[Histonet] Frozen sectioning human spleen
I've been trying to get some frozen sections of human spleen. Usually all the work I do is with mouse tissue and I take the tissues myself and immediately freeze in OCT in isopentane/dry ice. The problem with the human samples is that I have no control over how they are frozen! I have one sample from our path dept which was just put directly in the -80oC freezer the other sample I have is from LN2 storage (I presume it was snap frozen in LN2 after removal). Anyway the sections from both samples are awful! They seem to cut ok but if you do any staining on them they fall apart and any tissue left just doesn't look right - the nuclei look misshapen, fuzzy, stretched and extracted. In sections from the tissue stored in LN2 the nuclei were either missing or didn't take up the dapi at all (except at the very edge of the section). Has anyone had similar problems? I cut 10um sections, air dry overnight, fix 100% acetone 10min. Thanks Sonya ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Helicobacter tissue
Does anyone in Histo- land have extra blocks they would like to trade for either Gram -/+ or Fungus control blocks? Thanks in advance! Dawn Oakes HT ( ASCP ) Olympic Medical Center 939 Caroline Street Port Angeles, WA 98362 360-417-7467 - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] In need of a Methods in Cell Biology chapter
Try interlibrary loan, your library will have access. Geoff On 6/8/2011 6:11 PM, Colleen Forster wrote: Hello Histonetters, I am wondering if anyone out there has the hard copy volumes of Methods in Cell Biology Volume 13, pages 171-193 (which is Chapter 9). This chapter has methodology in micro autoradiography that I really need for a protocol design. The method I need is Techniques for the autoradiography of diffusible compounds by W.E. Stumpf. This volume is from 1976 so it goes way back!! HELP!! anyone. Our medical library does not have this book and to get it on Amazon was $172, too steep for my pocket. Thanks Colleen Forster HT(ASCP)QIHC U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] BRDU
I used pH 6 HEIR instead of HCl/enzyme for BrdU - it works very well on mouse and monkey intestine. Did not switch to HCl after that. But now I prefer to use EdU instead of BrdU (Click-iT kit from Invitrogen). It is more expensive, but much more sensitive, fast, easy to use and well compatible with all range of antigens(for multi-color fluorescence). Unfortunately, there are no enzymatic reaction for EdU - only fluorescence. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of C B Sent: Wednesday, June 08, 2011 8:37 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] BRDU Is anyone using ph6 HEIR instead of HCl/enzyme digestion for BRDU staining? I've read 2 manufacturer datasheets that recommend pH 6 HEIR. I thought the routine pretreatment was HCl/enzyme digestion. We have FFPE rat intestine to stain. Any feeback is appreciated. Cindy Baranowski, HT SJTRI Atlanta, GA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BioGenex vs Ventana
We are looking for anyone who has opinion/experience with BioGenex Xmatrx i6000 and/or Ventana Ultra NexES. Any input is greatly appreciated. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica CV 5030
Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AUTO: Ramona Nelson is out of the office. (returning 06/13/2011)
I am out of the office until 06/13/20= 11. I = will respond to your message when I return. Note: This is an automated response to your message quo t;Histonet Digest, Vol 91, Issue 12= sent on = 6/9/2011 10:00:26 AM. = This is the only notification you will receive whi= le this person is away. _ * ** IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This messa= ge may constitute an advertisement of a BD group's products or services or = a solicitation of interest in them. If this is such a message and you wou= ld like to opt out of receiving future advertisements or solicitations from= this BD group, please forward this e-mail to optoutbygr...@bd.com. * ** T his m= essage (which includes any attachments) is intended only for the designated= recipient(s). It may contain confidential or proprietaryinformationan=d may be subject to the attorney-client privilege or other confidentialit= y protections. If you are not a designated recipient, you may not review= , use, copy or distribute this message. If you received this in error, pl= ease notify the sender by reply e-mail and delete this message. Thank you . ***= Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Compan= y) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica CV 5030
Azer Toluene Based Mountant with the 21 needle Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda [lbla...@digestivespecialists.com] Sent: Thursday, June 09, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica CV 5030
We are using the needle that was supplied with the stainer. We use the Richard Allen mounting medium, preceded by Richard Allen Clear Rite 3 in the last station. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 09, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Frozen sectioning human spleen
