[Histonet] RE: Question about acid phosphatase staining on FFPE and control tissue

2010-12-06 Thread Thurby, Christina
Hi,
We are going to try acid phosphatase staining on FFPE cardiac tissue (I have a 
couple of papers where this has been performed with success).  My question for 
any of you with expertise in this area is what type of tissue should I use as a 
Method Development control?  Would there be acid phosphatase activity in 
virtually any normal muscle tissue (ie the epitheloid cell lineage) or possibly 
just choose prostate?  Just curious if any of you out there may have a good 
suggestion for the control tissue.
Thanks,
Kristie

Christina Thurby
Bristol-Myers Squibb Company
Research Scientist I
812-307-2093 (tie 625)



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[Histonet] Dermatology Billing

2010-12-06 Thread Alyssa Peterson
Hi Histoland,

My apologies if this is a little off subject, but since it has to do with
Dermatology I thought I would give it a shot. A large billing company in
Casselberry, FL (just outside of Orlando, FL) is looking for a Manager for
National Billing Service. I wanted to do them a favor and see if thier are
any qualified candidates for this in Histoland. Please feel free to contact
me at anytime and pass this along to anyone you might know who is
interested. My contact information is below :-)


*Manager for National Billing Service (Dermatology)*

*Location:Casselberry, Florida (Orlando)*

*Employment Type:Full-time*

*Salary Range: Commensurate with experience*

*Benefits: Health, vision, dental, ST/LT
Disability, 401K, Vacation*

· Management experience with billing service or very large medical
practice required

· BS degree or five+ years of billing service-related experience
required

· Dermatology experience is a plus

· Solid leadership skills with emphasis on team-building and
performance accountability

· Strong organizational skills

· Strong financial background

· Customer service oriented geared towards quality and excellence

· Poised for company growth

· Responsibilities will include

oManaging approximately 40+ employees

oCorrespondence and conversations with physicians and medical office
staff

oMonthly presentations to internal management

§ Analyze financial status and trends

§ Analyze billing service statistical data

§ Audit and evaluate for quality and performance

oShort and long-term goal administration

oMonthly presentations to clients

§ Analyze financial status and trends

§ Analyze practice statistical data

§ Summarize practice issues, concerns and problem resolution

§ Address service quality and employee performance

§ Maintain positive and open lines of communication

oWeekly meetings with department supervisors

§ Accountability for work

§ Problem troubleshooting

§ Organizational compliance and overview

§ Focus on efficiency

oTravel required



Responsible to CEO and Owner



Please let me know if you or anyone you know may be interested. They can
contact me with their resume at aly...@alliedsearchpartners.com


-- 

* *

**If you wish to no longer receive emails from Allied Search Partners please
reply with “Remove.” *

Alyssa Peterson, Director of Candidate Recruitment
LinkedIN:http://www.linkedin.com/in/alyssapetersonasp

Allied Search Partners

T: 888.388.7571

F: 888.388.7572

www.alliedsearchpartners.com

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RE: [Histonet] Special Stain Automation

2010-12-06 Thread Bartlett, Jeanine (CDC/OID/NCEZID)
We get great silvers with our Dako Artisan.

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara
Richmond
Sent: Wednesday, December 01, 2010 10:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special Stain Automation

We are in the market for a new stainer to do special stains.  We
currently are using the Nexus and have been unhappy with it,
particularly with the silver stains for which we do a lot of.  I'm
wondering what techs out there recommend for us to check into.



-
Avera is a health ministry rooted in the Gospel. Our mission is to make
a positive impact in the lives and health of persons and communities by
providing quality services guided by Christian values. Avera is
sponsored by the Benedictine and Presentation Sisters.
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[Histonet] Workload Recording

2010-12-06 Thread Scott, Allison D
Hello to all in histoland.  Does anyone keep a log of how many blocks
that their techs cut.  My boss asked me do I keep this type of
information. Anyone doing workload recording?

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
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representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

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Re: [Histonet] High temp/paraffin oven

2010-12-06 Thread Rene J Buesa
That is a thermostat problem. Had it check out.
René J.


--- On Wed, 12/1/10, histopa...@hotmail.com histopa...@hotmail.com wrote:


From: histopa...@hotmail.com histopa...@hotmail.com
Subject: [Histonet] High temp/paraffin oven
To: Histonet histonet@lists.utsouthwestern.edu
Date: Wednesday, December 1, 2010, 5:19 PM


Hello to all,

Any idea why my middle paraffin oven would be out of range, but the othet two 
on opposite sides are not?

