Re: [Histonet] shrinkage
I seem to remember a good discussion in this in the following book:. L. P. Kok and M. E. Boon. *Microwave Cookbook for Microscopists*, Coulomb Press Leyden, Leiden (1992) p. 1–432 . Whetehr or not it is still available is another matter On Tue, Aug 24, 2010 at 5:17 PM, Edwards, Richard E. r...@leicester.ac.ukwrote: Anybody aware of the degree of shrinkage in paraffin processed tissues and/or GMA processed tissues?, many thanks. Cheers Richard Edwards Leicester University. Leicester U.K. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lectin From Arachis hypogaea(peanut)- peroxidase Staining
Hi Histonet, I am trying to do some staining using Lectin from Arachis hypogaea(peanut)-Peroxidase( FROM SIGMA), and wondering if anyone uses that stain if so, could you please share the protocol with me? Your help will be much appreciated! Thank you Chakib HTL From MIT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana vs Leica
Bond III hands down; I know Ventana are cutting pricing drastically to get machines in to labs, but the Bond is much more user friendly, especially if you use concentrated antibodies, and also employs the same detection kit for ISH as it does for IHC Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 24, 2010 5:10 PM To: Pamela Marcum; Maria Katleba Cc: histonet; bsulli...@shorememorial.org; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana vs Leica We recently acquired the Bond III. It was a tough decision, but space was the main reason we went with Leica. The Ventana sales team that visited our lab was very eager to get the account. Their pricing was not much different. They even offered to credit us for additional waste disposal. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Pamela Marcum Sent: Tuesday, August 24, 2010 3:48 PM To: Maria Katleba Cc: histonet; bsulli...@shorememorial.org; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica I totally agree and we have an Ultra, 2 XTs and 3 Benchmarks. They are good systems that need to get cheaper and more flexible. I am demoing the Bond III and love the ease of use, flexibility and cost savings, most. Maria is right it is about 40% less to run than a Ventana. Pam Marcum AP Manager UAMS Little Rock, AR - Original Message - From: Maria Katleba maria.katl...@stjoe.org To: bsulli...@shorememorial.org, Jay Lundgren jaylundg...@gmail.com Cc: histonet histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it..BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. Does IHC and ISH (yes- so does the Ventana) 2. Continuous feed (Ventana does NOT offer this) 3. Space saver, much smaller footprint than Benchmark 4. No wasted antibodies... 5. Not forced to buy EXPENSIVE prep kits either 6. Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. Won't blow tissue off the slide! 8. No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of bsulli...@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren jaylundg...@gmai l.com To Sent by: histonet histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm
[Histonet] shrinkage/a howlong is a piece of string type question
Many thanks to all who responded, for paraffin processed tissues the figures suggested for the amount of shrinkage found or expected were :- more than 5%:5-10%:10%(twice):10-15%:20%:25%:30-35%:30-40%, one responder felt it was noticeable and another thought it was a fairy tale concocted by pathologistsunsurprisingly many responders thought that the degree of shrinkage was dependent on the fixative used, processing schedule and the nature of the tissue itself, e.g. amount of lipid present. As far as shrinkage with GMA processed tissue go, a single response of 5% was quoted. Richard Edwards ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana vs Leica
Toni brings up a very good point you know it's pretty bad when a company is willing to pay your waste costs... Not a good business plan. Why not 'fix' the machine so that it's more green Maria Katleba MS HT(ASCP) -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Tuesday, August 24, 2010 2:10 PM To: Pamela Marcum; Maria Katleba Cc: histonet; bsulli...@shorememorial.org; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana vs Leica We recently acquired the Bond III. It was a tough decision, but space was the main reason we went with Leica. The Ventana sales team that visited our lab was very eager to get the account. Their pricing was not much different. They even offered to credit us for additional waste disposal. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Pamela Marcum Sent: Tuesday, August 24, 2010 3:48 PM To: Maria Katleba Cc: histonet; bsulli...@shorememorial.org; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica I totally agree and we have an Ultra, 2 XTs and 3 Benchmarks. They are good systems that need to get cheaper and more flexible. I am demoing the Bond III and love the ease of use, flexibility and cost savings, most. Maria is right it is about 40% less to run than a Ventana. Pam Marcum AP Manager UAMS Little Rock, AR - Original Message - From: Maria Katleba maria.katl...@stjoe.org To: bsulli...@shorememorial.org, Jay Lundgren jaylundg...@gmail.com Cc: histonet histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it..BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. Does IHC and ISH (yes- so does the Ventana) 2. Continuous feed (Ventana does NOT offer this) 3. Space saver, much smaller footprint than Benchmark 4. No wasted antibodies... 5. Not forced to buy EXPENSIVE prep kits either 6. Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. Won't blow tissue off the slide! 8. No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of bsulli...@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren jaylundg...@gmai l.com To Sent by: histonet histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches.
