RE: [Histonet] Problems with Frozen tissues
Good morning! I work with ovarie and mice uterus embebbed in oct. I get 8um frozen sections and put them un super frost slides, the I leave the slides in the fridge until use them. When I am going to perform the immunohistochemistry I leave the slides 1 minute at rt to atemperate the tissue, then I place the slides 10 minutes in acetone (stored at - 20ºC), whit this you fix the tissue and permeabilize it. with this protocol I never loss any sections. If you have any more question just write me. good luck, Carmen *** Carmen María García Pascual FIVI/INCLIVA/Facultad de Medicina Universidad de Valencia Departamento de P.O.G (Laboratorios) 96.386.40.48 Avd. Blasco Ibañez 17 46010, Valencia *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EBER ISH
Hi Sheila, Sorry for the delay. Yes, I am familiar with the EBER ISH probes on the Ventana Benchmark Ultra. Yes, the software that you will need to have installed is called U - ISH OPEN PROBES - CHROMOGENIC v3. This protocol will use the BLUE DETECTION and with a RED STAIN II counterstain. Before I get to the protocol, I'll fill you in on the probes. They do NOT arrive in a dispenser. They come in pre-diluted vials, and you will need to fill a user-fillable dispense. One more thing, you will need to run a control. This control will confirm the presence of non-degradated RNA. The control I used was U6, and the protocol is identical to EBER except for the selected ISH probe. By the way, the selected ISH probe will likely be ISH PROBE 1 or ISH PROBE 2 because the user-fillable dispenser will not have the proper commercial bar code. Finally, the following is a successful protocol summary (HybReady Soln is optional for high background): 1 Deparaffinization [Selected] 2 Warmup Slide to [69 Deg C], and Incubate for 16 Minutes ( Deparaffinization ) 3 Pretreatment [Selected] 4 CC2 [Selected] 5 CC2 Cycle 1 [Selected] 6 CC2 Cycle 2 [Selected] 7 Enzyme [Selected] 8 Apply One Drop of [ISH-PROTEASE 3] ( Enzyme ), and Incubate for [12 Minutes] 9 Probe [Selected] 10 Probe Auto Dispense [Selected] 11 Apply One Drop of [ISH Probe 5] ( ISH Probe ), No Coverslip and Incubate for 4 Minutes 12 Denature [Selected] 13 Warmup Slide to [75 Deg C], and Incubate for [4 Minutes] ( Denaturation ) 14 Hybridization [Selected] 15 Warmup Slide to [50 Deg C], and Incubate for 4 Minutes ( Hybridization ) 16 Incubate for [1 Hour] ( Hybridization ) 17 Stringency Washes [Selected] 18 Stringency Wash #1 [Selected] 19 Warmup Slide to [60 Deg C], and Incubate for [8 Minutes] ( Stringency Wash #1 ) 20 Stringency Wash #2 [Selected] 21 Incubate for [8 Minutes] ( Stringency Wash #2 ) 22 Stringency Wash #3 [Selected] 23 Incubate for [8 Minutes] ( Stringency Wash #3 ) 24 Detection Kit [Selected] 25 Blue Detection [Selected] 26 Incubate for [32 Minutes] ( Substrate ) 27 Counterstain [Selected] 28 Apply One Drop of [Red Stain II] ( Counterstain ), Apply Coverslip, and Incubate for [4 Minutes] Let me know if I forgot anything. Chris Lanigan Histonetters, Is anyone out there using the EBER ISH probes from Ventana on the Ultra Platform? I have a few questions. Did you need to get new software installed? Was there any training involved? And do you have successful protocols that you are willing to share? I have asked the questions before, but at the time there was nobody actually using it. I know that there were inquiries about the protocols because of the ASR status and the fact that Ventana cannot give you any advice. Any and all information would be greatly appreciated since we are wanting to start it up at our facility. Thanks, Sheila KDL Pathology Knoxville, TN Christopher Lanigan Research Technologist Molecular Pathology Cleveland Clinic Foundation 9500 Euclid Avenue L3-127 Cleveland, OH 44195 (216) 445-1443 === Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S.News World Report (2010). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
Perfect!! And I was afraid Halloween was going to sneak by unnoticed! Which vendor sells holy water, 'cause I'm gonna need some... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
Haven't seen it in their catalog, but you could try Erie Scientific ; ) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, November 02, 2011 9:23 AM To: O'Donnell, Bill; Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Perfect!! And I was afraid Halloween was going to sneak by unnoticed! Which vendor sells holy water, 'cause I'm gonna need some... ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
OOOH!!! Why didn't I think of that! Good goin'! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
You know sometimes I wish these emails had a LIKE button on them. I found this quite funny and wanted to LIKE it. Too much facebook for me! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: mast cells for arteries
Patsy, Just consider anti-tryptase antibody that is directly labeled with Alk. phos. (Millipore MAB1222A). Tris-EDTA pH9.0 pretreatment / 1:5000 overnight 4C / Vector Red. A tonsil will do as positive control. Good luck! Cheers, Chris Chris van der Loos Academic Medical Center Dept. of Pathology Amsterdam The Netherlands Date: Tue, 1 Nov 2011 11:07:47 -0600 From: Patsy Ruegg pru...@ihctech.net Subject: [Histonet] mast cells for arteries To: histonet@lists.utsouthwestern.edu Message-ID: 4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice Content-Type: text/plain; charset=us-ascii Does anyone have a go to stain for Mast Cells besides Tol Blue? We are trying to demonstrate the presence of mast cells in human arteries ffpe. Also, what would be a good positive control to use for mast cell staining, we used NH Skin and got maybe a few mast cells but I would like to use something more abundant with mast cells. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
At times like these, I wonder where Joe Nocito is... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
Yeah! We've really missed JTT! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, November 02, 2011 10:04 AM To: Beckham, Sharon; O'Donnell, Bill; Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC At times like these, I wonder where Joe Nocito is... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Where are you, JTT?
This being Halloween Week, you must be lurking out there somewhere... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Luciferase IHC
Julie, Are you looking to stain for firefly luciferase to confirm localization of an optical imaging probe? That's what I want to do, there are antibodies available from Lifespan and Biovision among others that are purported to work on FFPE sections I have yet to try them. You can check out this article, but they used a UK supplier. Bioluminescence imaging to monitor bladder cancer cell adhesion in vivo: a new approach to optimize a syngeneic, orthotopic, murine bladder cancer model. Jurczok A, Fornara P, Söling A. BJU Int. 2008 Jan;101(1):120-4. Epub 2007 Sep 20. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 . -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Tuesday, November 01, 2011 6:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] mast cells for arteries
Giemsa is what we use routinely for mast cell neoplasms and normal mast cells.
For us it works equally well as T blue for diagnostic purposes. Our positive
control is a cutaneous mast cell tumor (very common in out animal species), or
skin biopsy with allergic dermatitis.
Poorly granulated/degranulated mast cells won't stain with Giemsa or T Blue,
and in those cases we use c-Kit IHC
Best,
Sebastien
Sébastien Monette, DMV, MVSc, DACVP
Tri-Institutional Laboratory of Comparative Pathology
Center of Comparative Medicine and Pathology
Memorial Sloan-Kettering Cancer Center
Weill Cornell Medical College
The Rockefeller University
1275 York Avenue, Box 270, New York NY 10065
Phone: 646-888-2420
Fax: 646-422-0139
Email MSKCC: monet...@mskcc.org
Email WCMC: sem2...@med.cornell.edu
Web site: http://www.mskcc.org/mskcc/html/92361.cfm
-Original Message-
From: Patsy Ruegg [mailto:pru...@ihctech.net]
Sent: Tuesday, November 01, 2011 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] mast cells for arteries
Does anyone have a go to stain for Mast Cells besides Tol Blue? We are
trying to demonstrate the presence of mast cells in human arteries ffpe.
Also, what would be a good positive control to use for mast cell staining,
we used NH Skin and got maybe a few mast cells but I would like to use
something more abundant with mast cells.
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net
web site http://www.ihctech.net www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
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any dissemination, distribution, copying, or other use of this message, or
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[Histonet] Luciferase IHC!
Thanks for the help! We actually tried antigen retrieval in Holy water in a pressure cooker for 10 minutes. I even brought in an old priest and a young priest to perform the staining but no luck! Sorry, no vendor for the holy water - the old priest makes his own. I enjoyed the laugh! This project has been hell! Thanks, Julie Message: 21 Date: Wed, 2 Nov 2011 08:46:28 -0500 From: Beckham, Sharon s...@stowers.org Subject: RE: [Histonet] Luciferase IHC To: 'O'Donnell, Bill' billodonn...@catholichealth.net, Kim Donadio one_angel_sec...@yahoo.com, Randolph-Habecker, Julie jhabe...@fhcrc.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 2c40e43d1f7a56408c4463fd245dddf98aee2...@exchmb-02.stowers-institute.org Content-Type: text/plain; charset=iso-8859-1 You know sometimes I wish these emails had a LIKE button on them. I found this quite funny and wanted to LIKE it. Too much facebook for me! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 3 *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SC Society of Histotechnology this weekend
Hello to All, Just another reminder that our annual meeting of the SCSHT will be in Myrtle Beach, SC at the Marina Inn at Grande Dunes. Pre-registration is not required, you can register at the door! Good food, fun and fellowship and you will learn something too!!! Send me an email if you think you may be attending and have not registered. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CGRP immunopositive quantification
Hi, I ran ICC on CGRP and the stain is very specific, it's just messy, containing cell bodies as well as dense fibers. I can't really just do a cell count and I don't want to use densitometry. Does anybody have any suggestions on what methods to use for quantifying CGRP fibers? Any help would be great. Thanks, Rory. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Luciferase IHC!
This is great. I've enjoyed the story line and witty comments and remarks. Thanks,Nanne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Wednesday, November 02, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC! Thanks for the help! We actually tried antigen retrieval in Holy water in a pressure cooker for 10 minutes. I even brought in an old priest and a young priest to perform the staining but no luck! Sorry, no vendor for the holy water - the old priest makes his own. I enjoyed the laugh! This project has been hell! Thanks, Julie Message: 21 Date: Wed, 2 Nov 2011 08:46:28 -0500 From: Beckham, Sharon s...@stowers.org Subject: RE: [Histonet] Luciferase IHC To: 'O'Donnell, Bill' billodonn...@catholichealth.net, Kim Donadio one_angel_sec...@yahoo.com, Randolph-Habecker, Julie jhabe...@fhcrc.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 2c40e43d1f7a56408c4463fd245dddf98aee2...@exchmb-02.stowers-institute.org Content-Type: text/plain; charset=iso-8859-1 You know sometimes I wish these emails had a LIKE button on them. I found this quite funny and wanted to LIKE it. Too much facebook for me! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 3 *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Luciferase IHC/Holy water
You can make your own holy water. You put regular water on a stove and boil the hell out of it! Mike Titford USA - Pathology Mobile AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
And it's not even Friday! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kim Donadio Sent: Tue 11/1/2011 7:00 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC/Holy water
Very similar to how I cook kidneys. (ever notice why there are no great Irish restaurants or cookbooks in the U.S.? William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Wednesday, November 02, 2011 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC/Holy water You can make your own holy water. You put regular water on a stove and boil the hell out of it! Mike Titford USA - Pathology Mobile AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] out of office at noon today
I will be out of the office starting 11/02/2011 and will not return until 11/07/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. I will be in town. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] deparaffinization with detergent
I have seen a few comments over the years that mention deparaffinization of slides using hot soapy water instead of xylene. Does anyone have a reference they can direct me to where I can learn more about this or personal experience on how well it works and the logistics of making it work in the real world? Stephanie Weaver Texas Veterinary Medical Diagnostic Laboratory ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FULL TIME CERTIFIED HISTOTECH WANTED
New GI lab opening in Santa Monica Calif. If you are looking to advance in your career to the next level and challenge yourself to new technology, than this might just be the perfect opportunity for you. Some specifics are: Lead Tech position, full time / 1st shift available, but flexible on the hours. Must be self motivated and work well independently. Knowledge of QA/QC a plus, Grossing involved. Position available immediately. Please submit resumes to e-mail below. Any questions you can call at the contact information below. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 909-841-9761. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mast cells for arteries
If you don't want the with purple-red metachromasia, try neutral red, which
gives an orange-red metachromasia and true red nuclei.
John Kiernan
Anatomy, UWO
London, Canada
= = =
On 01/11/11, Patsy Ruegg pru...@ihctech.net wrote:
Does anyone have a go to stain for Mast Cells besides Tol Blue? We are
trying to demonstrate the presence of mast cells in human arteries ffpe.
Also, what would be a good positive control to use for mast cell staining,
we used NH Skin and got maybe a few mast cells but I would like to use
something more abundant with mast cells.
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net pru...@ihctech.net
web site http://www.ihctech.net(http://www.ihctech.net/) www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
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Re: [Histonet] mast cells for arteries
Breast fibroadenoma is a great mast cell control
Rosie Scrimizzi
Oz
Sent from my iPad
On 03/11/2011, at 1:27 AM, monet...@mskcc.org wrote:
Giemsa is what we use routinely for mast cell neoplasms and normal mast
cells. For us it works equally well as T blue for diagnostic purposes. Our
positive control is a cutaneous mast cell tumor (very common in out animal
species), or skin biopsy with allergic dermatitis.
Poorly granulated/degranulated mast cells won't stain with Giemsa or T Blue,
and in those cases we use c-Kit IHC
Best,
Sebastien
Sébastien Monette, DMV, MVSc, DACVP
Tri-Institutional Laboratory of Comparative Pathology
Center of Comparative Medicine and Pathology
Memorial Sloan-Kettering Cancer Center
Weill Cornell Medical College
The Rockefeller University
1275 York Avenue, Box 270, New York NY 10065
Phone: 646-888-2420
Fax: 646-422-0139
Email MSKCC: monet...@mskcc.org
Email WCMC: sem2...@med.cornell.edu
Web site: http://www.mskcc.org/mskcc/html/92361.cfm
-Original Message-
From: Patsy Ruegg [mailto:pru...@ihctech.net]
Sent: Tuesday, November 01, 2011 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] mast cells for arteries
Does anyone have a go to stain for Mast Cells besides Tol Blue? We are
trying to demonstrate the presence of mast cells in human arteries ffpe.
Also, what would be a good positive control to use for mast cell staining,
we used NH Skin and got maybe a few mast cells but I would like to use
something more abundant with mast cells.
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net
web site http://www.ihctech.net www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
=
Please note that this e-mail and any files transmitted with it may be
privileged, confidential, and protected from disclosure under
applicable law. If the reader of this message is not the intended
recipient, or an employee or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
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Re: [Histonet] deparaffinization with detergent
See Buesa Peshkov 2009 Annals of Diagnostic Pathology 13: 246-256. They thoroughly describe and discuss the method. John Kiernan Anatomy, UWO London, Canada = = = On 02/11/11, Weaver, Stephanie swea...@tvmdl.tamu.edu wrote: I have seen a few comments over the years that mention deparaffinization of slides using hot soapy water instead of xylene. Does anyone have a reference they can direct me to where I can learn more about this or personal experience on how well it works and the logistics of making it work in the real world? Stephanie Weaver Texas Veterinary Medical Diagnostic Laboratory ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] DAB Immuno and alcian blue counter staining
Try decreasing the alcian blue concentration and staining time. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniela Bodemer Sent: Tuesday, November 01, 2011 8:47 PM To: Histonet@lists.utsouthwestern.edu Cc: Susan Lapthorne Subject: [Histonet] DAB Immuno and alcian blue counter staining Hi all, Is it possible to do an immuno with DAB detection and counterstain it with alcian blue at the end to detect Goblets cells? I've tried it and the DAB disappears under the staining. Thanks, Daniela __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Must be Monday...
Yea what is up with that all of a sudden my Dako autostainer is an hour behind, it must have switched to the dst when we used to do that and now we do that later, right, so I will have to wait a while for it to catch up, when does that happen now days? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 6:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Must be Monday...
Next Sunday my dear.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, November 02, 2011 17:05 To: 'Breeden, Sara'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Must be Monday... Yea what is up with that all of a sudden my Dako autostainer is an hour behind, it must have switched to the dst when we used to do that and now we do that later, right, so I will have to wait a while for it to catch up, when does that happen now days? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 6:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] General orientation checklist
Does anyone have a general orientation checklist for new hires that they would be willing to share . Thanks in advance Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Must be Monday...
Yep I have an older Dako and I forget that it changes to dst at the old time, well the new change should come up this weekend Nov 6th so everything should be back on schedule after that? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, October 31, 2011 6:40 AM To: 'Breeden, Sara'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Must be Monday... That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] problem with brain sections falling off subbed slides
Hello, I am new to working with brain, and I am having a bad problem with both thin and thick rat brain sections sliding off of subbed slides. We tried commercial treated slides first, but then moved on to subbing them. The subbing was done with gelatin according to a printed protocol, and I can see the film on the dried slide. When kept horizontal they seem okay, but as soon as they are put into a slide holder for washing or anything, they begin to slide off or even move around. After a short while, many/most of mysteriously vanished (into the wash solution). Our slices are sliced by cryostat from a rat brain that has been fixed with 4% paraformaldehyde and 30% sucrose. The slice comes right off the cryostat, and then I m ove it onto a warm slide. (Perhaps they are not warm enough?? I have seen others blow on them to warm them.) Slides destined for Cresyl violet staining were dried for 3 days at room temperature before being moved. It is the Cresyl violet protocol that causes the most trouble, but I am seeing some problems with any type of large volume wash. At this point, I don't have enough experience with this specific tissue and our protocols to be able to tell for sure what is happening. Thus, I am entirely open to advice - any advice that might help with the problem. Thank you-Doug Burns, Kansas City ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Luciferase IHC!
I'm here. I was asking my Priest the steps to perform an exorcism, although the Catholic Church doesn't perform these rituals any longer. I have all the materials, readings and exorcism paraphernalia Just let me know where and when. Just make sure there is a good bottle of Scotch available, just in case all else fails. JTT - Original Message - From: Marsh, Nannette n...@stowers.org To: 'Randolph-Habecker, Julie' jhabe...@fhcrc.org; histonet@lists.utsouthwestern.edu Sent: Wednesday, November 02, 2011 11:31 AM Subject: [Histonet] RE: Luciferase IHC! This is great. I've enjoyed the story line and witty comments and remarks. Thanks,Nanne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Wednesday, November 02, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC! Thanks for the help! We actually tried antigen retrieval in Holy water in a pressure cooker for 10 minutes. I even brought in an old priest and a young priest to perform the staining but no luck! Sorry, no vendor for the holy water - the old priest makes his own. I enjoyed the laugh! This project has been hell! Thanks, Julie Message: 21 Date: Wed, 2 Nov 2011 08:46:28 -0500 From: Beckham, Sharon s...@stowers.org Subject: RE: [Histonet] Luciferase IHC To: 'O'Donnell, Bill' billodonn...@catholichealth.net, Kim Donadio one_angel_sec...@yahoo.com, Randolph-Habecker, Julie jhabe...@fhcrc.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 2c40e43d1f7a56408c4463fd245dddf98aee2...@exchmb-02.stowers-institute.org Content-Type: text/plain; charset=iso-8859-1 You know sometimes I wish these emails had a LIKE button on them. I found this quite funny and wanted to LIKE it. Too much facebook for me! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 3 *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Where are you, JTT?
My son is heavily in high school activities now. I have to drive him to all these school events. I know I'll probably pay for this later down the road, but I'm actually pushing him to get his driver's permit so he can drive. by time we get home, it's time for my beauty sleep. If you saw me in Cinci, you'd understand that I need all the help I can get. JTT - Original Message - From: Breeden, Sara sbree...@nmda.nmsu.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 02, 2011 9:08 AM Subject: [Histonet] Where are you, JTT? This being Halloween Week, you must be lurking out there somewhere... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.
