Re: [Histonet] Re: Masson's trichrom - problem with nuclei staining
Hi, What concentration of HCL are you using in solution B ? is it 1% in the end, so i will have to use 3ml of the standard 36% HCL in 100ml, not just 1ml as mentioned in the protocols ? Itai *gayle callis** **gayle.callis @t bresnan.net *histonet%40lists.utsouthwestern.edu?Subject=%5BHistonet%5D%20Re%3A%20Poor%20Weigerts%20Hematoxylin%20staining%20with%20Massons%0A%09Trichrome%20In-Reply-To= * **Fri Sep 17 11:40:13 CDT 2010* - *Previous message: **[Histonet] Re: Masson's trichrom - problem with nuclei staining*http://lists.utsouthwestern.edu/mailman/htdig/histonet/2010-September/052630.html - *Next message: **[Histonet] RE: Cutting, Processing, etc*http://lists.utsouthwestern.edu/mailman/htdig/histonet/2010-September/052610.html - *Messages sorted by: **[ date ]*http://lists.utsouthwestern.edu/mailman/htdig/histonet/2010-September/date.html#52604 * **[ thread ]*http://lists.utsouthwestern.edu/mailman/htdig/histonet/2010-September/thread.html#52604 * **[ subject ]*http://lists.utsouthwestern.edu/mailman/htdig/histonet/2010-September/subject.html#52604 * **[ author ]*http://lists.utsouthwestern.edu/mailman/htdig/histonet/2010-September/author.html#52604 -- *In general, we found the original/classic Weigerts iron hematoxylin stain to be weak and almost washed out of tissues after staining with Massons Trichrome. We no longer use the original formula, but a more concentrated modified formulation that is not differentiated out as much by the acidifed solutions found in Mass Tri. We also found this problem with Massons Trichrome kit components, where companies probably package the original method's solutions. We make up Weigerts fresh each time, and if it will last for a week for you, fine. but our work tends to be a one time staining with Mass Tri, then weeks before it was done again. The problem is: ferric chloride continues to oxidize the hematoxylin throughout over time, that week or longer, weakening the iron hematoxylin staining capabililty. This is discussed in Sheehan and Hrapchak Theory and Practice of Histotechnology. Acid decalcified bone presents even more of a challenge, since nuclei (DNA/RNA) in cells are hydrolyzed by acid decalcifiers, compromising staining of nuclei, a problem seen with routine HE staining. Deanna was correct on her assessment of this stain for best results. This modified Weigerts Hematoxylin was published in J of Histotechnology in a paper on Kreybergs stain on skin. The second Extra Strength Weigerts was found on Histonet years ago and I have not tried the latter. I suggest you see which one you prefer, as we use the first Modified Weigerts. Over the years, the modified gave us far superior nuclear staining with Massons Trichrome. Weigert's Iron Hematoxyin MODIFIED (found in J of Histotechnology in a method for Kreybergs stain on mouse skin). Solution A. 2% Hematoxylin in 95% ethanol Solution B. 62% Ferric Chloride 4 ml Distilled water 95 ml Hydrochloric acid 1 ml Mix equal amounts of Solution A and Solution B MIX FRESH JUST BEFORE USE AND DISCARD AFTER USE. Extra Strength Weigerts Iron Hematoxylin from Histonet (reference unknown, but supposedly from J of Histotechnology and method originated by Mabel Myli, Mayo Clinic) Solution A: Hematoxylin 10 g 95% ethanol 100 ml Solution B: 11.6 g Ferric Chloride 980 ml Distilled water 10 ml 25% hydrochloric acid Working Stain Solution Solution A 5 ml Solution B 25 ml Absolute Ethanol 20 ml Staining time for both of these formulations is 10 minutes, followed by rinsing for 10 min in running tap water (hematoxylin will be black) Gayle M. Callis HTL/HT/MT(ASCP) You Wrote: I do Weigert's staining for 10 minutes and use it for a week at the most. I, too, have heard conflicting times about the stability of the Weigert's, but have the best results with using it no longer than a week. Deanna Rhoads HT (ASCP) * *2010/9/18 Andrea Marion **amar...@uic.edu** * *Hi Itai, I am thinking that we will purchase a different Weigert's from another company, or try one of the modified ones that were recommended on the listserv. Running tap water only means that you are replacing the water the slides are sitting in continuously, so that they have a constant supply of fresh water. This removes any excess stain that may be bleeding from the slide, and prevents it from recoloring the water, and restaining the tissue. Essentially it helps pull more excess stain out of the slide. Leaving it in tap water does not have the same effect, because the dye will reach an equilibrium point whereby no more will be removed from the tissue. We use a coplin
[Histonet] Cutting Microarrays
Good Morning All, I am having some issues when cutting a microarray containing 4mm punches. The punches are rolling and separating from the paraffin during cutting. Anyone have any tips or ideas?? Rae Staskiewicz MMCI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Cutting, Processing, etc
