RE: [Histonet] Input needed
Hi Mary, I was made redundant after almost 40 years with the UK's Medical Research Council at the age of 60 in 2008, I applied for several jobs, was offered interviews for all of them, got the first one at the local university, the down side is that I am on a contract, which has been renewed twice so far but now runs out in August this year and I am not so hopeful as money is tight, so go for it Mary, you have many years of experience and skills and much to offer a future employer and new colleagues. Cheers Richard Edwards -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mary Sent: 21 April 2011 23:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Input needed What’s the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biopsy Run
Morning and Happy Hump day Histo peeps Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... Just need to ask one more question regarding my evil nuclear artifact. We have decided it is a processing issue due to the type of cassettes we use. In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes. I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them. My next thought is to lengthen my biopsy run. Would you folks out there share your biopsy run times with me? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here. Thanks in advance and again hope everyone is having a great Lab week. Kim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] In vivo staining
I am searching for a method to detect how far a dye will penetrate into the skin after applying different barrier disruption methods. My hope was to be able to detect how far into the epidermis the dye penetrates by looking at the samples in a microscope. Do any of you have suggestions on which dye I could use and a protocol that is likely to work for this purpose? The simpler the better! Thanks. Tatiana This e-mail, inclusive of attachments, is intended for the person(s) stated above and may contain confidential information. Unauthorised reading, disclosure, copying, distribution or use of this information may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete your copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Biopsy Run
Mesh cassettes are known to hold air pockets in them, so extending your times would only over process your tissue. I would suggest you use sponges, filter paper, or bx bags and use the cassettes you use for your larger specimens until you can get a different cassette. We found in our lab (we didn't have a printer) that the ink will fade if it's not dry enough before blocks are put in formalin container. Same on the slides. Hope this helps. Sent from the iPhone of Kim Tournear On Apr 27, 2011, at 7:21 AM, Marshall, Kimberly K kkmarsh...@anthc.org wrote: Morning and Happy Hump day Histo peeps Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... Just need to ask one more question regarding my evil nuclear artifact. We have decided it is a processing issue due to the type of cassettes we use. In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes. I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them. My next thought is to lengthen my biopsy run. Would you folks out there share your biopsy run times with me? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here. Thanks in advance and again hope everyone is having a great Lab week. Kim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slide stainer repair
Our slide stainer died this morning and I was wondering if anyone knows of a company that repairs the Zeiss HMS Programmable Slide Stainer? It is over 20 years old and may be beyond repair. I am in Central Pennsylvania. Thanks Roberta Horner HT/HTL Animal Diagnostic Lab Pennsylvania State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biopsy Run
And one more thing Soak the sponges in formalin... j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yahoo Sent: Wednesday, April 27, 2011 08:56 To: Marshall, Kimberly K Cc: Histonet Subject: Re: [Histonet] Biopsy Run Mesh cassettes are known to hold air pockets in them, so extending your times would only over process your tissue. I would suggest you use sponges, filter paper, or bx bags and use the cassettes you use for your larger specimens until you can get a different cassette. We found in our lab (we didn't have a printer) that the ink will fade if it's not dry enough before blocks are put in formalin container. Same on the slides. Hope this helps. Sent from the iPhone of Kim Tournear On Apr 27, 2011, at 7:21 AM, Marshall, Kimberly K kkmarsh...@anthc.org wrote: Morning and Happy Hump day Histo peeps Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... Just need to ask one more question regarding my evil nuclear artifact. We have decided it is a processing issue due to the type of cassettes we use. In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes. I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them. My next thought is to lengthen my biopsy run. Would you folks out there share your biopsy run times with me? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here. Thanks in advance and again hope everyone is having a great Lab week. Kim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Two Questions
1st. Does anyone know if there is a rule or law that states a lab must have a door? 2nd Does anyone know how a TC only lab would do profiency testing on HE slides. They do not have physician on staff and no microscope. Does the company that is reading PC lab do profiency testing on the QC slides and share results with the TC side? Any thoughts would be appreciated. Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biopsy Run
