[Histonet] Re: Reliable... Hi Bill (William DeSalvo), Let's try this: A Newbie's Guide to Histonet
Hi Histocare and any other relatively new people to Histonet, First, hello from Bill and I in beautiful Vancouver and NSH where many histologists are enjoying old friends and making new friends from around the world. Let's remember histology is still a pretty small field in the US with about 25,000 histologists working in about 7,000 labs, plus more and more working in research labs and companies. Over a career, you're likely to meet many of them if you come to enough NSHs. For those of us who have been on Histonet since the beginning (1996?), we would like to remind others of the facts of Histonet: The Histonet listserver is an email listserver for the histology profession that is managed by Dr. Margraf and Dr. Cope-Yokoyama and is run on computers at the University of Texas Southwestern Medical Center. University policies prohibit advertising, but do allow posting of jobs, probably so everybody can dream about being a histologist in some distant place. There are even some temp positions and jobs wanted emails posted every now and then. Histocare, your first posting was no problem and we enjoy seeing all the ways histologists use to market their talents. Three posts in one week is a little redundant. We got it the first time. Companies (Vendors) are permitted to post in response to problems of labs when they have something positive to contribute. Histonet currently has more than 4000 members from throughout the world, with many thousands more who keep up with it through the archives. The archives have over 30,000 visits a month from over 50 different countries. We all want to read questions and answers about problems in histology. Many of us remember before Histonet when labs had to actually solve their problems by themselves. Now over 30,000 times a month a histology problem is solved by one of the eloquent answers of contributors to Histonet. And Histocare, you can be anonymous on Histonet if you like, but you might want to search the archives for others opinions on it. It has been discussed previously. And when you have a website, you can't be anonymous, because I was able to do a whois search and get your name and address. I would recommend using your name and credentials proudly. So, let's get back to solving histology problems on Histonet and leave Dr. Margraf and Dr. Cope-Yokoyama alone. They have patients and stuff they're working on. Just remember to treat others in your profession with respect on Histonet. You just might meet them one day at NSH. And remember to think twice (or three times) before hitting the send button with a negative message. Thousands of us really don't want to hear it. Histonet welcomes all histology questions and a vast majority of the participants think if you don't know the answer, it's not a dumb question, so feel free to ask. Those that don't think so will flame you mercilessly off list for posting, but I recommend you ignore them. A little research in the archives shows me that 94% of the time an email war breaks out on Histonet, testosterone is involved... Respectfully, Marvin Hanna webmas...@histosearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Reliable... Hi Bill (William DeSalvo), Let's try this: A Newbie's Guide to Histonet
Hi, and thanks for the intervention! :) This is the first bit of rational and non-confrontational communication relating to advertising I've received thus far and appreciate this very much Marvin. I'll respectfully honor the intention of histonet. Had I been approached with respect and asked nicely I would gladly oblige. You know what they say about catching more flies with honey... Now back to our regularly scheduled histology programming. On Oct 3, 2012, at 2:16 AM, Marvin Hanna mha...@histosearch.com wrote: Hi Histocare and any other relatively new people to Histonet, First, hello from Bill and I in beautiful Vancouver and NSH where many histologists are enjoying old friends and making new friends from around the world. Let's remember histology is still a pretty small field in the US with about 25,000 histologists working in about 7,000 labs, plus more and more working in research labs and companies. Over a career, you're likely to meet many of them if you come to enough NSHs. For those of us who have been on Histonet since the beginning (1996?), we would like to remind others of the facts of Histonet: The Histonet listserver is an email listserver for the histology profession that is managed by Dr. Margraf and Dr. Cope-Yokoyama and is run on computers at the University of Texas Southwestern Medical Center. University policies prohibit advertising, but do allow posting of jobs, probably so everybody can dream about being a histologist in some distant place. There are even some temp positions and jobs wanted emails posted every now and then. Histocare, your first posting was no problem and we enjoy seeing all the ways histologists use to market their talents. Three posts in one week is a little redundant. We got it the first time. Companies (Vendors) are permitted to post in response to problems of labs when they have something positive to contribute. Histonet currently has more than 4000 members from throughout the world, with many thousands more who keep up with it through the archives. The archives have over 30,000 visits a month from over 50 different countries. We all want to read questions and answers about problems in histology. Many of us remember before Histonet when labs had to actually solve their problems by themselves. Now over 30,000 times a month a histology problem is solved by one of the eloquent answers of contributors to Histonet. And Histocare, you can be anonymous on Histonet if you like, but you might want to search the archives for others opinions on it. It has been discussed previously. And when you have a website, you can’t be anonymous, because I was able to do a whois search and get your name and address. I would recommend using your name and credentials proudly. So, let’s get back to solving histology problems on Histonet and leave Dr. Margraf and Dr. Cope-Yokoyama alone. They have patients and stuff they’re working on. Just remember to treat others in your profession with respect on Histonet. You just might meet them one day at NSH. And remember to think twice (or three times) before hitting the send button with a negative message. Thousands of us really don’t want to hear it. Histonet welcomes all histology questions and a vast majority of the participants think if you don’t know the answer, it’s not a dumb question, so feel free to ask. Those that don’t think so will flame you mercilessly off list for posting, but I recommend you ignore them. A little research in the archives shows me that 94% of the time an email war breaks out on Histonet, testosterone is involved… Respectfully, Marvin Hanna webmas...@histosearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana discontinuing INFORM HPV product line
Hi all, We have just been informed that Ventana is discontinuing the INFORM HPV Family 6 ASR and the Family 16 Probe ASR. I believe that Ventana is the only company that offers this kind of product. We don't have any experience in our laboratory developing and testing our own cocktails. Can someone offer some words of wisdom or perhaps a procedure? At this point, I don't really know where to start other than to find out the probes included in the Family 16 ASR and to buy some dispensers for the BenchMark XT and Ultra. Ventana cannot help us due to FDA regulations regarding laboratory developed tests (I think). Thanks, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Reliable Histology Team Member to embed and cut for you.
Well, if I had ever thought of using your services in the past, after reading this post I would never consider it. You should have quit responding a couple of posts ago. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Tuesday, October 02, 2012 7:49 PM To: Jay Lundgren Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reliable Histology Team Member to embed and cut for you. Ok, let me put this issue to bed. It's fine you feel you need to be the voice for those who have all the help they need and will never need the services of a histology professional to help cover staffing shortages or inadequate/ineffective staffing. There are thousands of subscribers to this list and I respect every single one of them. HistoCare is for those who NEED professional assistance on an interim basis. And let's be clear, I'm not just a tech looking for job once in a while. I'm a professional extending my expertise and invaluable abilities to help when the need arises on relatively short notice without compromising patient care. Your messages seem to have a very petty tone and not respectful of the nature of the work we do. It's almost as if you are not even of this profession. I care about all those people who go to doctor to have a million tests done to see what's wrong with them and have to wait days and weeks before they get any kind of news to settle their nerves or some glimmer of hope. I want to minimize that inconvenience as much as I can. I am a real person first, a histology professional second on the other side of this email just like there is a real patient on the other side of that slide. You only care about HistoCare advertising on one of the few forums histology professionals have to exchange thoughts, ideas, and RESOURCES. If the best you can add to histonet is criticism, it doesn't need you or those like you. This is a small community with very high turnover, low job satisfaction, some employed individuals with marginal or inadequate abilities, and little interest in others to want to break into this profession. Heck even the ones that's been his field for a long time express dismay, let alone the newbies who can't get the proper support from their own supervisors! Good grief! Those who rely on histonet for advice and ideas should also be able to search for dependable lab support and hope that there is a good resource for them to call upon. Please do me a favor and respectfully bow out and resist the urge to respond further as I have no interest in debating. I'd rather spend my time being productive. There isn't a policy specifically stating i can't make HistoCare available for those who may search for assistance. Lets not forget Histonet is a courtesy to us all for interests in histology and what it is not, is a vehicle to complain. I was initially open to your suggestions for alternatives and offered you the opportunity to respond with solutions acceptable to 'you' but I see you would rather be a complainer than a helper. Any future responses from you referring to this matter directly or indirectly will be considered harassment and forwarded to the appropriate parties. Sincerely HistoCare www.HistoCare.com On Oct 2, 2012, at 4:19 PM, Jay Lundgren jaylundg...@gmail.com wrote: I am not the only forum member who is concerned about this. I have received private messages from others who agree with me and choose not to reply to all. I will not repost them out of respect for their privacy. The facts that you take what you do seriously, or are not disrespectful are moot. The fact that does apply here is that advertising is not allowed on Histonet. The staffing agencies that occasionally post on here are offering a list of open jobs. You are soliciting for your services, three times in one week. (9/25, 9/26, 10/1) Histonet is not a forum for marketing, pitching, plugging, promulgating or selling. I hope it stays that way. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Reliable Histology Team Member to embed and cut for you.
