Re: [Histonet] restaining method of wright-giemsa stained peripheral blood slides
Prepare a 1% alcoholic (100 EthOL) solution of acetic acid and immerse (one by one) the smears in it untilll all the color leaches out. Wash them thoroughly in distilled water, and restain them as usual with your Wright-Giemsa solution.René J. --- On Mon, 7/11/11, Shahram Sabeti sabeti_shah...@yahoo.com wrote: From: Shahram Sabeti sabeti_shah...@yahoo.com Subject: [Histonet] restaining method of wright-giemsa stained peripheral blood slides To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Monday, July 11, 2011, 11:24 PM dear fellows, I have some precious blood slides at hand but with a major problem,pallor, because all of them are unmounted and a long time has elapsed from their preparation .their restaining was frightening for me because a fault could result in their deterioration. do you have any experience in this respect.thank you in advance for your help. sincerely yours, sabeti ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Remove me
Could you please remove me from the mailing list? Kind regards, Nele Degryse ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Remove me
Yes, please remove me fromt he mailing list as well.. Julia Koob Technical Co-ordinator, Histology Laboratory North York General Hospital 416-756-6892 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nele Degryse [nele.degr...@ugent.be] Sent: Tuesday, July 12, 2011 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remove me Could you please remove me from the mailing list? Kind regards, Nele Degryse ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This email message and any attachments are intended only for the use of the individual to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the recipient of this email is not the intended recipient (or the employee or agent responsible for delivering the email to the intended recipient), you are hereby notified that any review, dissemination, distribution or copying or other use of this message is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return email and delete this message and attachments from your system. Thank You. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] WASTE DISPOSAL
Histonetters The stericycle chemists came to our place and looked at all our MSDS sheets that go with the Ventana by products, according to him it is not hazardous waste but most of the byproduct is liquid coverslip which is very oily, therefor he suggest we haul it off with the hazardous waste and this is what we are doing. Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Langerin antibody
Hi all! I have been asked by one of our Pathology fellows to see if anyone is performing this antibody-Langerin- and if so, would you be willing to perform it for us.It is not something that we would be adding to our test menu at this time. Please let me know the fee and how many slides you would need. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Remove me
Remove me too. Sent from my Verizon Wireless Phone - Reply message - From: Julia Koob julia.k...@nygh.on.ca Date: Tue, Jul 12, 2011 7:16 am Subject: [Histonet] Remove me To: Nele Degryse nele.degr...@ugent.be, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Yes, please remove me fromt he mailing list as well.. Julia Koob Technical Co-ordinator, Histology Laboratory North York General Hospital 416-756-6892 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nele Degryse [nele.degr...@ugent.be] Sent: Tuesday, July 12, 2011 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remove me Could you please remove me from the mailing list? Kind regards, Nele Degryse ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This email message and any attachments are intended only for the use of the individual to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the recipient of this email is not the intended recipient (or the employee or agent responsible for delivering the email to the intended recipient), you are hereby notified that any review, dissemination, distribution or copying or other use of this message is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return email and delete this message and attachments from your system. Thank You. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Remove me
you have to remove yourself. follow the instructions. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ryrami...@cox.net [ryrami...@cox.net] Sent: Tuesday, July 12, 2011 1:37 PM To: Julia Koob; Nele Degryse; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Remove me Remove me too. Sent from my Verizon Wireless Phone - Reply message - From: Julia Koob julia.k...@nygh.on.ca Date: Tue, Jul 12, 2011 7:16 am Subject: [Histonet] Remove me To: Nele Degryse nele.degr...@ugent.be, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Yes, please remove me fromt he mailing list as well.. Julia Koob Technical Co-ordinator, Histology Laboratory North York General Hospital 416-756-6892 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nele Degryse [nele.degr...@ugent.be] Sent: Tuesday, July 12, 2011 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remove me Could you please remove me from the mailing list? Kind regards, Nele Degryse ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This email message and any attachments are intended only for the use of the individual to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the recipient of this email is not the intended recipient (or the employee or agent responsible for delivering the email to the intended recipient), you are hereby notified that any review, dissemination, distribution or copying or other use of this message is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return email and delete this message and attachments from your system. Thank You. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] What is a great manual mirotome
Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual mirotome
We have the 2235 and some 2135's, and have been satisfied with all of them. Just the usual wear and tear problems seen over time. Do you have Leica service your current microtome? Is it always the same problem? Most service companies will offer a 30 day guarantee on their work. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 2:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual mirotome
I find it hard to believe that you are not getting what you need from the Leica 2235, I don't have that particular model we have two RM2255 and one RM2145 and we just love them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 12:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual mirotome
We use the Leica RM2255, RM2235, RM2165 and RM2265 and we love all of them! We are a research lab and are cutting paraffin and plastic. What are the specific issues that you are having with them? Nikki Nikki M Wahlberg Histology Lab Supervisor Preclinical Sciences Boston Scientific 763-694-5739 wahlb...@bsci.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, July 12, 2011 1:36 PM To: 'lsc...@sfcn.org'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What is a great manual mirotome I find it hard to believe that you are not getting what you need from the Leica 2235, I don't have that particular model we have two RM2255 and one RM2145 and we just love them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 12:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual mirotome
I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] What is a great manual microtomes
We have two Leica's and love them a RM 2255 and a RM2235. No problems. A real work horse! Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 07/12/2011 02:22 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual microtomes
What she said! RM2235 without question! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] What is a great manual mirotome
I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, sdys...@mirnarx.com wrote: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual mirotome
I want my AO820 back! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, July 12, 2011 2:40 PM To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, sdys...@mirnarx.com wrote: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] What is a great manual mirotome
We have tried the Leicas thru the years and found that post 2030's that our arms were not long enough to turn the handwheel without completely straightening our elbows. This led to soreness in our elbows. Their microtomes kept getting larger and larger with the handwheel further back. The 2125 solves that problem for us. We really like ours. - Original Message - From: Patrick Laurie foreig...@gmail.com To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Sent: Tuesday, July 12, 2011 2:40:12 PM Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, sdys...@mirnarx.com wrote: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] What is a great manual mirotome
Leitz 1512. Great workhorse if u can find one Sent via BlackBerry by ATT -Original Message- From: jsjurc...@comcast.net Sender: histonet-boun...@lists.utsouthwestern.edu Date: Tue, 12 Jul 2011 19:47:27 To: Patrick Laurieforeig...@gmail.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome We have tried the Leicas thru the years and found that post 2030's that our arms were not long enough to turn the handwheel without completely straightening our elbows. This led to soreness in our elbows. Their microtomes kept getting larger and larger with the handwheel further back. The 2125 solves that problem for us. We really like ours. - Original Message - From: Patrick Laurie foreig...@gmail.com To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Sent: Tuesday, July 12, 2011 2:40:12 PM Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, sdys...@mirnarx.com wrote: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual mirotome
I actually use a Microm HM315 and have never had issues with it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Tuesday, July 12, 2011 2:47 PM To: Patrick Laurie Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome We have tried the Leicas thru the years and found that post 2030's that our arms were not long enough to turn the handwheel without completely straightening our elbows. This led to soreness in our elbows. Their microtomes kept getting larger and larger with the handwheel further back. The 2125 solves that problem for us. We really like ours. - Original Message - From: Patrick Laurie foreig...@gmail.com To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Sent: Tuesday, July 12, 2011 2:40:12 PM Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, sdys...@mirnarx.com wrote: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] What is a great manual mirotome
