Re: [Histonet] Shiny side of a paraffin section
Hello Everyone I have worked in histology for 41 years and the only reason we ever put sections shiny side up is when we want to look at back to back sections: We would take the first section and put it shiny side up in the bath and the next section immediately after would be placed shiny side down. This will give you adjacent sections that will be cut through the same face i.e. if a cell is cut in half the other half will be mirrored on the other section. This can be a very handy tool for comparing IHC staining. Cheers James H Reilly Senior Histology Technician Institute Of Infection, Immunity, Inflammation College Of Medical, Veterinary and Life Sciences University Of Glasgow Room B4/27 Sir Graeme Davies Building 120 University Place Glasgow G12 8TA Tel: +44 141 330 8420/7573 The University of Glasgow is a charity registered in Scotland, charity number SC004401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Congo red
Is it ONE particular case that is giving you problems, or ALL cases of amyloid? Maybe just the control? If the amyloid is a large deposit, that has been in the patient for a long time, the beta pleats can get warped, and the Congo red will be very pale to no staining. In those cases, we: - Use the Auramine-Rhodamine fluorescence scope (hit slide with green light) on the Congo red stained tissue. Congo red amyloid will fluoresce orange against a black background. - Do a crystal violet stain. Amyloid will be pink violet against a blue purple background. - Do a Thioflavin T stain. Use the FITC fluorescence microscope (hit slide with blue light). TFT will fluoresce yellow against a black background. All the amyloid stains have a weakness when it comes to staining amyloid. There isn't one that works all the time. If the Congo red is working fine, then that's the only stain we do. But if the Congo red isn't staining correctly (our control is great, but the patient's tissue is weak to no staining), then we do one or more of the above options. If you need the crystal violet or TFT procedure, let me know. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of my employer. -Original Message- From: Bryan Llewellyn Sent: Thursday, March 01, 2012 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Congo red Bennhold's method is not the easiest to give good results. Try Highmans's method (http://stainsfile.info/StainsFile/stain/amyloid/congohighman.htm). It is much easier to control. Remember that congo red is not an intensely coloured dye and the results are often pale. If your pathologists want a punchier stain try sirius red F3B (NOT 4B), as this is a deeper red than congo red. It can be used in a Highman type procedure as a direct substitute for congo red. It also gives green birefringence, again somewhat darker than congo red. Bryan Llewellyn Cheri Miller wrote: Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak. Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blocking endogenous activities
Peroxidase, the enzyme you want to block with hydrogen peroxide, is a protein and, as such, should be cross-linked by formalin if the tissue is properly sixed. In consequence, you should eliminate the crosslinkage to fully expose the enzyme before blocking it. Therefore, you should do HIER first and block the peroxidase afterwards. On the other hand, many labs do René J. --- On Thu, 3/1/12, Tuyen Nguyen tuyenma...@yahoo.com wrote: From: Tuyen Nguyen tuyenma...@yahoo.com Subject: [Histonet] blocking endogenous activities To: histonet@lists.utsouthwestern.edu Date: Thursday, March 1, 2012, 6:48 PM In my lab, in IHC protocols for most antibodies, hydrogen peroxide is applied before antigen heat retrieval. However, I wonder there is any different result in IHC stain if hydrogen peroxide for blocking endogenous activities is applied after antigen heat retrieval by using steamer or pressure cooker? Thank you, Mai Nguyen Truong Research Associate II Los Angeles, California ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Repair techs for very old Shandon Tissue Processor, (Roosevelt was in office)
I have an old Shandon tissue processor that is so old, they just called in tissue processor, I think Kennedy was in office, and the serial number is -10. THe machine has been donated and passed down and I am having a hard time getting to work. I t worked once, but now it just keeps giving me every kind of error possible. In speaking with Shandon they have helped me to a coup-le of different resets, but all it does is cycle and try and find other modules. THis os one of those procssors that might have been part of a sequence of several processors. It keeps looking for other modules. ANyway, I cannot get it to do anything. DOes anyone know of a tech out there who can work on these Shandon Pathcenteres? Many thanks. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:pat...@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microtomy
We have run into an interesting scenario and wondering what the experts think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! Dorothy Webb, HT (ASCP) This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AKT and pAKT
Has anyone out there done AKT and pAKT antibodies for IHC. I have been working on them for about a month now and they are still not working right. I hope there is someone out there that can help me??? Courtney Pierce IHC Specialist Quintiles Translational RD - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pie...@quintiles.com clinical | commercial | consulting | capital ** IMPORTANT--PLEASE READ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: microtomy
