[Histonet] Sunquest CoPath Listserver?

[Watkins David]

Does anyone know if there is a Sunquest CoPath listserv?
We are upgrading to v.6, and I  would like to mine it for information  
about this new version.


thx,

David Watkins, MD
Department of Pathology
Baylor University Medical Center


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RE: [Histonet] Shiny side of a paraffin section

[Tony Henwood (SCHN)]

Consistency, Consistency, Consistency.

1.  It is easier to place sections on the water bath with the shiny surface 
down (MOST times).
2.  Staining-wise, for all stains that I have tried, it does not matter 
(H&E, PAS, Perl's Trichrome to name a few).
3.  When you are matching blocks and slides prior to slides leaving the 
lab, it is difficult to match "rebelliously collected shiny-side up sections" 
with the corresponding blocks.
4.  It is a favourite test of mine to have my staff and trainees look at 
two slides and tell me the reason for the difference (if there is any). My 
staff call it "Tony's Migraine Quiz" - one 'properly' collected section and the 
second a "rebelliously collected shiny-side up section".
4.  Microscopically, when comparing special stains with a H&E, it can drive 
you to distraction when microscopic features do not line up.


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey
Sent: Wednesday, 29 February 2012 10:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Shiny side of a paraffin section

As all of us who cut paraffin know, the underside of each section as it comes 
off the blade is shiny. I've always accepted it as a fact that the shiny side 
always goes down on the water bath, but I've begun to wonder why. Is there a 
specific reason why we're all taught to put the shiny side down? What would the 
difference be between a 'properly' collected section and a rebelliously 
collected shiny-side up section? Does it even matter?

Thanks!
Lucie

Lucie Guernsey
UC San Diego
lguern...@ucsd.edu
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RE: [Histonet] Shiny side of a paraffin section

[Tony Henwood (SCHN)]

Who wants to look at Cell bottoms all day!!

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Hoy
Sent: Wednesday, 29 February 2012 12:36 PM
To: Histonet
Subject: Re: [Histonet] Shiny side of a paraffin section

All of the cells would be face down when you looked at them!

(It's already been a long week!)

Eric Hoy

===
Eric S. Hoy, Ph.D., SI(ASCP)
Clinical Associate Professor
Department of Medical Laboratory Sciences The University of Texas Southwestern 
Medical Center Dallas, Texas
Email: eric@utsouthwestern.edu
===


On 2/28/12 5:56 PM, "Lucie Guernsey"  wrote:

> As all of us who cut paraffin know, the underside of each section as 
> it comes off the blade is shiny. I've always accepted it as a fact 
> that the shiny side always goes down on the water bath, but I've begun 
> to wonder why. Is there a specific reason why we're all taught to put 
> the shiny side down? What would the difference be between a 'properly' 
> collected section and a rebelliously collected shiny-side up section? Does it 
> even matter?
> 
> Thanks!
> Lucie
> 
> Lucie Guernsey
> UC San Diego
> lguern...@ucsd.edu
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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RE: [Histonet] autofluorescence and sudan black

[Leiker, Merced]

It should not interfere. I use Sudan Black a lot. What concentration do you use?

Regards,
Merced


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia 
Pascual [carstj2...@hotmail.com]
Sent: Wednesday, February 29, 2012 7:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] autofluorescence and sudan black

Good morning!I am Carmen from the University of Valencia. Now I am performing
immunofluorescence of mouse decidua. I was having problems with the
autofluorescence so I found a sudan black protocol and I probed it, I
incubated the tissue 30 min with sudan black after the staining, but I
lostes the red signal (alexa 594) so I repited the experiment but
incubating before the immunohistochemestry, and want to know if you
know if this migth interfere with the antibody attachment to the tissue.thank 
you very much!kind regards!Carmen   
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[Histonet] Cryostat

[Rolly Perez]

Anyone interested in a used, working Cryostat, contact me directly:

Leica/Reichert-Jung CRYOCUT 1800

Rolly Perez, HT(ASCP)
Histology Supervisor
MedSurg Pathology Associates, Inc.
Tigard, OR
503.443.2157




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[Histonet] AUTO: Mielnikowski, Diane is out of the office. (returning 03/01/2012)

[Diane . Mielnikowski]

I am out of the office until 03/01/2012.

