RE: [Histonet] Paraffin blocks from cell line
we made a cell block from cell line by following procedure: - centrifuge cell suspension - fix the sediment overnight in formalin (for tissue fixation) - optional, the button will be anyway fixed by subsequent procedure - centrifuge and decant the formalin - add few drops of liquid agar (not too hot) - as the button solidify you can put it in cassette and process as diagnostic tissue. We got good immunoreactions on sections prepared from melanoma cell line for example. hope this helps Irena Kirbis From: kst...@mcw.edu To: histonet@lists.utsouthwestern.edu Date: Tue, 17 May 2011 13:45:41 -0500 Subject: [Histonet] Paraffin blocks from cell line Hi histonetters, Has anyone made up paraffin blocks from cells from a cell line. This is new to me. I assume they will be in some kind of a media. We will be getting the cells and incubating them to grow. Thanks in advance for the help Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kst...@mcw.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin blocks from cell line
You can use Histogel as well from Richard Allan (Fisher or VWR) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS Sent: Wednesday, May 18, 2011 4:51 AM To: kst...@mcw.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin blocks from cell line we made a cell block from cell line by following procedure: - centrifuge cell suspension - fix the sediment overnight in formalin (for tissue fixation) - optional, the button will be anyway fixed by subsequent procedure - centrifuge and decant the formalin - add few drops of liquid agar (not too hot) - as the button solidify you can put it in cassette and process as diagnostic tissue. We got good immunoreactions on sections prepared from melanoma cell line for example. hope this helps Irena Kirbis From: kst...@mcw.edu To: histonet@lists.utsouthwestern.edu Date: Tue, 17 May 2011 13:45:41 -0500 Subject: [Histonet] Paraffin blocks from cell line Hi histonetters, Has anyone made up paraffin blocks from cells from a cell line. This is new to me. I assume they will be in some kind of a media. We will be getting the cells and incubating them to grow. Thanks in advance for the help Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kst...@mcw.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Filter Paper for Harris Hematoxylin
Hi Dr Dubey Thanks for your message regarding choice of filter papers. Here in the U.K. Whatman No 1 is almost universally used for filtration of all reagents and solutions used in microscopy or general chemistry preparation. Other grades of filter paper are available for other applications, but No 1 is the most popular and enduring simple filtering medium for our purposes. I do not have any particular views on the differences between purchased or 'in-house' prepared Harris Haematoxylin solutions. We use both. We use Sodium Iodate as the ripening agent, this does not appear to change the qualities of the finished solution over the previous use of mercuric oxide. We change our haematoxylin once a week on the stainer, usually it is one of the last jobs at the end of the day to set up the filtering of used haematoxylin in the trough, (usually approx 400ml) so that in the morning there is a flask of crystal free stain to re-use , this does not apply to weekends, as the haematoxylin is discarded early monday morning, the trough washed and dried and fresh haematoxylin added, we run several test slides for evaluation. In addition we always routinely change all the other solutions on the stainer at the start of the week and as required subsequently. Hope this is helpful to you. Alan Taylor BSc(Hons). FRMS. Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. EX1 3AJ On 18 May 2011 05:34, amitapan...@torrentpharma.com wrote: Just continuation of karie query .Do you suggest different filter paper for Lab prepared hematoxylin? What's your view on lab prepared versus ready made Harris hematoxylin? Dr. Amita Dubey PCSED,TRC From: Karie Reaser krea...@vet.upenn.edu To: histonet@lists.utsouthwestern.edu Date: 17/05/11 08:27 PM Subject:[Histonet] Filter Paper for Harris Hematoxylin Sent by:histonet-boun...@lists.utsouthwestern.edu Good morning, What grade filter paper do you use for purchased Harris Hematoxylin filtering? I have used 3 different grades, coarse and still have precipitate on my slides. Any advice is greatly appreciated. Thank you Karie -- Karie L Reaser A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:krea...@vet.upenn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] esterase stain
