[Histonet] uncertainity of results in histopathology
Dear all please guide me about uncertainity of results in Histopathology at different steps,including fixation of tissue ,grossing,processing,microtomy,staining,IHC,special staining and interpretation etc. This is the requirement of ISO 15189 CLAUSE:5.6.2 Muhammad Tahseen SKMCHRC Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mouse vs. Human cells
Years ago, we used to distinguish human fibroblasts in mouse tissue with vimentin; which stains human cells quite nicely, but does not cross to mouse. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Carl Postenka cposte...@hotmail.com To: sdys...@mirnarx.com; histonet@lists.utsouthwestern.edu Sent: Tuesday, August 2, 2011 11:16 AM Subject: RE: [Histonet] Mouse vs. Human cells We've had succes identifying human cells in a mouse background using an antibody against human mitochondria (Neomarkers cat#MS-1372P). The primary is a monoclonal mouse anti-human, so we use a Dako ARK kit (#K3954) to eliminate the mouse on mouse background. HIER using citrate pH=6.0. H2O2 block. Primary diluted 1/100 (through ARK kit), for 20min @ R/T. -- Carl PostenkaLondon Regional Cancer Program Date: Mon, 1 Aug 2011 12:55:54 -0500 From: sdys...@mirnarx.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] Mouse vs. Human cells Hello Histo-hotties! Question: We are working with xenograft tumors in mice. The tumor cells are human in origin. I am trying to come up with some kind of stain that will stain all the mouse cells and none of the human cells. Just to be able to determine if any of the human tumor cells are in a normal looking say liver. I was thinking maybe Ki-67? It doesn't have to be any specific marker, just to be able to see a really blue mouse organ, and then if there are human cells of any type in the organ light up that one cell. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job opening
GI path lab in Santa Rosa, CA looking for a certified tech. Great opportunity!!! Great pay. Flexible hours. Friendly office. Submit resumes to tja...@yahoo.com. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hmm
Has anyone ever heard of the company, Stem cells? I have been trying to contact them all morning to order an antibody, but no one ever answers the phone. Little nervous. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC service ??
We've had excellent service from Ventana and they do have to travel from usually Chicago up to Madison. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Inman, Anna Sent: Mon 8/1/2011 11:30 AM To: histonet@lists.utsouthwestern.edu. Subject: [Histonet] IHC service ?? Can you tell me what kind of on-site technical service you are accustomed to receiving from Ventana (especially if you NOT in a big city so travel is required)? We have had a terrible time getting a Field service engineer into our lab this year and wonder if that is the norm for Ventana these days? For the Dako users - what kind of service are you getting? Thank you in advance Anna anna.in...@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cell blocks
How does everyone process their cell blocks? Currently, we use our biopsy run for smaller cell blocks, but the larger ones we use our routine tissue process. Our lab manager would like us to look at putting all cell blocks, regardless of size, on our biopsy run, to increase turn around time. I have my doubts, since some cell blocks, especially bronch washes and pleural fluids, can be quite large and greasy or mucous-y. What does everyone else do? We use standard 10% NBF, xylene, and paraplast xtra on our processors. We would process on a Leica Peloris. Our standard bx protocol is about 1.5 hours total, and our standard routine protocol is about 7 hours. Thank you! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOX-11
Hi: Has anyone had success with antibody SOX-11 and would be willing to share your protocol? Thanks in advance, -- *Marian L. Powers* *Q*uality *L*aboratory *C*onsultants *Doctors Pathology Services * ** c| 302.747.0580 o| 302.677. ext: 110 f | 302.677.0010 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cell blocks
I always processed cell blocks, regardless of their size, with the regular tissue processing protocol. René J. --- On Tue, 8/2/11, Clare Thornton cthorn...@dahlchase.com wrote: From: Clare Thornton cthorn...@dahlchase.com Subject: [Histonet] cell blocks To: 'Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwestern.edu Date: Tuesday, August 2, 2011, 1:14 PM How does everyone process their cell blocks? Currently, we use our biopsy run for smaller cell blocks, but the larger ones we use our routine tissue process. Our lab manager would like us to look at putting all cell blocks, regardless of size, on our biopsy run, to increase turn around time. I have my doubts, since some cell blocks, especially bronch washes and pleural fluids, can be quite large and greasy or mucous-y. What does everyone else do? We use standard 10% NBF, xylene, and paraplast xtra on our processors. We would process on a Leica Peloris. Our standard bx protocol is about 1.5 hours total, and our standard routine protocol is about 7 hours. Thank you! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cell blocks
you definitly can run them together. I would just test one before I put them all in there, because when they are small they can over process and be very hard and brittle making intrepretation difficult. As far as your larger fatty gooey specimens. Make sure they are no thicker than 3mm. Anything bigger doesnt process well on a normal 7hr cycle. Hope this helps Nicole Tatum HT ASCP How does everyone process their cell blocks? Currently, we use our biopsy run for smaller cell blocks, but the larger ones we use our routine tissue process. Our lab manager would like us to look at putting all cell blocks, regardless of size, on our biopsy run, to increase turn around time. I have my doubts, since some cell blocks, especially bronch washes and pleural fluids, can be quite large and greasy or mucous-y. What does everyone else do? We use standard 10% NBF, xylene, and paraplast xtra on our processors. We would process on a Leica Peloris. Our standard bx protocol is about 1.5 hours total, and our standard routine protocol is about 7 hours. Thank you! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cell blocks
