RE: [Histonet] Does xylene cause skin cancer?
I would be worried too. Wearing gloves in the lab is always a good practice and I believe, a requirement in my lab whenever handling reagents or the possibility of coming into contact with reagents. You have the right to wear gloves if you want. Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Tuesday, February 21, 2012 10:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does xylene cause skin cancer? I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Storage of Frozen Tissues
How does everyone store tissues that are at -20 or -80? Currently we wrap the tissue well in foil and place in a labeled cassette. If shipped out, we double bag as required. Recently, a concern has been raised about the cassettes being a safety issue due to the fact that the tissue could be exposed to staff. Any ideas? Thanks, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6168 Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at complia...@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. --- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Does xylene cause skin cancer?
There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. René J. --- On Tue, 2/21/12, Jenny Vega histotech...@gmail.com wrote: From: Jenny Vega histotech...@gmail.com Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Does xylene cause skin cancer?
If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. Debbie Faichney Institute of Aquaculture University of Stirling UK -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 22 February 2012 13:09 To: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. René J. --- On Tue, 2/21/12, Jenny Vega histotech...@gmail.com wrote: From: Jenny Vega histotech...@gmail.com Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Storage of Frozen Tissues
Tissues in my tumor bank (-80ºC) were kept in small (1inchx2inches) labeled zip-lock bags. I do not understand about your concern regarding tissues being exposed to staff. Inside the bags I used, the tissues were not exposed. If they were taken out for any study or procedures, universal precautions were taken. René J. --- On Wed, 2/22/12, Dennis Hahn dennis.h...@cookchildrens.org wrote: From: Dennis Hahn dennis.h...@cookchildrens.org Subject: [Histonet] Storage of Frozen Tissues To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Wednesday, February 22, 2012, 7:20 AM How does everyone store tissues that are at -20 or -80? Currently we wrap the tissue well in foil and place in a labeled cassette. If shipped out, we double bag as required. Recently, a concern has been raised about the cassettes being a safety issue due to the fact that the tissue could be exposed to staff. Any ideas? Thanks, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6168 Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at complia...@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. --- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cytology CSF Cell Pellets made from Histogel
I work in a research lab, but I use histogel all the time to make FFPE blocks of cell culture material. Here is my procedure: 1. you need about 5X10^ cells per pellet 2. spin cells @ 2000 rpm for 5 minutes in a 50 ml conical tube 3. aspirate and resuspend in 15ml NBF and fix overnight @ RT 4. spin @ 2000 rpm for 5 minutes and aspirate the NBF 5. microwave the histogel (thermo #HG-4000-012) for 15-20 seconds (loosen cap before microwaving) 6. histogel should now be at liquid for and about 60C 7. resuspend cell pellet in about 350ul of histogel 8. place cell pellet in freezer or on ice for about 20 minutes to solidify 9. using spatula, remove histogel pellet and place into cassette 10. post-fix in NBF for 2 hours (this step is very important); histogel will dissolve in processor if not fixed 11. process as usual Good luck! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Turner, Leandra lturn...@seton.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, February 21, 2012 12:26 PM Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel Hello Everyone, I am trying to find out a few of things about making cell pellets on cerebrospinal fluids. I would like to know: 1. If anyone has ever made cell pellets from CSF's using Histogel and has any tips or procedures they could share? 2. How to process the CSF pellets made with Histogel, do we need a routine or stat process? (we use a Sakura Tissue Tek) 3. Can you do IHC staining on the pellets? Thank you for any and all help in advance. Leandra Turner, HT (ASPC)CM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cytology CSF Cell Pellets made from Histogel