I am out at  a customer site  all day and without access to email or
voicemail.  I will return your message as soon as possible.  If this is an
emergency, please text my cell at 847-257-3111.  Diane


Note: This is an automated response to your message  "Histonet Digest, Vol
99, Issue 37" sent on 2/29/2012 11:59:17 AM.

This is the only notification you will receive while this person is away.


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[Histonet] RE: Secret Identity

[Morken, Timothy]

Sally, it was easy - aren't you the only histotech in New Mexico?

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Wednesday, February 29, 2012 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Secret Identity

It just occurred to me (after prompting from a friend on Histonet who knows my 
Secret Identity) that you might not know who nmhisto@comcast is.  That would be 
me.  I'm transitioning to my home base and get Histonet there.  That explains 
the strange posting from that person, does it not?  But, yes, I'm still working 
- for now.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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[Histonet] Secret Identity

[Breeden, Sara]

It just occurred to me (after prompting from a friend on Histonet who
knows my Secret Identity) that you might not know who nmhisto@comcast
is.  That would be me.  I'm transitioning to my home base and get
Histonet there.  That explains the strange posting from that person,
does it not?  But, yes, I'm still working - for now.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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[Histonet] Histotechnologist Needed in Fort Myers, FL

[Brannon Owens]

Allied Search Partners is currently looking for a qualified applicant for a
Histotechnologist for a position available in a Fort Myers, FL laboratory.
 
Position: Histotechnologist
Schedule:  Full time/permanent
 
Summary: 
 
Perform a variety of routine and specialized histology techniques and
procedures. 
Embedding, Microtomy, Grossing, Processing, and H&E staining
Special Staining
Equipment maintenance
 
Requirements:
 
FL Laboratory License
Previous experience with automated IHC
Technical and QC protocols
AA or BS/BA degree in life science
 
Benefits:
 
Competitive salaries, Health, Dental, Life & Disability insurances, a
section 125 plan, a 401K, FSA, ESPP and relocation assistance.
 
To Apply:
 
Please send resume to bran...@alliedsearchpartners.com

 

-- 
*If you wish to no longer receive emails from Allied Search Partners please
respond to this email message with "remove."
 
Brannon Owens, Recruitment Manager
LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823

Allied Search Partners

T: 888.388.7571 ext. 106

F: 888.388.7572

www.alliedsearchpartners.com 

Tell us about your experience with ASP by clicking on this link:
http://ratepoint.com/tellus/82388  

 

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[Histonet] dickeyer

[Rene J Buesa]

Learn how to become successfuI from home
http://www.countrydiscountgrocery.com/servalicy.php?cybytheme=42


Wed, 29 Feb 2012 18:12:50

" The castle of Lord Clifford was built at the opening of the gorge, and it 
commanded an enchanting view of the valley below" (c) cole ainslie

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Re: [Histonet] CAP approved shelf life of dyes & solutions

[Rene J Buesa]

Such a list does not exist because the shelf life of every reagent (either dry 
or in solution) is determined by the manufacturer depending on their own 
specifications or desired turn-over buying/replenishing rate.
René J.

--- On Wed, 2/29/12, Sharon Allen  wrote:


From: Sharon Allen 
Subject: [Histonet] CAP approved shelf life of dyes & solutions
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, February 29, 2012, 11:20 AM


Hi everyone,

Does anyone have a list of the shelf life of solutions, chemicals etc.
used in Histo staining methods that is acceptable for CAP accreditation
& would share the information?

We are trying to get a list together but different sources often
conflict. I would appreciate any information.

Thanks

Sharon Allen

Senior Medical Technologist

Neuropathology Lab-MS435U

Health Sciences Centre

820 Sherbrook Street

Winnipeg,MB, CA 

R3A 1R9

e-mail: sal...@dsmanitoba.ca




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[Histonet] CAP approved shelf life of dyes & solutions

[Sharon Allen]

Hi everyone,

Does anyone have a list of the shelf life of solutions, chemicals etc.
used in Histo staining methods that is acceptable for CAP accreditation
& would share the information?

We are trying to get a list together but different sources often
conflict. I would appreciate any information.