Good Morning, I'm posting this for a non-member. Does anyone know of a lab that does nonspecific esterase staining? A client needs it done on mouse tissue, and we don't perform that test here in our histo lab. Jan Shivers University of Minnesota Veterinary Diagnostic Laboratory shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Ventana E-cadherin problem
We also experienced tissue adhesion problems. We have done several things to address the issue: We wear gloves when cutting controls to decrease any grease from our fingers, we also dip the slide into the water bath upside down as to leave the portion of the slide for the patient section untouched or un-dipped. Whenever repeating a slide because the first attempt fell off...we always mount the section on a slide all by itself (no control). We have switched to superfrost excel adhesion slides from fisher. (we have also experienced a bad lot of slides):( -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, May 18, 2011 10:05 AM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana E-cadherin problem Sounds to me like you may want to check your slide lots to make sure the charge is consistent. I have purchased slides were there was variability within the back from slide to slide. Also, if you use CC1 and CC2 I would check to make sure that the solutions were not inadvertently switched, since harsher solutions tend to wreak havoc on samples. You may want to pH them as well to make sure they have broken down. Tech support will give you the pH range for each solution if you don't already know them. I hope one of these will resolve your issue. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdess...@wvhcs.org] Sent: Wednesday, May 18, 2011 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana E-cadherin problem Hello Histonet, We've been having a recurring problem with E-cadherin from Ventana on Benchmark Ultra. It's spanned two different lots of antibody. Our tissues are suddenly falling off the slides. Negative controls are ok, just seems to be after antibody treatment. The protocol hasn't changed, it's the manufacturer recommended protocol. It also seems to happen to various tissue types and fixation times. We've tried drying the slides longer and it doesn't seem to help. Anyone else having problems with this antibody? Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any
RE: [Histonet] RE: Ventana E-cadherin problem
We've always had problems with the tissue falling off on the E-Cad (Ventana). Normally, we put the patient tissue on the same slide as the control tissue - but for the E-Cad, we always put the patient tissue on a slide all by itself. This doesn't completely solve the problem, but it's better. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, May 18, 2011 7:24 AM To: Walter Benton; Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Ventana E-cadherin problem We also experienced tissue adhesion problems. We have done several things to address the issue: We wear gloves when cutting controls to decrease any grease from our fingers, we also dip the slide into the water bath upside down as to leave the portion of the slide for the patient section untouched or un-dipped. Whenever repeating a slide because the first attempt fell off...we always mount the section on a slide all by itself (no control). We have switched to superfrost excel adhesion slides from fisher. (we have also experienced a bad lot of slides):( -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, May 18, 2011 10:05 AM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana E-cadherin problem Sounds to me like you may want to check your slide lots to make sure the charge is consistent. I have purchased slides were there was variability within the back from slide to slide. Also, if you use CC1 and CC2 I would check to make sure that the solutions were not inadvertently switched, since harsher solutions tend to wreak havoc on samples. You may want to pH them as well to make sure they have broken down. Tech support will give you the pH range for each solution if you don't already know them. I hope one of these will resolve your issue. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdess...@wvhcs.org] Sent: Wednesday, May 18, 2011 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana E-cadherin problem Hello Histonet, We've been having a recurring problem with E-cadherin from Ventana on Benchmark Ultra. It's spanned two different lots of antibody. Our tissues are suddenly falling off the slides. Negative controls are ok, just seems to be after antibody treatment. The protocol hasn't changed, it's the manufacturer recommended protocol. It also seems to happen to various tissue types and fixation times. We've tried drying the slides longer and it doesn't seem to help. Anyone else having problems with this antibody? Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.
