RE: [Histonet] Pronase antigen retrieval
Hello Eva, We are not using pronase anymore, but when we did, we were doing it at room temperature. (15 mg in 250 ml PBS, sigma P-5147). It worked fine. Our Trypsin antigen retrieval, we were doing it at 37C as follows: the evening before the retrieval, we put some PBS in the 37C stove, the next morning we dissolved the trypsin in the warm PBS and we put the slides in it (again in the stove, for 15 minutes). Good luck, Willem Van: histonet-boun...@lists.utsouthwestern.edu namens Eva Permaul Verzonden: di 11-9-2012 19:36 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Pronase antigen retrieval Hello out there in Histoland, I have to do some bulk runs with Pronase antigen retrieval. Up until now the number of slides have been so few that I have been able to do the antigen retrieval by dropping pronase on each slide and incubating them in a humidified chamber at 37C for 20min. I now have so many slides that my manager has suggested that I make up large amounts of the Pronase (250ml) and submerge the slides. She suggested placing the container in the oven earlier to allow the Pronase to get to 37C before I add the slides. How long is the Pronase ok to be keep at 37C before I use it? How long in advance can I place it in the 37C before it looses its ability to be used as an antigen retrieval? Thanks for all your help, Eva Permaul HTSR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] methanol vs ethanol in processing
Hello all If tissue (from autopsy cases) has been fixed in 10% formal saline, will there be a difference in the stained section if: processed in methanol processed in ethanol Thanks Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: michelle.perr...@uct.ac.za ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] methanol vs ethanol in processing
Probably not but processing with methanol is a very bad idea. It is highly toxic and tends to make tissues somewhat brittle. René J. From: Michelle Perrins michelle.perr...@uct.ac.za To: histonet@lists.utsouthwestern.edu Sent: Thursday, September 20, 2012 8:46 AM Subject: [Histonet] methanol vs ethanol in processing Hello all If tissue (from autopsy cases) has been fixed in 10% formal saline, will there be a difference in the stained section if: processed in methanol processed in ethanol Thanks Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: michelle.perr...@uct.ac.za ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Immediate Opening for a Histotech at ProPath in Dallas, TX
HISTOTECHNOLOGIST ProPath, a progressive, CAP accredited, high volume, pathology practice, located in Dallas, Texas, is seeking a Histotechnologist. In this position you will be responsible for embedding tissue specimens, microtomy of paraffin-embedded tissue, operation of automated stainer and coverslipper, equipment maintenance and record retention. We require at least 3 years of experience. The ideal candidate will have a high school diploma or equivalent. We prefer, HT, HTL (ASCP) registered or eligible. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit: www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to: accessibil...@propath.com __ Norm Burnham, MBA, MT(ASCP) Director, Anatomic Laboratory Operations ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 norm.burn...@propath.com mailto:norm.burn...@propath.com 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell To learn more about ProPath, please visit: www.propath.com http://www.propath.com/ This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Milestone Histos 5
Does anyone know of a company who buys used Histology equipment and would give quotes for used Milestone Histos 5 microwave tissue processors. Thanks! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
What are the steps to applying and receiving a health care license to work in florida for an HT or an HTL? I heard there is also a requirement or different fees to be paid for a position as supervisor. Curious in Georgia. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Here are some links for HT/HTL licensure in FL. http://ww2.doh.state.fl.us/mqaservices/PractitionerServices.asp http://www.doh.state.fl.us/mqa/ClinLab/clp_applications.html There is a lot of information on that page, not just the application, so you might want to spend a few extra minutes looking around. Further down on the page, you can find the link related to the application. I've made it easier for you, by including the direct link here: http://www.doh.state.fl.us/mqa/ClinLab/ap_licensure.pdf The following URL is one resource for online CEUs. You can take the classes online, print your certificates and mail them in with your application, all on the same day! http://www.4ceuinc.com/home.asp?Profession=1 You will need to create a user id (using your email address is an easy way to remember your user name!) and password in order to see the next link. http://www.4ceuinc.com/courseDetails.asp?CourseId=344 This link takes you to the page for NEW FL licensee people. I would suggest you take the online courses, so you can print your certificate at the end On Sep 20, 2012, at 12:19 PM, Dorothy Glass techman...@yahoo.com wrote: What are the steps to applying and receiving a health care license to work in florida for an HT or an HTL? I heard there is also a requirement or different fees to be paid for a position as supervisor. Curious in Georgia. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How can i post a question in histonet?