I forgot to mention, we prepare these specifically for IHC; so yes, you can do IHC on them. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Kim Merriam kmerriam2...@yahoo.com To: Turner, Leandra lturn...@seton.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, February 22, 2012 8:21 AM Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel I work in a research lab, but I use histogel all the time to make FFPE blocks of cell culture material. Here is my procedure: 1. you need about 5X10^ cells per pellet 2. spin cells @ 2000 rpm for 5 minutes in a 50 ml conical tube 3. aspirate and resuspend in 15ml NBF and fix overnight @ RT 4. spin @ 2000 rpm for 5 minutes and aspirate the NBF 5. microwave the histogel (thermo #HG-4000-012) for 15-20 seconds (loosen cap before microwaving) 6. histogel should now be at liquid for and about 60C 7. resuspend cell pellet in about 350ul of histogel 8. place cell pellet in freezer or on ice for about 20 minutes to solidify 9. using spatula, remove histogel pellet and place into cassette 10. post-fix in NBF for 2 hours (this step is very important); histogel will dissolve in processor if not fixed 11. process as usual Good luck! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Turner, Leandra lturn...@seton.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, February 21, 2012 12:26 PM Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel Hello Everyone, I am trying to find out a few of things about making cell pellets on cerebrospinal fluids. I would like to know: 1. If anyone has ever made cell pellets from CSF's using Histogel and has any tips or procedures they could share? 2. How to process the CSF pellets made with Histogel, do we need a routine or stat process? (we use a Sakura Tissue Tek) 3. Can you do IHC staining on the pellets? Thank you for any and all help in advance. Leandra Turner, HT (ASPC)CM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Does xylene cause skin cancer?
You might want to go to something with even greater chemical resistance - thin nitrile is not recommended for use with xylene: http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx According to our xylene MSDS: The substance may be toxic to blood, kidneys, liver, mucous membranes, bone marrow, central nervous system (CNS). Repeated or prolonged exposure to the substance can produce target organs damage. I would definitely use gloves if I were you. Hilary -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debbie Faichney Sent: Wednesday, February 22, 2012 8:15 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] Does xylene cause skin cancer? If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. Debbie Faichney Institute of Aquaculture University of Stirling UK -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 22 February 2012 13:09 To: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. René J. --- On Tue, 2/21/12, Jenny Vega histotech...@gmail.com wrote: From: Jenny Vega histotech...@gmail.com Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Histology Supervisor Job in Naples, FL
Allied Search Partners has been retained for the following searches in Florida. Please forward this along to anyone who you know that would be interested in any of the following positions. We do offer a referral bonus. Please email a copy of updated resume to meli...@alliedsearchpartners.com mailto:bran...@alliedsearchpartners.com for a full job description. We have the following positions available: Histotech LOCATION: Naples, FL DEPARTMENT SCHEDULE: Monday-Friday Day Shift/Full Time (6:30am-2:30pm) Histology Supervisor LOCATION: Naples, FL DEPARTMENT SCHEDULE: *Bachelor¹s Degree Required, FL Histology Supervisor License Eligible Monday-Friday Day Shift/Full Time -- Melissa Phelan, President Laboratory Staffing Allied Search Partners http://www.linkedin.com/in/melissaphelan http://www.linkedin.com/in/melissaphelan P: 888-388-7571 F: 888-388-7572 C: 407-697-1175 www.alliedsearchpartners.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temp verifier slides - Ventana equipment
Do y'all run the temp verifier slides for the quarterly maintenance for the Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 months, esp if you have multiple pieces of equipment. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Does xylene cause skin cancer?
Nitrile gloves are recommended for all the chemicals we use in our lab except acetone - for acetone latex is recommended. We also coverslip by hand but we wear nitrile gloves without exception. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Tuesday, February 21, 2012 8:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does xylene cause skin cancer? I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] questions
For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc?? How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved forever in case tissue is needed for a molecular test and there is not enough left in the block. Appreciate ahead of time your responses! Dorothy Webb, HT (ASCP) Regions Hospital This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Does xylene cause skin cancer?