RE: [Histonet] RE: Ventana E-cadherin problem
We treat certain antibodies (including E-Cad, and many other breast markers) by putting the slides into formalin for 15 minutes after baking. Then, rinse in tap and put on the machine. It helps keep tissue on the slides. Also, as an aside, I heard directly from someone who works for Erie (who makes superfrost plus slides)that you cannot remove the positive charge from the slide, no matter how many times you dip it in water - as long as it's plain distilled water, and doesn't have any additives in it (such as Sta-on). That being said, we had many issues with bad lots of slides where the positive charge was uneven, resulting in poor staining more often than with tissue adhesion. Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, May 18, 2011 11:27 AM To: Kuhnla, Melissa; Walter Benton; Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Ventana E-cadherin problem We've always had problems with the tissue falling off on the E-Cad (Ventana). Normally, we put the patient tissue on the same slide as the control tissue - but for the E-Cad, we always put the patient tissue on a slide all by itself. This doesn't completely solve the problem, but it's better. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, May 18, 2011 7:24 AM To: Walter Benton; Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Ventana E-cadherin problem We also experienced tissue adhesion problems. We have done several things to address the issue: We wear gloves when cutting controls to decrease any grease from our fingers, we also dip the slide into the water bath upside down as to leave the portion of the slide for the patient section untouched or un-dipped. Whenever repeating a slide because the first attempt fell off...we always mount the section on a slide all by itself (no control). We have switched to superfrost excel adhesion slides from fisher. (we have also experienced a bad lot of slides):( -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, May 18, 2011 10:05 AM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana E-cadherin problem Sounds to me like you may want to check your slide lots to make sure the charge is consistent. I have purchased slides were there was variability within the back from slide to slide. Also, if you use CC1 and CC2 I would check to make sure that the solutions were not inadvertently switched, since harsher solutions tend to wreak havoc on samples. You may want to pH them as well to make sure they have broken down. Tech support will give you the pH range for each solution if you don't already know them. I hope one of these will resolve your issue. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdess...@wvhcs.org] Sent: Wednesday, May 18, 2011 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana E-cadherin problem Hello Histonet, We've been having a recurring problem with E-cadherin from Ventana on Benchmark Ultra. It's spanned two different lots of antibody. Our tissues are suddenly falling off the slides. Negative controls are ok, just seems to be after antibody treatment. The protocol hasn't changed, it's the manufacturer recommended protocol. It also seems to happen to various tissue types and fixation times. We've tried drying the slides longer and it doesn't seem to help. Anyone else having problems with this antibody? Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views
[Histonet] Leica Bond III
Hi Histonetters I am testing the Leica Bond III immunostainer. How is everyone handling processing the Hercept test from Dako on the Bond III? I cannot use either the approved pretreatment or visualization system, not to mention the approved buffer. I currently run my immunos on the Dako so it has not been a problem. I would appreciate any input anyone has. Thanks in advance. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mission project
Last year I was lucky to be a part of a medical mission in Belize. While there I assisted a dermatologist. This year we are looking for an additional derm dr. (maybe one who feels comfortable reading out slides) and a plastic surgeon to expand on the medical part. There are family practice and ER docs who also are part of the team. On the dental side - there are already numerous dentists and dental techs and hygienists who participate. We also have audiologists and occupational therapists. Dates for the trips are in the fall near the end of October and beginning of November. If anybody knows of a dermatologist or plastic surgeon please ask them to contact me. Belize is a beautiful country and the people are lovely and very friendly. This project is well organized and is well received by Belizeans. Web site is http://www.belizemissionproject.com/main.html Thanks! Andi Grantham U of A, Dept of Cellular and Molecular Medicine 520-626-4415 algra...@email.arizona.edu___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] charge question