Histopathy
Sent on the Sprint® Now Network from my BlackBerry®
-Inline Attachment Follows-


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[Histonet] Question about acid phosphatase staining on FFPE and control tissue

2010-12-06 Thread Thurby, Christina
Hi,
We are going to try acid phosphatase staining on FFPE cardiac tissue (I have a 
couple of papers where this has been performed with success).  My question for 
any of you with expertise in this area is what type of tissue should I use as a 
Method Development control?  Would there be acid phosphatase activity in 
virtually any normal muscle tissue (ie the epitheloid cell lineage) or possibly 
just choose prostate?  Just curious if any of you out there may have a good 
suggestion for the control tissue.
Thanks,
Kristie

Christina Thurby
Bristol-Myers Squibb Company
Research Scientist I
812-307-2093 (tie 625)



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delete the message and any attachments. Any disclosure, reproduction, 
distribution or other use of this message or any attachments by an individual 
or entity other than the intended recipient is prohibited.
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[Histonet] Manual Copper Stain

2010-12-06 Thread Pamela Gholston

Does anyone have a manual copper stain procedure to share?

Thank you,Histopathy

  
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[Histonet] immersion fixation and perfusion fixation

2010-12-06 Thread ANJUM PARKAR
Hi all,
Does anyone have a protocol for whole rat brain immersion fixation using 4%
Paraformaldehyde for IHC where no transcardial perfusion has been performed? I
am specifically interested in knowing how long does the brain need to be
immersed for a good complete fixation before transferring to sucrose. Also, I
would like a protocol for rat brain tissue transcardial perfusion fixation down
to freezing to sectioning if possible to compare with my current protocol.
Thanks!



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[Histonet] Lab Manager Position

2010-12-06 Thread K.C. Carpenter


Dear Histonet Community,

 

I am a one of the founders of a healthcare recruiting firm.  I help Lab 
Professionals find permanent employment and I wanted to see if you are 
interested in exploring other career opportunities?  We are completely free of 
charge to candidates and and we work on laboratory openings across the country. 
Our clients typically assist with relocation expenses. I am currently working 
on some great positions that you may be interested in including a Laboratory 
Manager opening at a Dermpath Lab.

 






My client is a Pre-IPO Pathology company who is quickly establishing itself as 
an industry leader. I'm contacting you to see if you'd be interested in 
pursuing the Lab Manager position they have open in their Dermpath Lab in Long 
Island, NY. This state of the art lab s looking for someone with prior 
management experience to run and manage a lab consisting of 10 FTE's. This 
Manager will be responsible for overseeing the day to day operations of the 
lab.   My clients offers one of the best compensation  benefits package around 
including relocation assistance when necessary.   For consideration you must 
have a Bachelor's Degree, be HT or HTL (ASCP) certified and licensed in the 
State of NY.  A minimum of 2 years of prior supervisory or management 
experience is preferred.  If you are interested in learning more about this 
position, please call or email me at k...@ka-recruiting.com

 

Please contact me if you're interested in learning more about this position or 
in exploring some of the other numerous job openings we have available across 
the country. 

 

Have a great weekend. 















KC Carpenter



K.A. Recruiting, Inc.


10 Post Office Square, 8th Floor South


Boston, MA 02109


P: (617) 692-2949


F: (617) 507-8009



k...@ka-recruiting.com



www.ka-recruiting.com
  








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[Histonet] Competencies

2010-12-06 Thread Paula Wilder

When a Histotech leaves their place of employment - does anyone know if their 
competencies need to be kept for any period of time or can the competencies be 
disgarded after their departure?  Thanks for your help!
 
Paula Wilder
St. Joseph Medical Center 
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[Histonet] Contact information

2010-12-06 Thread Rae Staskiewicz
Could anyone send me the contact information for Charlie Doerner?

 

Rae Ann Staskiewicz

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[Histonet] FW: TBS Histology Equipment

2010-12-06 Thread Damaris Beil



 
 
 
 
 
Damaris E. Beil, HT,ASCPcm, HTL
Associates in Dermatology
Histology Laboratory
(407) 846-7546 ext. 3307



 


From: dbe...@hotmail.com
To: histonet@lists.utsouthwestern.edu
Subject: TBS Histology Equipment
Date: Fri, 3 Dec 2010 10:48:04 -0500




Hello everyone,
 
I was wondering if any of you would be able to give me feedback on the TBS ATP 
processor, TEC embedding center, SHUR/Stain HE stainer, microtomes and other 
piece of  instrument from TBS/SHUR.
 
Thank You,
 
 
 
 
 
Damaris E. Beil, HT, ASCPcm,HTL 

Associates in Dermatology
Histology Laboratory
(407) 846-7546 ext. 3307 
 
 
 

 

  
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[Histonet] Protocol for immunofluorescence staining

2010-12-06 Thread tahseen
Dear All,
We are looking protocol for immunofluorescence staining Fibrinogen/FITC
and positive control, negative control tissue.
Muhammad Tahseen
SKMCHRC
Lahore,Pakistan



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[Histonet] Eosin in Tissue Processor

2010-12-06 Thread Akemi Allison

Happy Friday Everyone,

I am sending this inquiry again.  For some reason this post did not  
show I my histonet emaiI.  I guess I am having a old persons  
momment!  I forgot how much eosin and what % to put in the last 100%  
of the Tissue Processor to show-up small transparent bx's.  Can one  
of you wonderful people tell me please.  The tissue processor is a  
VIP 5.