Whilst value for money and costings rule then the management will always look to save $$$s by hiring semi/unskilled personnel.the other point in this thread is that people are increasingly using the Histonet as an on-line and up to date text, which is surely not a bad thing?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: 17 September 2010 22:09 To: james leroux Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc Hi James, I would take it a step farther with the continuing ed. I think it's beyond the supervisors it gets up into lab administration (clinical lab world). I personally know of a group of great folks that work hard and run a quality service. In the last 3 years they've had a major drop off in their continuing ed. And it, of course, is tied to the budget. Unfortunately, in this case (my view) those making the money decisions are missing the value. It seems they're unwilling to make the investment. I fear that in 5 years or less (if it continues) this service will suffer. I suspect there are other folks out there in the same boat. My hat is off to everyone out there working hard in our field and to the enlightened administrators and physicians that advocate continuing ed. Have a great weekend. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of james leroux Sent: Friday, September 17, 2010 10:55 AM To: 'Nails, Felton'; histot...@imagesbyhopper.com; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc Felton, I would have to disagree with your assessment of the emails. Our field is very strong and is not in decline. Unfortunately, some supervisors around the country are relying on archaic methods and do not want to see or welcome change within the histo lab. We call ourselves professionals and yet not all of us are required to do continuing education? I read emails everyday and laugh at some of the bloviating that goes on inside this forum. I am glad the questions are asked, but I am also amazed at some of the responses that are shared with everyone. I choose to respond one on one with the person asking the question. Basic histology deals with didactics and this particular inquiry dealt more with OJT. There are many ways to get the same job done; are there more efficient ways? Probably, but this does not mean we all do our job the same way. I am not concerned about the future of Histotechnology. I embrace the opportunity to teach the young technicians about a field that sees a change almost daily. I am not here to offend either, but rather to defend an occupation that is as fascinating as it is frustrating. Respectfully, James Leroux, AAS, BA, HTL Histology Supervisor Petroglyph Pathology 640 Quantum Rd. Rio Rancho, NM 87124 (505) 924-0219 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 11:03 AM To: 'histot...@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the what is happening to our field, was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one
Re: [Histonet] Cutting Microarrays
hi, this sounds like the tissue was too cool when embedded in the wax - so it has not made a nice homogenous block that cuts as one. Can u re-embed? On Mon, Sep 20, 2010 at 10:54 AM, Rae Staskiewicz raest...@grics.netwrote: Good Morning All, I am having some issues when cutting a microarray containing 4mm punches. The punches are rolling and separating from the paraffin during cutting. Anyone have any tips or ideas?? Rae Staskiewicz MMCI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cutting Microarrays
Hello, We have trouble with the larger size punches as well. In order to get the cores to stick to the size try warming the block in a oven at 37 degrees for 15 minutes, put the block face down on a clean slide. (providing the melting temperature of the paraffin is about 57 degrees). After 15 minutes remove the block/slide complex and gently press down on the block, then allow the block to cool. Repeat the process 3 or 4 times. We have also had some success by sliding the block face over the heating unit of the paraffin embedder. But this only works for a few sections and then you get the same problem back. Best Regards, Helen L. Fedor Tissue Microarray Lab, Manager Prostate Tissue Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, September 20, 2010 5:51 AM To: Rae Staskiewicz; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cutting Microarrays hi, this sounds like the tissue was too cool when embedded in the wax - so it has not made a nice homogenous block that cuts as one. Can u re-embed? On Mon, Sep 20, 2010 at 10:54 AM, Rae Staskiewicz raest...@grics.netwrote: Good Morning All, I am having some issues when cutting a microarray containing 4mm punches. The punches are rolling and separating from the paraffin during cutting. Anyone have any tips or ideas?? Rae Staskiewicz MMCI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cutting Microarrays
