[Histonet] Lipofuscin and counterstaining DAb IHC
Dear Histonetters! I have human brain samples (age 70, HUntington disease) and my goal is to quantitate changes in the number of neurons/glia cells with nuclei staining. I use NeuN IHC and Höechst. My first question is: - Throughout the whole sample there are a lot of lipofuscin signal fading the NeuN signal, we are not able to make any microphtograph for cell counting. I tried Sudan black staining and Sigma autofluorescent reagent with the same result. Could you advise some tips and tricks to avoid this? An alternative solution could be DAB IHC for NeuN and counterstaining with nucleus staining. Second question is: - what type of counterstain do you recommend to be able to identify the IHC and normal nucleus staining in the same section? Thank you in advance, best regards Csaba This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lipofuscin and counterstaining DAb IHC
Csaba, I can't answer the fluorescent question but for light microscopic IHC I would stain for the NeuN using peroxidase-DAB followed by a PAS for lipofuscin followed by Mayer's or Harris's haematoxylin. Result is Brown, pink, blue Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of szi...@bio.u-szeged.hu Sent: Thursday, 2 June 2011 4:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lipofuscin and counterstaining DAb IHC Dear Histonetters! I have human brain samples (age 70, HUntington disease) and my goal is to quantitate changes in the number of neurons/glia cells with nuclei staining. I use NeuN IHC and Höechst. My first question is: - Throughout the whole sample there are a lot of lipofuscin signal fading the NeuN signal, we are not able to make any microphtograph for cell counting. I tried Sudan black staining and Sigma autofluorescent reagent with the same result. Could you advise some tips and tricks to avoid this? An alternative solution could be DAB IHC for NeuN and counterstaining with nucleus staining. Second question is: - what type of counterstain do you recommend to be able to identify the IHC and normal nucleus staining in the same section? Thank you in advance, best regards Csaba This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alcian blue quistion
hi every body i have a confusing quistion reagrding Alcian blue pH 1 2.5 stains. suppose you have 100 goblet cell react positive to pH 1 which mean all have sulfated mucin. and 50 cell from other section react positive to pH 2.5 which mean acidic type(sulfated or sialated). why these 50 cell only react to pH 2.5 althogh the other non reacted cells possess acidic sulfated type?? on other word i need to know for what type of mucins alcian B 2.5 react with? thanx all ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] nuclear haziness
We are having an issue with nuclear haziness on prostate biopsies in our small urology laboratory. It occurs randomly, appearing on some levels and not others within the same block. Another scenario is that we sometimes see it occur only in one small area of a core and the remaining tissue in the core looks beautiful. It can occur throughout a case (12 blocks) or not at all. We have checked for obvious problems, i.e. reagent contamination on the processor and stainer, ruled out temperature issues, microtome problems, etc. to no avail. I would appreciate any input/suggestions that would help to alleviate this problem. Thank you, Jean Brinker St. Louis Urological Surgeons St. Peters, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: printed requisitions
We still require a paper req with all specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, June 01, 2011 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] printed requisitions Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] question about Bielschowsky's Silver Stain results
Hello, I have question regarding results I got using the Bielschowsky's Silver Stain for plaques and tangles. The images and questions pertaining to them are up at http://www.ihcworld.com/smf/index.php/topic,6236.0.html . I would greatly appreciate the input from some one who has used this stain before. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@gmail.com tel: +27-84-632-1925 (c) Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica processor
We have one and it's been reliable. It's about 5-6 years old. It's also easy to use. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bales, Candy A Sent: Wednesday, June 01, 2011 4:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Leica processor Sorry, its an ASP300 processor. Thanks From: Bales, Candy A Sent: Wednesday, June 01, 2011 4:05 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Leica processor On behalf of a friend who is looking for a new tissue processor. She is asking for information on a Leica ASB300. Any information would be appreciated. Also, any pros cons of a long term lease/option to buy? Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: printed requisitions
We still use paper reqs. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, June 01, 2011 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] printed requisitions Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Counter top immunos
Hello Histo folks Hope everyone is having a great weekI am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Part Time Job
Hello, I am looking for a part time job to learn histology in the Raleigh/Durham area of North Carolina. Maybe a few hours a week on Saturday or something like that. I am HT registered. Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Counter top immunos
We also do our immunos manually - but we use no kits. I do not promote but do suggest: - Biocare Vulcan Fast Red as chromogen - air dry slide after counterstaining with hematoxylin, then coverslip - KPL for linkers - concentrated antibodies; ready-to-use are good to check to see if the assay works, but shelf-life is longer if concentrated Good luck and have fun! Tresa Goins Histopathology Supervisor Department of Livestock Bozeman, Montana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, June 02, 2011 7:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counter top immunos Hello Histo folks Hope everyone is having a great weekI am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide Volume Inquiry
