -0700 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: Artifacts in histology section (Joseph Saby) 2. Re water problem (Steven Weston) 3. RE: Re water problem (Goins, Tresa) 4. Re: Artifacts in histology section (kim.dona...@bhcpns.org) 5. Special Stain Storage (kristen arvidson) 6. RE: Special Stain Storage (Podawiltz, Thomas) -- Message: 1 Date: Sun, 25 Jul 2010 10:49:05 -0700 (PDT) From: Joseph Saby saby_josep...@yahoo.com Subject: Re: [Histonet] Artifacts in histology section To: Aazath Raj aaz...@hotmail.com, histonet@lists.utsouthwestern.edu Message-ID: 431113.5115...@web114420.mail.gq1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 What you are describing might be microchatter. These will be sharp parallel lines/cracks that run parallel to the knife edge and are only visible under the microscope. The usuall cause is a combination of overprocessing and rough facing that is too aggressive and/or with too dull a blade. Overprocessing makes the tissue very hard and somewhat brittle. The thick sections/dull knife cause the tissue to compress and then release, causing the chatter. The actual danage is in the block face. Once you have the problem in a block, if the tissue is thick enough, you might be able to repeatedly soak the block in ice water and gently (with a fairly sharp knife) reface. With luck, you might be able to get through the damaged block face. Another artifact I have seen is similar, but the chatter appears very blurry. This is usually caused be poor fixation/processing, then oversoaking the blocks after facing. The trick here is to reface the block, then chill it without exposure to water. I've sectioned such blocks after placing them in a freezer to chill them thoroughly. This will help to obtain a section, but may not fix the staining problems that might show up later. Good luck! Joe Saby, BA HT From: Aazath Raj aaz...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Fri, July 23, 2010 11:26:28 AM Subject: [Histonet] Artifacts in histology section Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Mon, 26 Jul 2010 13:49:31 +1000 From: Steven Weston steven.wes...@utas.edu.au Subject: [Histonet] Re water problem To: histonet list histonet@lists.utsouthwestern.edu Message-ID: c87342eb.2535%steven.wes...@utas.edu.au Content-Type: text/plain; charset=iso-8859-1 We had this problem when we started using APTS coated slides and left out adhesive from the water bath. A simple solution I have found is to add a single drop of triton x100 (or similar detergent) to my full water bath and mix before heating the water. This reduces the surface tension of the water and allows it to run off the
Re: [Histonet] Powdered reagent expiration dates
Coincidentally, I had a question on this subject this morning. We rec'd in some oxalic acid and phoshotungstic acid, neither of which had expiration dates. I called Fisher and they looked it up for me and told me their expiration and offered to send it to me in writing, if I wanted. (it was 3-5 years from the manufacture date.) I was recently told by another tech that she thought 5 years was the shelf life of powders, but I wanted to see if that held true. Moral of the story is that it never hurts to call the manufacturer. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Pat Laurie foreig...@gmail.com 7/26/2010 1:27 PM We were inspected by CAP on friday and we were cited for ANP.21366 *Are reagents and solutions properly labeled, as applicable and appropriate, with the following elements?* * * 1. *Content and quantity, concentration or titer* 2. *Storage requirements* 3. *Date prepared or reconstituted by laboratory* 4. *Expiration date* Specifically that our staining powders didn't have an expiration date printed on the bottle. All of our reconsituted reagents which are in use were dated with an expiration date properly though. I have always assumed, perhaphs incorrectly, that powdered stains never expire. We have powders like Luxol Echt Blau, etc. that were purchased and opened over 40 years ago.If so, then these powdered reagents have gone through CAP inspections since the beginning and this inspector was the first one to find this problem. Is this one that we might protest? -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Special Stain Storage
Hi All, In Charles J Churukian's Manual of the Special Stains Laboratory of the Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY pg 192, he suggests the following chemicals should be kept refrigerated: all buffer solutions, hydroquinone, Phosphomolybdic acid, Phosphotungstic acid, Protargol, silver nitrate and all solutions containing silver nitrate. This information is under the section labeled Stability of Special Staining Solutions - General guidelines Concerning Solutions. Everywhere I have ever worked, we also kept the Schiff's reagent refrigerated for longer shelf life. Regards, Denise Long Woodward, MS, HT (ASCP) HTL, QIHC University of Connecticut -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Monday, July 26, 2010 12:08 PM To: histonet Subject: [Histonet] Special Stain Storage Hello All, We were recently inspected by CLIA and our inspector noticed that we didn't have reagent storage temperatures written in our Special Stains procedures.? We do our stains by hand so we do have some stored in the refrigerator and others stored at room temp.? I went through some of the Histo books and I cannot find any specifics on storage of reagents.? Any suggestions?? ? Thanks!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Myocardium infected with influenza
Does anyone have a block of myocardial tissue with a known influenza infection they can spare? Even a few slides would be great. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Powdered reagent expiration dates
See if you can get the following article. Biotech Histochem is published by the Biological Stain Commission. http://www.biologicalstaincommission.org/ Biotech Histochem. 2009 Feb;84(1):11-5. Stain and dye stability over a 30-year period: a comparison of certified dye powders by the Biological Stain Commission. Penney DP, Frank M, Fagan C, Willis C. Biological Stain Commission, Department of Pathology Laboratory Medicine, University of Rochester Medical Center, Rochester, New York 14642-0001, USA. david_pen...@urmc.rochester.edu Abstract The Biological Stain Commission (BSC) Assay Laboratory has received numerous inquiries during the past several years regarding the long-term stability of stain and dye powders, particularly since packaging requirements call for expiration dates on reagents. We have conducted a study to examine the long-term stability of selected dye powders. We used the standard procedures of the BSC for testing biological stains for certification to give an indication of the long-term chemical stability as well as staining performance of the dye powders. An earlier study by Emmel and Stotz examined the stability of various dye powders after a five-year storage period. The present study is a follow-up project covering the same dyes after storage for 30 years. The dye samples chosen for the study are the same samples used in the five-year storage period study and give comparative results for all three time periods. The results of this study affirm the generally held speculation that dye powders are stable for many years and thus have a substantial shelf-life. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Monday, July 26, 2010 1:27 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Powdered reagent expiration dates We were inspected by CAP on friday and we were cited for ANP.21366 *Are reagents and solutions properly labeled, as applicable and appropriate, with the following elements?* * * 1. *Content and quantity, concentration or titer* 2. *Storage requirements* 3. *Date prepared or reconstituted by laboratory* 4. *Expiration date* Specifically that our staining powders didn't have an expiration date printed on the bottle. All of our reconsituted reagents which are in use were dated with an expiration date properly though. I have always assumed, perhaphs incorrectly, that powdered stains never expire. We have powders like Luxol Echt Blau, etc. that were purchased and opened over 40 years ago.If so, then these powdered reagents have gone through CAP inspections since the beginning and this inspector was the first one to find this problem. Is this one that we might protest? -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
