[Histonet] RE: Assisting with Autopsies
We are a small hospital and though we no longer do in house autopsies we still had an autopsy assistant.(most prefer to be called this) We always had a pool of people available to do this job. Training as a histotech does not include this job and I have always refused to do it. I know there are techs who do not mind and some who supplement their income doing it but histotechs should not be forced to do them, certainly not free of charge. If enough histotechs in an area stand together and refuse the pathologists will find assistants. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Assisting with Autopsies
We have always helped with autopsies, just comes with the territory. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of susan.wal...@hcahealthcare.com Sent: Wednesday, July 20, 2011 3:32 AM To: as...@georgetownhospitalsystem.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Assisting with Autopsies We are a small hospital and though we no longer do in house autopsies we still had an autopsy assistant.(most prefer to be called this) We always had a pool of people available to do this job. Training as a histotech does not include this job and I have always refused to do it. I know there are techs who do not mind and some who supplement their income doing it but histotechs should not be forced to do them, certainly not free of charge. If enough histotechs in an area stand together and refuse the pathologists will find assistants. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Controls with patient specimen on same slide
We put patients and controls on the same slide. We used to use the slides with the red box for the control but encountered staining issues. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, July 19, 2011 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls with patient specimen on same slide Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Assisting with Autopsies
We do, on average, about 15 autopsies per year. We have a diener who does them for us. The pathologists are present and are often are hands on during the autopsy. Sometimes a lab aide will be there in the mode of a scribe. Our histotechs do not participate in autopsies. Michelle On Jul 20, 2011, at 6:06 AM, Podawiltz, Thomas tpodawi...@lrgh.org wrote: We have always helped with autopsies, just comes with the territory. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of susan.wal...@hcahealthcare.com Sent: Wednesday, July 20, 2011 3:32 AM To: as...@georgetownhospitalsystem.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Assisting with Autopsies We are a small hospital and though we no longer do in house autopsies we still had an autopsy assistant.(most prefer to be called this) We always had a pool of people available to do this job. Training as a histotech does not include this job and I have always refused to do it. I know there are techs who do not mind and some who supplement their income doing it but histotechs should not be forced to do them, certainly not free of charge. If enough histotechs in an area stand together and refuse the pathologists will find assistants. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Assisting with Autopsies
Here we all take turns doing the autopsies. We have call for weekends/holidays as well. We do have pathologist and residents in the suite with us, however we do the harvest and hand everything in one block from the trachea to the testicles to the docs to dissect and then we remove the brain and pituitary and place those if fixative for them. Clean up the body (put it back into the crypt) and then clean as much of the suite as possible while the docs are still dissecting the block. It is hard work and with the type of patient that we service it is not uncommon to have difficulties with extensive adhesions etc. I have had patients so large that I could hardly look into the cavity where I was trying to cut (needed a ladder). Years ago at a community hospital I was only an extra pair of hands, ahhh the good old days. You will need to be trained and it doesn't sound like your facility do enough to do this quickly, if they insist that HT/HTL be diener to assist then they need to have a good sign off procedure to train you properly so that there are no safety issues and give you time to practice. The other thing for us is we basically loose one tech for the day by the time they are finished and if you are like most labs and short staffed this can be an issue in getting the daily workload out on time. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax Date: Tue, 19 Jul 2011 15:12:06 -0400 From: Amy Self as...@georgetownhospitalsystem.org Subject: [Histonet] Assisting with Autopsies To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: eeed2ff216617d45aff64bf53ec4f9c712be04a...@gmhdtcexch.gmhpost.com Content-Type: text/plain; charset=us-ascii Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
It is the most effective and economical way to run your IHC's. We have been doing it that way for as long as I have been in histology. Thanks, Tim - Original Message - From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 19, 2011 6:04 PM Subject: Histonet Digest, Vol 92, Issue 25 Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
Just one. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 8:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
Each slide or else the whole theory of knowing each slide got treated the same goes out the window. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please
Re: [Histonet] Controls with patient specimen on same slide
We do put controls on each slide in a case. Sometimes it's just one slide that failed in a run. A batch control wouldn't tell you which slide failed if there are no internal controls in the patient tissue. I personally wouldn't feel comfortable doing IHC with batch controls. Mark On Wed, Jul 20, 2011 at 6:18 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Controls with patient specimen on same slide
In our Lab we put a control on every slide. We keep multiple controls cut and stored in refrigerator. Most are multi- tissue controls. We use the Ventana detection kits and it is not cost efficient to use 2 uses of the detection kit per antibody. Of course there are times that this will not work, then our cost per test goes up, but we cannot charge any more for a control. (Embedded image moved to file: pic32000.jpg)___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] please remove me from the list, thank you!