Hello, We have trouble with the larger size punches as well. In order to get the cores to stick to the size try warming the block in a oven at 37 degrees for 15 minutes, put the block face down on a clean slide. (providing the melting temperature of the paraffin is about 57 degrees). After 15 minutes remove the block/slide complex and gently press down on the block, then allow the block to cool. Repeat the process 3 or 4 times. We have also had some success by sliding the block face over the heating unit of the paraffin embedder. But this only works for a few sections and then you get the same problem back. Best Regards, Helen L. Fedor Tissue Microarray Lab, Manager Prostate Tissue Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, September 20, 2010 5:51 AM To: Rae Staskiewicz; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cutting Microarrays hi, this sounds like the tissue was too cool when embedded in the wax - so it has not made a nice homogenous block that cuts as one. Can u re-embed? On Mon, Sep 20, 2010 at 10:54 AM, Rae Staskiewicz raest...@grics.netwrote: Good Morning All, I am having some issues when cutting a microarray containing 4mm punches. The punches are rolling and separating from the paraffin during cutting. Anyone have any tips or ideas?? Rae Staskiewicz MMCI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Masson's trichrom - problem with nuclei staining
We use Weigert's for 7 minutes and never more than a week either. As a precaution we keep it in the dark in a non-transparent container. That's the way I was always taught (26 years and counting) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deanna Rhoads Sent: Friday, September 17, 2010 10:08 AM To: Andrea Marion; histonet@lists.utsouthwestern.edu Cc: itai.mo...@mail.huji.ac.il Subject: Re: [Histonet] Re: Masson's trichrom - problem with nuclei staining I do Weigert's staining for 10 minutes and use it for a week at the most. I, too, have heard conflicting times about the stability of the Weigert's, but have the best results with using it no longer than a week. Deanna Rhoads HT (ASCP) From: Andrea Marion amar...@uic.edu To: histonet@lists.utsouthwestern.edu Cc: itai.mo...@mail.huji.ac.il Sent: Fri, September 17, 2010 10:31:16 AM Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining Hi Itai, I am interested to hear if you've resolved this problem. We use the same kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is similar to the one you mentioned. I cannot get nuclei staining with this method either. The nuclei are well stained (ie black) up to the PMA/PTA step, but during that step the nuclear stain is completely removed. I cannot shorten the PMA/PTA step without negatively effecting the collagen stain. I have in our original protocol that the Weigert's working solution is good for one month, but I cannot recall if this is from Sigma's specification sheet or a personal observation from a lab member. However, from reading online some say it is good up to 4 months, others say it needs to be prepared fresh each time. I have tried fresh preparations with the same results. My instinct is that something is off - the staining is just not stable enough to withstand the subsequent acid steps in Masson's trichrome. Can an expert weigh in on this? Is there a way to strengthen nuclear staining from Weigert's? Sigma's formulas and usage recommendations are: Part A: 1% w/v certified Hematoxylin in ethanol Part B: 1.2% w/v Ferric chloride, 1% v/v Hydrochloric Acid Combine equal volumes Part A and Part B, stain sections for 5 minutes. Andrea Marion Graduate Student University of Illinois at Chicago amario3 /at/ uic /dot/ edu Itai Moshe itai.moshe @t mail.huji.ac.il Wed Sep 15 11:34:51 CDT 2010 Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997With sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that im not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai P.S By mistake I've post this before in another message with a wrong title. please respond to that message, so the title will be o.k for future archive searching. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Posting comments
Agreed. --On Saturday, September 18, 2010 1:25 PM -0400 Carrie Disbrow cdisb...@msn.com wrote: As a newbie trying to learn as much as possible, I'm disappointed when questions are posted and the responses are sent to the individual. I learn more when the responses are shared. Also, an archive search on the subject will have limited information. I would think there are many in the same boat - those who want to learn more about the craft. Even a review of the basics is good reading for me. That is why I reply all instead of reply. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How long ...