Re: Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question
Hey lady, How are you? I haven't seen you on Histonet much lately. I hope that means that you are doing fun things and not working so hard. We have settled in Plano. It's so nice to be around family! Will I see you at NSH? If so, we have to have our night out again so we can catch up on gossip... Kind regards, Jan Minshew Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 Office: 847.405.7051 Cell: 847.970.8468 Fax: 847.405.6560 www.leica-microsystems.com Click Here for this month's special offers! [1]http://www.leica-microsystems.com/bsdspecial gayle callis gayle.cal...@bresnan.net Sent by: histonet-boun...@lists.utsouthwestern.edu 08/25/2010 10:59 AM To 'Edwards, Richard E.' r...@leicester.ac.uk, histonet@lists.utsouthwestern.edu cc Subject Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question Have you ever thought of doing a shrinkage test? Take a tissue specimen, and xerox or use a flat bed scanner. Put fixed sample between plastic sheets, and scan it as unfixed tissue, fixed before processing and then after processing while in a faced paraffin block. Take all the measurements and then do the calculations./We used to xerox large stained bone sections, a clever way of getting a precise macro-images of a huge specimen to show gross features of a defect. This did a better job than trying to do a macro-photo with a camera or through a microscope (the latter doesn't happen). Years ago, when preparing for HTL exam practical, the samples e.g. tissue sections submitted had to be within a certain size range, and it was duly noted that after processing, the samples had shrinkage. This required going back to fixed tissue and cutting a bigger piece to compensate for the shrinkage and have a final correct sample/section size to follow the practical rules. As for GMA, there is a special processing schedule given to me that does not use alcohol dehydration (for lipid staining work). This protocol uses an GMA/watergradient since GMA is miscible with water. I would think there would be even less shrinkage with a water/GMA gradient and the source of shrinkage would come from the heat of polymerization and possibly a bit from kind of fixative used. The heat can controlled to some degree by doing polymerization on ice, or in a refrigerator, with the round JB4 metal chucks to dissipate the heat. Once again, I agree with Bryan Hewlett's assessment of shrinkage. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Edwards, Richard E. Sent: Wednesday, August 25, 2010 7:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shrinkage/a howlong is a piece of string type question Many thanks to all who responded, for paraffin processed tissues the figures suggested for the amount of shrinkage found or expected were :- more than 5%:5-10%:10%(twice):10-15%:20%:25%:30-35%:30-40%, one responder felt it was noticeable and another thought it was a fairy tale concocted by pathologistsunsurprisingly many responders thought that the degree of shrinkage was dependent on the fixative used, processing schedule and the nature of the tissue itself, e.g. amount of lipid present. As far as shrinkage with GMA processed tissue go, a single response of 5% was quoted. Richard Edwards ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database
[Histonet] Re: Testing for shrinkage
Taking simple measurements of pieces of tissue and then repeating the measurements following fixation, processing and infiltration will certainly allow for estimating the degree of macroscopic shrinkage. Unfortunately, this is not the only shrinkage that takes place, as a simple look at the finished section with a microscope will show. Cells separated from other cells, fibres split off from other fibres, and the like, are all due to microscopic shrinkage and are in addition to the macroscopic shrinkage determined by simple measurement of tissue. I have always presumed that this is why Baker's figures appear to be quite high, because he factored in all forms of shrinkage, not just the obvious. Bryan Llewellyn - Original Message - From: gayle callis gayle.cal...@bresnan.net To: 'Edwards, Richard E.' r...