I still use AO820s!!! Keep them greased and oiled and they will just keep going, and going, and going Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Bernice Frederick b-freder...@northwestern.edu To: Patrick Laurie foreig...@gmail.com; sdys...@mirnarx.com sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Sent: Tue, July 12, 2011 2:46:28 PM Subject: RE: [Histonet] What is a great manual mirotome I want my AO820 back! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, July 12, 2011 2:40 PM To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, sdys...@mirnarx.com wrote: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] What is a great manual mirotome
The old black ones, gotta love them. Most of the young techs out there probably have never seen one. Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 7/12/2011 12:46 PM, Bernice Frederick wrote: I want my AO820 back! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, July 12, 2011 2:40 PM To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM,sdys...@mirnarx.com wrote: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histobath
Hey All, I am working with a company to develop a replacement for the Histobath. They should have a prototype to me by the end of the week. I am looking for a lab, preferably in the Chicago, that is willing to test and assess the product. Let me know if you are interested. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome knife holder
Thank you Hugh and all others who replied. In fact what I really meant was rather a knife clamp, I guess, as on the older microtomes, where we insert either a regular knife. So my next question is: does anyone still have a knife clamp for either a 2030 Reichert Jung microtome or a Leica microtome. I found those pictures on the web that represent what I am looking for. Thank you again in advance, Liette Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montreal, Qc 514-931-8731, ext 1519 [http://images.dotmed.com/cgi-bin/size.pl?t=2a=3i=779208.jpg][http://websites.labx.com/rankin/pics/3066.JPG] From: Hugh Luk [hlu...@msn.com] Sent: July 12, 2011 4:04 PM To: Liette Tougas Subject: RE: [Histonet] microtome knife holder Liette, The Reichert Jung 2030 came with a choice of disposable blade (see picture) or fixed blade (see link) holders. One...never both:-( http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItemitem=110710454143clk_rvr_id=247196707126 When Leica purchased Reichert, Jung and other microtome lines, they absorbed them into their new units. Meaning, they do not sell parts or encourage repair of older units. We have replaced all of our aging 2030's with 2255's. And sorry, they were disposed of. Rene is correct in his statement, sort of. Technically, there is a blade holder that looks like a long knife for disposable blades; if you had this and the fixed blade holder, you would be fine. I'm guessing Rene's lab used this set-up. Everyone in Hawaii, went the other way. Good luck with parts. Feel free to ask histonet again. Your best bet is to find someone who is getting rid of their unit, as the knife holder should have never worn out. Hugh Hawaii Date: Fri, 8 Jul 2011 07:03:53 -0700 From: rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; ltou...@dawsoncollege.qc.ca Subject: Re: [Histonet] microtome knife holder CC: Knives (not disposable blades) do not need holders. They are used by themselves. René J. --- On Thu, 7/7/11, Liette Tougas ltou...@dawsoncollege.qc.ca wrote: From: Liette Tougas ltou...@dawsoncollege.qc.ca Subject: [Histonet] microtome knife holder To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, July 7, 2011, 4:23 PM Hi again everyone, I also wanted to ask if anyone has, or new if there was ever, a regular knife (not blade) holder for the Reichert Yung 2030 microtome and/or the Leica microtome series. thank you again in advance, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College 514-931-8731, ext 1519 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual mirotome
I paid $30.00 for my pre-owned Spencer 820. Liked it so well I bought two. It has served as a virtually foolproof and indestructible workhorse for 22 years. I have produced, literally more than a tone of slides with it. Jerry L Ricks Research Scientist University of Washington Department of Pathology Date: Tue, 12 Jul 2011 12:57:28 -0700 From: vic...@pathology.washington.edu To: b-freder...@northwestern.edu Subject: Re: [Histonet] What is a great manual mirotome CC: Histonet@lists.utsouthwestern.edu The old black ones, gotta love them. Most of the young techs out there probably have never seen one. Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 7/12/2011 12:46 PM, Bernice Frederick wrote: I want my AO820 back! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, July 12, 2011 2:40 PM To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM,sdys...@mirnarx.com wrote: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histobath revised
Hey again. I was not refering to a tissue flotation bath. The Histobath was a product sold by Shandon. It had a chamber filled with isopentane or alcohol and was used for quick freezing tissue for a cryostat. Sorry if there was any confusion. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual mirotome
Our 23 year old Reichert Jung 2030s, with high profile blades. They are work horses Only one repair (the spring). Maintenance contract on a microtome? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.
I will be out of the office starting 07/12/2011 and will not return until 07/14/2011. Note: For Cytology issues, please call Molly at 8-421-5487, For Histology issues, please call the general histology lab 8-421- 5408. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