How are you going to do that? Do you know how many laboratories there are? Do you know the average slides/year for each? Unless you know both parameters you cannot calculate the total national volume. I wish you luck! René J. From: Tonia Richmond tjrichm...@hughes.net To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 1, 2011 5:28 PM Subject: [Histonet] Slide Volume Inquiry I'm trying to determine the annual volume of glass slides produced globally for a project I'm working on. I'm having no luck finding any information. Any suggestions would be greatly appreciated!! All the best, Tj Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] nuclear haziness
Make sure that ALL your slides are properly dried in the oven and that NONE has any remnant of water underneath before going into the oven to dry. René J. From: Jean Brinker j.brin...@att.net To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 2, 2011 6:03 AM Subject: [Histonet] nuclear haziness We are having an issue with nuclear haziness on prostate biopsies in our small urology laboratory. It occurs randomly, appearing on some levels and not others within the same block. Another scenario is that we sometimes see it occur only in one small area of a core and the remaining tissue in the core looks beautiful. It can occur throughout a case (12 blocks) or not at all. We have checked for obvious problems, i.e. reagent contamination on the processor and stainer, ruled out temperature issues, microtome problems, etc. to no avail. I would appreciate any input/suggestions that would help to alleviate this problem. Thank you, Jean Brinker St. Louis Urological Surgeons St. Peters, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Available for sale - Countertop Grossing Station
(1) Mopec MB100 Grossing Station http://media2.mopec.com/media/pdf/MBSeriesGrossingStationBrochure.pdf Excellent condition. Mark Sofferman, President American ReSource Medical 324 West Englewood Avenue Teaneck, NJ 07666 P: 201.833.1550 F: 201.833.1575 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide Volume Inquiry
There is an article on histology workload in the current issue of: Arch Pathol Lab Med Vol 135, June 2011 Just placed on my desk today; I have only glanced at it. It may have some information helpful to you. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tonia Richmond Sent: Wednesday, June 01, 2011 5:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Volume Inquiry I'm trying to determine the annual volume of glass slides produced globally for a project I'm working on. I'm having no luck finding any information. Any suggestions would be greatly appreciated!! All the best, Tj Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] filopodia of Endothelial cells in corpus luteum in mouse ovary
Dear Histonet members: I am interesting in see the filopodia of the endothelial cells in the corpus luteum of mouse. I have stained 25um cryostat sections of ovary with an antibody agaisnt CD31 (PECAM) and alexa fluor and see it in the confocal microscope, but I haven't succes. someone can tell me what can I do? than you so much!! *** Carmen María García Pascual FIVI/INCLIVA/Facultad de Medicina Universidad de Valencia Departamento de P.O.G (Laboratorios) 96.386.40.48 Avd. Blasco Ibañez 17 46010, Valencia *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PA traveler needed
Would any recruiter who has a traveling PA available for 8-10 weeks coverage starting August please contact me off-line? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Counter top immunos
I like the Vector ABC horseradish peroxidase kits, especially with the Nova Red chromogen. Mouse primary antibodies usually give me better results than rabbit or goat primaries. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, June 02, 2011 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counter top immunos Hello Histo folks Hope everyone is having a great weekI am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] laminin and collagen4 in cat and dog
We want to use laminin and Collagen IV to sort out the origin of some spindle cell tumors. Specifically, malignant peripheral nerve sheath tumors reportedly show laminin and collagen IV basement membranes vs. Fibrosarcoma which does not. We need antibodies that will work in both cat and dog. Is there anyone who has had success with these? Do you have any recommendations for these antibodies? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: [IHCRG] laminin and collagen4 in cat and dog
Margaret We have used the Collagen IV listed below on canine but not feline, for laminin we have not used the antibody from abcam on canine or feline but the spec sheet states it works on most mammals. Good Luck Liz Collagen IV SouternBio 0340-01 UNLB Goat Laminin abcam ab11575 Rabbit Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Perry, Margaret Sent: Thursday, June 02, 2011 12:05 PM To: ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: [IHCRG] laminin and collagen4 in cat and dog We want to use laminin and Collagen IV to sort out the origin of some spindle cell tumors. Specifically, malignant peripheral nerve sheath tumors reportedly show laminin and collagen IV basement membranes vs. Fibrosarcoma which does not. We need antibodies that will work in both cat and dog. Is there anyone who has had success with these? Do you have any recommendations for these antibodies? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: hi...@nsh.org or call 443.535.4060. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Counter top immunos
We do ours manually also (about 20 different antibodies). We use a combo of BioGenex and Dako. I like both set ups, but Dako is more expensive to perform than BioGenex. I like that you can buy the in dividual components from BioGenex instead of a whole ket from DAKO. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Thursday, June 02, 2011 1:06 PM To: Marshall, Kimberly K Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Counter top immunos I like the Vector ABC horseradish peroxidase kits, especially with the Nova Red chromogen. Mouse primary antibodies usually give me better results than rabbit or goat primaries. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, June 02, 2011 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counter top immunos Hello Histo folks Hope everyone is having a great weekI am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alternatives to Cytospin?