Dear Sonya, You need to find out how these people ARE snap freezing, then train them to do it correctly. I have seen Histonet messages where people have success freezing the way you described and had no problems or so it seemed although I do not care for freezer freezing. If the liver is embedded in OCT in a cryomold and the bottom of the cryomold is lowered slowly into LN2, they may have better results. Just don't drop the mold into the LN2 or the OCT/tissue may crack. Freezer freezing at anytime due to slower freezing that creates some huge ice crystal damage e.g. freeze artifact. I will be happy to send you an excellent discussion of freezing artifact from Dr. Charles Scouten if you wish, found on the web. These people may be waiting too long before freezing, or doing some funky little thing to create artifact. Whatever is being done obviously is ruining the nuclei in center of sample but not the edges where freezing is probably occurring much faster. All artifact may not be due to freezing either, although you have experience with murine spleen. The thicker sections at 10 um could be pulling the nuclei out of the tissue itself. Trimming too thick e.g. at a thickness exceeding 15 um or so, can also do the same thing, especially at cold temperatures for this very homogenous tissue. Spleen contains a lot of blood, which doesn't section well. Some suggestion for these problem these spleen samples. If you already do this, you can ignore or review: Correct snap freezing, although you can try this with the blocks from the not exactly known freezing methods. Set cryostat at -16C to -17C. Warmer temperature allows for better sectioning of homogenous spleen, also liver, brain, and spinal cord. Trim the block at thinner coarse setting. Start trim with rapid coarse advance until you get to the OCT, the trim into the tissue with fine approx.15 um increments. Do a final few turns of the flywheel at final 5 um sectioning thickness. This provides a smooth, clean block face with tissue left intact, not with little chunks pulled out. Using a brand new disposable blade, section at 5 um. I assume you use Plus charge slides (you didn't say you used these?) To check that your sectioning is working well, do a quick Hematoxylin stain on a section. Pick up, fix with 95% ethanol, rinse, dip in hematoxylin 15 or more times, rinse, blue and coverslip. Take a look at your morphology to see if you have corrected the problem as nuclei should be intact. Continue with sectioning then proceed with your drying/fixation. Let us know if anything improves, and good luck on training the other labs. Good luck Gayle M. Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James S. Sent: Thursday, June 09, 2011 5:23 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Frozen sectioning human spleen I've been trying to get some frozen sections of human spleen. Usually all the work I do is with mouse tissue and I take the tissues myself and immediately freeze in OCT in isopentane/dry ice. The problem with the human samples is that I have no control over how they are frozen! I have one sample from our path dept which was just put directly in the -80oC freezer the other sample I have is from LN2 storage (I presume it was snap frozen in LN2 after removal). Anyway the sections from both samples are awful! They seem to cut ok but if you do any staining on them they fall apart and any tissue left just doesn't look right - the nuclei look misshapen, fuzzy, stretched and extracted. In sections from the tissue stored in LN2 the nuclei were either missing or didn't take up the dapi at all (except at the very edge of the section). Has anyone had similar problems? I cut 10um sections, air dry overnight, fix 100% acetone 10min. Thanks Sonya ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ Information from ESET Smart Security, version of virus signature database 6191 (20110608) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 6191 (20110608) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 6191 (20110608) __ The message was checked by ESET Smart Security. http://www.eset.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica CV 5030
We use Cytoseal XYL and a 21 gauge nozzle. We were provided a variety of different size nozzles...which one you use depends on which mountant you use. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 09, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica CV 5030
We use Richard Allan Scientific mounting media with a 21 gauge needle. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Thursday, June 09, 2011 9:23 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Leica CV 5030 Azer Toluene Based Mountant with the 21 needle Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda [lbla...@digestivespecialists.com] Sent: Thursday, June 09, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HPV
The pathologist here would like to get HPV up and running in house. I'm just asking for some feed back from everyone that is running HPV (manufacture; clone; protocol). We currently run the Ventana Benchmark XT/Ultra platform. Any help would be greatly appreciated. Andrea This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology position at Caris