I will be out of the office starting 11/02/2011 and will not return until 11/10/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology issues, please call Mario at 8-421-4961, general histology lab 8-421- 5408, Kiran at 8-421-5404, or Wanda at 8-421-5426. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Luciferase IHC!
a good exorcism is hard to come by. Good scotch, I think I can provide. Ive looked and looked and the cure or diagnosis for this is hard to find. All the nuns agreed till de-cloaking was mentioned :D From: Joe Nocito jnoc...@satx.rr.com To: Marsh, Nannette n...@stowers.org; 'Randolph-Habecker, Julie' jhabe...@fhcrc.org; histonet@lists.utsouthwestern.edu Sent: Wednesday, November 2, 2011 7:26 PM Subject: Re: [Histonet] RE: Luciferase IHC! I'm here. I was asking my Priest the steps to perform an exorcism, although the Catholic Church doesn't perform these rituals any longer. I have all the materials, readings and exorcism paraphernalia Just let me know where and when. Just make sure there is a good bottle of Scotch available, just in case all else fails. JTT - Original Message - From: Marsh, Nannette n...@stowers.org To: 'Randolph-Habecker, Julie' jhabe...@fhcrc.org; histonet@lists.utsouthwestern.edu Sent: Wednesday, November 02, 2011 11:31 AM Subject: [Histonet] RE: Luciferase IHC! This is great. I've enjoyed the story line and witty comments and remarks. Thanks,Nanne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Wednesday, November 02, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC! Thanks for the help! We actually tried antigen retrieval in Holy water in a pressure cooker for 10 minutes. I even brought in an old priest and a young priest to perform the staining but no luck! Sorry, no vendor for the holy water - the old priest makes his own. I enjoyed the laugh! This project has been hell! Thanks, Julie Message: 21 Date: Wed, 2 Nov 2011 08:46:28 -0500 From: Beckham, Sharon s...@stowers.org Subject: RE: [Histonet] Luciferase IHC To: 'O'Donnell, Bill' billodonn...@catholichealth.net, Kim Donadio one_angel_sec...@yahoo.com, Randolph-Habecker, Julie jhabe...@fhcrc.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 2c40e43d1f7a56408c4463fd245dddf98aee2...@exchmb-02.stowers-institute.org Content-Type: text/plain; charset=iso-8859-1 You know sometimes I wish these emails had a LIKE button on them. I found this quite funny and wanted to LIKE it. Too much facebook for me! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 3 *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
Re: [Histonet] problem with brain sections falling off subbed slides
Gelatin alone is not an adhesive because it dissolves in water. Chrome alum-gelatin is an excellent adhesive for sections and costs almost nothing to make. Subbed slides are one coated with chrome gelatin, dried and stored. The following web link shows a 1999 publication giving the rationale and methods for a variety of section adhesives. http://publish.uwo.ca/~jkiernan/adhesivs.htm John Kiernan Anatomy,UWO London, Canada = = = On 02/11/11, Douglas M Burns dmbur...@gmail.com wrote: Hello, I am new to working with brain, and I am having a bad problem with both thin and thick rat brain sections sliding off of subbed slides. We tried commercial treated slides first, but then moved on to subbing them. The subbing was done with gelatin according to a printed protocol, and I can see the film on the dried slide. When kept horizontal they seem okay, but as soon as they are put into a slide holder for washing or anything, they begin to slide off or even move around. After a short while, many/most of mysteriously vanished (into the wash solution). Our slices are sliced by cryostat from a rat brain that has been fixed with 4% paraformaldehyde and 30% sucrose. The slice comes right off the cryostat, and then I m ove it onto a warm slide. (Perhaps they are not warm enough?? I have seen others blow on them to warm them.) Slides destined for Cresyl violet staining were dried for 3 days at room temperature before being moved. It is the Cresyl violet protocol that causes the most trouble, but I am seeing some problems with any type of large volume wash. At this point, I don't have enough experience with this specific tissue and our protocols to be able to tell for sure what is happening. Thus, I am entirely open to advice - any advice that might help with the problem. Thank you-Doug Burns, Kansas City ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
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