First off you shouldn't leave Bouin's sitting for that long out o= n a
shelf. Once the water evaporates out of the solution it is extrem ely
flammable (explosive even I think?). Also, you shouldn't leave
ti= ssue in alcohol forever either because you will end up with beef
jerky su= per hard tissues. If you want to keep it in alcohol, maybe
make up an= alcoholic formalin solution. I would say try to process
it if it is = needed (it might be ok?), but if it was me I would
probably just chalk it u= p to the tissue is trash now...Good
Luck...let me know what happens!!
Sarah= Goebel, B.A., HT (ASCP)
Histotechnician
XBio= tech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100= /em
Austin, Texas 78744
= span style=color: rgb(51, 102, 255);(512)386-5107
= /div
Original Message
Subject: [Histonet] How long ...
From: Massimo [1]max_histo...@yah= oo.it
Date: Sun, September 19, 2010 3:41 pm
To: [2]histo...@lists.uts= outhwestern.edu
Cc: [3]histo...@lists.uts= outhwestern.edu
Hi all,
I found into a shelf of the laboratory a small flask containing mouse
sampl= es
fixed in Bouin's fluid and preserved in ethanol at 70°, forgotten
ther= e for a
few years.
I wonder if it would be possible to continue the process up to
paraffin
embedding for histological preparations.
Some time ago a professor of biology at the University of Florence
told me = that
they could remain in alcohol for years, but on literature I found that
the = time
can not be so long without altering tissues.
Has anyone had a similar experience?
It's better to throw everything away or not?
Thank you in advance.
Kind Regards,
Massimo Tosi
___
Histonet mailing list
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References
1. 3Dmailto:max_histo...@yahoo.it;
2. 3Dmailto:histonet@lists.utsouthwestern.edu;
3. 3Dmailto:histonet@lists.utsouthwestern.edu;
4. 3Dmailto:Histonet@lists.utsouthwestern.edu;
5. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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[Histonet] Job Opening
Hi All, Our private derm-path lab, located in central-western New York State, has an immediate opening for a full time histotech to work days. A relocation bonus is negotiable. To learn more please contact me directly. Joyce ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 82, Issue 26
We have three CBG recyclers - two are used for formalin and one is used for alcohol/xylene. They run every day and we have very little trouble with them. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA -- Message: 6 Date: Sat, 18 Sep 2010 20:45:52 -0700 From: Melinda Sokol m.soko...@gmail.com Subject: [Histonet] CBG Recycler To: histonet@lists.utsouthwestern.edu Message-ID: aanlktingwz6s1dzsqmjbjeajgncuewpvj60q3cwkv...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi All I would like Pros and Cons regarding a CBG Recycler. Please- No Vendors. Thanks Melinda A Hamilton HT (ASCP) -- NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Posting comments
-Original Message- From: Dawson, Glen Sent: Monday, September 20, 2010 9:10 AM To: 'Merced M Leiker' Subject:RE: [Histonet] Posting comments Carrie, Until you've had some psychopath threaten you with a lawsuit, or threaten to tell on you with your current employer, it will be difficult to understand why most of us choose to reply directly to whomever posts the question. I've had this happen to me on this list because someone out their did not agree with my general post needed to act like a child to get his point across. I've responded directly to the person that posts the question ever since...basically to avoid the hassle. I realize that this isn't the best situation for the distribution of useful information, but all of us need to remember that there will always be a small percentage of bad apples in the basket that is the histonet and that those of us that won't hit reply to all don't do so of our own accord. Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Monday, September 20, 2010 8:45 AM To: Carrie Disbrow; histonet@lists.utsouthwestern.edu Subject:Re: [Histonet] Posting comments Agreed. * On Saturday, September 18, 2010 1:25 PM -0400 Carrie Disbrow cdisb...@msn.com wrote: As a newbie trying to learn as much as possible, I'm disappointed when questions are posted and the responses are sent to the individual. I learn more when the responses are shared. Also, an archive search on the subject will have limited information. I would think there are many in the same boat - those who want to learn more about the craft. Even a review of the basics is good reading for me. That is why I reply all instead of reply. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Out of Office Reply
I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Questions
I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with oh no or oh my gosh - I feel as though I should apologize for my stupid question. However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