@leicester.ac.uk; histonet@lists.utsouthwestern.edu Sent: Wednesday, August 25, 2010 8:59 AM Subject: Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question Have you ever thought of doing a shrinkage test? Take a tissue specimen, and xerox or use a flat bed scanner. Put fixed sample between plastic sheets, and scan it as unfixed tissue, fixed before processing and then after processing while in a faced paraffin block. Take all the measurements and then do the calculations./We used to xerox large stained bone sections, a clever way of getting a precise macro-images of a huge specimen to show gross features of a defect. This did a better job than trying to do a macro-photo with a camera or through a microscope (the latter doesn't happen). Years ago, when preparing for HTL exam practical, the samples e.g. tissue sections submitted had to be within a certain size range, and it was duly noted that after processing, the samples had shrinkage. This required going back to fixed tissue and cutting a bigger piece to compensate for the shrinkage and have a final correct sample/section size to follow the practical rules. As for GMA, there is a special processing schedule given to me that does not use alcohol dehydration (for lipid staining work). This protocol uses an GMA/watergradient since GMA is miscible with water. I would think there would be even less shrinkage with a water/GMA gradient and the source of shrinkage would come from the heat of polymerization and possibly a bit from kind of fixative used. The heat can controlled to some degree by doing polymerization on ice, or in a refrigerator, with the round JB4 metal chucks to dissipate the heat. Once again, I agree with Bryan Hewlett's assessment of shrinkage. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Wednesday, August 25, 2010 7:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shrinkage/a howlong is a piece of string type question Many thanks to all who responded, for paraffin processed tissues the figures suggested for the amount of shrinkage found or expected were :- more than 5%:5-10%:10%(twice):10-15%:20%:25%:30-35%:30-40%, one responder felt it was noticeable and another thought it was a fairy tale concocted by pathologistsunsurprisingly many responders thought that the degree of shrinkage was dependent on the fixative used, processing schedule and the nature of the tissue itself, e.g. amount of lipid present. As far as shrinkage with GMA processed tissue go, a single response of 5% was quoted. Richard Edwards ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __ The message was checked by ESET Smart Security. http://www.eset.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Testing for shrinkage
Security, version of virus signature database 5396 (20100825) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __ The message was checked by ESET Smart Security. http://www.eset.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] pituitary block or microarrays
I get all my arrays from Biochain. They seem to be pretty e= asy to
deal with and their quality is hands down better than some other pla ces
I have used =) I looked for your pituitary and it said you
woul= d have to call them for availability? Good Luck!
Sarah Goebel, B.A., = HT (ASCP)
Histotechnician
= /div
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, T= exas 78744
(512)386-5107
= br
Original Message
Subject: [Histonet] pituitary block or microarrays
From: Houston, Ronald [1]ronald.hous...@nationwidechildrens.org
Date: Wed, August 25, 2010 10:09 am
To: histonet [2]histo= n...@lists.utsouthwestern.edu,
[3]ih...@googlegroups.com = [4]ih...@googlegroups.com
Can anyone help with a pituitary block (containing both adeno- and
neurohyp= ophysis) or point me in the direction of pituitary
microarrays (would need = 6 slides)?
Thanks
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
[5]www.childlab.com
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
[6]ronald.hous...@na
tionwidechildrens.org[7]mailto:ronald.hous...@nationwidechildrens.org
[8]www.NationwideChildrens.org= /a[9]http://www.Nationwide
Childrens.org
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interested.
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References
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3. 3Dmailto:ih...@googlegroups.com;
4. 3Dmailto:ih...@googlegroups.com;
5. 3Dhttp://www.childlab.com/
6. 3Dmailto:ronald.hous...@nationwidechildrens.org;
7. 3Dmailto:ronald.hous...@nationwidechi 8.