We have a 6 hours program that we process ours on - our assistant works wee hours of the morning and has them ready to start around 6 am. If they don't get on same day, they go on the regular overnight run. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax From: Clare Thornton cthorn...@dahlchase.com Subject: [Histonet] cell blocks To: 'Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwestern.edu Date: Tuesday, August 2, 2011, 1:14 PM How does everyone process their cell blocks? Currently, we use our biopsy run for smaller cell blocks, but the larger ones we use our routine tissue process. Our lab manager would like us to look at putting all cell blocks, regardless of size, on our biopsy run, to increase turn around time. I have my doubts, since some cell blocks, especially bronch washes and pleural fluids, can be quite large and greasy or mucous-y. What does everyone else do? We use standard 10% NBF, xylene, and paraplast xtra on our processors. We would process on a Leica Peloris. Our standard bx protocol is about 1.5 hours total, and our standard routine protocol is about 7 hours. Thank you! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Perforin Ab
Can anyone recommend a perforin ab for use in FFPE human tissue? Thanks in advance Luis Luis Chiriboga Ph.D OCS Experimental Pathology IHC Core Lab Bellevue Hospital Center Department of Pathology 4w27 (212) 562-4667 luis.chirib...@nyumc.org /PRE html body br / This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.br / = /body /html PRE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Position in Springfield, Illinois
Histotechnician Job Description Basic Function: Process tissue and prepare slides for diagnosis by pathologists. Requirements for the position include: High school graduate required. HT(ASCP) registered or eligible Light physical effort. Job Location Springfield, IL US Position Type Full-Time/Regular Hours of Work 9:00 a.m. - 5:30 p.m. Weekends (specify) as needed Exempt or Non-Exempt Non-Exempt Our careers website is www.memorialcareers.comhttp://www.memorialcareers.com Breanne Kasprzyk│Recruitment Specialist │Memorial Health System │701 North First Street│ Springfield, IL 62781 •: 217.788.4587│7: 217.788.5539│•kasprzyk.brea...@mhsil.com •choosememorial.orghttp://www.memorialcareers.com/ €: Locationhttp://maps.google.com/maps?f=qsource=s_qhl=engeocode=q=200+West+Dodge+Street,+Springfield,+Illinois+62702sll=39.808668,-89.65569sspn=0.015396,0.03798ie=UTF8hq=hnear=200+W+Dodge+St,+Springfield,+Sangamon,+Illinois+62702ll=39.812146,-89.656141spn Thank you for choosing Memorial! James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] uncertainity of results in histopathology
Hi Muhammad I assume you are referring to Measurement of Uncertainty (MOU). This is only used for quantitative results and not applicable to Histology processing. The only times I have seen it applied in Anatomic Pathology are for Her2 and white cell counts on Broncho-alveolar lavages. EM staff use grids to make measurements on specimens and of course Pathologists use graticules to record measurements on tissue sections. Our local accreditation body has yet to make a call on these two measurements. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ tahs...@brain.net.pk 8/2/2011 9:39 pm Dear all please guide me about uncertainity of results in Histopathology at different steps,including fixation of tissue ,grossing,processing,microtomy,staining,IHC,special staining and interpretation etc. This is the requirement of ISO 15189 CLAUSE:5.6.2 Muhammad Tahseen SKMCHRC Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing of derm specimens
I have recently had a problem with my skin specimens being underprocessed. I use a Leica 300ASP. The schedule is as follows: 10% NBF x 2 for 1 hour ea. 80% Reagent Alcohol for 1 hour 95% Reagent Alcohol x 2 for 1 hour each 100% Reagent Alcohol for 1 hour each Xylene x 3 for 1 hour each Paraffin x 3 for 1 hour each The specimens are mushy and swell on the ice Any input is welcome. send response to : _scrochiere@nedlc.com_ (mailto:scrochi...@nedlc.com) thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processing of derm specimens
That processing schedule should be fine for skin samples, we add an additional 100% alcohol step so we have three absolute steps at 1 hour each (I would remove one 95%). Thickness of your samples is also important they should be around 3mm in thickness if they are thicker than that they may not process properly. The other thing is that your solutions need to be fresh for samples to process properly. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of crochierest...@aol.com Sent: Tuesday, August 02, 2011 4:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing of derm specimens I have recently had a problem with my skin specimens being underprocessed. I use a Leica 300ASP. The schedule is as follows: 10% NBF x 2 for 1 hour ea. 80% Reagent Alcohol for 1 hour 95% Reagent Alcohol x 2 for 1 hour each 100% Reagent Alcohol for 1 hour each Xylene x 3 for 1 hour each Paraffin x 3 for 1 hour each The specimens are mushy and swell on the ice Any input is welcome. send response to : _scrochiere@nedlc.com_ (mailto:scrochi...@nedlc.com) thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reorienting frozen tissue
Hi all, I would like to know if it is possible and how to reorient or reembedd human tissue, that has been frozen at -80C in OCT. Many thanks, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Manual embedding
Dear Histonetters: I am interested in acquiring a pitcher and heating jacket for melting and pouring paraffin during manual embedding. My work is relatively low volume and in a university research lab setting so I am trying to avoid purchasing an expensive embedding station. Can anyone recommend an honest supplier of used histology equipment that might be able to provide me with this item? Thank you for your expertise! Scott L. Parker ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