Make sure all levers and parts of the knife holder are tightened securely, but do not overtighten -especially on the blade holding plate F. Long From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L [dorothy.l.w...@healthpartners.com] Sent: Friday, March 02, 2012 1:06 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] microtomy We have run into an interesting scenario and wondering what the experts think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! Dorothy Webb, HT (ASCP) This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] blocking endogenous peroxidase
Some peroxidase-like enzymes in macrophages (probably catalase) and DAB-binding activities in erythrocytes (hemoglobin) are not blocked by formalin. The best way to block this activity was incubation with methanol-peroxide (99 parts of methanol-1 part of 30% peroxide) during de-waxing. 3% peroxide is also effective, but in some cases it causes extensive bubbling that can damage fragile sections. So, I am using methanol-peroxide for paraffin sections and stable peroxide buffer (buffer provided in DAB developer kits, such as Pierce metal-enhanced DAB kit) for cryo- sections. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabsmailto:AGleiberman@cbiolabs.com This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AKT and pAKT
I've used cell signaling 4060S XP for pAKT, and it works really well. From: courtney.pie...@quintiles.com To: histonet@lists.utsouthwestern.edu Date: Fri, 2 Mar 2012 13:31:11 -0500 Subject: [Histonet] AKT and pAKT Has anyone out there done AKT and pAKT antibodies for IHC. I have been working on them for about a month now and they are still not working right. I hope there is someone out there that can help me??? Courtney Pierce IHC Specialist Quintiles Translational RD - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pie...@quintiles.com clinical | commercial | consulting | capital ** IMPORTANT--PLEASE READ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtomy
Cold temperature will shrink the block and determine thinner sections, not thicker sections. The problems has to be on the mechanics or perhaps the block is hotter than you think it is. René J. --- On Fri, 3/2/12, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Subject: [Histonet] microtomy To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Friday, March 2, 2012, 1:06 PM We have run into an interesting scenario and wondering what the experts think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! Dorothy Webb, HT (ASCP) This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] lab. setting up
dear all on histonet I'm going to setup a new histology lab. in Egypt. I need to contact with you to advice me about the best and comfotable facilities microtomes- processors-automatic stainers and so on . Mohamed Ass. Lec. of histology Faculty of Vet. Med. Cairo University Egypt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] lab. setting up
It would be better if you contact a local sales manager knowledgeable of the local characteristics and ask him/her this question. From here you will probably receive advises about instruments that probably are not available in Egypt. René J. --- On Fri, 3/2/12, mohamed abd el razik k8...@yahoo.com wrote: From: mohamed abd el razik k8...@yahoo.com Subject: [Histonet] lab. setting up To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Friday, March 2, 2012, 3:58 PM dear all on histonet I'm going to setup a new histology lab. in Egypt. I need to contact with you to advice me about the best and comfotable facilities microtomes- processors-automatic stainers and so on . Mohamed Ass. Lec. of histology Faculty of Vet. Med. Cairo University Egypt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtomy
Love your question. Hate to hear that you are having a issue. My two cents follow: Yes. The amount of time for a faced block will effect the section thickness. The cells become bloated if they Sit to long. feel free to have a Friday laugh on this one. Anyway. If you havnt changed the stain in any way or the tech isn't rushing and hard facing causing extrenuated cell artifact and you don't have bloated cells? Could it be possible his eyesight got better or maybe he is mad and wants to pick a fuss? I sometimes wish we could post pictures if our issues. I'm wishing you the best. Kim D Sent from my iPhone On Mar 2, 2012, at 1:06 PM, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: We have run into an interesting scenario and wondering what the experts think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! Dorothy Webb, HT (ASCP) This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: PAMS Jones Basement Membrane
A stain I have never had a problem with. However I did have the good fortune to listen to Culling many years ago on the use of 1% periodic acid for PAS staining and he insisted periodic acid, no matter what the concentration, should be made fresh every time you do these stains e.g. PAS and PAMS.Some people use 0.5% but we used 1% for 15 minutes, and then did microwave staining for the methenamine silver.A 56C - 60C water bath also works plus monitoring the color of the sections - the color of dark tea. This is NOT a stable oxidizer once made up, and never should be reused under any circumstances. On the chance you have not used this solution in over 1 1/2 years, you may not be getting proper oxidation of the basement membranes since the periodic acid and the methanamine silver may be bad. Also, the Methenamine silver should be no more than 6 months old. We also kept our silver nitrate solution fresh when we made up the methenamine silver solution, and do store our silver nitrate salts in the refrigerator since this is hygroscopic. Check on storage of this salt in MSDS I have never been one to use kits for either of these stains, particularly with periodic acid as a ready to use solution. This goes into solution very rapidly and be sure to oxidize for 10 minutes before going to the methenamine silver. None of these solutions is difficult to make up in house. If you want, I can send my method and also a protocol pdf from HistoLogic by Stanley Shapiro, where he used freshly made 0.5% periodic acid via private email. The nuclei will pick up some silver, but one should be able to discern nuclei from basement membranes on 1 to 2 µm sections. Good luck Gayle Callis HTL/HT/MT(ASCP) Bozeman MT You wrote: Just wanted to give a quick update on this. I had a suggestion for using thiosemicarbazide for 10 minutes after the periodic acid, and of all the things we tried, this was the only thing that worked. It eliminated the nuclear staining and the capillaries are now picking up the silver (as they should be!). Unfortunately, I accidently deleted the email that suggested this so I don't know who to thank, but it was a great suggestion! Liz From: Elizabeth Cameron Sent: Thursday, February 16, 2012 2:17 PM To: histonet @t lists.utsouthwestern.edu Subject: Jones/PAMS Hi, I was wondering if anyone has any suggestions for a Jones/PAMS stain that is not working properly. This is something we don't do often. The last time we did it was a year and a half ago, and it seemed fine at the time. We have tried 3 or 4 protocols, including an ammoniacal silver, and it is still not working properly. In some protocols, our red cells are staining but the capillaries in the glomeruli do not seem to be picking up the silver. In other protocols, there are nuclei of some cells that should not be staining that are, but again, the capillaries are not. We are working on mouse tissue that is fixed in NBF. The strange thing is the stain seems to be working well on Bouins and Telly's fixed tissue. I even tried mordanting in Bouins! We have tried multiple kidneys with the same results. We are on new bottles of silver and periodic acid, although our methenamine has been around a while. Any suggestions would be greatly appreciated. Thanks! Liz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Posting photographs RE: [Histonet] microtomy
There is a way to post pictures on histonet but just not in the email messaging. The instructions for doing this are found on the Histonet website. Speed does affect the thickness of sections. It pays to turn the flywheel at a steady, slow pace and not NASCAR racing speeds I have sometime observed. Harder paraffin helps for thin sections but one should be able to section at 2 um without difficulty on a properly adjusted microtome, a sharp disposable blade and using any modern day paraffin. Gayle Callis HTL/HT/MT (ASCP) Bozeman MT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Friday, March 02, 2012 2:54 PM To: Webb, Dorothy L Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microtomy Love your question. Hate to hear that you are having a issue. My two cents follow: Yes. The amount of time for a faced block will effect the section thickness. The cells become bloated if they Sit to long. feel free to have a Friday laugh on this one. Anyway. If you havnt changed the stain in any way or the tech isn't rushing and hard facing causing extrenuated cell artifact and you don't have bloated cells? Could it be possible his eyesight got better or maybe he is mad and wants to pick a fuss? I sometimes wish we could post pictures if our issues. I'm wishing you the best. Kim D Sent from my iPhone On Mar 2, 2012, at 1:06 PM, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: We have run into an interesting scenario and wondering what the experts think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! Dorothy Webb, HT (ASCP) This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Multiple stain for epoxy sections
Hello Histonet Users, I have decided that I should ask the experts. I regularly thick section muscle sections in Embed 812 for bouton identification. I normally use Toluidine blue. I am having trouble differentiating between boutons and a type of vacuole that occurs within the same tissue and sometimes same area using TB. Under the LM they can sometimes appear the same. That is until you get it in the TEM and then you have a vacuole and not a bouton. In the Light Microscope the slide under an oil lens doesn't really indicate sub synaptic reticulum other than a darker area. Does anyone know of a multiple stain that can be used for epoxy resin sections (no methanol or Ethanol) that would stain SSR or vesicles a different color from other surrounding areas. I have checked online and indeed there are many stains but from what I read the protocols are for paraffin sections. Surely there is something. Thank you, Lita Duraine EM Technologist Bellen Lab HHMI- Molecular Genetics Duncan Neurological Research Institute 1250 Moursund St. Houston, TX 77030 Rm: N1165.17 MS: N1125.50 832-824-8772 TEM Room 979-549-6526 Cell http://flypush.imgen.bcm.tmc.edu/lab/people/lita.php ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] out of office
I will be out of the office starting 03/02/2012 and will not return until 03/05/2012. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Congo red
From the CAP site: The Benhold Congo red technique does not yield reproducible results and should be avoided and the alkaline Congo red method of Puchtler, et al.,7 remains the gold standard for the demonstration of amyloid in tissue sections. http://www.cap.org/apps/portlets/contentViewer/show.do?printFriendly=truecontentReference=cap_today%2F0609%2F0609g_histologic_preparations.html Jerry From: cmil...@physlab.com To: histonet@lists.utsouthwestern.edu CC: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 1 Mar 2012 08:28:18 -0600 Subject: [Histonet] Congo red Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak. Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