3Dhttp://www.NationwideChildrens.org/
9. 3Dhttp://www.NationwideChildrens.org/
10. 3Dmailto:Histonet@lists.utsouthwestern.edu;
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[Histonet] Re: Histonet Digest, Vol 79, Issue 38
Help!! I use a Ventana Benchmark XT. Recently I have gotten a brown granular staining of cytoplasm, not as dark as true specific staining. - Apocrine metaplasic cells. - Non-specifically staining neoplastic prostatic epithelial cytoplasm. Benign prostatic cytoplasm on prostate bx using CK 34BETA12, but not just that antibody as it also appeared in breast tissue on ER. At first I considered it was Endogenous Biotin, so I ran multiple prostate biopsy slides with a blocker but they did not improve. However a strange thing occurred on that run. On two of the slides, one section of tissue stained good, but another section of tissue on the same slide did not stain well. I hope maybe somebody has an idea or two that will help. Thanks, Chris Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email to your outgoing mail. histonet-requ...@lists.utsouthwestern.edu 6/29/2010 1:44 PM Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Canine bone sectioning trouble (Schneider,Lynda S) 2. RE: Coverslips (Malika Benatti) -- Message: 1 Date: Tue, 29 Jun 2010 12:17:58 -0400 From: Schneider,Lynda S emly...@pathology.ufl.edu Subject: [Histonet] Canine bone sectioning trouble To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 94a0bfce84cb0f4a84c50eac9b02d0a90194232...@hsc-cms01.ad.ufl.edu Content-Type: text/plain; charset=us-ascii Hello out there... We are having trouble sectioning canine bone. The samples are bone marrow cubes approximately 2cm thick and 1in wide. They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier. They were faced in and then surface decaled again for about 30 mins. When sectioned, much of the marrow was missing and the bone was torn and shredded. We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again. The samples were then reprocessed as they had been originally. The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so. This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up. If sections are even obtainable they are of horrible quality. Does anyone have any suggestions? Thank you so much in advance! Lynda -- Message: 2 Date: Tue, 29 Jun 2010 17:53:26 +0100 From: Malika Benatti ben...@gosh.nhs.uk Subject: [Histonet] RE: Coverslips To: histonet@lists.utsouthwestern.edu Message-ID: 4c2a3319.4626.003...@gosh.nhs.uk Content-Type: text/plain; charset=UTF-8 ** Proprietary ** ** Reply Requested When Convenient ** Hi there, We use the CellPath CoverSlips 22x50mm No 1.5 they are great for hand mounting but when used with the Leica CV5030 they are a real pain. most of the CoversSlips get rejected. If anyone use the same equipment, let me know how you are getting one . Cheers, Malika -- Message: 7 Date: Mon, 28 Jun 2010 15:06:08 -0400 From: Weems, Joyce jwe...@sjha.org Subject: [Histonet] RE: Coverslips To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 92ad9b20a6c38c4587a9febe3a30e164015dfd5...@chexcms10.one.ads.che.org Content-Type: text/plain; charset=us-ascii I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing.
[Histonet] pErk/Ki67 doublelabeling
I am working with fixed (10% buffered formalin) rat brain sections and I have had some difficulty getting pErk/Ki67 immunofluorescent colabeling to work. I get beautiful Ki67 labeling but absolutely no pErk labeling anywhere. I am using mouse monoclonal pErk (Cell Signaling) and rabbit monoclonal Ki67(Vector) antibodies. Primaries are incubated simultaneously for 48 hr at 4 degree C. I am using Alexa 488 and 546 secondary antibodies, which are always given simultaneously during staining for 3 hr at RT. Monolabeling with pErk is fine with other brain sections (the only difference is that they were sectioned on a freezing microtime versus a vibratome in the present study). However, the only labeling I see with pErk in my sections is nonspecific labeling of capillary beds throughout the section. I have tried staining both with and without citrate buffer antigen retrieval and there is no difference. Does anyone have any suggestions or recommendations? Neil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Volumetric determination of a lesion in a rat spinal cord
Hello, I am looking for a CRO well-versed in the volumetric determination and 3D reconstruction of demyelinated lesions located in the rat spinal cord. Hopefully, also with experience automating the measurement in luxol fast blue stained sections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: [IHCRG] PAX-5 - B-cell specific activator protein (BSAP)
We use a mouse mAb (clone 24) from BD Biosciences on Leica-Microsystems' Bond with absolutely no problems. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Taylor, Jean jtay...@meriter.com 8/19/2010 10:44 AM Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, August 18, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: CD34 positive control (McMahon, Loralee A) 2. Ventana Renaissance Containers (ricky hachy) 3. CD34 Control (Silverman, Jeffrey) -- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: McMahon, Loralee A loralee_mcma...@urmc.rochester.edu Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger jreichensper...@siumed.edu, Histonet histonet@lists.utsouthwestern.edu Message-ID: c27aa2a01cef31469813089e226f582e02d5a7c...@urmcms7.urmc-sh.rochester.edu Content-Type: text/plain; charset=us-ascii Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensper...@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensper...@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Wed, 18 Aug 2010 16:28:39 + From: ricky hachy elc...@hotmail.com Subject: [Histonet] Ventana Renaissance Containers To: Histonet histonet@lists.utsouthwestern.edu Message-ID: snt114-w54ab5f08e7e0cfc97193edcf...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Hello , I need 4 good plastic containers, where you fill with xilene,alcool... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky -- Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: Silverman, Jeffrey jsilver...@nshs.edu Subject: [Histonet] CD34 Control To: 'jreichensper...@siumed.edu' jreichensper...@siumed.edu Cc: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 83f4d81747a7094dafe3ae87151ecb941ce4144...@sykechxvs01.nslijhs.net Content-Type: text/plain; charset=us-ascii Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee
