[Histonet] Nuclear artefacts in FFPE tissues
I've been attempting to do some multi-colour fluorescence labelling of FFPE lymphoma tissue - usually I only use frozen tissue. It was all going smoothly on some FFPE tonsil sections but with the lymphoma tissues I see punctate nuclear staining with an epitope that should not be nuclear (CD32) - in some samples it appears as punctate staining all over the nuclei but in other samples it appears as a perinuclear halo. It only occurred with one particular antibody (rabbit anti-CD32) and not with rabbit anti-CD20 or mouse anti-CD68. Could this staining just be an artefact - is this common with FFPE tissue and is there something I can do to get rid of it. Thanks Sonya ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] sentinel lymph node melanoma protocols
Good morning everyone, I am curious to see what the standard protocol is on sentinel lymph node for melanomas. I need to establish a protocol at my institution and I have been reading a lot of literature and it seems to be a wide variety of thinking out there from bread loafing the lymph node first then cutting multiple HE sections and IHC in between to bivalving. Some of the protocols call for up to as many as 46 HE sections at 3 microns but it doesn't say where to take the sections for the IHC (S100, pan-melan cocktail). Would any of you like to share your protocols? thank you- Amy Farnan Histology Supervisor Northeast Health Albany, New York Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone Marrow HE Protocols
Hello Histonet! Does anyone have an HE automated stainer protocol for bone marrows that they wouldn't mind sharing? I'm looking at different protocols. It would be greatly appreciated. Thanks. Dawud ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pic of tattooed skin
Good Morning! Found out a few days ago that next week we are participating in a summer camp for high schoolers and it was decided to use skin this year for our examples. I have lots of slides of skin sections from mice, rats, frogs, etc. and even some human skin punches - normal and with skin cancers from when we did a project for a sunscreen manufacturer. What I was looking for was an example of tattooed skin and I spent a bit of time searching for one yesterday online but couldn't find anything that was suitable. Does anyone out there have a picture of skin that has been tattooed? And maybe a good skin scar? Last year's camp was on liver, which was way easier! Thanks. Andi Grantham___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Keyboard and mouse covers
Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Keyboard and mouse covers
I just purchased a keyboard and mouse called Kleen Keys from American Mastertech and they make a washable mouse as well. Our PA is so-so about them...the keyboard is a little sensitive and the mouse is a little bigger than the standard size mouse. Hope this helps- see link below. Lisa http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesitem=EQZ03BK -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, June 01, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keyboard and mouse covers Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Keyboard and mouse covers
We use plastic wrap... Easy to change out and keep clean! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, June 01, 2011 13:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keyboard and mouse covers Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Keyboard and mouse covers
Do you completely wrap your mouse? Or do you leave the ball exposed? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, June 01, 2011 1:16 PM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Keyboard and mouse covers We use plastic wrap... Easy to change out and keep clean! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, June 01, 2011 13:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keyboard and mouse covers Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Keyboard and mouse covers
Actually, we put the mouse in a baggie. But the whole thing is covered. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Wednesday, June 01, 2011 13:19 To: Weems, Joyce; Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: Keyboard and mouse covers Do you completely wrap your mouse? Or do you leave the ball exposed? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, June 01, 2011 1:16 PM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Keyboard and mouse covers We use plastic wrap... Easy to change out and keep clean! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, June 01, 2011 13:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keyboard and mouse covers Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Trichrome stain on frozens
I had major problems with frozen trichrome years ago and Jamie Watson from GNF, La Jolla said to fix in the Bouins and not fix in formalin and then mordant in Bouins. (duh) I am not sure why I never tried that, but when I fixed in Bouins for 10 minutes I got consistent trichrome on frozen skin sections. Donna Harclerode, HT,HTL (ASCP) QIHC, SLS Arista Molecular Immunohistochemist 10455 Pacific Center Court San Diego, CA 92121 858-866-5421 donna.harcler...@aristamolecular.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Keyboard and mouse covers