What was the initial purpose for starting the Histonet, I assume it was to offer a forum for Histotech to get assistance with problems they may encounter. It offends me when there are those that feel they can control what is posted, the histonet is for the group not a few individuals. Histocare may be excessive in their postings but if it does not apply to you don't read it. They are no different then the other staffing agencies posting job listing because in turn they are promoting their company. Both provide a service, again if you don't need their service don't read it. However someone may need their services and they should not be excluded. Just my two cents worth! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lori Harris Sent: Wednesday, October 03, 2012 8:12 AM To: Contact HistoCare; Jay Lundgren Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reliable Histology Team Member to embed and cut for you. Well, if I had ever thought of using your services in the past, after reading this post I would never consider it. You should have quit responding a couple of posts ago. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Tuesday, October 02, 2012 7:49 PM To: Jay Lundgren Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reliable Histology Team Member to embed and cut for you. Ok, let me put this issue to bed. It's fine you feel you need to be the voice for those who have all the help they need and will never need the services of a histology professional to help cover staffing shortages or inadequate/ineffective staffing. There are thousands of subscribers to this list and I respect every single one of them. HistoCare is for those who NEED professional assistance on an interim basis. And let's be clear, I'm not just a tech looking for job once in a while. I'm a professional extending my expertise and invaluable abilities to help when the need arises on relatively short notice without compromising patient care. Your messages seem to have a very petty tone and not respectful of the nature of the work we do. It's almost as if you are not even of this profession. I care about all those people who go to doctor to have a million tests done to see what's wrong with them and have to wait days and weeks before they get any kind of news to settle their nerves or some glimmer of hope. I want to minimize that inconvenience as much as I can. I am a real person first, a histology professional second on the other side of this email just like there is a real patient on the other side of that slide. You only care about HistoCare advertising on one of the few forums histology professionals have to exchange thoughts, ideas, and RESOURCES. If the best you can add to histonet is criticism, it doesn't need you or those like you. This is a small community with very high turnover, low job satisfaction, some employed individuals with marginal or inadequate abilities, and little interest in others to want to break into this profession. Heck even the ones that's been his field for a long time express dismay, let alone the newbies who can't get the proper support from their own supervisors! Good grief! Those who rely on histonet for advice and ideas should also be able to search for dependable lab support and hope that there is a good resource for them to call upon. Please do me a favor and respectfully bow out and resist the urge to respond further as I have no interest in debating. I'd rather spend my time being productive. There isn't a policy specifically stating i can't make HistoCare available for those who may search for assistance. Lets not forget Histonet is a courtesy to us all for interests in histology and what it is not, is a vehicle to complain. I was initially open to your suggestions for alternatives and offered you the opportunity to respond with solutions acceptable to 'you' but I see you would rather be a complainer than a helper. Any future responses from you referring to this matter directly or indirectly will be considered harassment and forwarded to the appropriate parties. Sincerely HistoCare www.HistoCare.com On Oct 2, 2012, at 4:19 PM, Jay Lundgren jaylundg...@gmail.com wrote: I am not the only forum member who is concerned about this. I have received private messages from others who agree with me and choose not to reply to all. I will not repost them out of respect for their privacy. The facts that you take what you do seriously, or are not disrespectful are moot. The fact that does apply here is that advertising is not allowed on Histonet. The staffing agencies that occasionally post on here are offering a list of open jobs. You are soliciting for your services, three times in one week. (9/25,
Re: [Histonet] Reliable Histology Team Member to embed and cut for you.