I don't know of any evidence that xylene causes skin cancer. Concern is with absorption through the skin. The most likely problem is with the bone marrow - leukemia and related diseases - from aromatic hydrocarbons (xylene, toluene, benzene) - which of course are present in resinous mounting media even in xylene free laboratories. Latex gloves dissolve rapidly. Nitrile rubber is more resistant, though not very. I don't know about vinyl examination gloves. I don't wear gloves in this situation, but obviously a pathologist gets much less exposure than a histotechnologist does. I certainly wouldn't argue with anyone who wanted to wear them. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Does xylene cause skin cancer?
Hi Jenny: Xylene can pass through the skin via the lipids that surround the skin cells, just the way the medicine in nicotine patches, sea sickness patches and birth control patches can. How much can enter your system is not known to me but you should wear resistant gloves, maybe even double glove. Not wearing the appropriate glove is almost certainly a safety violation. Geoff On 2/21/2012 10:17 PM, Jenny Vega wrote: I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seeking a Histology Supervisor in Connecticut
Slone Partners seeks a Histology Supervisor for our hospital based laboratory client in Connecticut. The successful candidate will have knowledge and ability to operate microtomes manual and automated; operate tissue processors, automated stainer, coverslipper, slide/cassette write; to embed tissues of various sizes and orientations; and to perform special stains both manual and automated. Must be a team player, responsible, proactive, goal and people oriented, focused, punctual and open to learning new tasks. Qualified candidates will have either HT/HTL certification, with supervisory experience, preferably in a busy, high-volume laboratory. Special features of this position: The histology supervisor will have5 years experience in a clinical laboratory with emphasis in anatomic pathology/histology. If you meet these qualifications and would like to be considered for this position, please submit your resume to Darcy Bloch at dar...@slonepartners.com. If you have experience in the diagnostic laboratory industry and wish to be considered for other roles, please forward your resume to Tara Kochis at t...@slonepartners.com. All inquiries are kept confidential. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Does xylene cause skin cancer?
We use nitrile to coverslip at our lab. Several of us including myself have latex allergy as well as the latex breaks down quickly. Most labs require you to use gloves handling any chemical substance. I have one tech who has years of experience that will suffer greatly at 1 drop of xylene or xylene substitute. With one drop the skin will be eaten away for quite a long time. So nitrile all the way! I don't know about xylene causing skin cancer but heard an old wives tale once that a HT had gotten liver cancer from exposure to xylene. Who knows if it is true? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thank You to all histology professionals!
Histotechnology Professionals Day is coming up soon! March 10, 2012 Histotechnology Professionals Day is an event designed to bring public awareness and recognition to histotechnology practitioners around the world. It offers an opportunity to recognize and appreciate the individuals who have dedicated themselves to quality patient care. Thank You to all histology professionals! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 http://us.mc538.mail.yahoo.com/mc/compose?to=br...@prometheushealthcare.com br...@prometheushealthcare.com http://www.prometheushealthcare.com/ www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog http://twitter.com/PrometheusBlog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: Subject: Re: [Histonet] Storage of Frozen Tissues
Hi, We freeze store muscle bx's brain tissue at -70°C since the neuro lab was started (around 1980). We started using cryogenic containers after we found that over time the tissue would dry out with any other type of storage we have tried them all. Hope this helps, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sal...@dsmanitoba.ca This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Does xylene cause skin cancer?