Thanks

Sharon Allen

Senior Medical Technologist

Neuropathology Lab-MS435U

Health Sciences Centre

820 Sherbrook Street

Winnipeg,MB, CA 

R3A 1R9

e-mail: sal...@dsmanitoba.ca

 

This email and/or any documents in this transmission is intended for the
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information.  Any unauthorized use, disclosure, distribution, copying or 
dissemination is strictly prohibited.  If you receive this transmission in 
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Ce courriel et tout document dans cette transmission est destiné à la personne 
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RE: [Histonet] Shiny side of a paraffin section

[Smith, Allen]

My mentor, Nick Roman, told me that sections adhere to the slide better if they 
go on shiny side down.  Brenda Disbrey's HISTOLOGICAL LABORATORY METHODS says 
that laying the sections on the water bath or water droplet shiny side down 
makes it easier to remove creases.  Benno Romeiss' MIKROSKOPISCHE TECHNIK and 
Manfred Gabe's TECHNIQUES HISTOLOGIQUES say that section should be mounted 
shiny side down without giving a reason. Most other authors do not even mention 
this matter.

Allen A. Smith
Professor of Anatomy
Barry University School of Podiatric Medicine
Miami Shores, Florida

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey
Sent: Tuesday, February 28, 2012 6:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Shiny side of a paraffin section

As all of us who cut paraffin know, the underside of each section as it
comes off the blade is shiny. I've always accepted it as a fact that the
shiny side always goes down on the water bath, but I've begun to wonder
why. Is there a specific reason why we're all taught to put the shiny side
down? What would the difference be between a 'properly' collected section
and a rebelliously collected shiny-side up section? Does it even matter?

Thanks!
Lucie

Lucie Guernsey
UC San Diego
lguern...@ucsd.edu
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Re: [Histonet] Shiny side of a paraffin section

[Rene J Buesa]

Placing the shiny-side of the section on the water surface you assure that the 
sections corresponds to the block, of course you cannot turn-around the 
section. Also it will allow water tension to expand the section better and 
assures a better adhesion to the slide surface.
If the section is in a ribbon you will have to decide which in the ribbon to 
select and you should not turn around the section. Orientation should not be an 
issue unless you are always going to section the block in the same way if 
recuts are needed.
René J.

--- On Tue, 2/28/12, Lucie Guernsey  wrote:


From: Lucie Guernsey 
Subject: [Histonet] Shiny side of a paraffin section
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, February 28, 2012, 6:56 PM


As all of us who cut paraffin know, the underside of each section as it
comes off the blade is shiny. I've always accepted it as a fact that the
shiny side always goes down on the water bath, but I've begun to wonder
why. Is there a specific reason why we're all taught to put the shiny side
down? What would the difference be between a 'properly' collected section
and a rebelliously collected shiny-side up section? Does it even matter?

Thanks!
Lucie

Lucie Guernsey
UC San Diego
lguern...@ucsd.edu
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Re: [Histonet] anti-GFP staining rabbit polyclonal A11122

[Emily Sours]

The heating I was referring to was the 60C the paraffin must be melted at
to embed the tissue.  We also use a vaccuum oven to paraffin embed so maybe
that also damages the antigen.  We usually leave tissue up to six hours in
the paraffin oven before embedding it at room temperature.

Emily

The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson



On Tue, Feb 28, 2012 at 9:14 AM, Paula Pierce <
cont...@excaliburpathology.com> wrote:

> Antigen retrieval will not hurt the GFP antigen in formalin fixed tissue.
>
> Paula K. Pierce, HTL(ASCP)HT
> President
> Excalibur Pathology, Inc.
> 8901 S. Santa Fe, Suite G
> Oklahoma City, OK 73139
> 405-759-3953 Lab
> 405-759-7513 Fax
> www.excaliburpathology.com
>
>   *From:* Emily Sours 
> *To:* Marina Pils ;
> histonet@lists.utsouthwestern.edu; Paula Pierce <
> cont...@excaliburpathology.com>
> *Sent:* Tuesday, February 28, 2012 8:08 AM
> *Subject:* Re: [Histonet] anti-GFP staining rabbit polyclonal A11122
>
> We have never been able to get this antibody working with paraffin
> embedding on mice tissue.  It works fine with frozen sections however.  We
> always figured it was the heating that destroyed the GFP antigen.  Paula,
> what fixation protocol and dilution do you use?
>
> Emily
>
>
>
> The whole point of this country is if you want to eat garbage, balloon up
> to 600 pounds and die of a heart attack at 43, you can! You are free to do
> so. To me, that’s beautiful.
> --Ron Swanson
>
>
>
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[Histonet] autofluorescence and sudan black

[Carmen Maria Garcia Pascual]

Good morning!I am Carmen from the University of Valencia. Now I am performing 
immunofluorescence of mouse decidua. I was having problems with the 
autofluorescence so I found a sudan black protocol and I probed it, I 
incubated the tissue 30 min with sudan black after the staining, but I 
lostes the red signal (alexa 594) so I repited the experiment but 
incubating before the immunohistochemestry, and want to know if you 
know if this migth interfere with the antibody attachment to the tissue.thank 
you very much!kind regards!Carmen   
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RE: [Histonet] Cardiac Myocytes

[Leiker, Merced]

We use Cardiac Troponin I or Cardiac Troponin T all the time (pigs).  These are 
also in mice. 