I'm sorry you feel that way but my responses have been respectful and courteous even as I have been attacked for making a service available to those who need it. If my advertising was not of interest to anyone in particular it is easy just to ignore. I'm just not understanding how someone can tear down a person for mentioning an available service and in the course of defending my business I get backlash. I didn't ask to be singled out in a derogatory manner on a public forum. And I should have allowed someone to throw their personal fit at me for the second time whom I had absolutely no ill feelings toward? I just don't get this at all. This could have been handled easily and privately by respectfully being asked if I was aware of the histonet 'custom' of not advertising and that it would be frowned upon since this isn't a forum for commercial interests. I would have relented and moved on. On Oct 3, 2012, at 8:11 AM, Lori Harris lhar...@samhealth.org wrote: Well, if I had ever thought of using your services in the past, after reading this post I would never consider it. You should have quit responding a couple of posts ago. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Tuesday, October 02, 2012 7:49 PM To: Jay Lundgren Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reliable Histology Team Member to embed and cut for you. Ok, let me put this issue to bed. It's fine you feel you need to be the voice for those who have all the help they need and will never need the services of a histology professional to help cover staffing shortages or inadequate/ineffective staffing. There are thousands of subscribers to this list and I respect every single one of them. HistoCare is for those who NEED professional assistance on an interim basis. And let's be clear, I'm not just a tech looking for job once in a while. I'm a professional extending my expertise and invaluable abilities to help when the need arises on relatively short notice without compromising patient care. Your messages seem to have a very petty tone and not respectful of the nature of the work we do. It's almost as if you are not even of this profession. I care about all those people who go to doctor to have a million tests done to see what's wrong with them and have to wait days and weeks before they get any kind of news to settle their nerves or some glimmer of hope. I want to minimize that inconvenience as much as I can. I am a real person first, a histology professional second on the other side of this email just like there is a real patient on the other side of that slide. You only care about HistoCare advertising on one of the few forums histology professionals have to exchange thoughts, ideas, and RESOURCES. If the best you can add to histonet is criticism, it doesn't need you or those like you. This is a small community with very high turnover, low job satisfaction, some employed individuals with marginal or inadequate abilities, and little interest in others to want to break into this profession. Heck even the ones that's been his field for a long time express dismay, let alone the newbies who can't get the proper support from their own supervisors! Good grief! Those who rely on histonet for advice and ideas should also be able to search for dependable lab support and hope that there is a good resource for them to call upon. Please do me a favor and respectfully bow out and resist the urge to respond further as I have no interest in debating. I'd rather spend my time being productive. There isn't a policy specifically stating i can't make HistoCare available for those who may search for assistance. Lets not forget Histonet is a courtesy to us all for interests in histology and what it is not, is a vehicle to complain. I was initially open to your suggestions for alternatives and offered you the opportunity to respond with solutions acceptable to 'you' but I see you would rather be a complainer than a helper. Any future responses from you referring to this matter directly or indirectly will be considered harassment and forwarded to the appropriate parties. Sincerely HistoCare www.HistoCare.com On Oct 2, 2012, at 4:19 PM, Jay Lundgren jaylundg...@gmail.com wrote: I am not the only forum member who is concerned about this. I have received private messages from others who agree with me and choose not to reply to all. I will not repost them out of respect for their privacy. The facts that you take what you do seriously, or are not disrespectful are moot. The fact that does apply here is that advertising is not allowed on Histonet. The staffing agencies that occasionally post on here are offering a list of open jobs. You are soliciting for your services,
[Histonet] RE: Ventana discontinuing INFORM HPV product line
