[Histonet] RE: Source of IgG4 for Isotype control
Cell Marque 0.1 ml concentrated 367M-14 0.5 ml concentrated 367M-15 1 ml concentrated 367M-16 1 ml prediluted 367M-17 7 ml prediluted 367M-18 We use it routinely and it works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ladd, Sharron [sharron.l...@immunogen.com] Sent: Thursday, November 17, 2011 4:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Source of IgG4 for Isotype control Hi Histonet, Where can I buy IgG4 in the US please? Thanks, Sharron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Source of IgG4 for Isotype control
Sorry I should have been more specific. I am not looking for a mouse monoclonal anti-human IgG4 antibody. I need human IgG4 to run as an isotype control for another primary mouse monoclonal that is an IgG4 isotype. The only company I have found so far that sell purified Human IgG4 is in England and the shipping time is too long. Happy Friday! Sharron -Original Message- From: McMahon, Loralee A [mailto:loralee_mcma...@urmc.rochester.edu] Sent: Friday, November 18, 2011 8:15 AM To: Ladd, Sharron; 'histonet@lists.utsouthwestern.edu' Subject: RE: Source of IgG4 for Isotype control Cell Marque 0.1 ml concentrated 367M-14 0.5 ml concentrated 367M-15 1 ml concentrated 367M-16 1 ml prediluted 367M-17 7 ml prediluted 367M-18 We use it routinely and it works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ladd, Sharron [sharron.l...@immunogen.com] Sent: Thursday, November 17, 2011 4:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Source of IgG4 for Isotype control Hi Histonet, Where can I buy IgG4 in the US please? Thanks, Sharron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microtomes
Hi, If you are concerned about the thickness of the sections being accurate to the setting, I would suggest picking up a cheap micrometer from a hardware store. (OK perhaps not cheap as you will want a quality one, but the cost of these isn't terrible.) You can't really measure 5 microns (or whatever you cut at) well, so you will need to take 10-50 sections of a blank block. Remember to keep a constant rhythm that you would use on a normal block. Measure the thickness before and after then divide these thicknesses by the number of sections taken. Remember, also, to take the measurements at the same temperature that you are cutting the sections at. If your difference isn't exactly right how far off is it? You would then know if you should use a higher or lower setting on the microtome. Amos On Thu, Nov 17, 2011 at 10:33 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Message: 13 Date: Thu, 17 Nov 2011 16:02:50 -0600 From: Salomao Segal sseg...@slu.edu Subject: [Histonet] microtomes To: histonet@lists.utsouthwestern.edu Message-ID: cacdnk8huzek7dgaept--kjzy219ynwgmepiwq5dkorpjtn8...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 The settings in a rotary microtome may indicate the thickness of sections but... how do you know that it indeed cuts at the indicated thickness, particularly if it is say an old device that you inherit from somebody else's lab junk? Is there a way of measuring the magnitude of advances after each rotation? Thanks Solomon Segal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Source of IgG4 for Isotype control
First try Sigma Aldrich William DeSalvo, B.S., HTL(ASCP) From: sharron.l...@immunogen.com To: loralee_mcma...@urmc.rochester.edu; histonet@lists.utsouthwestern.edu Date: Fri, 18 Nov 2011 08:22:05 -0500 CC: Subject: [Histonet] RE: Source of IgG4 for Isotype control Sorry I should have been more specific. I am not looking for a mouse monoclonal anti-human IgG4 antibody. I need human IgG4 to run as an isotype control for another primary mouse monoclonal that is an IgG4 isotype. The only company I have found so far that sell purified Human IgG4 is in England and the shipping time is too long. Happy Friday! Sharron -Original Message- From: McMahon, Loralee A [mailto:loralee_mcma...@urmc.rochester.edu] Sent: Friday, November 18, 2011 8:15 AM To: Ladd, Sharron; 'histonet@lists.utsouthwestern.edu' Subject: RE: Source of IgG4 for Isotype control Cell Marque 0.1 ml concentrated 367M-14 0.5 ml concentrated 367M-15 1 ml concentrated 367M-16 1 ml prediluted 367M-17 7 ml prediluted 367M-18 We use it routinely and it works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ladd, Sharron [sharron.l...@immunogen.com] Sent: Thursday, November 17, 2011 4:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Source of IgG4 for Isotype control Hi Histonet, Where can I buy IgG4 in the US please? Thanks, Sharron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antibodies for hamster tissue
Hi all , Wondering if anyone has experience with these antibodies on hamster tissues: - Cathepsin B or a pan-cathepsin Ab if on exists - MMP-3, MMP9, MMP12 - neutrophil elastase Thanks for any info. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Great Position in AZ
Great opportunity for a Histotechnician in Scottsdale , AZ . Colon and Digestive Health Specialists is looking for a certified HT or HTL to run their laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours up to 25 hours a week . Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@carisls.com http://webmail.windstreamhosting.com/hwebmail/services/go.php?url=http% 3A%2F%2Fmailto%3Amhale%40carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Great Position in Louisiana
Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@carisls.com http://webmail.windstreamhosting.com/hwebmail/services/go.php?url=http% 3A%2F%2Fmailto%3Amhale%40carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mouse xGFAP on rabbit
Good Morning, Does anyone know of a *mouse* xGFAP that will work on rabbit tissue? Thanks in advance, -- Jan Shivers Senior Scientist IHC/Histology/EM Section Head Pathology Teaching Program UMN Veterinary Diagnostic Laboratory University of Minnesota College of Veterinary Medicine 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Great Position in AZ
Great opportunity for a Histotechnician in Scottsdale , AZ . Colon and Digestive Health Specialists is looking for a certified HT or HTL to run their laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours up to 25 hours a week . Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@carisls.com mailto:mh...@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problems in processing rat paws
Hello everyone! I have a problem in processing rat paws to paraffin embedding. I need to process and cut the whole paw (except fingers). So, first I need to decalcify the paw and then process it to paraffin embedding. My problem is that when I try to cut the paw I can not get a good section because the tissue have a gum-like consistence (mushy). Do you know where is my problem? Dehydration, clearing, embedding? ** *Note*: I decalcify the paws in formic acid and aluminum chloride for 24h; Then I wash samples in phosphate buffer saline for 5 hours. After that I did the following manual processing (applying vacuum): 2x30min in 70% ethanol, 2x30min in 80% ethanol, 2x30min in 96% ethanol, 2x30min in absolute ethanol, 3x15 min in Xilene, 2x15min in paraffin and a final incubation of 30min in paraffin. Then, I proceed to paraffin ambedding and try to cut sections of 5um of thickness. If anyone can help me I will be grateful. Thanks in advance, Cátia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problems in processing rat paws
Catia Its going to take about a week to decal those types of samples in formic acid and in addition to that the processing cycle needs to be considerably longer. By rat paw, I'm assuming you are mean the front paw or is it the rear ankle that you need to process. First of all the samples need to be fixed for at least 48 hours prior to decalcification. We use 10% formic acid in distilled water, seems to work fine for us. For ankle samples without the rear toes, it takes about a week to decal. If the toes are attached in our hands it takes about two weeks, we change the decal about every third day or so and always on the day prior to processing. Ankles are trimmed in half and processed, both halves in one cassette. For ankle and toes, we trim one side of the joint off prior to processing. For front paws since they are a bit thick I would trim off some of the wrist pad prior to processing. Processing cycle is long. 1 hour in each station for ankles and 1 to 2 hours per station for ankles with toes. We use three absolutes and three xylenes and 4 paraffins when processing. 70% - 1 hour 80% - 1 hour 95% - 1 hour 100% - 1 hour 100% - 1 hour 100% - 1 hour Xylene - 1 hour Xylene - 1 hour Xylene - 1 hour Paraffin - 1 hour Paraffin - 1 hour Paraffin - 1 hour Good luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Catia Lopes Sent: Friday, November 18, 2011 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems in processing rat paws Hello everyone! I have a problem in processing rat paws to paraffin embedding. I need to process and cut the whole paw (except fingers). So, first I need to decalcify the paw and then process it to paraffin embedding. My problem is that when I try to cut the paw I can not get a good section because the tissue have a gum-like consistence (mushy). Do you know where is my problem? Dehydration, clearing, embedding? ** *Note*: I decalcify the paws in formic acid and aluminum chloride for 24h; Then I wash samples in phosphate buffer saline for 5 hours. After that I did the following manual processing (applying vacuum): 2x30min in 70% ethanol, 2x30min in 80% ethanol, 2x30min in 96% ethanol, 2x30min in absolute ethanol, 3x15 min in Xilene, 2x15min in paraffin and a final incubation of 30min in paraffin. Then, I proceed to paraffin ambedding and try to cut sections of 5um of thickness. If anyone can help me I will be grateful. Thanks in advance, Cátia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] clearing of whole tissue
Hi, anyone has experience in tissue clearing? i want to clear spinal cord after fixation. Thanks. YJ -- Truth Shall Make You Free ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Hot Histology Job Alert - Histology Tech needed in Virginia. Can you help?