James, Well said. I totally agree Thank you very much! Amy Message: 4 Date: Fri, 17 Sep 2010 11:54:42 -0600 From: james leroux jler...@petroglyphpath.com Subject: RE: [Histonet] RE: Cutting, Processing, etc To: 'Nails, Felton' flna...@texaschildrens.org, histot...@imagesbyhopper.com, 'mohamed abd el razik' k8...@yahoo.com, Histonet@lists.utsouthwestern.edu Message-ID: 201009171758.o8hhw7cr085...@ame7.swcp.com Content-Type: text/plain; charset=iso-8859-1 Felton, I would have to disagree with your assessment of the emails. Our field is very strong and is not in decline. Unfortunately, some supervisors around the country are relying on archaic methods and do not want to see or welcome change within the histo lab. We call ourselves professionals and yet not all of us are required to do continuing education? I read emails everyday and laugh at some of the bloviating that goes on inside this forum. I am glad the questions are asked, but I am also amazed at some of the responses that are shared with everyone. I choose to respond one on one with the person asking the question. Basic histology deals with didactics and this particular inquiry dealt more with OJT. There are many ways to get the same job done; are there more efficient ways? Probably, but this does not mean we all do our job the same way. I am not concerned about the future of Histotechnology. I embrace the opportunity to teach the young technicians about a field that sees a change almost daily. I am not here to offend either, but rather to defend an occupation that is as fascinating as it is frustrating. Respectfully, James Leroux, AAS, BA, HTL Histology Supervisor Petroglyph Pathology 640 Quantum Rd. Rio Rancho, NM 87124 (505) 924-0219 Joyce, As always, your 2 cents is 'right on'! Thank you! Amy Message: 2 Date: Fri, 17 Sep 2010 13:07:16 -0400 From: Weems, Joyce jwe...@sjha.org Subject: RE: [Histonet] RE: Cutting, Processing, etc To: Nails, Felton flna...@texaschildrens.org, 'histot...@imagesbyhopper.com' histot...@imagesbyhopper.com, 'mohamed abd el razik' k8...@yahoo.com, Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 92ad9b20a6c38c4587a9febe3a30e16403984c0...@chexcms10.one.ads.che.org Content-Type: text/plain; charset=iso-8859-1 My 2 cents is that she needed to convince someone this was how it is done! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Questions
Amy, You do not need to apologize for asking a question to which you did not know the answer. This is an educational avenue, for histology, and there is no such thing as a stupid question if you need answers to solve a problem. Those of us who teach know questions are important, even if you think you know the answer but not exactly sure, or in your case you knew but needed documented verification from others in the field. I hope your fellow workers and supervisors got the message and please feel free to ask. There are those in the field who feel this is a social network for experts and that is okay too, but the real reason NSH and histosearch was started was to expand knowledge of the histology community and to improve our profession. Remembering when histology was in the basement and no one knew we were there, it makes me proud of the progress we have made in the 48 years I have been in the field. Keep asking and share what you know, no need for apologies. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Monday, September 20, 2010 11:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Questions I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with oh no or oh my gosh - I feel as though I should apologize for my stupid question. However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Questions
Agreed! Thanks, Timothy Higgins, HT(ASCP) QIHC Histology Manager APA Amarillo, TX -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, September 20, 2010 10:33 AM To: Senn, Amy R; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Questions Amy, You do not need to apologize for asking a question to which you did not know the answer. This is an educational avenue, for histology, and there is no such thing as a stupid question if you need answers to solve a problem. Those of us who teach know questions are important, even if you think you know the answer but not exactly sure, or in your case you knew but needed documented verification from others in the field. I hope your fellow workers and supervisors got the message and please feel free to ask. There are those in the field who feel this is a social network for experts and that is okay too, but the real reason NSH and histosearch was started was to expand knowledge of the histology community and to improve our profession. Remembering when histology was in the basement and no one knew we were there, it makes me proud of the progress we have made in the 48 years I have been in the field. Keep asking and share what you know, no need for apologies. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Monday, September 20, 2010 11:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Questions I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with oh no or oh my gosh - I feel as though I should apologize for my stupid question. However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Questions
