[Histonet] Ventana ultra
Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ultra
Please Reply All as I'd like to hear feedback also. Thanks. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Wednesday, April 13, 2011 8:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Renal Catecholamines
Does anyone know any places that perform catecholamines on porcine renal tissue and contact numbers? Lynne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ultra
I've heard that adding slides and/or reagents can add as much as 8 minutes to the run time. Again, that's just what I've heard, don't know if it's fact or fiction ~Kim~ OU ROCKS ~Don't be afraid your life will end, be afraid it will never begin~ --- On Wed, 4/13/11, Sebree Linda A lseb...@uwhealth.org wrote: From: Sebree Linda A lseb...@uwhealth.org Subject: RE: [Histonet] Ventana ultra To: barbara.cr...@lpnt.net, histonet@lists.utsouthwestern.edu Date: Wednesday, April 13, 2011, 2:09 PM Please Reply All as I'd like to hear feedback also. Thanks. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Wednesday, April 13, 2011 8:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana ultra
each of the 30 drawers essentially is it's own run. You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! barbara.cr...@lpnt.net 4/13/2011 9:39 AM Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Benchmark Ultra question - follow up
Thank you all for your replies. After all I have to admit, that the cause was human failure. We found, that the protocols have been changed and noone did remember. That was after a stopped run, where HIER war clicked off for the repetition. And afterward only short HIER was clicked on again instead of mild HIER. stupid me! Bye Gudrun _ Von: Lynn Lee [mailto:lynnlee2...@live.com] Gesendet: Mittwoch, 13. April 2011 04:14 An: gu.l...@gmx.at Betreff: FW: [Histonet] Benchmark Ultra question - follow up One last thought- do you filter the new antibody solution you made EVERY time when refilling the prep kit? If not, there could be some particulates in the diluent causing a blockage also. Hope these suggestions help or maybe you have already checked this out. Lynn Lee _ From: lynnlee2...@live.com To: gu.l...@gmx.at Subject: RE: [Histonet] Benchmark Ultra question - follow up Date: Tue, 12 Apr 2011 20:10:43 -0600 It's possible the flip lid on the prep kit dispenser top is not completed closed and seated properly. When the dispense hammer goes down, the pressure may not be the same, every time if this is the case. I always check the tip of the dispenser for air bubbles before putting on the machine for EVERY one whether it is a prefilled Ventana dispenser or my prep kit dispenser. Finally something could be clogged in a line somewhere. I worked in R D for Ventana for almost 4 years and have since used the XT and Ultra in other labs and never had a problem with incorrect amount of reagent being dispensed. I guess there's a first time for anything though. I would call Ventana Customer Support. They are great! Lynn Lee B.S., HT(ASCP) Tucson, AZ From: gu.l...@gmx.at To: histonet@lists.utsouthwestern.edu Date: Tue, 12 Apr 2011 18:23:27 +0200 Subject: [Histonet] Benchmark Ultra question - follow up Today I made a further experiment. I did two titration runs. On one slide I added the working solution by pipette on the other slide I added it by pushing the filled PrepKit. Both stainings came out beautiful. - There has to be something wrong with the dispension-amount. Ventana is informed, I hope they find the culprit. Bye Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Montag, 11. April 2011 18:48 An: 'Angela Bitting' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] Benchmark Ultra question Yes, that could be true. Some of the PrepKits go harder than others. Perhaps the pressure of the hammer is not big enough. Sometimes the nozzles are not filled equally. (We press always reagens in the nozzle when we prepare the run.) With the Ventana-System there is an additional dilution, because the working-solution is added to a reaction-buffer film under the LCS. Perhaps it makes a difference if you dispense the solution with the pipett-tip under the LCS or if the drop falls on the surface of the LCS. That could be a matter of the LCS-quality, or buffer-quantity , or . I think I'll become mad! Our pathologists have an uncertain unhappiness with the overall staining results, but most of the antibodies work well. Perhaps the majority isn't very sensitiv to small changes in the system and the quality-difference isn't big enough, but some are easier to kill. Regards, Gudrun _ Von: Angela Bitting [mailto:akbitt...@geisinger.edu] Gesendet: Montag, 11. April 2011 18:06 An: gu.l...@gmx.at Betreff: Re: [Histonet] Benchmark Ultra question When I was trained to do titration runs on the BenchmarkXT and Ultras, I was told to titrate 100ul of antibody. I have been suspecting for some time now that the dispensers DON'T dispense a full 100ul. I haven't taken the time to prove it, but your question may have motivated me. That could explain the weak staining. Gudrun Lang gu.l...@gmx.at 4/11/2011 11:48 AM Hi! I have some issues with our new Ultra. Since a few days antibodies, that work usually well, show up very faint. For example the TTF1 (Novocastra). I ordered a new bottle, made a titration and found, that the old titer 1:50 was again well enough. I filled the old PrepKit - and the result was very weak. Then I thought, the PrepKit itself is the culprit. I changed the PrepKit - and again the result was very weak. At that time the working-solution was only three days old. Has anyone an explanation, why the titration works well and the automated dispension doesn't? Regards Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the