I use the sponges and I keep them in a water so the formalin can penetrate when placed in the processor. Make sure when you put the sponges in the water that you mash them to get out the air bubbles. I use water just in cause my little bowl spills. If it was in formalin I would have to break down my whole grossing station and turn on hood. You can also use kim wipes. Just cut you some squares and place tissue in middle then fold and put in cassette. Hope this helps. Nicole And one more thing Soak the sponges in formalin... j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yahoo Sent: Wednesday, April 27, 2011 08:56 To: Marshall, Kimberly K Cc: Histonet Subject: Re: [Histonet] Biopsy Run Mesh cassettes are known to hold air pockets in them, so extending your times would only over process your tissue. I would suggest you use sponges, filter paper, or bx bags and use the cassettes you use for your larger specimens until you can get a different cassette. We found in our lab (we didn't have a printer) that the ink will fade if it's not dry enough before blocks are put in formalin container. Same on the slides. Hope this helps. Sent from the iPhone of Kim Tournear On Apr 27, 2011, at 7:21 AM, Marshall, Kimberly K kkmarsh...@anthc.org wrote: Morning and Happy Hump day Histo peeps Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... Just need to ask one more question regarding my evil nuclear artifact. We have decided it is a processing issue due to the type of cassettes we use. In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes. I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them. My next thought is to lengthen my biopsy run. Would you folks out there share your biopsy run times with me? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here. Thanks in advance and again hope everyone is having a great Lab week. Kim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Two Questions
On 4/27/11, Nicole Tatum nic...@dlcjax.com wrote: 1st. Does anyone know if there is a rule or law that states a lab must have a door? I suspect that it's not explicitly spelled out, but I'd caution you, if you build a lab without doors, you have to make provisions to provide food and water for the HT's imprisoned inside. It's easier to put in doors. DLS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Two Questions
Hi Nicole, You must get a microscope. Spend $600, get a teaching binocular one. You absolutely can not have a histology lab without a microscope. You must be able to check the quality of your staining and cutting. That being said, the lab that does the PC component should also do QC on your slides. If there are major issues they should address them right away, otherwise, you can set up a QC Form that they fill out quarterly to assure your slides are meeting their standards. -- Katelin Lester, HTL MedSurg Pathology Associates, Inc. (503) 443-2157 On Wed, Apr 27, 2011 at 7:12 AM, Nicole Tatum nic...@dlcjax.com wrote: 1st. Does anyone know if there is a rule or law that states a lab must have a door? 2nd Does anyone know how a TC only lab would do profiency testing on HE slides. They do not have physician on staff and no microscope. Does the company that is reading PC lab do profiency testing on the QC slides and share results with the TC side? Any thoughts would be appreciated. Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Two Questions
Usually I think it has to have a door for fire purposes. Especially since there are so many flammable chemicals in the lab. I could be wrong? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Wednesday, April 27, 2011 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two Questions 1st. Does anyone know if there is a rule or law that states a lab must have a door? 2nd Does anyone know how a TC only lab would do profiency testing on HE slides. They do not have physician on staff and no microscope. Does the company that is reading PC lab do profiency testing on the QC slides and share results with the TC side? Any thoughts would be appreciated. Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Two Questions
Aren't you required to have the lab under negative pressure with respect to the corridor? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, April 27, 2011 10:28 AM To: Daniel Schneider; Nicole Tatum Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two Questions I believe it is a fire law. Even floors that are open, have to have a door at some end near the elevators. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Wednesday, April 27, 2011 10:25 AM To: Nicole Tatum Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Questions On 4/27/11, Nicole Tatum nic...@dlcjax.com wrote: 1st. Does anyone know if there is a rule or law that states a lab must have a door? I suspect that it's not explicitly spelled out, but I'd caution you, if you build a lab without doors, you have to make provisions to provide food and water for the HT's imprisoned inside. It's easier to put in doors. DLS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peloris Processing
Has anyone had issues with processing xylene free on the Peloris? If so, can you share them with me? Has anyone used Leica's xylene substitute Sub-X during processing? Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Biopsy Run