You just did. Brett -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of arjun ruhella Sent: Thursday, September 20, 2012 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How can i post a question in histonet? Please let me know! Thank you Best, AR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] looking for old dip-n-dunk processor
I want to find one of the antique tissue processors without any heat or vacuum. Anyone know of one? Thanks, Patty Lott, Laboratory Manager UAB CMBD Core Laboratory ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How can i post a question in histonet?
Please let me know! Thank you Best, AR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How can i post a question in histonet?
Oh, i see. I did not know this is how it works. Thank you. Hope to gain knowledge from this site. Best, AR On Sep 20, 2012, at 1:50 PM, Connolly, Brett M wrote: You just did. Brett -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of arjun ruhella Sent: Thursday, September 20, 2012 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How can i post a question in histonet? Please let me know! Thank you Best, AR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Gastro prices
Hi all, Lin asked about charges for HE slides, unstained slides, etc. for gastric biopsy. Lin, I just want to caution you about asking for this information on an open forum such as this. I'm not up on all the legal issues, but I think you want to avoid any suspicion of price fixing. A source of information might be the reference lab with whom you already do busines. And of course, take into consideration the amount of reimbursement for the test. Thanks, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: cost/slide
Hi, I am also interested in finding average cost of he , ihc and special stains in automated lab setting. Please send response to me directly. Thank you, Kiran Sent from my Verizon Wireless BlackBerry -Original Message- From: D'Attilio, Shelley sdatt...@stormontvail.org Sender: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 20 Sep 2012 12:59:42 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Gastro prices Hi all, Lin asked about charges for HE slides, unstained slides, etc. for gastric biopsy. Lin, I just want to caution you about asking for this information on an open forum such as this. I'm not up on all the legal issues, but I think you want to avoid any suspicion of price fixing. A source of information might be the reference lab with whom you already do busines. And of course, take into consideration the amount of reimbursement for the test. Thanks, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Her 3
Dear Histonetters, Has anyone had success with Dako's Her 3 antibody on paraffin sections? I am looking for a protocol to work on a DAKO autostainer or Ventana XT. I have tried the high ph Dako retrieval suggested but I am not getting much staining and I have ran a multiple tissue control block with many of the suggested controls in the block. I am going to try again using a different antibody diluent because the one I am using may not be compatible with the antibody. Does anyone have any other suggestions. ( I have tried 1:50 for 60 min primary antibody incubation) Elaine Dooley HTL Shands Teaching Hospital Gainesville FL 352-265-0111 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Is specimen too cold for cryosectioning?
Dear all, I have heard a little trick in cryosectioning where, if the specimen is too cold, one put thumb on the specimen and then cut immediately. I have a question related to this. Q1 = My cryostat temperature cannot be increased above -20C (a problem needs to be fixed). I usually tend to cut my specimen at ~-18C. As i was bound to cut the specimen at -20C, i was experiencing chattering or splintering in the sections. I though this was because the specimen was very cold. When i rub my thumb on the specimen for a while, i was able to get 2-4 decent sections, but the chattering started again. Is this a CONCRETE evidence that the chattering is due to specimen being cold? Q2 = Does sectioning difficulty can happen with as small difference as -2C (-18C vs -20C)? I heard ppl saying that -2C will not make any difference in sectioning. Hope to hear from someone soon! Sincere thank you! AR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Is specimen too cold for cryosectioning?