Shirley and Amy and others responding to this thread: thanks for your posts. I am not in the histotechnology field - I'm actually a graduate student at Loyola who does her own processing, cutting, and staining of bone tissue - so I really appreciate every so-called dumb question, no matter how simple, since I have never been formally trained in the field. I assumed this was a great forum to safely ask those kinds of questions to advance my graduate student research. I'm learning new details about histology every day just by reading posts by helpful people like you. Thanks have a great week, Kristen Shirley A. Powell powell...@mercer.edu 09/20/10 10:35 AM Amy, You do not need to apologize for asking a question to which you did not know the answer. This is an educational avenue, for histology, and there is no such thing as a stupid question if you need answers to solve a problem. Those of us who teach know questions are important, even if you think you know the answer but not exactly sure, or in your case you knew but needed documented verification from others in the field. I hope your fellow workers and supervisors got the message and please feel free to ask. There are those in the field who feel this is a social network for experts and that is okay too, but the real reason NSH and histosearch was started was to expand knowledge of the histology community and to improve our profession. Remembering when histology was in the basement and no one knew we were there, it makes me proud of the progress we have made in the 48 years I have been in the field. Keep asking and share what you know, no need for apologies. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Monday, September 20, 2010 11:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Questions I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with oh no or oh my gosh - I feel as though I should apologize for my stupid question. However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Questions
Well said, Shirley! Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Shirley A. Powell powell...@mercer.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 09/20/2010 11:37 AM To Senn, Amy R ars...@hsh.org, Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu cc Subject [Histonet] RE: Questions Amy, You do not need to apologize for asking a question to which you did not know the answer. This is an educational avenue, for histology, and there is no such thing as a stupid question if you need answers to solve a problem. Those of us who teach know questions are important, even if you think you know the answer but not exactly sure, or in your case you knew but needed documented verification from others in the field. I hope your fellow workers and supervisors got the message and please feel free to ask. There are those in the field who feel this is a social network for experts and that is okay too, but the real reason NSH and histosearch was started was to expand knowledge of the histology community and to improve our profession. Remembering when histology was in the basement and no one knew we were there, it makes me proud of the progress we have made in the 48 years I have been in the field. Keep asking and share what you know, no need for apologies. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Monday, September 20, 2010 11:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Questions I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with oh no or oh my gosh - I feel as though I should apologize for my stupid question. However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Skills testing for a histo lab assistant
We are going to be posting a histology lab assistant position soo= n for the first time. I would like to give them a skills assessment t est to see how well they can handle a computer, numbers, problem solving, a= nd what their handwriting looks like. Does anyone out there do this f= or their new hires? What and how do you do to test? nb= sp; Cathy Crumpton HT(ASCP), Histology Lead Tuality Community = Hospital Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] multi-timer
Hi everybody! Does anyone know where I can purchase a multi-timer (product used to be manufactured by Beckman Coulter electronics)? The company no longer manufactures them. Thank you so much! Paula Wilder St. Joseph Medical Center 410-337-1741 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] multi-timer
Try Labsco. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Wilder Sent: Monday, September 20, 2010 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] multi-timer Hi everybody! Does anyone know where I can purchase a multi-timer (product used to be manufactured by Beckman Coulter electronics)? The company no longer manufactures them. Thank you so much! Paula Wilder St. Joseph Medical Center 410-337-1741 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Steedman polyester wax
I would like to know if anyone has a good protocol that includes tissue fixation (preferred fixative, fixation temperature etc) and tissue processing (time etc.) for low melting Steedman polyester wax . The tissue to be embedded is chick embryo brain. I am going to do all the tissue processing and embedding manually. Any suggestion? Looking forward to receive your advice Luisa Luisa Onstead-Cardinale, M.S. Research Associate UF Biomedical Research Laboratory, Room 121 655 West 11th Street Jacksonville, Fl 32206 phone (904) 633-0984 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] multi-timer
Hey guys Was wondering if any of you ever used a phopho NFKB p65 antibodu and which one you prefer with what antigen retrieval method if applies. I'm working on FFPE rat tissue Thanks. Fabrice ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