RE: [Histonet] Ventana ultra
I currently have one XT and 3 Ultras. I love the Ultras. There is no difference in reagent costs. The only thing that I can say remotely related is that if you keep both platforms, the XT and the ultras have separate CC1, CC2, and LCS. I am fairly certain the contents of the bottle are the same, but the label and part numbers are different. I love the continuous access. I have it set up where the three ultras are 'top loaded' with set antibodies every day. This eliminates bouncing things around all the time. Contact me if you would like anymore info. I would be happy to help. I notice no delay in run times. melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Wednesday, April 13, 2011 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FW: 1 HE slide vs. 2
There is not such a study. Cutting 2 vs. 1 HE/block is merely a preference issue amongst pathologists. Now, if you refer to TWO different levels, that is a different issue. Two consecutive-serial sections from each block should not have a diagnostic impact BUT two different levels, separated by 20-25 sections, may. That is a decision to take by the pathologist. René J. --- On Tue, 4/12/11, Eugenia Thomas eugenia90...@hotmail.com wrote: From: Eugenia Thomas eugenia90...@hotmail.com Subject: [Histonet] FW: 1 HE slide vs. 2 To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 12, 2011, 11:14 PM From: eugenia90...@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: 1 HE slide vs. 2 Date: Mon, 11 Apr 2011 13:06:59 -0400 Good afternoon everyone, Does anyone know of an article or statistics discussing the impact (medical diagnosing) of cutting 2 HE slides per block verses 1 for all routine work? Genia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ultra
I recently got an Ultra about a month ago and doing test runs, I have really enjoyed the continuous feed as well but I have a question...if I deactivate a product from the Ultra's inventory list I notice it's still in my XT's inventory list. So, I have to deactivate everything off of each instrument? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, April 13, 2011 9:45 AM To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana ultra I currently have one XT and 3 Ultras. I love the Ultras. There is no difference in reagent costs. The only thing that I can say remotely related is that if you keep both platforms, the XT and the ultras have separate CC1, CC2, and LCS. I am fairly certain the contents of the bottle are the same, but the label and part numbers are different. I love the continuous access. I have it set up where the three ultras are 'top loaded' with set antibodies every day. This eliminates bouncing things around all the time. Contact me if you would like anymore info. I would be happy to help. I notice no delay in run times. melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Wednesday, April 13, 2011 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ultra
We like ours as well. It's not as continual as we originally thought to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a landing zone in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run). Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, April 13, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu; barbara.cr...@lpnt.net Subject: Re: [Histonet] Ventana ultra each of the 30 drawers essentially is it's own run. You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! barbara.cr...@lpnt.net 4/13/2011 9:39 AM Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Ventana ultra
What we like about the Ultra is, that there's no need for cleaning runs between the runs and that you can take out the bulk and waste containers during the run. We don't use the continious loading, because it doesn't fit to our workplan and because we use various antibodies from run to run. The runs are faster, but more than 2 runs per 8 hour shift and 1 run over night are not possible for us. That's the same workload we did with the XT. There are windows during the run, when you can add new slides and reagens. If the needed reagens in on the instrument, you can add them any time, if there's enough place. But the run will last longer and you have to wait for a window of the longer run to get the reagens off the instrument. Ready stained slides can be taken out independently. But the free pads cannot be filled and runs started, until you have the possibility to add the right reagens. So we decided to stay with patch-workload and real go-away. Bye Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von barbara.cr...@lpnt.net Gesendet: Mittwoch, 13. April 2011 15:39 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Von Kossa Stain
We used the lamp at our cutting stations, coplin jar in our waterbath or on top of the embedder, wrapped in tin foil for 1 hr. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org What is everyone using for their light when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC platforms/Sales Representatives