Putting you thumb over the specimen will slightly increase its temperature but I do not think will improve how it cuts, besides you being exposed to whatever may be in the specimen, that is unfixed. Too cold specimens will be tamed by changing the blade angle and the speed of sectioning. A small difference of ±2ºC should not have any effect. You have also to remember that friction from sectioning will alter the temperature of the specimen, as well as sectioning with the cryostat door open. Ability to section is the key. René J. From: arjun ruhella ruhella.ar...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, September 20, 2012 2:39 PM Subject: [Histonet] Is specimen too cold for cryosectioning? Dear all, I have heard a little trick in cryosectioning where, if the specimen is too cold, one put thumb on the specimen and then cut immediately. I have a question related to this. Q1 = My cryostat temperature cannot be increased above -20C (a problem needs to be fixed). I usually tend to cut my specimen at ~-18C. As i was bound to cut the specimen at -20C, i was experiencing chattering or splintering in the sections. I though this was because the specimen was very cold. When i rub my thumb on the specimen for a while, i was able to get 2-4 decent sections, but the chattering started again. Is this a CONCRETE evidence that the chattering is due to specimen being cold? Q2 = Does sectioning difficulty can happen with as small difference as -2C (-18C vs -20C)? I heard ppl saying that -2C will not make any difference in sectioning. Hope to hear from someone soon! Sincere thank you! AR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Opening Near Fort Myers, FL
Allied Search Partners is currently looking for a qualified applicant for a Histotechnician or Histotechnologist in southwest FL. Message me for details. FL Technician or Technologist licensed Previous experience with automated IHC Technical and QC protocols AA or BS/BA degree in life science preferred but not required 3+ years experience Benefits: Competitive salaries, Health, Dental, Life Disability insurances, a section 125 plan, a 401K, FSA, ESPP and relocation assistance. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Is specimen too cold for cryosectioning?
Hi Arjun, In my experience, 2 degrees C can make a huge difference in the ability to section something well. We have a unit that can set the specimen/object temperature separately from the chamber temp and when having difficulty I start making temperature adjustments 1-2 degrees at a time. I think that cutting temperature is too cold when your tissue sample shatters when sectioning (depending too on the thickness). If you can rub your thumb across it and you get a few decent sections, the warming of the specimen likely explains it. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Milestone Histos 5
IMEB buys and refurbishes equipment for resale. Sent from the iPhone of Kim Tournear On Sep 20, 2012, at 11:15 AM, histot...@imagesbyhopper.com histot...@imagesbyhopper.com wrote: Does anyone know of a company who buys used Histology equipment and would give quotes for used Milestone Histos 5 microwave tissue processors. Thanks! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Picric acid
Hi, The Halifax explosion was indeed a very dramatic event. Anyone unfamiliar with the story should certainly read up on it. It was truly incredible. The link to the Wikipedia article was previously posted. A couple of important points about this story. The ship that blew up was carrying metric *tons* of picric acid. The ship also was carrying tons of other explosive material (nitroglycerine amongst others). If your lab has tons of picric acid (not 10-100 grams like most labs) and tons of other explosives, you might have cause for panic. If not, you likely have an extremely small amount stored under water or in a solution which poses less risk when used stored properly than many other chemicals in a lab. I don't mean to say there is no risk, but I would say the concern is a bit overly dramatic. Like a carpenter, know your tools and how to treat them and they will serve you well. Otherwise ANY of the tools you have are likely to bite you. Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] burned tissue artifact: need help
Hi all, Due to water contamination on the processor some of our cases had processing artifact with poor histology. Any suggestion to remedy this issue? We did reprocess the blocks but still not good. Any cutting or staining tips so slides can be readable. Thank you all, Kiran Sent from my Verizon Wireless BlackBerry -Original Message- From: Amos Brooks amosbro...@gmail.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 20 Sep 2012 17:54:04 To: histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: [Histonet] Picric acid Hi, The Halifax explosion was indeed a very dramatic event. Anyone unfamiliar with the story should certainly read up on it. It was truly incredible. The link to the Wikipedia article was previously posted. A couple of important points about this story. The ship that blew up was carrying metric *tons* of picric acid. The ship also was carrying tons of other explosive material (nitroglycerine amongst others). If your lab has tons of picric acid (not 10-100 grams like most labs) and tons of other explosives, you might have cause for panic. If not, you likely have an extremely small amount stored under water or in a solution which poses less risk when used stored properly than many other chemicals in a lab. I don't mean to say there is no risk, but I would say the concern is a bit overly dramatic. Like a carpenter, know your tools and how to treat them and they will serve you well. Otherwise ANY of the tools you have are likely to bite you. Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