Debbie: Are the 30 min official? Any standard procedure that states 30 min as maximum? If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. Debbie Faichney Institute of Aquaculture University of Stirling UK -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 22 February 2012 13:09 To: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. René J. --- On Tue, 2/21/12, Jenny Vegahistotech...@gmail.com wrote: From: Jenny Vegahistotech...@gmail.com Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: questions
Hi Dorothy, I have used reagent alcohol for years both with staining and tissue processing. I have never noticed any artifacts or crystals due to use of reagent alcohol. One thing that you should know is that all denatured alcohols are not the same so make sure that you buy a denatured (reagent) alcohol of good quality. As far as keeping slides unstained forever, the one thing that you need to take into consideration is that if the slide is charged or silane coated that the positive charges (adhesiveness) of the slide will change over time and the sections may fall off during staining. Also the antigenicity of some antibodies decreases over time so if you were to do IHC staining, your results may not be as strong as they would be on a more recently cut slide. Best wishes, Debbie Siena HT (ASCP) QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsi...@statlab.com | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, February 22, 2012 12:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc?? How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved forever in case tissue is needed for a molecular test and there is not enough left in the block. Appreciate ahead of time your responses! Dorothy Webb, HT (ASCP) Regions Hospital This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] questions
Dorothy, saving cut slides forever is not a good idea in my opinion. They attract dust, fungus,Bacteria, all kinds of artifacts on top of loosing antigen specificity. Cut slides is not the answer to me. I had to deal with this a couple if times hopefully your pathologist will understand it's best to leave the cells in the block till need. Now I will say on certain protocols we would take sections and stain them but really need to get out of habit of cutting a bunch of extras that even if you did need would be sub optimal material because of what we just spoke of. Keep it in the block as much as you can. Just my two cents. Kim D Sent from my iPhonem On Feb 22, 2012, at 1:18 PM, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc?? How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved forever in case tissue is needed for a molecular test and there is not enough left in the block. Appreciate ahead of time your responses! Dorothy Webb, HT (ASCP) Regions Hospital This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] questions
Hi Dorothy If you want to prolong the viability of pre-cut unstained slides, firstly dry them, then dunk the slide into a bath of molten paraffin wax deep enough to submerge all of the tissue section, then remove it and allow the wax coating to solidify. This will keep the environment out of your cut section. When you need to use the slides, simply heat to melt the excess wax and then dewax and rehydrate in the usual manner. Regards, Mark Bromley. Manager, Histopathology. ___ Melbourne Pathology. 103 Victoria Pde, Melbourne. | (03) 92877806 www.mps.com.au -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, 23 February 2012 5:18 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc?? How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved forever in case tissue is needed for a molecular test and there is not enough left in the block. Appreciate ahead of time your responses! Dorothy Webb, HT (ASCP) Regions Hospital This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Does xylene cause skin cancer?
Hi all, My 2 cents worth, just be careful which gloves you wear. The reason for latex residues appearing on the back of the slides is that you are wearing the wrong gloves. Nitrile gloves are what you need to be using, latex will dissolve in the xylene. Remember (1): Disposable gloves provide a barrier protection when working with small amounts of laboratory chemicals. If a disposable glove becomes contaminated, remove immediately and replace with a new glove. Never reuse disposable gloves. (1) Guidelines for the selection of Chemically Resistant Gloves (http://www.bioc.cam.ac.uk/safety/glove_guide.pdf) (2) Ansell Chemical Resistance Guide (http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf) http://www.aps.anl.gov/Safety_and_Training/User_Safety/gloveselection.html Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Wednesday, 22 February 2012 10:47 PM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Does xylene cause skin cancer? I would be worried too. Wearing gloves in the lab is always a good practice and I believe, a requirement in my lab whenever handling reagents or the possibility of coming into contact with reagents. You have the right to wear gloves if you want. Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Tuesday, February 21, 2012 10:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does xylene cause skin cancer? I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Does xylene cause skin cancer?