After talking with my rep, it sounds like they are trying to come up with an offering next year that will help users replace the test. You can stock up on probe for now. I got a nice discount on a bulk order of probe that will last me until Fall of 2013. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Wednesday, October 03, 2012 8:31 AM To: Histonet Listserv (E-mail) Subject: [Histonet] Ventana discontinuing INFORM HPV product line Hi all, We have just been informed that Ventana is discontinuing the INFORM HPV Family 6 ASR and the Family 16 Probe ASR. I believe that Ventana is the only company that offers this kind of product. We don't have any experience in our laboratory developing and testing our own cocktails. Can someone offer some words of wisdom or perhaps a procedure? At this point, I don't really know where to start other than to find out the probes included in the Family 16 ASR and to buy some dispensers for the BenchMark XT and Ultra. Ventana cannot help us due to FDA regulations regarding laboratory developed tests (I think). Thanks, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Reliable... Hi Bill (William DeSalvo), Let's try this: A Newbie's Guide to Histonet
Marvin, you are always a calming and steady force. Exchange, learn, grow and share with respect. . . Histonet William DeSalvo, B.S., HTL(ASCP) Owner/Consultant, Collaborative Advantage Consulting Date: Wed, 3 Oct 2012 03:16:05 -0400 From: mha...@histosearch.com To: wdesalvo@outlook.com CC: cont...@histocare.com; jaylundg...@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: Reliable... Hi Bill (William DeSalvo), Let's try this: A Newbie's Guide to Histonet Hi Histocare and any other relatively new people to Histonet, First, hello from Bill and I in beautiful Vancouver and NSH where many histologists are enjoying old friends and making new friends from around the world. Let's remember histology is still a pretty small field in the US with about 25,000 histologists working in about 7,000 labs, plus more and more working in research labs and companies. Over a career, you're likely to meet many of them if you come to enough NSHs. For those of us who have been on Histonet since the beginning (1996?), we would like to remind others of the facts of Histonet: The Histonet listserver is an email listserver for the histology profession that is managed by Dr. Margraf and Dr. Cope-Yokoyama and is run on computers at the University of Texas Southwestern Medical Center. University policies prohibit advertising, but do allow posting of jobs, probably so everybody can dream about being a histologist in some distant place. There are even some temp positions and jobs wanted emails posted every now and then. Histocare, your first posting was no problem and we enjoy seeing all the ways histologists use to market their talents. Three posts in one week is a little redundant. We got it the first time. Companies (Vendors) are permitted to post in response to problems of labs when they have something positive to contribute. Histonet currently has more than 4000 members from throughout the world, with many thousands more who keep up with it through the archives. The archives have over 30,000 visits a month from over 50 different countries. We all want to read questions and answers about problems in histology. Many of us remember before Histonet when labs had to actually solve their problems by themselves. Now over 30,000 times a month a histology problem is solved by one of the eloquent answers of contributors to Histonet. And Histocare, you can be anonymous on Histonet if you like, but you might want to search the archives for others opinions on it. It has been discussed previously. And when you have a website, you can’t be anonymous, because I was able to do a whois search and get your name and address. I would recommend using your name and credentials proudly. So, let’s get back to solving histology problems on Histonet and leave Dr. Margraf and Dr. Cope-Yokoyama alone. They have patients and stuff they’re working on. Just remember to treat others in your profession with respect on Histonet. You just might meet them one day at NSH. And remember to think twice (or three times) before hitting the send button with a negative message. Thousands of us really don’t want to hear it. Histonet welcomes all histology questions and a vast majority of the participants think if you don’t know the answer, it’s not a dumb question, so feel free to ask. Those that don’t think so will flame you mercilessly off list for posting, but I recommend you ignore them. A little research in the archives shows me that 94% of the time an email war breaks out on Histonet, testosterone is involved… Respectfully, Marvin Hanna webmas...@histosearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fibronectin Histology
Hello, I have been staining scaffold material for ECM components. Currently I've been using Movat pentachrome and Masson's trichrome. I was wondering, does fibronectin turn a particular color using these staining protocols? Also, is there a histochemical way of staining for fibronectin (some sort of dye) or can it only be done with fibronectin specific antibodies (IHC)? Much Thanks, _ Alicia A. Blancas, PhD Grande-Allen Lab Rice University BRC 217 Email: ablan...@rice.edu _ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 107, Issue 5