For those who stain smears, if you stain with PAS/PAP, would/do you charge for one stain or two. And if you have more that one smear, do you charge for each smear? Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Aperio or similar Slide Scanner
Hello, I am looking for a slide scanner like Aperio to scan my lung tissue slides at high quality and inclusive of increasingly higher magnification objectives. I have the lite version of Aperio to view slides I scanned in when training at MD Anderson in Houston using their Aperio and would like to find something similar in the Dallas area if not at UTSW itself. If you have any suggestions of who has this system or one like it that also allows others to use and/or pay to do so, please let me know. Also, if you have suggestions on purchasing one instead or any other commentaries, I would love to get some feedback. Thank you for your time and assistance, Kathy Kathy Bonness, PhD. kathy.bonn...@utsouthwestern.edumailto:kathy.bonn...@utsouthwestern.edu UTSW Dallas, TX Green Center for Systems Biology Department of Pharmacology Altschuler/Wu Lab ND9.214 mailto:kathy.bonn...@utsouthwestern.edu UT Southwestern Medical Center The future of medicine, today. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking for Aperio or similar Slide Scanner
Kathy We have an Aperio Scanscope XT and scan for a fee Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy Bonness Sent: Wednesday, May 18, 2011 12:23 PM To: histonet@lists.utsouthwestern.edu Cc: Kathy Bonness Subject: [Histonet] Looking for Aperio or similar Slide Scanner Hello, I am looking for a slide scanner like Aperio to scan my lung tissue slides at high quality and inclusive of increasingly higher magnification objectives. I have the lite version of Aperio to view slides I scanned in when training at MD Anderson in Houston using their Aperio and would like to find something similar in the Dallas area if not at UTSW itself. If you have any suggestions of who has this system or one like it that also allows others to use and/or pay to do so, please let me know. Also, if you have suggestions on purchasing one instead or any other commentaries, I would love to get some feedback. Thank you for your time and assistance, Kathy Kathy Bonness, PhD. kathy.bonn...@utsouthwestern.edumailto:kathy.bonn...@utsouthwestern.edu UTSW Dallas, TX Green Center for Systems Biology Department of Pharmacology Altschuler/Wu Lab ND9.214 mailto:kathy.bonn...@utsouthwestern.edu UT Southwestern Medical Center The future of medicine, today. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: 100 micron paraffin sections?
Tim, The thickest paraffin sections I have cut are 50 microns and they curled so tightly right off the blade I can't imagine what you might get with a 100 micron section. Even if you do get this slab intact, I think the tissue would show cracking artifact, not to mention the big chunks taken out at the block face like one would get from too aggressive rough trimming (facing into the block). Sections of this thickness are almost always better if done with a vibratome and mounted out of buffer onto a glass slide. Good luck! Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QC Manual
I am updating my QC Manual and wanted to know if you QC your Processors and Microtomes? These are the only 2 pieces of equipment that I haven't been QC'ing. Here's what I QC each month, am I covering everything? 1. Eye wash station 2. Fridge temp 3. 10%formalin 4. Microscope 5. Processor alarm report 6. Embedder 7. HE stainer 8. Coverslipper 9. Special Stainers 10. Immuno stainers 11. Buffer's 12. Control slides 13. Water baths ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin fixed frozen sections
Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%). Another investigator inquired about doing that. I would appreciate hearing from fellow histonetters. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] QC Manual
If you have a fumes hood, and a body emergency shower you should QC them along with your drying oven microtomes and tissue processor. Any balance you may have also should be QC'd. René J. From: Amber McKenzie amber.mcken...@gastrodocs.net To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 18, 2011 3:36 PM Subject: [Histonet] QC Manual I am updating my QC Manual and wanted to know if you QC your Processors and Microtomes? These are the only 2 pieces of equipment that I haven't been QC'ing. Here's what I QC each month, am I covering everything? 1. Eye wash station 2. Fridge temp 3. 10%formalin 4. Microscope 5. Processor alarm report 6. Embedder 7. HE stainer 8. Coverslipper 9. Special Stainers 10. Immuno stainers 11. Buffer's 12. Control slides 13. Water baths ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re: [Histonet] Formalin fixed frozen sections