Thanks a bunch!


Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3...@yahoo.com

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[Histonet] New Histology Field Technical Role Based in AZ/TX/Southern CA

2010-12-06 Thread Matt Ward
Good Afternoon Everyone!



We have had a world leader open a new Field Technical Histology position
that will cover the Pacific Region. The best place to live for this position
would be Phoenix, Los Angeles, Or Dallas, and it will focus on Core
Histology. This is perfect for a Histotech that is looking for an
opportunity to get out of the lab in into the field with a growing company
that offers promotion.



Please contact me directly by sending me a copy of your resume to
m...@personifysearch.com.



Thanks!



Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

 www.personifysearch.com
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[Histonet] Hemoglobin A

2010-12-06 Thread Joe Nocito
Howdy histoland,
does anyone know where to get Hemoglobin A? Dako doesn't have it any more and 
we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc, 
Biogenex. No luck. Thanks

Joe
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[Histonet] Problems with histonet

2010-12-06 Thread Akemi Allison
I just made 2 posts today and they are not showing up.  Is any one having 
problems too?
 Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com


  
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[Histonet] TBS Histology Equipment

2010-12-06 Thread Damaris Beil

Hello everyone,
 
I was wondering if any of you would be able to give me feedback on the TBS ATP 
processor, TEC embedding center, SHUR/Stain HE stainer, microtomes and other 
piece of  instrument from TBS/SHUR.
 
Thank You,
 
 
 
 
 
Damaris E. Beil, HT, ASCPcm,HTL 
Associates in Dermatology
Histology Laboratory
(407) 846-7546 ext. 3307
 
 
 
 

 
  
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[Histonet] Changing solutions on VIP processors.

2010-12-06 Thread Angela Bitting
Hello All,
I'm involved in a debate with a colleague about how frequently the tissue 
processors need to be changed.
We do roughly 1000 blocks a day on various runs, utilizing 7 tissue processors.
About 100 of those are microwave processed, so I'm subtracting those from the 
equation.
 
So 900 blocks (mixed tissues, no breast) divided by 6 conventional processors 
is 150 blocks each.
 
I think it's poor reagent management to change a VIP after 150 blocks. Does 
anyone disagree with me?
If these were processing very fatty tissue, I might think differently, but 
that's not the case.
 
All opinions are welcome.
Thanks,
Angie
 
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!


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[Histonet] HT Position TN

2010-12-06 Thread Hale, Meredith
 

Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mh...@carisls.com

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mh...@carisls.com mailto:mh...@carisls.com  

 

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[Histonet] Light Green

2010-12-06 Thread Breeden, Sara
Why does my light green counter stain just stain the edges of the
sections???  

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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[Histonet] TBS laboratory equipment

2010-12-06 Thread Damaris Beil

Hello everyone,
 
I 'd like to know if anyone is using  the TBS the ATP processor, Shur/Stain HE 
stainer, and TEC embedding center, manual and automated microtomes.  Any 
feedback would be greatly appreciated.
 
Thanks in advance,
 
 
 
 
 
 
 
Damaris E. Beil, HT, ASCPcm,HTL 
Associates in Dermatology
Histology Laboratory
(407) 846-7546 ext. 3307
 
 
 

 
 
  
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[Histonet] Eosin in Tissue Processor

2010-12-06 Thread Akemi Allison
Happy Friday Everyone,

I guess I am having a old persons momment!  I forgot how much eosin and what % 
to put in the last 100% of the Tissue Processor to show-up small transparent 
bx's.  Can one of you wonderful people tell me please.  I tissue processor is a 
VIP 5.

Thanks a bunch!
Akemi Allison BS, HT(ASCP)HTL
Director 
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com 



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[Histonet] Test - please disregard

2010-12-06 Thread histotech
This is a test to see if my email is working properly.  Please disregard.

Thanks!


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[Histonet] RELIA Special Job Alert Histology opportunities in NY

2010-12-06 Thread Pam Barker
Hi Histonetters!!

I hope you had a great Thanksgiving.  I have several clients that are in
need of histo techs and supervisors in New York so I thought I would put the
word out.  At this point since it is the end of the year my clients are
willing to let you call the shots on how you would like to proceed i.e.
interview now or after the holidays.  Of course these are all permanent full
time positions offering excellent salaries, benefits and relocation
assistance. 

* State of NY license or elig.*  

Here is a list of my current histo tech and supervisory positions:

Orange/Rockland County NY - Manager of QA-Private Pathology Lab

Rochester, NY - Histotech with Dermpath experience

Orange/Rockland County, NY - Day shift Histotechs

If you or anyone you know is interested call me toll free at 866-607-3542 or
e-mail me at  mailto:rel...@earthlink.net rel...@earthlink.net  

Thanks and Have a Great Day!!

 


Thank You! 