Hello, We are inquiring about IHC staining platforms and we've already contacted the Leica sales rep for the Bond. I would also like to contact Ventana to get a quote on their system, and I've tried to get in contact with a sales rep in our area but I haven't had anyone returning my calls. If there is a Vantana rep reading this, would you please contact the sales representative for my area and let him/her know we are interested in a quote? We are located in Orange County (Fountain Valley), California. For those who have experience with these two platforms, would you mind taking the time to share with me your views and experiences about the two systems? Thanks so much, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 714.433.1330 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: AW: [Histonet] Benchmark Ultra question - follow up
Augh! I hate when that happens! Gudrun Lang gu.l...@gmx.at 4/13/2011 10:44 AM Thank you all for your replies. After all I have to admit, that the cause was human failure. We found, that the protocols have been changed and noone did remember. That was after a stopped run, where HIER war clicked off for the repetition. And afterward only short HIER was clicked on again instead of mild HIER. – stupid me! Bye Gudrun _ Von: Lynn Lee [mailto:lynnlee2...@live.com] Gesendet: Mittwoch, 13. April 2011 04:14 An: gu.l...@gmx.at Betreff: FW: [Histonet] Benchmark Ultra question - follow up One last thought- do you filter the new antibody solution you made EVERY time when refilling the prep kit? If not, there could be some particulates in the diluent causing a blockage also. Hope these suggestions help or maybe you have already checked this out. Lynn Lee _ From: lynnlee2...@live.com To: gu.l...@gmx.at Subject: RE: [Histonet] Benchmark Ultra question - follow up Date: Tue, 12 Apr 2011 20:10:43 -0600 It's possible the flip lid on the prep kit dispenser top is not completed closed and seated properly. When the dispense hammer goes down, the pressure may not be the same, every time if this is the case. I always check the tip of the dispenser for air bubbles before putting on the machine for EVERY one whether it is a prefilled Ventana dispenser or my prep kit dispenser. Finally something could be clogged in a line somewhere. I worked in R D for Ventana for almost 4 years and have since used the XT and Ultra in other labs and never had a problem with incorrect amount of reagent being dispensed. I guess there's a first time for anything though. I would call Ventana Customer Support. They are great! Lynn Lee B.S., HT(ASCP) Tucson, AZ From: gu.l...@gmx.at To: histonet@lists.utsouthwestern.edu Date: Tue, 12 Apr 2011 18:23:27 +0200 Subject: [Histonet] Benchmark Ultra question - follow up Today I made a further experiment. I did two titration runs. On one slide I added the working solution by pipette on the other slide I added it by pushing the filled PrepKit. Both stainings came out beautiful. - There has to be something wrong with the dispension-amount. Ventana is informed, I hope they find the culprit. Bye Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Montag, 11. April 2011 18:48 An: 'Angela Bitting' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] Benchmark Ultra question Yes, that could be true. Some of the PrepKits go harder than others. Perhaps the pressure of the hammer is not big enough. Sometimes the nozzles are not filled equally. (We press always reagens in the nozzle when we prepare the run.) With the Ventana-System there is an additional dilution, because the working-solution is added to a reaction-buffer film under the LCS. Perhaps it makes a difference if you dispense the solution with the pipett-tip under the LCS or if the drop falls on the surface of the LCS. That could be a matter of the LCS-quality, or buffer-quantity , or . I think I'll become mad! Our pathologists have an uncertain unhappiness with the overall staining results, but most of the antibodies work well. Perhaps the majority isn't very sensitiv to small changes in the system and the quality-difference isn't big enough, but some are easier to kill. Regards, Gudrun _ Von: Angela Bitting [mailto:akbitt...@geisinger.edu] Gesendet: Montag, 11. April 2011 18:06 An: gu.l...@gmx.at Betreff: Re: [Histonet] Benchmark Ultra question When I was trained to do titration runs on the BenchmarkXT and Ultras, I was told to titrate 100ul of antibody. I have been suspecting for some time now that the dispensers DON'T dispense a full 100ul. I haven't taken the time to prove it, but your question may have motivated me. That could explain the weak staining. Gudrun Lang gu.l...@gmx.at 4/11/2011 11:48 AM Hi! I have some issues with our new Ultra. Since a few days antibodies, that work usually well, show up very faint. For example the TTF1 (Novocastra). I ordered a new bottle, made a titration and found, that the old titer 1:50 was again well enough. I filled the old PrepKit - and the result was very weak. Then I thought, the PrepKit itself is the culprit. I changed the PrepKit - and again the result was very weak. At that time the working-solution was only three days old. Has anyone an explanation, why the titration works well and the automated dispension doesn't? Regards Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ IMPORTANT WARNING: The information in this message (and the documents attached to it, if any)
RE: [Histonet] Ventana ultra
