[Histonet] IHC Position Dallas
IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 4 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 1-2 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. This is an evening position, Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibil...@propath.com Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ohio HT Position
Great part time opportunities' for Histotechnician's in Canton , Ohio! Gastroenterology Associates is looking for certified HT's or HTL's to join their new laboratory . Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT ASCP Certified Duties include: * Grossing * Embedding * Microtomy * Staining * Ability to be flexible and take on additional duties' as needed This is a part time that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@miracals.commailto:mh...@miracals.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.commailto:mh...@miracals.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for a working microtome
Hallo Histo colleagues, I'm posting this for a friend of mine who is looking for a working microtome. He works in an academic lab (San Diego area) and not a lot of money available. Replies from various vendors are welcome as well as individual labs looking to get rid of some of their unused microtomes. thank you and have a good Wednesday Dusko ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Career step ladders in teh Histology lab
I was wondering what career ladders where in your labs? What goals do employees have to meet before they get a pay raise/advancement in the lab/ or a new title? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thickness of slides
Diana- We cut routine slides at 4 microns. Blocks marked for lymph node are cut at 2.5. Prostate needle cores: we cut three levels and save two unstained at each level. HE has two sections - bottom section is always deepest - unstains are taken from in between the upper and lowersection on the HE. Nancy Message: 3 Date: Tue, 18 Sep 2012 14:00:22 -0400 From: Diana McCaig dmcc...@ckha.on.ca Subject: [Histonet] thickness of slides To: histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain; charset=us-ascii Can you tell me what thickness you cut your routine slides for HE and immuno (in particular lymph nodes). Also, your protocol for cutting prostate needle core biopsies.how many spares you cut and do you designate the level on the slide label if multiple levels are submitted on one slide? Thanks Diana NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: thickness of slides
Diana McCaig asks for your protocol for cutting prostate needle core biopsies.how many spares you cut and do you designate the level on the slide label if multiple levels are submitted on one slide? I worked in one of the big prostate labs for a while, and this is what they use, and what I use when can get it. Cut onto slides suitable for immunohistochemistry. Stain slides 1, 3, and 5 with H and E. Hold slides 2 and 4 for possible immunohistochemistry, needed for at least one case in ten. I don't want more than one level on a slide, with a ribbon of at least two. When you embed, by the way, don't put more than two cores in a block, unless the cores are very short. Measure each core and record that in the gross. Make sure you can tell cases apart, since all prostate biopsies look alike in the gross. The big prostate lab used color coding of cassettes, slides, and labels, with a cycle of ten, so that (for example) if the accession number ended in -3 the cassette was always green. Bob Richmond Samurai Pathologist Asbury Place, Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Career step ladders in teh Histology lab
My staff was evaluated annually (on their hiring anniversary) and a salary increase was calculated based on their evaluation results. When a new position was open, in house employees were given preference if their qualification met the new position requirements. If an employee reached a new certification level, they were eligible for any new position open in the next budget. René J. From: Amber McKenzie amber.mcken...@gastrodocs.net To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, September 19, 2012 1:11 PM Subject: [Histonet] Career step ladders in teh Histology lab I was wondering what career ladders where in your labs? What goals do employees have to meet before they get a pay raise/advancement in the lab/ or a new title? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards
Yes, but why take the chance. There are also other chemicals in the lab the picric acid can interact with to make it even more volitile than it was to begin with. Dynamite other explosives have the same problem. The older it gets the more degraded and unstable it becomes. One never knows if or when. I'd like to avoid traumatically amputating my arms if possible, thank you. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Geoff Sent: Mon 9/17/2012 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards I am with Wayne on this one. While I have not tried to make it explode it does seem to me that the dangers are hyped beyond reason. Years ago an old bottle of picric acid would be discovered in a high school chemistry lab. Horrors! Call the bomb squad! So it was taken out to a large field, packed with explosives and BOOM! Of course it exploded, it was surrounded with explosives. Geoff On 9/14/2012 8:58 PM, E. Wayne Johnson wrote: What danger of Picric Acid are you concerned with? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Request for assistance
I have a paper ready for publication, but the reviewers suggested that some photographs should be included. Unfortunately, when I retired I moved to a new community and do not have access to a histology laboratory anymore. Unfortunately, I discarded all the slides I had stained developing the method, stupid I know. Is there anyone who could help and stain half a dozen sections for me. I have a microscope and digital camera so I can take the photos. The method is for visualising aldehydes after oxidation, analagous to a PAS etc. If you can help please contact me at llewl...@shaw.ca or phone 250-245-9825. Bryan Llewellyn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOX10
Hello, Has there been a successful response to the question on SOX10 on Ventana platform? We have very faint labeling on first tries. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Picric Acid