 

Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail:  mailto:rel...@earthlink.net rel...@earthlink.net 
 http://www.facebook.com/ www.facebook.com  search Pam Barker RELIA
 http://www.linkedin.com/reliasolutions www.linkedin.com/reliasolutions
 http://www.myspace.com/pamatrelia www.myspace.com/pamatrelia
 http://www.twitter.com/pamatrelia www.twitter.com/pamatrelia

 
Thank You!
  
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail:  mailto:rel...@earthlink.net rel...@earthlink.net 
 http://www.facebook.com/ www.facebook.com  search Pam Barker RELIA
 http://www.linkedin.com/reliasolutions www.linkedin.com/reliasolutions
 http://www.myspace.com/pamatrelia www.myspace.com/pamatrelia
 http://www.twitter.com/pamatrelia www.twitter.com/pamatrelia 

 
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[Histonet] Plastic discoloration

2010-12-06 Thread Herrick, James L. (Jim)
Good morning everyone,

I just have a quick question in regards to GMA turning white following
embedding. Does anyone have any experience on why our plastic is losing
its clarity and turning a whitish color? This doesn't seem to have any
impact on staining but it would be nice to know the reason. Our
polymerization is carried out in a vacuum chamber (nitrogen is used) and
BPO is used as the initiator and JB4 as the accelerator. If any on you
have any answers, your help would be greatly appreciated.
Have a great day!!
Jim




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[Histonet] Great Histology Supervisor Job in Ohio

2010-12-06 Thread Alisha Dynan


Hi Histonet Members,

 

I am currently working on a Histology Supervisor position in Ohio. The ideal 
candidates must have strong supervisory/management experience, be HT ot 
HTL(ASCP certified, and have strong experience in all areas of the histology 
lab. My client is willing to assist with relocation expenses if necessary and 
offers top notch salary and benefits.

 

If interested in learning more, please email me your resume to 
ali...@ka-recruiting.com. I will then be in touch with you to discuss this 
opportunity. Thanks!



Sincerely,

 

Alisha (Taylor) Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

ali...@ka-recruiting.com

www.ka-recruiting.com

 






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[Histonet] RELIA Histology Job Alert 12/06/10 Histotech needed in Coastal Texas - Private Pathology Lab ASCP or eligible. Can you help?

2010-12-06 Thread Pam Barker
Hi Histonetters!!
I hope everybody is having a great day.  I am working with a client on a
new position that I wanted to tell you all about.
My client is a private pathology lab located in Coastal Texas.  This is
a dayshift (early morning) full time permanent position.  It is M-F with
occasional Saturdays.  My client offers a competitive pay, benefits and
relocation assistance.  
Requirements:
ASCP or elig.
1-2 yrs plus Routine Histology experience (including hand staining)
IHC is a plus.
This is an immediate opening but my client is willing to consider
candidates who prefer to start work after the holidays as well.  If you
or anyone you know might be interested please contact me - Pam Barker at
rel...@earthlink.net or toll free at 866-607-3542.
 
*Thanks for your time and Have a great day!* 

Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

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[Histonet] RELIA Histology Job Alert 12/06/10 Histotech needed in Coastal Texas - Private Pathology Lab ASCP or eligible. Can you help?

2010-12-06 Thread Pam Barker
Hi Histonetters!!
I hope everybody is having a great day.  I am working with a client on a new
position that I wanted to tell you all about.
My client is a private pathology lab located in Coastal Texas.  This is a
dayshift (early morning) full time permanent position.  It is M-F with
occasional Saturdays.  My client offers a competitive pay, benefits and
relocation assistance.  
Requirements:
ASCP or elig.
1-2 yrs plus Routine Histology experience (including hand staining)
IHC is a plus.
This is an immediate opening but my client is willing to consider candidates
who prefer to start work after the holidays as well.  If you or anyone you
know might be interested please contact me - Pam Barker at
rel...@earthlink.net or toll free at 866-607-3542.
 
*Thanks for your time and Have a great day!* 

Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

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RE: [Histonet] Problems with histonet

2010-12-06 Thread sgoebel
I haven't received anything since Wednesday, but my email is blowing up
now with Histonet stuff!  I thought it was just my computer =)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi
Allison
Sent: Friday, December 03, 2010 6:54 PM
To: histonet
Subject: [Histonet] Problems with histonet

I just made 2 posts today and they are not showing up.  Is any one
having 
problems too?
 Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com


  
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RE: [Histonet] Competencies

2010-12-06 Thread Morken, Tim
We keep ours for two years. We are under JC and that is how far back they go 
when checking.

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Wilder
Sent: Thursday, December 02, 2010 10:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Competencies


When a Histotech leaves their place of employment - does anyone know if their 
competencies need to be kept for any period of time or can the competencies be 
disgarded after their departure?  Thanks for your help!
 
Paula Wilder
St. Joseph Medical Center 
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RE: [Histonet] Problems with histonet

2010-12-06 Thread Herrick, James L. (Jim)
I made a post this morning as well, and it didn't show. However, I did
receive a response. 