For the keyboard, what about using the silicon style? A lab near me uses them exclusively. They are easy to keep clean, as they basically watertight - just wipe down with a sani-wipe! And, they are fairly inexpensive - I got one from Office Max (or was it Staples?) for $10. Sent from my iPhone On Jun 1, 2011, at 1:21 PM, Weems, Joyce jwe...@sjha.org wrote: Actually, we put the mouse in a baggie. But the whole thing is covered. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Wednesday, June 01, 2011 13:19 To: Weems, Joyce; Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: Keyboard and mouse covers Do you completely wrap your mouse? Or do you leave the ball exposed? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, June 01, 2011 1:16 PM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Keyboard and mouse covers We use plastic wrap... Easy to change out and keep clean! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, June 01, 2011 13:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keyboard and mouse covers Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain
[Histonet] Re: re-using modified Davidson's solution
Robin Dean asks: Can one re-use modified Davidson's solution for fixing eyes and testes, or has it lost its potency/efficacy after being used previously? Can you rejuvenate it by adding more acetic acid? (how much?) I would think that limited re-use would be acceptable - the proportions of the ingredients in the formula (3 parts water, 3 parts alcohol, 2 parts 37% formalin, 1 part glacial acetic acid) are pretty forgiving. I wouldn't rejuvenate it. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Melanoma sentinel nodes
Amy, I can't find the exact reference but I think either Balch or Taylor authored the paper that was adapted for our protocol. Level 1, 3, 5, 10 are H/E and extra section at level 5 is taken for Negative serum control Level 2 is S100 Level 4, 6 is PanMelanoma cocktail (Biocare Medical) Level 7, 8, 9, are held for as unstained and filed away additional H/E or IHC We usually aim for about 200 microns in between levels. This is entirely dependent on the degree of adipose tissue that was included around the node and the thickness of the node in the paraffin block. When we started this protocol about 5 years ago, we would get 1 or 2 paraffin blocks for analysis. Nowadays we typically receive 3-6 blocks per case (sometimes 2 cases per week) and this has impacted our workload. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 From: Amy Farnan farn...@nehealth.com Subject: [Histonet] sentinel lymph node melanoma protocols To: histonet@lists.utsouthwestern.edu Message-ID: 4de60e2b.26ed.00d...@nehealth.com Content-Type: text/plain; charset=US-ASCII Good morning everyone, I am curious to see what the standard protocol is on sentinel lymph node for melanomas. I need to establish a protocol at my institution and I have been reading a lot of literature and it seems to be a wide variety of thinking out there from bread loafing the lymph node first then cutting multiple HE sections and IHC in between to bivalving. Some of the protocols call for up to as many as 46 HE sections at 3 microns but it doesn't say where to take the sections for the IHC (S100, pan-melan cocktail). Would any of you like to share your protocols? thank you- Amy Farnan Histology Supervisor Northeast Health Albany, New York Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. -- Message: 7 Date: Wed, 1 Jun 2011 09:40:03 -0700 (PDT) From: Dawud AlYasa dawudh...@yahoo.com Subject: [Histonet] Bone Marrow HE Protocols To: histonet@lists.utsouthwestern.edu Message-ID: 759774.67432...@web34506.mail.mud.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hello Histonet! ? Does anyone have an HE automated stainer protocol for bone marrows that they wouldn't mind sharing? I'm looking at different protocols. It would be greatly appreciated. Thanks. ? Dawud -- Message: 8 Date: Wed, 1 Jun 2011 09:54:01 -0700 From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu Subject: [Histonet] pic of tattooed skin To: HISTONET histonet@lists.utsouthwestern.edu Message-ID: 55dcef43-7dd7-4117-a62d-5790da604...@email.arizona.edu Content-Type: text/plain; charset=us-ascii Good Morning! Found out a few days ago that next week we are participating in a summer camp for high schoolers and it was decided to use skin this year for our examples. I have lots of slides of skin sections from mice, rats, frogs, etc. and even some human skin punches - normal and with skin cancers from when we did a project for a sunscreen manufacturer. What I was looking for was an example of tattooed skin and I spent a bit of time searching for one yesterday online but couldn't find anything that was suitable. Does anyone out there have a picture of skin that has been tattooed? And maybe a good skin scar? Last year's camp was on liver, which was way easier! Thanks. Andi Grantham -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 91, Issue 1 *** Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pathology intradepartmental consults