Not before embedding in OCT, but we have 10%nbf as our first station in the staining process. The slides stay in there for 1 minute, water rinse, then proceed with your stain as usual. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, May 18, 2011 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin fixed frozen sections Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%). Another investigator inquired about doing that. I would appreciate hearing from fellow histonetters. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin fixed frozen sections
Formalin fixed FS before embedding and sectioning? That is quite confuse. Your FS should be fixed before HE staining (acetone or even 10% NBF will do). If you refer to the specimen that was frozen to do the FS, before being embedded and cut, has to be fixed to become a FFPE specimen. René J. From: Sherwood, Margaret msherw...@partners.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 18, 2011 3:47 PM Subject: Re: [Histonet] Formalin fixed frozen sections Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%). Another investigator inquired about doing that. I would appreciate hearing from fellow histonetters. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Formalin fixed frozen sections
Peggy, We fix ours prior to freezing and cryosectioning routinely. You can use 10% NBF (commercial prep) or you can make your own using paraformaldehyde, 4% in PBS buffer. After fixation, you will need to cryoprotect in sucrose/PBS. We fix, then rinse, and then place in 15% sucrose/PBS until the sample sinks. We then place it in 30% sucrose/PBS until the sample sinks. We will put it into a cryomold with OCT and let sit for some time, then a fresh change and snap freeze. The tissues section very well. Good luck! Teri ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Expiry date of NBF
We use the expiration date on the formalin as the use by date. By the time the solution would be out of date, the tissues should be very well fixed, so the formalin becomes just a holding solution. Joe Saby NAMSA From: amitapan...@torrentpharma.com amitapan...@torrentpharma.com To: histonet@lists.utsouthwestern.edu Sent: Wed, May 18, 2011 12:29:58 AM Subject: [Histonet] Expiry date of NBF Dear Histonetters, I require one clarification on declaration of expiry date of 4% nutral buffer formalin (NBF) prepared in our lab ( from 40% solution) ? Do you declare the expiry date of this prepared solution ? If so what criteria do you follow for this? Important to mention that we have to archive these tissue in NBF for 5 years as part of GLP toxicology. Your experience or view on these points are welcomed. Amita ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech opening in Norman, OK
Job Title: Histology Technician fulltime Porter campus - $3000 Sign On Bonus Department: 40100 -- Laboratory Job Type: Full Time Shift Info: Mon-Fri/ 2 half day Saturday shifts/mon Expertise: Allied Health Job Description: Performs histology procedures including embedding and cutting tissue specimens and preparing and staining microscopic slides. Works with supervisor to assure section is maintained according to regulatory standards. Job Requirements: **Completion of a CAHEA accredited Histotechnology program; or **Associate degree of at least 60 semester hours of academic credit from an accredited college / university including 6 semester hours in chemistry and 6 semester hours in biology; and 1 year full time experience in histopathology under the supervision of a certified pathologist; or **High school graduation or equivalent and two years full time experience in histopathology under the supervision of a certified pathologist. One year of hospital experience as a histologic technician in all phases of histology, or equivalent. Registration as a Histologic Technician (HT) or Histologic Technologist (HTL) by the American society of Clinical Pathologists, or eligibility for registration. Registration is preferred. If interesting, apply on line at www.normanregional.com Adesupo Adesuyi. BS, HT (ASCP) HTL, QIHC (ASCP) Histology Supervisor Norman Regional Health System 405 - 307 -1145 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] haz waste
Does anyone have a letter from there waste water municipality accepting any of the chemicals used in the histo lab (alcohol or neutralized 10%NBF)? Does any one treat there 10%NBF and dispose of it down the sink? Have A Great Day! Sherri Neely ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin fixed frozen sections
We take frozen section from formalin fixed tissue and placed them in 30% sucrose overnight before mounting on chucks. But never tried the other way round frozen tissue then fixed formalin, i heard that it helps in falling of the tissue. But no experience. Amita From: Sherwood, Margaret msherw...@partners.org To: histonet@lists.utsouthwestern.edu Date: 19/05/11 01:21 AM Subject:Re: [Histonet] Formalin fixed frozen sections Sent by:histonet-boun...@lists.utsouthwestern.edu Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%). Another investigator inquired about doing that. I would appreciate hearing from fellow histonetters. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