Jim

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi
Allison
Sent: Friday, December 03, 2010 6:54 PM
To: histonet
Subject: [Histonet] Problems with histonet

I just made 2 posts today and they are not showing up.  Is any one
having 
problems too?
 Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com


  
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[Histonet] Veterinary SOPs

2010-12-06 Thread Breeden, Sara
For those of you who work in a veterinary diagnostic lab where there is
a BSL-2 and/or BSL-3 necropsy room, I could use some assistance with
designing some SOPs for maintenance of those rooms.  For example, what
are your daily routines and/or procedures to ensure that those rooms are
clean, temperatures checked, setup requirements met, and so forth.  This
is not writing pathologists' SOPs for specific necropsy procedures, but
more the day-to-day operation and maintenance of the rooms in which a
pathologist would work.  I would appreciate any input and will share
with those who ask.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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[Histonet] Eosin in Tissue Processor

2010-12-06 Thread Akemi Allison
Happy Monday Everyone!

I guess I am having a old persons momment!  I forgot how much eosin and what % 
to put in the last 100% of the Tissue Processor to show-up small transparent 
bx's.  Can one of you wonderful people tell me please.  The processor I am 
using 
is a VIP 5.
 
Thanks a Bunch!

 Akemi Allison BS, HT(ASCP)HTL
Director 
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com 



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RE: [Histonet] Hemoglobin A

2010-12-06 Thread Feher, Stephen
We just got some from BioGenex order number is AR021-5R. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Thursday, December 02, 2010 5:52 PM
To: Histonet
Subject: [Histonet] Hemoglobin A

Howdy histoland,
does anyone know where to get Hemoglobin A? Dako doesn't have it any
more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc,
etc, etc, Biogenex. No luck. Thanks

Joe
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RE: [Histonet] Workload Recording

2010-12-06 Thread Feher, Stephen
If you are set up for your techs to bar code scan their blocks before
they cut, you can keep track of it that way.  We have this set up in our
LIS.  Our techs scan at every station (embedding, cutting, staining,
etc) so I can track specimens in addition to keeping statistics.  


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Friday, December 03, 2010 5:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Workload Recording

Hello to all in histoland.  Does anyone keep a log of how many blocks
that their techs cut.  My boss asked me do I keep this type of
information. Anyone doing workload recording?

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from
your computer system.

To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance
Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR
Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is
confidential and/or privileged.  This e-mail may also be confidential
and/or privileged under Texas law.  The e-mail is for the use of only
the individual or entity named above.  If you are not the intended
recipient, or any authorized representative of the intended recipient,
you are hereby notified that any review, dissemination or copying of
this e-mail and its attachments is strictly prohibited.

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Re: [Histonet] New Histology Field Technical Role Based in AZ/TX/Southern CA

2010-12-06 Thread Kim Tournear
This is currently my territory. I'm relocating to Florida, so if anyone wants 
more details about the job, contact me at this address and we'll touch 
basethis job is fun

~Kim~   
OU ROCKS
~Don't be afraid your life will end, 
be afraid it will never begin~

--- On Thu, 12/2/10, Matt Ward m...@personifysearch.com wrote:


From: Matt Ward m...@personifysearch.com
Subject: [Histonet] New Histology Field Technical Role Based in AZ/TX/Southern 
CA
To: histonet@lists.utsouthwestern.edu
Date: Thursday, December 2, 2010, 9:33 PM


Good Afternoon Everyone!



We have had a world leader open a new Field Technical Histology position
that will cover the Pacific Region. The best place to live for this position
would be Phoenix, Los Angeles, Or Dallas, and it will focus on Core
Histology. This is perfect for a Histotech that is looking for an
opportunity to get out of the lab in into the field with a growing company
that offers promotion.



Please contact me directly by sending me a copy of your resume to
m...@personifysearch.com.



Thanks!



Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

www.personifysearch.com
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Re: [Histonet] Hemoglobin A

2010-12-06 Thread Joe Nocito

thanks y'all for the info

Joe
- Original Message - 
From: Feher, Stephen sfe...@cmc-nh.org
To: Joe Nocito jnoc...@satx.rr.com; Histonet 
histonet@lists.utsouthwestern.edu

Sent: Monday, December 06, 2010 4:15 PM
Subject: RE: [Histonet] Hemoglobin A


We just got some from BioGenex order number is AR021-5R.


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Thursday, December 02, 2010 5:52 PM
To: Histonet
Subject: [Histonet] Hemoglobin A

Howdy histoland,
does anyone know where to get Hemoglobin A? Dako doesn't have it any
more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc,
etc, etc, Biogenex. No luck. Thanks

Joe
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[Histonet] Problems with histonet

2010-12-06 Thread ANJUM PARKAR
I have the exact same problem. I had send out fur posts none showed up.
Anjum

On Mon, Dec  6, 2010 03:35 PM, histonet-requ...@lists.utsouthwestern.edu wrote:

Send Histonet mailing list submissions to
   histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
   http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
   histonet-requ...@lists.utsouthwestern.edu

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   histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than Re: Contents of Histonet digest...