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RE: [Histonet] Leica Service Technician
Amanda, We have a Microm HM550 cryostat and have used Brian J. Hurley (New England Biomedical Services). He is an independent service engineer. He's very good. In fact ThermoFisher uses him if a service visit is needed. 781-331-8642 617-774-7368 (cell) Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cat Scratch control
Does anyone have an extra Cat Scratch control block to spare? We currently buy control sections from Newcomer, but I'm not convinced they are that good. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Service Technician
It is really just a freezer with a microtome in it. Do you have an on-site refrigeration guy that could look at it? I have used our in-house guy a time or two. Ours is pretty simple and does not have all of the electronics that you may have on the Leica but it is worth a shot. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
Each case gets one positive control per antibody. If, for example, I run a bone marrow bx and aspirate, I use one positive control for both slides. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Assisting with Autopsies
We have two contract deiners.. They work at other hospitals around town and charge per case. Works well here as our numbers have decreased drastically over the years. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 15:12 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Service Technician
Amanda, Im not exactly sure which part of your machine is freezing based on your description, but, I have a leica 1510 and it has freezing issues. Everyone I have ever used does this. The bar that hold the specimen chucks ices and freezes over like crazy.. It will frezze the chuck in the bar holder solid. I have to get a hammer and beat it sometimes..lol. So, before I use my machine I wipe 100% alcohol across the bar to de-ice it and the chucks dont get stuck..Do not use so much that you lower your temp. Also, do not get on the oct or the stage because your blocks will not cut and be mush... Just wipe the areas daily with alcohol.. Hope this helps. Nicole Tatum, HT ASCP Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica Service Technician
Hello Amanda, We are located in Eastern Pa and we use Belair Instruments out of NJ (908-518-2662). We have been very happy with their service on our cryostats. Donna L. Suresch Merck Research Laboratories Research Biologist Imaging Research - West Point -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, July 20, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 92, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Leica Service Technician (Amanda Madden) 2. Controls with patient specimen on same slide (shargr...@urhcs.org) 3. please remove me from the list, thank you! (Robert Cordero) 4. RE: Leica Service Technician (Sherwood, Margaret ) 5. Cat Scratch control (Houston, Ronald) 6. RE: Leica Service Technician (Tom McNemar) 7. RE: Controls with patient specimen on same slide (Tom McNemar) 8. RE: Assisting with Autopsies (Weems, Joyce) 9. Controls with patient specimen on same slide (Nancy Schmitt) -- Message: 1 Date: Wed, 20 Jul 2011 11:32:07 -0400 From: Amanda Madden amkmad...@gmail.com Subject: [Histonet] Leica Service Technician To: histonet@lists.utsouthwestern.edu Message-ID: CAC=+zgXtEK9=c0_nUSddHcSp4_7SDsn2jFxT3kYCJh=nswu...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda -- Message: 2 Date: Wed, 20 Jul 2011 10:35:33 -0500 From: shargr...@urhcs.org Subject: [Histonet] Controls with patient specimen on same slide To: histonet@lists.utsouthwestern.edu Message-ID: ofe0363896.5e031032-on862578d3.005508b0-862578d3.0055a...@urhcs.org Content-Type: text/plain; charset=us-ascii In our Lab we put a control on every slide. We keep multiple controls cut and stored in refrigerator. Most are multi- tissue controls. We use the Ventana detection kits and it is not cost efficient to use 2 uses of the detection kit per antibody. Of course there are times that this will not work, then our cost per test goes up, but we cannot charge any more for a control. (Embedded image moved to file: pic32000.jpg) -- Message: 3 Date: Wed, 20 Jul 2011 15:37:44 + From: Robert Cordero robert.cord...@comphealth.com Subject: [Histonet] please remove me from the list, thank you! To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 0ba36f96367e8a4cbb27e112faa6488a067...@vslcexmbp02.mychg.com Content-Type: text/plain; charset=us-ascii -- Message: 4 Date: Wed, 20 Jul 2011 11:43:46 -0400 From: Sherwood, Margaret msherw...@partners.org Subject: RE: [Histonet] Leica Service Technician To: Amanda Madden amkmad...@gmail.com, histonet@lists.utsouthwestern.edu Message-ID: 073ae2bea1c2ba4a8837ab6c4b943d9708db5...@phsxmb30.partners.org Content-Type: text/plain; charset=us-ascii Amanda, We have a Microm HM550 cryostat and have used Brian J. Hurley (New England Biomedical Services). He is an independent service engineer. He's very good. In fact ThermoFisher uses him if a service visit is needed. 781-331-8642 617-774-7368 (cell) Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From:
Re: [Histonet] Research microtomes
Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Research microtomes
Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] embalmers using formaldehyde - NYT story
This New York Times story about the use of formaldehyde by embalmers is worth reading by pathologists and histotechnologists. http://www.nytimes.com/2011/07/21/business/despite-cancer-risk-embalmers-stay-with-formaldehyde.html?_r=1hp Seems like they've basically got the right idea. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