I am afraid your decision is wrong. Empty nuclei appears when the water between the section and the slide is not completely removed and dissolves the nuclear contents while drying and no staining after wards. Check the sections draining for this artifact. René J. --- On Wed, 4/27/11, Marshall, Kimberly K kkmarsh...@anthc.org wrote: From: Marshall, Kimberly K kkmarsh...@anthc.org Subject: [Histonet] Biopsy Run To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 27, 2011, 8:21 AM Morning and Happy Hump day Histo peeps Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... Just need to ask one more question regarding my evil nuclear artifact. We have decided it is a processing issue due to the type of cassettes we use. In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes. I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them. My next thought is to lengthen my biopsy run. Would you folks out there share your biopsy run times with me? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here. Thanks in advance and again hope everyone is having a great Lab week. Kim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Peloris Processing
It is better to dehydrate with 2-propanol and go from 2-propanol to paraffin wax than using a xylene substitute. René J. --- On Wed, 4/27/11, thisis...@aol.com thisis...@aol.com wrote: From: thisis...@aol.com thisis...@aol.com Subject: [Histonet] Peloris Processing To: histonet@lists.utsouthwestern.edu Cc: of...@bioreference.com Date: Wednesday, April 27, 2011, 11:30 AM Has anyone had issues with processing xylene free on the Peloris? If so, can you share them with me? Has anyone used Leica's xylene substitute Sub-X during processing? Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MSH 2011 Symposium
If anyone is interested in attending the annual Mississippi Society for Histotechnology Symposium June 24-26th in Hattiesburg, MS, please contact me directly and I can email you a program! We'd love to have you :) Amber McKenzie, BS, HT(ASCP) MSH Secretary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problem with IMEB
Hi all. Has anyone had difficulties in purchasing refurbished equipment from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now, IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had a similar experience with them??? Thanks for lending an ear (or many). Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problem with IMEB
Hi Sheila, I have not worked with this company in the past but have know about them for many years. I was wondering if you tried talking with John O'Brien the owner of the company. He attends many of the histology meetings and I am sure he would not appreciate his company getting any bad publicity through Histonet. Here is a URL about him and maybe you can use his tag line to your benefit since it reads, We offer you, the customer, 100% product satisfaction. Would love to know what the outcome is. Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshel...@sanfordburnham.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 27, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem with IMEB Hi all. Has anyone had difficulties in purchasing refurbished equipment from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now, IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had a similar experience with them??? Thanks for lending an ear (or many). Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Problem with IMEB
I recently used IMEB for the first time and have had a wonderful experience. My rep Margaret has gone above and beyond to satisfy all my questions and needs. She was always very deligant in getting answers to my questions. The poor women probably had to do four or five quotes before we finally hashed it all out. I have used Belair and Pacific in the past. But, I would give my order to Margaret anytime. Nicole Tatum HT(ASCP) Hi all. Has anyone had difficulties in purchasing refurbished equipment from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now, IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had a similar experience with them??? Thanks for lending an ear (or many). Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MTS/Verhoeff's Stain
Does anyone have a procedure for a combination MTS/VG elastic stain that they would be willing to share? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problem with IMEB
Oops!! Forgot the URL http://www.imebinc.com/aboutimeb.php Here you go! Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshel...@sanfordburnham.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, April 27, 2011 1:55 PM To: Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Problem with IMEB Hi Sheila, I have not worked with this company in the past but have know about them for many years. I was wondering if you tried talking with John O'Brien the owner of the company. He attends many of the histology meetings and I am sure he would not appreciate his company getting any bad publicity through Histonet. Here is a URL about him and maybe you can use his tag line to your benefit since it reads, We offer you, the customer, 100% product satisfaction. Would love to know what the outcome is. Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshel...@sanfordburnham.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 27, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem with IMEB Hi all. Has anyone had difficulties in purchasing refurbished equipment from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now, IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had a similar experience with them??? Thanks for lending an ear (or many). Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Problem with IMEB