Today's Topics:

   1. FW: TBS Histology Equipment (Damaris Beil)
   2. Changing solutions on VIP processors. (Angela Bitting)
   3. Eosin in Tissue Processor (Akemi Allison)
   4. Workload Recording (Scott, Allison D)
   5. Eosin in Tissue Processor (Akemi Allison)
   6. Problems with histonet (Akemi Allison)
   7. Test - please disregard (histot...@imagesbyhopper.com)


--

Message: 1
Date: Fri, 3 Dec 2010 13:30:49 -0500
From: Damaris Beil dbe...@hotmail.com
Subject: [Histonet] FW: TBS Histology Equipment
To: histonet histonet@lists.utsouthwestern.edu
Message-ID: snt141-w356cb443b8d0d38a312d7598...@phx.gbl
Content-Type: text/plain; charset=iso-8859-1




 
 
 
 
 
Damaris E. Beil, HT,ASCPcm, HTL
Associates in Dermatology
Histology Laboratory
(407) 846-7546 ext. 3307



 


From: dbe...@hotmail.com
To: histonet@lists.utsouthwestern.edu
Subject: TBS Histology Equipment
Date: Fri, 3 Dec 2010 10:48:04 -0500




Hello everyone,
 
I was wondering if any of you would be able to give me feedback on the TBS ATP
processor, TEC embedding center, SHUR/Stain HE stainer, microtomes and
other piece of  instrument from TBS/SHUR.
 
Thank You,
 
 
 
 
 
Damaris E. Beil, HT, ASCPcm,HTL 

Associates in Dermatology
Histology Laboratory
(407) 846-7546 ext. 3307 
 
 
 

 

 

--

Message: 2
Date: Fri, 03 Dec 2010 14:02:59 -0500
From: Angela Bitting akbitt...@geisinger.edu
Subject: [Histonet] Changing solutions on VIP processors.
To: histonet@lists.utsouthwestern.edu
Message-ID: 4cf8f893.2b7f.00c...@geisinger.edu
Content-Type: text/plain; charset=us-ascii

Hello All,
I'm involved in a debate with a colleague about how frequently the tissue
processors need to be changed.
We do roughly 1000 blocks a day on various runs, utilizing 7 tissue processors.
About 100 of those are microwave processed, so I'm subtracting those from the
equation.
 
So 900 blocks (mixed tissues, no breast) divided by 6 conventional
processors is 150 blocks each.
 
I think it's poor reagent management to change a VIP after 150 blocks. Does
anyone disagree with me?
If these were processing very fatty tissue, I might think differently, but
that's not the case.
 
All opinions are welcome.
Thanks,
Angie
 
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!


IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It
is intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure, copying,
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prohibited and may be unlawful. If you have received this message in error,
please delete all electronic copies of this message (and the documents
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-- next part --
BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Bitting, Angela
TEL;WORK:570-271-6844
ORG:;Histology
EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu
N:Bitting;Angela
END:VCARD


--

Message: 3
Date: Fri, 3 Dec 2010 12:26:39 -0800 (PST)
From: Akemi Allison akemiat3...@yahoo.com
Subject: [Histonet] Eosin in Tissue Processor
To: histonet histonet@lists.utsouthwestern.edu
Message-ID: 374906.66310...@web113814.mail.gq1.yahoo.com
Content-Type: text/plain; charset=iso-8859-1

Happy Friday Everyone,

I guess I am having a old persons momment!� I forgot how much eosin and what
% 
to put in the last 100% of the Tissue Processor to show-up small transparent 
bx's.� Can one of you wonderful people tell me please.� I tissue processor
is a 
VIP 5.

Thanks a bunch!
Akemi Allison BS, HT(ASCP)HTL
Director 
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com 


  

--

Message: 4
Date: Fri, 3 Dec 2010 16:09:36 -0600
From: Scott, Allison D allison_sc...@hchd.tmc.edu

[Histonet] clearium

2010-12-06 Thread kimberly muska
    Does anyone have any experience with the Surgipath product 
“Clearium”? It is a mounting medium that clears and coverslips in “one easy 
step” eliminating the need for xylene or xylene substitutes following staining. 
Does it decrease the shelf life of the slides? Is it very expensive? In my lab 
we use a microwave processor so if I used this product I would have no need for 
Xylene or substitutes at all.
Thanks, Kim Ruth HT(ASCP)



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RE: [Histonet] Hellerstrom Hellman anyone?

2010-12-06 Thread Amos Brooks
Thanks,
I did, with 50% nitric acid. I would have thought that if there was a 
problem 
with the glassware it would have shown up in the silver nitrate overnight. The 
silver solution was clear after incubating. The milky mess didn't happen until 
after washing in 95 ETOH then placing in the developing solution. Just to be 
sure though I'll make sure to clean the glassware longer than last time. I'll 
also run a couple of controls and take one out after an hour to reduce just it 
really is over impregnated.