We load our Ultras with the most commonly used antibodies each morning. So that minimizes the number of landing zones we need to use. Also, (if you run both XTs and Ultras) run your antibodies with the longest protocols on Ultra and that will shorten your XT runs. Setlak, Lisa lset...@childrensmemorial.org 4/13/2011 10:54 AM We like ours as well. It's not as continual as we originally thought to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a landing zone in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run). Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, April 13, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu; barbara.cr...@lpnt.net Subject: Re: [Histonet] Ventana ultra each of the 30 drawers essentially is it's own run. You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! barbara.cr...@lpnt.net 4/13/2011 9:39 AM Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. - This message was secured by ZixCorp(R). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slide brite and coverslip drying time
Hello all. We have just made the transition from xylene in our linear stainer to Slide Brite. We are also coverslipping from Slide Brite with Permount. So far the slides look good (though we have to be careful about water contamination in the dehydrating alcohols) but we are having a problem with extremely long coverslipping drying time. Some of the slides have been on the warmer for over 18 hours and you can still move the coverslips! (Normally the slides would dry within 4 hours on the warmer with xylene and acrymount) Any suggestions on how to speed drying time? Thanks, Dawn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue transfer
Hello all, We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions? Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] General Data Cassette Labeler
Can anyone that uses the General Data cassette labeler give me information regarding your overall opinion of this instrument. 1. I would like to have any information regarding the speed of machine. When we did a demo of this equipment it seems fast. 2.I was also questioning the cassettes, we have found that the only company to purchase these from is general data (at a high cost). 3. Has anyone had the equipment break down and how is the dependability and the customer support service. 4.Our company is very interested in the on demand version. How has anyone found this to be an advantage. 5. Does anyone purchase the cassettes from another dealer besides General Data 6. Has anyone had problems with the black scatching off. 7.We currently use TBS and they are very slow and constantly break down this does not meet our demand as we currently print approx. 400-500 per day. I would like to hear your opinions. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue transfer
I use Harleco Krystalon liquid coverslip medium. Cover the tissue, laying horizontally in oven for a few hours until it hardens, than soak in water bath, peel, and transfer to charged slide with same side facing down, dry in oven again, dissolve in xylene when ready for staining. Diane Craft Pathology Department Animal Medical Center 510 East 62nd St New York NY 10065-8314 212-329-8675 (phone) 212-759-5878 (fax) Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Sent by: histonet-boun...@lists.utsouthwestern.edu 04/13/2011 01:06 PM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] Tissue transfer Hello all, We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions? Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
As long as possible. I remember reading somewhere about TB in lung tissue that was still viable after *years* of 10% NBF. Make sure the lungs are thoroughly perfused, or they will still be unfixed in the middle. There is a technique to inflate lungs at autopsy using a large syringe full of NBF and a clamp. Make sure that the specimen is not contacting the walls or bottom of the specimen container. You can suspend the whole organ with string inside the container, if needed. One can also use gauze or paper towels to keep the organ under the surface of the fixative. Lung tissue is notoriously difficult to fix properly, due both to the high lipid content of the pleural parenchyma, and the fact that fresh lungs float. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slide brite and coverslip drying time
That is exactly one of the problems with Slide Brite, and is the trade-off you have to pay. The alternative (although it may seem odd) is to get away with alcohols and xylene altogether. After staining the slides, wash them in distilled water, shake them well and place them in an over at 60ºC for 5 minutes. After drying apply the mounting medium more diluted (consistency of light mineral oil), and coverslip. You will get away with alcohols, and xylene (or its substitutes). Do not frown, just try it! René J. --- On Wed, 4/13/11, Dawn Herron dwenze...@gmail.com wrote: From: Dawn Herron dwenze...@gmail.com Subject: [Histonet] slide brite and coverslip drying time To: Histonet@lists.utsouthwestern.edu Date: Wednesday, April 13, 2011, 12:16 PM Hello all. We have just made the transition from xylene in our linear stainer to Slide Brite. We are also coverslipping from Slide Brite with Permount. So far the slides look good (though we have to be careful about water contamination in the dehydrating alcohols) but we are having a problem with extremely long coverslipping drying time. Some of the slides have been on the warmer for over 18 hours and you can still move the coverslips! (Normally the slides would dry within 4 hours on the warmer with xylene and acrymount) Any suggestions on how to speed drying time? Thanks, Dawn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions. René J. --- On Wed, 4/13/11, Thurby, Christina christina.thu...@bms.com wrote: From: Thurby, Christina christina.thu...@bms.com Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, April 13, 2011, 2:04 PM Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAB staining for Liver frozen sections