Here is a job I am posting for Crystal. Please contact her (info at bottom of message)and not me about it. Thanks Linda M, Histonet administrator Caris Life Sciences, Inc., a leading biosciences company focused on enabling precise and personalized healthcare through the highest quality anatomic pathology, molecular profiling, and blood-based diagnostic services has an opening for a histotechnologist. Essential Job Functions: Histotechnologists will utilize the knowledge necessary to assist pathology professionals with Immunohistochemistry, specimen processing, embedding, slide preparation, microtomy, and staining, special and other technical procedures performed in the laboratory. * Perform routine and non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s), according to policies and procedures. * Duties and tasks are standardized. * Perform routine maintenance and cleaning of equipment and troubleshoot minor equipment failures. Document remedial actions such as repairs or reported tests. * Adhere to laboratory's quality control policies and document all quality control activities. * Ensure all corporate safety, quality control and quality assurance standards are met. * Ensure compliance with all local, federal, CLIA and CAP regulations. Job Requirements: * Bachelor's Degree in Science or equivalent experience * HT/HTL (ASCP) certification preferred but not required. * 1-2 years of experience in position or specialization Locations: Irving, TX; Phoenix, AZ; Boston, MA Crystal Flowers-Graves Manager, Corporate Recruiting Caris Life Sciences 6655 N. MacArthur Blvd. Irving, TX 75039 (214) 596-7003 Direct cflow...@carisdx.commailto:cflow...@carisdx.com www.carisdx.comhttp://www.carisdx.com/ Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. /pre span style=font-weight: bold;Please consider the environment before printing this e-mail/spanbr / br / span style=font-size: 8pt;This e-mail, facsimile, or letter and any files or attachments transmitted with it containsbr / information that is confidential and privileged. This information is intended only for the use of the br / individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further br / disclosures are prohibited without proper authorization. If you are not the intended recipient, any br / disclosure, copying, printing, or use of this information is strictly prohibited and possibly a br / violation of federal or state law and regulations. If you have received this information in error, br / please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at br / priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all br / applicable privileges related to this information./spanbr / br / /html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TMA instrument
Need help to find best TMA instrument available in the histo world. Any feedback or recommendations are welcome. Thank you, Kiran Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica CV 5030
Linda, I addressed this issue before. The smallest gauge needle that Leica supplies for the CV5030 is a 21 gauge needle which we found to be too big (too much mounting media came out and made a mess of the slides). We found a company on the internet that sells re-usable metal needles in all sizes. The link is: www.dispensinglink.com We got a 23 gauge needle (1/2 inch shaft) pk/12 for @$25.00. We tried different mounting media, currently use permount (in a ratio 4 parts Permount : 1 part CitriSolv). Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 09, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica CV 5030
Thanks again for the information Peggy. I did have your email on your needles and mounting media. I was interested in what everyone else was using also since we are now ready to order a different type of mounting media than what we are currently using. -Original Message- From: Sherwood, Margaret [mailto:msherw...@partners.org] Sent: Thursday, June 09, 2011 1:43 PM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica CV 5030 Linda, I addressed this issue before. The smallest gauge needle that Leica supplies for the CV5030 is a 21 gauge needle which we found to be too big (too much mounting media came out and made a mess of the slides). We found a company on the internet that sells re-usable metal needles in all sizes. The link is: www.dispensinglink.com We got a 23 gauge needle (1/2 inch shaft) pk/12 for @$25.00. We tried different mounting media, currently use permount (in a ratio 4 parts Permount : 1 part CitriSolv). Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 09, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HPV
That is what we are using here. We are no longer using the HPV antibody. It never really worked well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [rcar...@harthosp.org] Sent: Thursday, June 09, 2011 12:02 PM To: Andria B Evans; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HPV You need to use in situ hybridization for HPV detection. IHC is not sensitive or specific for routine use. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Evans, Andria B aeva...@lghealth.org 6/9/2011 11:53 AM The pathologist here would like to get HPV up and running in house. I'm just asking for some feed back from everyone that is running HPV (manufacture; clone; protocol). We currently run the Ventana Benchmark XT/Ultra platform. Any help would be greatly appreciated. Andrea This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Grimelius protocol for small animals in NY
Hello All, I am fairly new to the world of special stains. My specialty is immunohistochemistry. But from what I understand, our lab and many other animal labs in New York have been unsuccessful in getting the Grimelius stain to work. Does anybody from New York have a working protocol? Thanks! Diane Craft Pathology Department Animal Medical Center 510 East 62nd St New York NY 10065-8314 212-329-8675 (phone) 212-759-5878 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