John, Thanks for the information. I actually asked for the owner today, he'd previously been out of the country, but was still unavailable. I've repeatedly told them we'd done everything they'd requested but they were still sitting on our money and the instrument. It wasn't until I threatened to cancel that they offered to send the instrument to us immediately. Poor business practice as far as I'm concerned and I'm now very leary of the remainder of their business practices (service, etc...). I hope they come through with a complete refund of our money. I'll be sure to let everyone know how it turns out. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From: John Shelley jshel...@sanfordburnham.org To: Sheila Haas micropathl...@yahoo.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wed, April 27, 2011 2:01:46 PM Subject: RE: [Histonet] Problem with IMEB Oops!! Forgot the URL http://www.imebinc.com/aboutimeb.php Here you go! Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshel...@sanfordburnham.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, April 27, 2011 1:55 PM To: Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Problem with IMEB Hi Sheila, I have not worked with this company in the past but have know about them for many years. I was wondering if you tried talking with John O'Brien the owner of the company. He attends many of the histology meetings and I am sure he would not appreciate his company getting any bad publicity through Histonet. Here is a URL about him and maybe you can use his tag line to your benefit since it reads, We offer you, the customer, 100% product satisfaction. Would love to know what the outcome is. Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshel...@sanfordburnham.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 27, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem with IMEB Hi all. Has anyone had difficulties in purchasing refurbished equipment from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now, IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had a similar experience with them??? Thanks for lending an ear (or many). Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problem with IMEB
I have had excellent luck and service with them. Call Denise deVines she is super nice and helpful =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 27, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem with IMEB Hi all. Has anyone had difficulties in purchasing refurbished equipment from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now, IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had a similar experience with them??? Thanks for lending an ear (or many). Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temporary (8 months) Histology position in San Diego
Hi Everyone, There is a position for a Histotechnologist at one of the major Pharmaceutical companies located in the Torrey Pines/SDSU/La Jolla area. Here is the job description and if anyone knows of someone that is interested please have them contact me. Thank you Dusko Trajkovic 858-638-6202 Temporary Histology position: Position will be for approximately 8 months. It is approved for 40 hours per week but there is some flexibility and as long as there is overlap with a lead histotech the time of day could be flexed. It is an associate scientist III level and will be competitive. This would be a good opportunity for someone who may want to see what industry is like and to also get a crash course in IHC as we do a lot here. It is all non-GLP. Specific responsibilities in assisting the histology lab may include: · Trim formalin fixed rat/mouse organs · Process and embed tissue specimens · Cut frozen and paraffin (mainly) sections · Perform HE stains and special stains as needed · Perform IHC staining manually or by autostainer · Label cassettes and slides · Change reagents in tissue processer and autostainer · Maintain tissue blocks and slide inventories Requirements: · BS in a scientific discipline · 5 years in histology · Tissue processor (VIP), coverslipper, microtome, cryostat, autostainer (DAKO and Sakura) experience · Skills with frozen and paraffin sectioning, HE stains and IHC stains · HT preferred ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Problem with IMEB
As with any other commercial transaction gone sour, contact the Better Business Bureau on the area the company operates, and present a formal and documented complaint. René J. --- On Wed, 4/27/11, Sheila Haas micropathl...@yahoo.com wrote: From: Sheila Haas micropathl...@yahoo.com Subject: [Histonet] Problem with IMEB To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 27, 2011, 1:31 PM Hi all. Has anyone had difficulties in purchasing refurbished equipment from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now, IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had a similar experience with them??? Thanks for lending an ear (or many). Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Searching for someone
I am looking for Cody Best. Does anyone have a way to get hold of him? Thanks in advance! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Automated slide stainer
I just got a pleasant surprise. After asking about repair for my dead stainer I was just told to get quotes for a new stainer ASAP. I need help I haven't looked at stainers for a long time. What is everyone using and how do you like it? Ours is a small lab average 50 slides per day seldom over 80. My old stainer did 50 slides a rack and that is a good capacity for us. Thanks for the information on repairs that I received. Roberta Horner HT/HTL Pennsylvania State University Animal Diagnostic Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Automated slide stainer