Hi, I need some information on how to block endogenous peroxidase effectively in liver frozen sections, as I need to do DAB staining. What blocking buffer will work best for blocking in liver frozen sections. I appreciate any information on this issue. Thanks, Reena ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
Hi, We fix 'Category-A' agent infected murine lungs in 10% buffered formalin for 7 days in ABSL-3. We also confirm tissue sterility by plating the tissue homogenates on suitable media before taking the samples out of ABSL-3 facility for sectioning. In your case 7 days should be enough, but it is always better to confirm the sterility of tissue. Thanks, Devender Devender Kumar, D.V.M., Ph.D. Postdoctorate fellow, Center for Immunology Microbial Disease, Albany Medical College, 47 New Scotland Avenue, MC 151 Albany, NY 12208 518.262.6220(LAB) -- -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 13, 2011 3:57 PM To: histonet@lists.utsouthwestern.edu; ChristinaThurby Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? 5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions. René J. --- On Wed, 4/13/11, Thurby, Christina christina.thu...@bms.com wrote: From: Thurby, Christina christina.thu...@bms.com Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, April 13, 2011, 2:04 PM Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue transfer
Mission impossible (successfully, you can always ruin them)! It is better to do the staining after a prolonged oven heating and do it delicately. René J. --- On Wed, 4/13/11, Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Subject: [Histonet] Tissue transfer To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, April 13, 2011, 1:06 PM Hello all, We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions? Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Part-time Histotech Opening
Dear Histonetters, We have the following opening: HISTOTECHNOLOGIST ProPath, a high volume, pathology practice, located in Dallas, Texas, has an immediate opening for a part-time Histotechnologist. Responsibilities include embedding tissue specimens, microtomy of paraffin-embedded tissue, operation of automated stainer and coverslipper, equipment maintenance and record retention. The ideal candidate will have a high school diploma or equivalent. We prefer, HT, HTL (ASCP) registered or eligible. The hours are 2:00 p.m. to 6:00 p.m. Monday - Friday. For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 FAX: 214/237-1825 Job-Line: 214/237-1775 Email address: j...@propath.com Website: www.propath.com http://www.propath.com/ Don't Follow the Leader! Join the Leader! EOE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Update regarding question on Plastic Embedding
Mahesh- If you have access to a sonicator, you can etch the slides by first soaking them in 1% formic acid in the sonicator for 5-10 minutes, then after a brief water rinse soak them in 50% ethanol for another 5-10 minutes in the sonicator. You may have to work with the staining times, but you will find that many of your paraffin embedding stains will work. Good luck! Joe Saby, BA HT NAMSA From: Mahesh Polavarapu polavarapu.mah...@gmail.com To: histonet histonet@lists.utsouthwestern.edu Sent: Tue, April 12, 2011 8:25:30 PM Subject: [Histonet] Update regarding question on Plastic Embedding Looking for a protocol to visualize vascularization and collagen deposition at the bone-tendon interface of a rabbit rotator cuff embedded in a plastic system. Sections will be rather thick (~50um) b/c they are being made through a titanium anchor. Using MMA with a cold-curing resin, Technovit 9100. Thanks in advance! - Mahesh ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seeking techs all over the place!
Help! We're getting busy again (the economy rebounds??) and need both permanent and temporary histotechs. Are you looking for... ...a new place to live? ...a bigger city? ...a smaller city? ...a promotion? ...a raise? ...a place to learn new things? We will actively consider... ...new grads (and almost grads) ...experienced bench techs ...bench techs looking for a step up ...experienced management ...retirees (we know you've still 'got it') We have a number of permanent openings and seeking to successfully fill 9 thirteen-week temp positions. We are working histotechs so the conversation is easy and usually kinda fun. Send a resume or ask for an application - tkngfl...@yahoo.com Fax/Phone: 800.756.3309 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Supervisor ( Hospital Scientist)-F/T
A Histology Supervisor ( Hospital Scientist) -F/T position is currently available at The Department of Forensic Medicine (Sydney local health network / Sydney south West), GLEBE 2037 NSW AUSTRALIA. To enquire please check the NSW health web site on http://www.health.nsw.gov.au/jobs/index.asp The successful applicant will be responsible of the supervision of the DOFM histology laboratory which involves the collection and processing of forensic histological specimens. mailto:fawaz.zou...@sswahs.nsw.gov.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