Wow, That's interesting; KIMBERLY-CLARK Nitrile Gloves are not resistant to xylene, whereas Ansell's Nitrile gloves are (see http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilary Smith Sent: Thursday, 23 February 2012 1:52 AM To: Debbie Faichney; Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] Does xylene cause skin cancer? You might want to go to something with even greater chemical resistance - thin nitrile is not recommended for use with xylene: http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx According to our xylene MSDS: The substance may be toxic to blood, kidneys, liver, mucous membranes, bone marrow, central nervous system (CNS). Repeated or prolonged exposure to the substance can produce target organs damage. I would definitely use gloves if I were you. Hilary -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debbie Faichney Sent: Wednesday, February 22, 2012 8:15 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] Does xylene cause skin cancer? If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. Debbie Faichney Institute of Aquaculture University of Stirling UK -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 22 February 2012 13:09 To: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. René J. --- On Tue, 2/21/12, Jenny Vega histotech...@gmail.com wrote: From: Jenny Vega histotech...@gmail.com Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list
[Histonet] Re: Histonet Digest, Vol 99, Issue 28
Message: 15 Date: Wed, 22 Feb 2012 15:26:43 + From: Amber McKenzie amber.mcken...@gastrodocs.net Subject: [Histonet] Temp verifier slides - Ventana equipment To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 5a33c952bb67f4468af1f36d739212bc115ec...@jerry.gia.com Content-Type: text/plain; charset=us-ascii Do y'all run the temp verifier slides for the quarterly maintenance for the Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 months, esp if you have multiple pieces of equipment. Amber, We used regular Superfrost Plus slide (ie. vWR Fisher) they work just fine. Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX Histology) madelein...@elcaminohospital.org On Wed, Feb 22, 2012 at 10:01 AM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Seeking a Histology Supervisor in Connecticut (Darcy Bloch) 2. RE: Processor Question (Davide Costanzo) 3. Job Opening in Orange county California (Paula Lucas) 4. Cytology CSF Cell Pellets made from Histogel (Amos Brooks) 5. Does xylene cause skin cancer? (Jenny Vega) 6. RE: Does xylene cause skin cancer? (Settembre, Dana) 7. Storage of Frozen Tissues (Dennis Hahn) 8. Re: Does xylene cause skin cancer? (Rene J Buesa) 9. RE: Does xylene cause skin cancer? (Debbie Faichney) 10. Re: Storage of Frozen Tissues (Rene J Buesa) 11. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) 12. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) 13. RE: Does xylene cause skin cancer? (Hilary Smith) 14. Histotech Histology Supervisor Job in Naples, FL (Melissa Phelan) 15. Temp verifier slides - Ventana equipment (Amber McKenzie) 16. RE: Does xylene cause skin cancer? (Goins, Tresa) -- Message: 1 Date: 21 Feb 2012 13:08:28 -0500 From: Darcy Bloch dar...@slonepartners.com Subject: [Histonet] Seeking a Histology Supervisor in Connecticut To: histonet@lists.utsouthwestern.edu Message-ID: mailman.0.1329933601.18415.histo...@lists.utsouthwestern.edu Content-Type: text/plain; charset=us-ascii Slone Partners seeks a Histology Supervisor for our hospital based laboratory The successful candidate redesign and be great at managing change. people in the department, including 2 supervisors. experience is a plus. Qualified certification, with high-volume laboratory. Special features of this position: have the opportunity to help redesign this If you meet these qualifications for this position, please submit your resume dar...@slonepartners.com. If you wish to be considered Tara Kochis at t...@slonepartners.com. All inquiries are kept confidential. -- Message: 2 Date: Tue, 21 Feb 2012 10:33:27 -0800 From: Davide Costanzo pathloc...@gmail.com Subject: RE: [Histonet] Processor Question To: Gauch, Vicki gau...@mail.amc.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: -2296715788321707800@unknownmsgid Content-Type: text/plain; charset=ISO-8859-1 Design flaw in the screen display. It is in the way of the chamber when opening chamber. If your not careful you will break the screen. Happens fairly often. Sent from my Windows Phone From: Gauch, Vicki Sent: 2/21/2012 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor Question Hi everyone, We are in the market for new processors...and I was wondering if anyone could give me some pros and cons for the Tissue Tek VIP 6 tissue processor - how reliable are they? Ease of use ? Any known problems? Tissues process well? You knowall the usual questions we all ask for new equipment. Thanks in advance for your help, Vicki Gauch AMCH Albany, NY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Tue, 21 Feb 2012 12:23:07 -0800 From: Paula Lucas plu...@biopath.org Subject: [Histonet] Job Opening in Orange county California To: histonet@lists.utsouthwestern.edu Message-ID: B4B63414CF0349E7A008F0FD4B17E64C@biopath.local Content-Type: text/plain; charset=US-ASCII A part-time histotech