Check Sakura stainers René J. --- On Wed, 4/27/11, Roberta Horner r...@psu.edu wrote: From: Roberta Horner r...@psu.edu Subject: [Histonet] Automated slide stainer To: Histonet Histonet@lists.utsouthwestern.edu Date: Wednesday, April 27, 2011, 3:52 PM I just got a pleasant surprise. After asking about repair for my dead stainer I was just told to get quotes for a new stainer ASAP. I need help I haven't looked at stainers for a long time. What is everyone using and how do you like it? Ours is a small lab average 50 slides per day seldom over 80. My old stainer did 50 slides a rack and that is a good capacity for us. Thanks for the information on repairs that I received. Roberta Horner HT/HTL Pennsylvania State University Animal Diagnostic Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Two Questions
Hi, This is probably totally not helpful and I apologize in advance, but do you climb in through the window? I'm thinking the histology lab meets Dukes of Hazard! Sorry about the giggles at your expense but YEE HAW that's funny! Can't wait to see Daisy Duke in a lab coat cut offs! Amos On Wed, Apr 27, 2011 at 10:20 AM, histonet-requ...@lists.utsouthwestern.edu wrote: Message: 24 Date: Wed, 27 Apr 2011 10:12:43 -0400 (EDT) From: Nicole Tatum nic...@dlcjax.com Subject: [Histonet] Two Questions To: histonet@lists.utsouthwestern.edu Message-ID: 3439.208.62.167.196.1303913563.squir...@webmail.realpages.com Content-Type: text/plain;charset=iso-8859-1 1st. Does anyone know if there is a rule or law that states a lab must have a door? 2nd Does anyone know how a TC only lab would do profiency testing on HE slides. They do not have physician on staff and no microscope. Does the company that is reading PC lab do profiency testing on the QC slides and share results with the TC side? Any thoughts would be appreciated. Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diff-Quik stain
Hi Guys, I am looking for diff-quick stain kits that are economical. My pathologist is contemplating another medical mission trip to Haiti and I need to locate a kit that she can take with her for blood smears. I have found a few upwards of $600 but she does not need a gallon of each of the solutions. If you know of a supplier of smaller volumes or even if you have protocols where you make your own, please share. Vendors comments are welcome if you contact me personally, please do not flood histonet with ads. Thanks in advance. Shirley Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Diff-Quik stain
Fisher has a Hema 3 System in 500mL bottles for $144.75 that I use. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell [powell...@mercer.edu] Sent: Wednesday, April 27, 2011 3:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diff-Quik stain Hi Guys, I am looking for diff-quick stain kits that are economical. My pathologist is contemplating another medical mission trip to Haiti and I need to locate a kit that she can take with her for blood smears. I have found a few upwards of $600 but she does not need a gallon of each of the solutions. If you know of a supplier of smaller volumes or even if you have protocols where you make your own, please share. Vendors comments are welcome if you contact me personally, please do not flood histonet with ads. Thanks in advance. Shirley Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Diff-Quik stain
Shirley, I'm not sure if this is what you want, but we just ordered Hema-Diff Rapid Differential Stain(a modified Giemsa/Romanowsky) from StatLabs. Consists of 3 solutions: Soltn 1: Fixative, Soltn 2: Xanthene Dye and Soltn 3: Thiazine Dye. They sell it in gallon size and pint size. The gallon size is $35.00 and the pint size (16oz) is $10.00. Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, April 27, 2011 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diff-Quik stain Hi Guys, I am looking for diff-quick stain kits that are economical. My pathologist is contemplating another medical mission trip to Haiti and I need to locate a kit that she can take with her for blood smears. I have found a few upwards of $600 but she does not need a gallon of each of the solutions. If you know of a supplier of smaller volumes or even if you have protocols where you make your own, please share. Vendors comments are welcome if you contact me personally, please do not flood histonet with ads. Thanks in advance. Shirley Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] power outages for processors
here are usually UPS(uninterrupted Power suppy) batteries available f or just a couple of hundered dollars. The unit plugs into the wall and the = processor plugs into the UPS. Used them for years. Nick(Rocky) Mad= ary, HT/HTL(ASCP)QIHC Joni Madary, PhD(in life) Apr 25, 2011 12:0= 1:30 PM, histonet@lists.utsouthwestern.edu wrote: Send Histonet= mailing list submissions to histonet@lists.utsouthwestern.edu To= subscribe or unsubscribe via the World Wide Web, visit http://lists.uts= outhwestern.edu/mailman/listinfo/histonet or, via email, send a message = with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lis= ts.utsouthwestern.edu When replying, please edit your Subject line s= o it is more specific than Re: Contents of Histonet digest... = BRToday's Topics: 1. Histotech position in Irving, Texas (Gloria Co= le) 2. RE: Processors and power outages (Amber McKenzie) 3. FDA appro= ved IVD antibody (Vickroy, Jim) 4. Happy National Medical Laboratory Pro= fessionals Week (Brian- Prometheus) -- Message: 1 Date: S= un, 24 Apr 2011 15:30:57 -0500 From: Gloria Cole Subject: [Histonet] Histotech position in Irving, Texas To: histonet@l= ists.utsouthwestern.edu Message-ID: 55D857E3-9B4D-4278-9568-E1836A8C= d...