Regards,
Merced


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks 
[amosbro...@gmail.com]
Sent: Tuesday, February 28, 2012 3:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cardiac Myocytes

Hi,
What would one use to identify cardiac myocytes in mice. I know there
are specific antibodies, but I was kinda hoping for something a bit more
mundane like desmin.

Any ideas?
Amos
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RE: [Histonet] Agar for cell blocks

[Tom McNemar]

We use soy agar slants that we get from our Micro department.  We just melt one 
as needed.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo
Sent: Tuesday, February 28, 2012 5:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Agar for cell blocks


Is anyone using an agar for their cell block preparation that they can 
recommend?  ann
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[Histonet] Shiny Side of Paraffin Section

[nmhisto]

Okay, I can't stand this!  Although I  always make sure my ribbon is placed 
shiny-side-down on the water bath, sometimes the only section this poor ol' 
tech is able to get might land upside down.  In that case, I just tell the 
pathologist the turn the slide over on the 'scope.  Kinda like when they give 
me a piece of tissue that has to be stuffed into the base mold?  I have to wrap 
the section around the slide and I let them know they need to turn the slide on 
its side to see the edges of the tissue.  So far, that's helping to cut down on 
 the too-wide tissue sections . 



Aaaahhh...retirement!  I can sit here in the comfort of my own home and make 
snide remarks on Histonet at my leisure!  Cowabunga!  The Joy!  And I still 
have 18 days to go. 
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Re: [Histonet] Shiny side of a paraffin section

[Lee & Peggy Wenk]

I think it's more to have consistency, rather than, say, a physical reason. 
My opinion.


Example:
- Tech A put the shiny side down on the flotation bath, and picked up the 
sections on the slide, and did an H&E.
- Later in the day, the pathologist needs additional levels or some special 
stains or IHC  on the same block.
- If Tech B now cuts the same block and puts shiny side up, the sections 
would be 180 degrees reversed. So if the pathologist saw the area of concern 
in lower left quadrant in the original H&E, now it would be in the upper 
right quadrant.


Sort of the same reason when laying out ribbons, it would be nice for the 
the top of the block be picked up from the ribbon oriented towards the top 
(frosty) part of the slide. If all techs picked up the ribbon in the same 
orientation directions, all subsequent recuts would also be in the same 
direction, regardless of which tech cut the block. (Unless of course you are 
putting 3 ribbons on the same slide, then the top of the block may be 
different, but even then, the ribbons are always laid out in the same 
directions, so that all 3 ribbons of tissue are facing the same direction.)


It just makes it easier for the pathologist to find the same area quickly on 
each section. And for the histotech to check the quality of the staining in 
specific areas on each slide.


Peggy A. Wenk,HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
The opinions expressed are my own, and do not reflect upon Beaumont 
Hospital.


-Original Message- 
From: Eric Hoy

Sent: Tuesday, February 28, 2012 8:36 PM
To: Histonet
Subject: Re: [Histonet] Shiny side of a paraffin section

All of the cells would be face down when you looked at them!

(It's already been a long week!)

Eric Hoy

===
Eric S. Hoy, Ph.D., SI(ASCP)
Clinical Associate Professor
Department of Medical Laboratory Sciences
The University of Texas Southwestern Medical Center
Dallas, Texas
Email: eric@utsouthwestern.edu
===


On 2/28/12 5:56 PM, "Lucie Guernsey"  wrote:


As all of us who cut paraffin know, the underside of each section as it
comes off the blade is shiny. I've always accepted it as a fact that the
shiny side always goes down on the water bath, but I've begun to wonder
why. Is there a specific reason why we're all taught to put the shiny side
down? What would the difference be between a 'properly' collected section
and a rebelliously collected shiny-side up section? Does it even matter?

Thanks!
Lucie

Lucie Guernsey
UC San Diego
lguern...@ucsd.edu
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