Thanks again,
Amos

Message: 16
Date: Wed, 1 Dec 2010 20:58:20 -0600
From: Ingles Claire  cing...@uwhealth.org
Subject: RE: [Histonet] Hellerstrom  Hellman anyone?
To: histonet@lists.utsouthwestern.edu
Message-ID:
f2f030053f9b7345831bed293a6d57e103a1a...@uwhc-
MAIL01.uwhis.hosp.wisc.edu

Content-Type: text/plain;   charset=iso-8859-1

Did you acid clean your glassware before hand?
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Amos Brooks
Sent: Wed 12/1/2010 12:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hellerstrom  Hellman anyone?



Hi,
I just did a Hellerstrom  Hellman alcoholic silver stain for 
pancreatic D-
cells. I followed the method described by Bancroft  Stevens. When the slides
were placed into the reducing solution they instantly precipitated into a
toasty mess, the solution turned milky brown and the slides (even around the
sections) picked up a lot of silver precipitate. If anyone has done this of
could offer suggestions about it, I'd really like to trouble shoot the stain a
bit.
A bit of background about the stain that I thought I should add. 
According to
the published method I used a 10% silver solution in ETOH with 100ul of 1M
nitric acid. The pH of the solution was 5.0. The reducing solution was
alcoholic formalin with 5% pyrogallic acid.
I thought the silver solution seemed really concentrated (It hardly 
even
disolved). Incubating such a solution overnight at 37 deg C (as indicated in
the method) seemed a bit much. Has anyone heard of modifications of this
stain? There is no sense reinventing the wheel, but I certainly don't want to
repeat it knowing that it will innevitably do the same thing again unless
there is something glaringly obvious that I did wrong.

Thanks,
Amos

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RE: [Histonet] Workload Recording

2010-12-06 Thread WILLIAM DESALVO

There are now several commercial workload/work flow management systems 
available, if you are bar coded for your blocks and slides (Ventana, Thermo, 
Leica and Dako). These automated  processes can simplify the capture of 
metrics for individuals, but not many have robust report functions. You could 
also use a manual system for capturing metrics, but this approach will require 
some additional effort over and above what is required for the automated 
systems. Both approaches will provide you a detailed look at what can be done, 
what is being done and what should be done at each step of your process and by 
whom. Productivity is a key management tool and with all the economy and 
industry pressures being applied, now being looked at closely for the Histology 
lab. 
 
I have been manually capturing workload metrics for accessioning, grossing, 
embedding and microtomy for 7+ years. It can be very useful when working with 
management to develop the correct staffing needs for the manual tasks in 
Histology. Workload recording allows you to better manage your process and 
employees. The data collected can be sliced and diced in many ways and I 
strongly suggest you collect on a daily basis. Each employee should know 
exactly what is expected of them for productivity and quality and you can only 
provide that direction when you have useful data. Once you have the data and 
create your reports, make sure you communicate with all employees regularly and 
continually.
 
All that said, what are you trying to accomplish with creating workload metrics 
and/or what does your boss want to accomplish? It is important to understand 
that just capturing the workload metrics mean nothing without coupling quality 
standards. I believe that since we are very manual in our unit 
(case/specimen/block/slide) production, you must not just look at the 
production factor, you must apply a quality factor/standard, before setting any 
production standards. Quality must come first. last and always in your process 
development. Of course, that will mean you will need to take the next step and 
develop a process for capturing, trending and correcting all Mislabel, 
Procedural and Technical errors produced at each step of the process and ensure 
that the production metric and quality metric, when combined will lead and 
direct you to improved quality and measurable standards.
 
I find that having the workload and quality metrics available provides valuable 
information for development of staff, training/competency documentation, SOP's 
and justifying equipment, consumables and reagent purchases. I learned from my 
CEO that You cannot manage what you do not measure. I would be more than 
happy to discuss this process with you off line, just send me an e-mail to 
start the process.

William DeSalvo, B.S., HTL(ASCP)
wdesalvo@hotmail.com



 
 Date: Fri, 3 Dec 2010 16:09:36 -0600
 From: allison_sc...@hchd.tmc.edu
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Workload Recording
 
 Hello to all in histoland. Does anyone keep a log of how many blocks
 that their techs cut. My boss asked me do I keep this type of
 information. Anyone doing workload recording?
 
 Allison Scott HT(ASCP)
 Histology Supervisor
 LBJ Hospital
 CONFIDENTIALITY NOTICE:
 If you have received this e-mail in error, please immediately notify the
 sender by return e-mail and delete this e-mail and any attachments from 
 your computer system.
 