Dear Histonetters: Thanks, Marvin for a great summary of Histonet. (It is copied below if anyone didn't get to read it ). For those of you who don't know, Marvin has compiled the Histonet archive for many, many years on his Histosearch website. It is a great, searchable data source. Thanks so much Marvin, for that, too. Dr. Cope and I serve as the administrators for Histonet but it really is all the contributors that make it a worthwhile venture. We are both pediatric pathologist, by the way, so we really do have a lot of patient care responsibilities too. Do be patient if it takes us a few days to get back to you if you email us with questions or concerns. Best wishes, Linda M Histonet administrator Date: Wed, 3 Oct 2012 03:16:05 -0400 From: mha...@histosearch.com To: wdesalvo@outlook.com CC: cont...@histocare.com; jaylundg...@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: Reliable... Hi Bill (William DeSalvo), Let's try this: A Newbie's Guide to Histonet Hi Histocare and any other relatively new people to Histonet, First, hello from Bill and I in beautiful Vancouver and NSH where many histologists are enjoying old friends and making new friends from around the world. Let's remember histology is still a pretty small field in the US with about 25,000 histologists working in about 7,000 labs, plus more and more working in research labs and companies. Over a career, you're likely to meet many of them if you come to enough NSHs. For those of us who have been on Histonet since the beginning (1996?), we would like to remind others of the facts of Histonet: The Histonet listserver is an email listserver for the histology profession that is managed by Dr. Margraf and Dr. Cope-Yokoyama and is run on computers at the University of Texas Southwestern Medical Center. University policies prohibit advertising, but do allow posting of jobs, probably so everybody can dream about being a histologist in some distant place. There are even some temp positions and jobs wanted emails posted every now and then. Histocare, your first posting was no problem and we enjoy seeing all the ways histologists use to market their talents. Three posts in one week is a little redundant. We got it the first time. Companies (Vendors) are permitted to post in response to problems of labs when they have something positive to contribute. Histonet currently has more than 4000 members from throughout the world, with many thousands more who keep up with it through the archives. The archives have over 30,000 visits a month from over 50 different countries. We all want to read questions and answers about problems in histology. Many of us remember before Histonet when labs had to actually solve their problems by themselves. Now over 30,000 times a month a histology problem is solved by one of the eloquent answers of contributors to Histonet. And Histocare, you can be anonymous on Histonet if you like, but you might want to search the archives for others opinions on it. It has been discussed previously. And when you have a website, you can?t be anonymous, because I was able to do a whois search and get your name and address. I would recommend using your name and credentials proudly. So, let?s get back to solving histology problems on Histonet and leave Dr. Margraf and Dr. Cope-Yokoyama alone. They have patients and stuff they?re working on. Just remember to treat others in your profession with respect on Histonet. You just might meet them one day at NSH. And remember to think twice (or three times) before hitting the send button with a negative message. Thousands of us really don?t want to hear it. Histonet welcomes all histology questions and a vast majority of the participants think if you don?t know the answer, it?s not a dumb question, so feel free to ask. Those that don?t think so will flame you mercilessly off list for posting, but I recommend you ignore them. A little research in the archives shows me that 94% of the time an email war breaks out on Histonet, testosterone is involved? Respectfully, Marvin Hanna webmas...@histosearch.com -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 107, Issue 5
[Histonet] CK AE1/AE3
Hi Everyone, I'd like some input as to what labs are using as a procedure for this antibody. Is enzyme digestion or a form of antigen retrieval with heat used? What dilution and detection system do you use as well as what type of machine is it run on? Thanks for the info, Jean Taylor, HT (ASCP) QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: [IHCRG] RE: CK AE1/AE3
We do not use AE1/AE3 anymore. OSCAR single clone is a much better antibody. We retired AE1/AE3 out of fear that it will lead to false negative results in sentinel nodes. Hadi On Oct 3, 2012, at 3:50 PM, Jackie Ferracone wrote: Nice antibody, we use Proteinase-K, at a dilution of 1:500. Detection is with DAKO’s ENV+ , and we use a DAKO autostainer. Jacqueline D. Ferracone Research Associate, Pathobiology University of Pennsylvania/New Bolton Center Veterinary Medicine 382 West Street Road Kennett Square, PA, 19348-1692 610-925-6391 610-350-8860 (cell) 610-925-6810 (fax) From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Taylor, Jean Sent: Wednesday, October 03, 2012 3:47 PM To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [IHCRG] CK AE1/AE3 Hi Everyone, I’d like some input as to what labs are using as a procedure for this antibody. Is enzyme digestion or a form of antigen retrieval with heat used? What dilution and detection system do you use as well as what type of machine is it run on? Thanks for the info, Jean Taylor, HT (ASCP) QIHC IHC Tech Meriter Health Services Madison, WI -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: hi...@nsh.org or call 443.535.4060. -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: hi...@nsh.org or call 443.535.4060. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