@usa.net Content-Type: text/plain; charset=us-ascii Hel= lo, Just wanted to let everyone know I have one and may have another Histotech/ Grossing tech position open in our GI lab in Irving, Texas. The= hours on one position are negotiable the other will be day shift. If you a= re interested or know anyone interested please contact me for more details. Thanks, Gloria Cole BS HT (ASCP) NuePath Supervisor 972-489-2071 gc...@nueterrapathology.com ---= --- Message: 2 Date: Mon, 25 Apr 2011 08:13:07 -0500 F= rom: Amber McKenzie Subject: RE: [Hist= onet] Processors and power outages To: Heather R ,= BR Message-ID: 03C921A1EAF7F5= 41b16543f6ec6a4b3704107...@giamail2.gia.com Content-Type: text/plain= ; charset=iso-8859-1 Back-up battery -Original Messag= e- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Heather R Sent: Saturday,= April 23, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject= : [Histonet] Processors and power outages Wondering what the labs wh= o are not in hospital setting, are doing with thier processors for pow= er outages. Starting a derm lab and want to address this issue. Th= anks H. ___ Histonet m= ailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwes= tern.edu/mailman/listinfo/histonet -= - Message: 3 Date: Mon, 25 Apr 2011 09:02:10 -0500 From: = Vickroy, Jim Subject: [Histonet] FDA approved I= VD antibody To: histonet@lists.utsouthwestern.edu Message-ID: 24A4826E8EF0964D86BC5317306F58A55D f526d...@mmc-mail.ad.mhsil.com Content-Type: text/plain; charset== us-ascii Question - I am trying to figure why we should switch to a= n FDA approved IVD antibody for Cd117 from the current IVD antibody we use = that is not FDA approved. Most of the IHC antibodies we use are not FDA app= roved. I am fully aware of the extra things we have to do if an antibody is= a RUO or ASR antibody but am a little questioned about the true advantage = of the FDA approval. The cost of course for an FDA approved antibody= is extremely high in comparison to other IVD antibodies that are not FDA a= pproved. (as much as five times as much). In this case I am trying to figur e out what is so special about Cd117. If someone could enlighten me = I would be grateful. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medic= al Center 217-788-4046 Th= is message (including any attachments) contains confidential information in= tended for a specific individual and purpose, and is protected by law. If y= ou are not the intended recipient, you should delete this message. Any disc= losure, copying, or distribution of this message, or the taking of any acti= on based on it, is strictly prohibited. = -- Message: 4 Date: Mon, 25 Apr 2011 10:50:39 -0400 From: = Brian- Prometheus Subject:[Histonet]=HappyNational Medical
[Histonet] Re: C4d stain for IF
I'm having trouble with my C4d IF stain. I'm using AbDirect/Serotec, mouse monoclonal diluted 1:500 for 1 hr, and the Vector is AbDirect/Serotec diluted 1:25 for ½ hour. This is not working and I've tried different dilutions with no success. Can anyone share their protocol? Rosie Butler, AmeriPath/3000 United Founders Blvd./Suite 234/Oklahoma City, OK 73112/405.842.7575 - Office 405.650.8921 - Cell/405.841.2002 - Fax CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Biopsy Run
Increase the fixation time. If they are small biopsies (otherwise why would you be putting them in mesh cassettes), you could easily reduce the processing time to 30 minutes (eg 4 alcohols, 2-3 xylenes, 2-3 waxes) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Wednesday, 27 April 2011 10:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy Run Morning and Happy Hump day Histo peeps Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... Just need to ask one more question regarding my evil nuclear artifact. We have decided it is a processing issue due to the type of cassettes we use. In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes. I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them. My next thought is to lengthen my biopsy run. Would you folks out there share your biopsy run times with me? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here. Thanks in advance and again hope everyone is having a great Lab week. Kim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Diff-Quik stain
How about this from PolySciences Inc. $68. http://www.polysciences.com/Core/Display.aspx?pageId=98categoryId=203productId=2865 Looks compact and transportable. Too small? From: powell...@mercer.edu To: histonet@lists.utsouthwestern.edu Date: Wed, 27 Apr 2011 16:52:55 -0400 Subject: [Histonet] Diff-Quik stain Hi Guys, I am looking for diff-quick stain kits that are economical. My pathologist is contemplating another medical mission trip to Haiti and I need to locate a kit that she can take with her for blood smears. I have found a few upwards of $600 but she does not need a gallon of each of the solutions. If you know of a supplier of smaller volumes or even if you have protocols where you make your own, please share. Vendors comments are welcome if you contact me personally, please do not flood histonet with ads. Thanks in advance. Shirley Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Diff-Quik stain
Diff-Quik is a trade name - belongs to whatever Scientific Products is called this week. It's often used generically for all two-stain Romanowsky type stains, since there isn't a really satisfactory generic name for them. I've used several generic stains with separate eosin (xanthine) and blue (thiazine) dye baths, and generally found them satisfactory. But you do need to try them out for yourself. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