 To the extent the information in this e-mail and any attachments contain 
 protected health information as defined by the Health Insurance Portability 
 and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 
 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
 privileged. This e-mail may also be confidential and/or privileged under 
 Texas law. The e-mail is for the use of only the individual or entity named 
 above. If you are not the intended recipient, or any authorized 
 representative of the intended recipient, you are hereby notified that any 
 review, dissemination or copying of this e-mail and its attachments is 
 strictly prohibited.
 
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Re: [Histonet] Greta HT Position

2010-12-06 Thread Gervaip
sounds like a full time+ job 
 
 
In a message dated 12/6/2010 2:29:55 P.M. Central Standard Time,  
mh...@carisls.com writes:



Great opportunity for a Histotechnician in a brand new  laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a  certified HT or
HTL to run their newly constructed laboratory. Bellmeade  Dermatology has
been in the dermatology business for 18 years with 3  physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified  and CLIA
certified to perform gross dissection, prior supervisory  experience
preferred. The candidate will be responsible for the following:  Creation
and maintenance of policies and procedures to CLIA standards,  leading
lab through CLIA inspection, maintenance and quality control  for
equipment, and routine histology duties. This is a part time   position
that offers a competitive salary and  the flexible hours  allows you to
put your own personal stamp on the laboratory .   Interested applicants
should contact Meredith Hale phone 214-596-2219 or  through email
mh...@carisls.com 





Meredith Hale HT  (ASCP) CM

Operations Liaison Director and Education Coordinator  



Caris Life Sciences

6655 North MacArthur Blvd, Irving  Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax:  972-929-9966

mh...@carisls.com mailto:mh...@carisls.com   



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RE: [Histonet] Changing solutions on VIP processors.

2010-12-06 Thread WILLIAM DESALVO

I suggest to develop the best quality standard for processing, you must first 
develop or decide what the quality parameters will be that dictates a rotation 
or changing of a reagent. Have you determined what constitutes a dirty or 
unacceptable reagent? Create and run a test method validation protocol to 
define the quality parameters.
 
Your particular mix of tissues types and the number of cassettes processed are 
important indicators, but what how do you determine when a reagent is 
unacceptable? Have you measured the particulate matter, concentration of 
solution, carryover from one reagent type the the next? Define the parameters 
first and then you can set the standard, for your lab and mix of tissues, that 
will provide consistent and quality results, each and every time. Make sure you 
include the pathologist, as he/she is a critical end point QC for your process. 
Make sure you are meeting your most important customer's needs.
 
I believe that testing and then defining set standards will allow you to best 
manage the reagents used on the processors and the process, while providing the 
best and consistent quality. Making a guess for when the reagents should be 
changed for multiple processors or relying on anecdotal information from labs 
that do not gross the same as our lab, have the same daily tissue sample mix as 
you lab or have the same quality standards you have, can only lead to 
inconsistent and possibly wasteful use of reagents or unacceptable processed 
tissue samples. Use standardization and quality processes to lead you to better 
quality results. That is always the goal.  


William DeSalvo, B.S., HTL(ASCP)
wdesalvo@hotmail.com
 
Date: Fri, 3 Dec 2010 14:02:59 -0500
From: akbitt...@geisinger.edu
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Changing solutions on VIP processors.

Hello All,
I'm involved in a debate with a colleague about how frequently the tissue 
processors need to be changed.
We do roughly 1000 blocks a day on various runs, utilizing 7 tissue processors.
About 100 of those are microwave processed, so I'm subtracting those from the 
equation.
 
So 900 blocks (mixed tissues, no breast) divided by 6 conventional processors 
is 150 blocks each.
 
I think it's poor reagent management to change a VIP after 150 blocks. Does 
anyone disagree with me?
If these were processing very fatty tissue, I might think differently, but 
that's not the case.
 
All opinions are welcome.
Thanks,
Angie
 
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
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RE: [Histonet] Competencies

2010-12-06 Thread WILLIAM DESALVO

You should consider your own HR and all regulatory agencies requirements before 
discarding any employee file information. When we were last inspected by CAP, 
they wanted to see the files for all employees listed on our last submission, 
plus new additions and they wanted 2 years of data.
 
Normally I would say at least two years fro any paperwork, but it is always 
best to ask your employee document specialists, HR. There are many different 
requirements for employee file documentation. If there was a corrective action 
issue, then we are required to keep files longer. If the employee was involved 
with formalin, xylene or other concentration testing, then the employee records 
for the testing results must be kept for 20 years. There are many different 
needs to consider before discarding employee files and HR and sometimes Risk 
Management will need to be involved and make the decision on discard. 

William DeSalvo, B.S., HTL(ASCP)




 
 From: hist...@hotmail.com
 To: histonet@lists.utsouthwestern.edu
 Date: Thu, 2 Dec 2010 18:18:32 +
 Subject: [Histonet] Competencies
 
 
 When a Histotech leaves their place of employment - does anyone know if their 
 competencies need to be kept for any period of time or can the competencies 
 be disgarded after their departure? Thanks for your help!
 
 Paula Wilder
 St. Joseph Medical Center ___
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