I will be collecting bronchoalveolar lavage fluid (BALF) from mice and want to do do a diff-quick stain on the fluid to characterize the leukocyte population. My colleagues usually use a cytospin to concentrate cells onto a slide but I'll be at a lab that doesn't have the equipment so I'm looking for alternatives. Could I just put a drop of the BALF onto a charged slide and air dry it? Or treat the BALF like a blood smear? Alternatively, could I fix the cells in...ethanol maybe and then cytospin them the next day when I get back to my lab? Any ideas will be much appreciated. Jerry Ricks Research Scientist University of Washington Department of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Counter top Immunos...
For mouse and rabbit primary antibodies I like the Vector ImmPRESS kits. They save time. For HRP substrates Nova Red looks too dull to me. I have had good results with AEC and also with Vector ImmPACT DAB. Jerry Ricks Research Scientist University of Washington Department of Pathology -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, June 02, 2011 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counter top immunos Hello Histo folks Hope everyone is having a great weekI am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Alternatives to Cytospin?
Jerry, Firstly, I would not recommend fixing in ethanol since cells will shrink and Romanowsky staining will be compromised (the cells stain too darkly since they are smaller). I would use a centrifuge to concentrate the cells and smear like a blood film or if you do not have a centrifuge available, place a drop of the BALF on the slide and either air dry as you suggested (if cell count is low) or treat it like blood (if it looks quite opaque - lots of cells/ other junk). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Friday, 3 June 2011 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alternatives to Cytospin? I will be collecting bronchoalveolar lavage fluid (BALF) from mice and want to do do a diff-quick stain on the fluid to characterize the leukocyte population. My colleagues usually use a cytospin to concentrate cells onto a slide but I'll be at a lab that doesn't have the equipment so I'm looking for alternatives. Could I just put a drop of the BALF onto a charged slide and air dry it? Or treat the BALF like a blood smear? Alternatively, could I fix the cells in...ethanol maybe and then cytospin them the next day when I get back to my lab? Any ideas will be much appreciated. Jerry Ricks Research Scientist University of Washington Department of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: printed requisitions
We currently use paper reqs, but gasp coming soon, we will be going paperless. I am not sure I am going to like this... ;o). Surgical staff will enter the req in the computer, we receive the order the specimen and enter into our AP system. There are going to be growing pains!!! Sent from my iPhone On Jun 2, 2011, at 8:18 AM, Horn, Hazel V hor...@archildrens.org wrote: We still use paper reqs. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, June 01, 2011 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] printed requisitions Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Working Ventana Probe Protocols
Working Ventana Probe Protocols! Heres a good starting point when using the Kappa/Lambda/EBER Ventana Probes on the Ultra: Kappa Probe -Depar 16 min - Enzyme Protease 2 for 4 min, -ISH (Kappa) Probe 4min -Denature @ 85 degrees for 12min -Hybe 1 hour - 3 stringency washes for 8 minutes each -Blue detection for 20 minutes -counterstain 4 min Lambda and EBER Probes Same as Kappa but use the ISH (Lambda and or EBER) Probe 4 minutes. Everything else is the same. I also learned that there is no need to dilute out Ventana's probe as they are predilutes and ready to use. The probe diluent that they sell is just for using other probes. Hopefully this helps everyone running these probes that need a starting point! I am not affiliated with ventana. Marc Shaeffer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bone Marrow HE Protocols
What's up Tena! Long time. The bone marrows will be fixed in B-5. Dawud ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