Hi Histonetters!! I hope you are gearing up for a fun Pre-Thanksgiving weekend. I have a great job opportunity to tell you about. I am currently working with one of my best clients and they are in need of a histotech. I refer to them as one of my best clients because they have very low turnover and I have only placed people with them due to growth. This position is for their lab in Roanoke, VA. This is a full time day shift M-F position. My client offers a great compensation package and an awesome team to work with. If you are ASCP HT/HTL or eligible, have at least 2-3 years of routine histology experience (IHC is a plus) and would like to live in beautiful Roanoke, VA then my client wants to talk to you. Whether you would like to start a new position RIGHT AWAY or would prefer to start a new position AFTER the holidays the choice is up to you. My client is very flexible. If you would like more information please contact me. I can be reached toll free at 866-607-3542 or via email at rel...@earthlink.net. If you know someone else that might be interested please feel free to pass my information along. If you refer someone to me and I place them you will receive a referral bonus. (That sure could come in handy with the holidays around the corner) :). Thanks for taking the time to read this post and Enjoy Your Weekend. Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtomes
Do you have access to a confocal microscope or one that does Z-stack imaging? You can find out the thickness of your tissue sections that way (after you've mounted them on a slide of course). Regards, Merced -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, November 18, 2011 8:55 AM To: histonet@lists.utsouthwestern.edu; sseg...@slu.edu Subject: [Histonet] microtomes Hi, If you are concerned about the thickness of the sections being accurate to the setting, I would suggest picking up a cheap micrometer from a hardware store. (OK perhaps not cheap as you will want a quality one, but the cost of these isn't terrible.) You can't really measure 5 microns (or whatever you cut at) well, so you will need to take 10-50 sections of a blank block. Remember to keep a constant rhythm that you would use on a normal block. Measure the thickness before and after then divide these thicknesses by the number of sections taken. Remember, also, to take the measurements at the same temperature that you are cutting the sections at. If your difference isn't exactly right how far off is it? You would then know if you should use a higher or lower setting on the microtome. Amos On Thu, Nov 17, 2011 at 10:33 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Message: 13 Date: Thu, 17 Nov 2011 16:02:50 -0600 From: Salomao Segal sseg...@slu.edu Subject: [Histonet] microtomes To: histonet@lists.utsouthwestern.edu Message-ID: cacdnk8huzek7dgaept--kjzy219ynwgmepiwq5dkorpjtn8...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 The settings in a rotary microtome may indicate the thickness of sections but... how do you know that it indeed cuts at the indicated thickness, particularly if it is say an old device that you inherit from somebody else's lab junk? Is there a way of measuring the magnitude of advances after each rotation? Thanks Solomon Segal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] clearing spinal cord
YJ, You would probably be interested in this: Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Hiroshi Hama et al., Nature Neuroscience 14, 1481–1488 (2011) --- Message: 1 Date: Fri, 18 Nov 2011 09:17:36 -0800 From: aget liang agetli...@gmail.com Subject: [Histonet] clearing of whole tissue To: histonet@lists.utsouthwestern.edu Message-ID: Hi, anyone has experience in tissue clearing? i want to clear spinal cord after fixation. Thanks. YJ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: To cut or not to cut mouse brains
I have had folding and bubbles occur periodically with PFA fixed, sucrose cryoprotected frozen brain tissue I receive from other labs. I streak a distilled water moistened brush across the slide then pick up the frozen section on the moistened area. It comes out flat and smooth every time. I let the section dry as usual, then store in -20°C or -80°C. For ISH I use a RNase zapped and DEPC rinsed brush and streak DEPC water across the slide with the moistened brush. I section the PFA fixed, sucrose cryoprotected tissue around -21°C. Researchers report good subsequent IHC and ISH results and bring more samples. Donna J. Emge, ASCP-HT Mouse Histology and Phenotyping Laboratory Manager Northwestern University Olson Pavilion 8-333 710 North Fairbanks Court Chicago, IL 60611 d-e...@northwestern.edumailto:d-e...@northwestern.edu 312-503-2679 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PT Testing
How are those of you in Technical Component labs handling your PT testing ? And if you are grading these slides how are you setting the criteria and what are you looking at ? Thanks Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraplast X-tra