A WEB site just for historical interest: http://en.wikipedia.org/wiki/Halifax_Explosion We continue to use picric acid in the lab, but only as an aqueous or saturated solution. The chemical safety guys came out and carefully removed the bottle of moistened picric acid that we had on the shelf for several years - they were very excited as it was no longer moist - Montana is very dry. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, September 19, 2012 1:17 PM To: Geoff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards Yes, but why take the chance. There are also other chemicals in the lab the picric acid can interact with to make it even more volitile than it was to begin with. Dynamite other explosives have the same problem. The older it gets the more degraded and unstable it becomes. One never knows if or when. I'd like to avoid traumatically amputating my arms if possible, thank you. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Geoff Sent: Mon 9/17/2012 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards I am with Wayne on this one. While I have not tried to make it explode it does seem to me that the dangers are hyped beyond reason. Years ago an old bottle of picric acid would be discovered in a high school chemistry lab. Horrors! Call the bomb squad! So it was taken out to a large field, packed with explosives and BOOM! Of course it exploded, it was surrounded with explosives. Geoff On 9/14/2012 8:58 PM, E. Wayne Johnson wrote: What danger of Picric Acid are you concerned with? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Picric Acid
There is a lot of published data on the hazards of picric acid - although I don't think most histo labs have to worry about what they have in house for trichromes and fixation. It did cost me more disposal since we used to use hundreds of gallons a year for fixation of testes. Finding an alternative fixative was a good move for us. Jackie O' -Original Message- From: Goins, Tresa tgo...@mt.gov To: Ingles Claire cing...@uwhealth.org; Geoff mcaul...@umdnj.edu; histonet histonet@lists.utsouthwestern.edu Sent: Wed, Sep 19, 2012 3:02 pm Subject: [Histonet] Picric Acid A WEB site just for historical interest: http://en.wikipedia.org/wiki/Halifax_Explosion e continue to use picric acid in the lab, but only as an aqueous or saturated olution. The chemical safety guys came out and carefully removed the bottle of moistened picric acid that we had on the shelf for several years - they were ery excited as it was no longer moist - Montana is very dry. Tresa Original Message- rom: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] n Behalf Of Ingles Claire ent: Wednesday, September 19, 2012 1:17 PM o: Geoff; histonet@lists.utsouthwestern.edu ubject: RE: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards Yes, but why take the chance. There are also other chemicals in the lab the icric acid can interact with to make it even more volitile than it was to begin ith. Dynamite other explosives have the same problem. The older it gets the ore degraded and unstable it becomes. One never knows if or when. I'd like to void traumatically amputating my arms if possible, thank you. laire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Geoff ent: Mon 9/17/2012 9:26 AM o: histonet@lists.utsouthwestern.edu ubject: Re: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards I am with Wayne on this one. While I have not tried to make it explode it does eem to me that the dangers are hyped beyond reason. ears ago an old bottle of picric acid would be discovered in a high school hemistry lab. Horrors! Call the bomb squad! So it was taken out to a large ield, packed with explosives and BOOM! Of course it exploded, it was surrounded ith explosives. Geoff On 9/14/2012 8:58 PM, E. Wayne Johnson wrote: What danger of Picric Acid are you concerned with? ___ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Picric Acid
When I was in Texas the second time ( I was there from 1981-1986 and returned in 1991. OK so I'm dating myself) , we were preparing a CAP inspection. I found a brown bottle way in the back of the chemical cabinet. Lo and behold it was the picric acid I saved from the previous go round. I said to my supervisor (Hector for those of you who know us) Hey man, look what I found? Picric acid. We can't have this around, I'll go put in my truck. I put more water in the bottle and I carried it around a couple of days until after the inspection. Yep, miss those days. The things I do for my buddy. Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, September 19, 2012 3:20 PM To: tgo...@mt.gov; cing...@uwhealth.org; mcaul...@umdnj.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Picric Acid There is a lot of published data on the hazards of picric acid - although I don't think most histo labs have to worry about what they have in house for trichromes and fixation. It did cost me more disposal since we used to use hundreds of gallons a year for fixation of testes. Finding an alternative fixative was a good move for us. Jackie O' -Original Message- From: Goins, Tresa tgo...@mt.gov To: Ingles Claire cing...@uwhealth.org; Geoff mcaul...@umdnj.edu; histonet histonet@lists.utsouthwestern.edu Sent: Wed, Sep 19, 2012 3:02 pm Subject: [Histonet] Picric Acid A WEB site just for historical interest: http://en.wikipedia.org/wiki/Halifax_Explosion e continue to use picric acid in the lab, but only as an aqueous or saturated olution. The chemical safety guys came out and carefully removed the bottle of moistened picric acid that we had on the shelf for several years - they were ery excited as it was no longer moist - Montana is very dry. Tresa Original Message- rom: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] n Behalf Of Ingles Claire ent: Wednesday, September 19, 2012 1:17 PM o: Geoff; histonet@lists.utsouthwestern.edu ubject: RE: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards Yes, but why take the chance. There are also other chemicals in the lab the icric acid can interact with to make it even more volitile than it was to begin ith. Dynamite other explosives have the same problem. The older it gets the ore degraded and unstable it becomes. One never knows if or when. I'd like to void traumatically amputating my arms if possible, thank you. laire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Geoff ent: Mon 9/17/2012 9:26 AM o: histonet@lists.utsouthwestern.edu ubject: Re: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards I am with Wayne on this one. While I have not tried to make it explode it does eem to me that the dangers are hyped beyond reason. ears ago an old bottle of picric acid would be discovered in a high school hemistry lab. Horrors! Call the bomb squad! So it was taken out to a large ield, packed with explosives and BOOM! Of course it exploded, it was surrounded ith explosives. Geoff On 9/14/2012 8:58 PM, E. Wayne Johnson wrote: What danger of Picric Acid are you concerned with? ___ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gastro prices
For Gastric Biopsy, I need to know how much other labs are charging for: - HE slide - Unstained slide - Special stain Giemsa and Ab/PAS Thank you very much. Lin Bustamante. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] SOX10
We can't get sox10 to work on anything, let alone with a processor. We use chick tissue though. Emily On Sep 19, 2012 3:57 PM, Michael Kent mk...@dermpathlab.com wrote: Hello, Has there been a successful response to the question on SOX10 on Ventana platform? We have very faint labeling on first tries. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