Histonetters: What percentage of anatomical pathology cases in your facility are reviewed by a second pathologist (intradepartmental consult)? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmas...@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] pathology intradepartmental consults
Around 10% (mostly requested by junior partners). René J. From: mwh...@mcleodhealth.org mwh...@mcleodhealth.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 1, 2011 3:59 PM Subject: [Histonet] pathology intradepartmental consults Histonetters: What percentage of anatomical pathology cases in your facility are reviewed by a second pathologist (intradepartmental consult)? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmas...@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] printed requisitions
Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fume hood
What are you using to check your fume hood's with for QC purposes? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fume hood
We have a service come out and inspect our hoods once a year. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, June 01, 2011 2:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fume hood What are you using to check your fume hood's with for QC purposes? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fume hood
We installed small vanes in the area of maximum flow inside the hood and we daily checked that the vanes were moving by the air. As to the actual flow in f/min the hoods were checked annually by an outside company. René J. From: Amber McKenzie amber.mcken...@gastrodocs.net To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 1, 2011 4:31 PM Subject: [Histonet] Fume hood What are you using to check your fume hood's with for QC purposes? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: printed requisitions
We still use paper. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, June 01, 2011 16:13 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] printed requisitions Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica processor
On behalf of a friend who is looking for a new tissue processor. She is asking for information on a Leica ASB300. Any information would be appreciated. Also, any pros cons of a long term lease/option to buy? Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide Volume Inquiry
I'm trying to determine the annual volume of glass slides produced globally for a project I'm working on. I'm having no luck finding any information. Any suggestions would be greatly appreciated!! All the best, Tj Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Leica processor
Sorry, its an ASP300 processor. Thanks From: Bales, Candy A Sent: Wednesday, June 01, 2011 4:05 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Leica processor On behalf of a friend who is looking for a new tissue processor. She is asking for information on a Leica ASB300. Any information would be appreciated. Also, any pros cons of a long term lease/option to buy? Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] pathology intradepartmental consults
Internal consults are in the range of 2-7% in our institution. Some of these are due to mandated second review of all new malignancies. Retrospective review of cases by other pathologists, for example conferences/tumor board/etc, is higher, in the range of 10-14% of cases. Megan K. Dishop MD Department of Pathology, B120 The Children's Hospital 13123 E. 16th Avenue Aurora, CO 80045 Ph: 720-777-4337 Fax: 720-777-7119 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mwh...@mcleodhealth.org Sent: Wednesday, June 01, 2011 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pathology intradepartmental consults Histonetters: What percentage of anatomical pathology cases in your facility are reviewed by a second pathologist (intradepartmental consult)? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmas...@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail is confidential, may be legally privileged, and for the intended recipient only. Access, disclosure, copying, forwarding and distribution by any means is strictly prohibited. If received in error, do not read but delete and e-mail confirmation to the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: pathology intradepartmental consults
Melanie S. White, MT(ASCP), Laboratory Supervisor, Systems/Anatomic Pathology, McLeod Regional Medical Center asks: What percentage of anatomical pathology cases in your facility are reviewed by a second pathologist (intradepartmental consult)? The one group I've worked with that addressed this question was a group of five experienced surgical pathologists who kept very careful records of their internal consults. They maintained an intradepartmental consultation rate of 2.6%, which they thought was optimal for their particular practice situation. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.
I will be out of the office starting 06/01/2011 and will not return until 06/07/2011. Note: For Cytology issues, please call Molly (day) at 8-421-5487 or Eric (eve) at 8-421-5405, For Histology issues, please call the general histology lab 8-421- 5408, Mario 8-421-4961 (day), Kiran 8-421-5404 (late afternoon/eve) or Wanda Lau for Cyto/Histo issues 8-421-5426.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