Hey Connie, Remember that we experienced a drastic heat wave all over the country and in some parts there were rolling blackouts. The product may have been stored in a warehouse that experienced a rolling blackout and the increased temperature messed up the paraffin which then re-hardened and was sold.The bag looked fine, so nobody thought anything was wrong. Stranger things have happened. The overheating would cause the oily and muddy. I say this because a few years back we had a problem with coverslips that were stuck together. Even though they were new and still sealed, when opened they were stuck. It turned out there was excess moisture in the warehouse and the silica gel didn't work. We returned them and got replacements. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 15 Date: Thu, 17 Nov 2011 15:46:16 -0800 From: connie grubaugh conniegruba...@hotmail.com Subject: RE: [Histonet] Paraplast X-tra To: one_angel_sec...@yahoo.com, cls71...@sbcglobal.net, histonet@lists.utsouthwestern.edu Message-ID: blu153-w4295022d3e87dd9a19cf80d6...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 We are having problems again too. Have not changed a thing and convinced that it is a bad lot again. Wonder sometimes on the QC that is done on the paraffin. Connie G. Date: Wed, 16 Nov 2011 13:47:28 -0800 From: one_angel_sec...@yahoo.com To: cls71...@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast X-tra CC: Maybe you got a bad lot that is contaminated causing the knife marks? Also, Ive noticed that if you let your paraffin get to hot, it breaks down and that could be one reason for a consistency issue. Have you changed anything at all recently to your process? Hope this was helpful. Kim From: Cristi stephenson cls71...@sbcglobal.net To: Histo Net histonet@lists.utsouthwestern.edu Sent: Wednesday, November 16, 2011 2:06 PM Subject: [Histonet] Paraplast X-tra Hello Histoland, We are seeing unusually large volumes of fall out in our paraffin lately. The precipitate is almost muddy or oily in appearance. We noticed it immediately as it was melting, so there was no way anything else had been introduced to cause this. As a result, we are seeing knife marks. The marks are not consistent and not attributable to any of the tissue in the blocks. We are blasting through blades that have been tried and true for years now. Are there any other users of paraplast X-tra experiencing these issues? Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 16 Date: Thu, 17 Nov 2011 16:08:33 -0800 From: cls71...@sbcglobal.net cls71...@sbcglobal.net Subject: Re: [Histonet] Paraplast X-tra To: connie grubaugh conniegruba...@hotmail.com, one_angel_sec...@yahoo.com, histonet@lists.utsouthwestern.edu Message-ID: 74764.79442...@smtp214.mail.bf1.yahoo.com Content-Type: text/plain; charset=utf-8 I had to believe someone else out there was seeing this too!! Our rep contacted us today and is going to replace it and investigate...I too wonder how this gets past the QC dept., it is super obvious as soon as it starts melting Sent from my HTC on the Now Network from Sprint! - Reply message - From: connie grubaugh conniegruba...@hotmail.com Date: Thu, Nov 17, 2011 3:46 pm Subject: [Histonet] Paraplast X-tra To: one_angel_sec...@yahoo.com, cls71...@sbcglobal.net, histonet@lists.utsouthwestern.edu -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: clearing of whole tissue
Well, I have used all of the usual suspects over many years but, this one has taken me by complete surprise! If anyone can explain the rationale of the reaction, I would be most interested. One for J Kiernan, I think? Ah, yes: Sca/e: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain Fascinating! I love the forward slash before the ehow should it be pronounced? Is it related to Wall-e ( Many thanks for the info, Squash buddy Phil ;-) Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fwd: IHC control
-Marian Begin forwarded message: From: Marian Powers mpow...@dpspa.com Date: November 16, 2011 12:51:56 PM EST To: histonet@lists.utsouthwestern.edu Subject: IHC control Hi: Would anyone have a hereditary nonpolyposis colorectal cancer control, (for MHL1, MSH2, MSH6) to share? Thanks in advance, -- Marian L. Powers Doctors Pathology Services c| 302.747.0580 o| 302.677. ext: 110 f | 302.677.0010 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: clearing of whole tissue
Did anyone know what is the buffer system for the 4M urea? it is important to adjust and maintain pH to 7.7 according to the paper: Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain 2011/11/18 aget liang agetli...@gmail.com Hi, anyone has experience in tissue clearing? i want to clear spinal cord after fixation. Thanks. YJ -- Truth Shall Make You Free -- Truth Shall Make You Free ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
